Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM)

The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to optimize the extraction process. The best possible range for methanol concentration (0–100%), the liquid-solid ratio (5:1–30:1 mL/g), ultrasonic power (240–540 W), duration of ultrasound (10–50 min), ultrasonic temperature (10–60 °C), and the number of extractions (1–4) were obtained according to the single factor experiments. Then, using the Box-Behnken design (BBD) of response surface analysis, the optimum extraction conditions were obtained with 16.33% methanol concentration, the liquid-solid ratio of 10.83:1 mL/g and 35.00 °C ultrasonic temperature. Under these conditions, kinsenoside extraction yield reached 32.24% dry weight. The best conditions were applied to determine the kinsenoside content in seven different cultivation ages in Anoectochilus roxburghii.

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Ultrasound-Assisted Extraction of Total Flavonoids from Corn Silk and Their Antioxidant Activity

Journal of Chemistry ◽

10.1155/2016/8768130 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Ling-Li Zheng ◽

Guan Wen ◽

Min-Yong Yuan ◽

Feng Gao

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Antioxidant Activities ◽

Total Flavonoids ◽

Ultrasonic Power ◽

Ultrasound Assisted Extraction ◽

Antioxidant Power ◽

Corn Silk ◽

Assisted Extraction ◽

Ultrasound Assisted

Object. Ultrasound-assisted extraction of total flavonoids from corn silk and their antioxidant activities were studied.Methods. Response surface methodology was adopted to optimize the extraction conditions and antioxidant activities of the extracted total flavonoids were detected through ferric reducing antioxidant power (FRAP) assay.Results. Through a three-level, three-variable Box-Behnken design of response surface methodology (RSM) adopting yield as response, the optimal conditions were determined as follows: ultrasonic power 500 W, extraction time 20 min, material solvent ratio 1 : 20, and ethanol concentration 30%. Under the optimum conditions, the extraction yield of total flavonoids was 1.13%. FRAP value of total flavonoids extracted from corn silk was 467.59 μmol/L.Conclusion. The total flavonoids of corn silk could be developed as food natural antioxidant reagents.

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Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

Applied Sciences ◽

10.3390/app10020560 ◽

2020 ◽

Vol 10 (2) ◽

pp. 560

Author(s):

Gregorio Iván Peredo Pozos ◽

Mario Alberto Ruiz-López ◽

Juan Francisco Zamora Nátera ◽

Carlos Álvarez Moya ◽

Lucia Barrientos Ramírez ◽

...

Keyword(s):

Antioxidant Activity ◽

Human Lymphocytes ◽

Dry Weight ◽

Extraction Process ◽

Hibiscus Sabdariffa ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Antigenotoxic Effect

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

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Optimization of Ultrasound-Assisted Extraction of Oil from Camellia(Camellia oleifera abel) Seed

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.1854 ◽

2011 ◽

Vol 236-238 ◽

pp. 1854-1858 ◽

Cited By ~ 2

Author(s):

Xue Hui Wu ◽

Li Li

Keyword(s):

Ultrasonic Treatment ◽

Treatment Time ◽

Treatment Temperature ◽

Ultrasonic Power ◽

Ultrasound Assisted Extraction ◽

Camellia Oil ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Main Component

Camellia oil is the main component of the camellia seed and has many beneficial functions to human health. An investigation into ultrasound- assisted extraction was conducted for the oil extraction from camellia seed. The optimized condition of extraction parameters obtained with the response surface methodology (RSM) at a four-variable, three-level experiment Box–Behnken design (BBD), was as follows: liquid-solid ratio, 5mL/g;ultrasonic power,155W;ultrasonic treatment time,31 min; and ultrasonic treatment temperature, 55°C, under these conditions, the oil yeild was 44.55%. GC–MS analysis showed that the content of unsaturated fatty acid in camellia oil was up to 84.47% and the content of oleic acid was over 76.15%.

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A ULTRASOUND-ASSISTED EXTRACTION (UAE) OF PHYTOCHEMICALS WITH RESPONSE SURFACE METHODOLOGY(RSM) IN Curcuma xanthorrhiza

Science Proceedings Series ◽

10.31580/sps.v2i2.1431 ◽

2020 ◽

Vol 2 (2) ◽

pp. 139-146

Author(s):

Najiba Azemi ◽

Norazah Basar ◽

Susilawati Toemen

Keyword(s):

Response Surface Methodology ◽

Optimum Condition ◽

Response Surface ◽

Extraction Process ◽

Extraction Time ◽

Array Detector ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted

The Curcuma genus belongs to the Zingiberaceae family is commonly known for its usage as spice and medicine.it is widely used in pharmaceutical, food supplement, cosmetic industries lead to economic benefits. Recently, noticing to an eco-friendly extraction approach which provides the extraction efficiency is considered that in the extraction process, exploring the optimum condition of extraction parameters. This study is performed to improve the bioactive compounds extraction from C. xanthorrhiza by ultrasound-assisted extraction (UAE) through Response Surface Methodology (RSM). Three independent variables: extraction time (5-20 min), temperature (30-50 ° C) and liquid-solid ratio (6-10 mL / g) are applied using Box-Behnken (BBD) design. Quantification of curcumin and xanthorrhizol are carried out using reversed-phase high-performance liquid chromatography (RP HPLC) equiped by a photodiode array detector. Extraction time 20 minutes, temperature of 50°C, and LS ratio 8 mL/g are found as the optimum condition of extraction yield and quantification of xanthorrhizol. Curcumin 's optimum state, however, is observed at extraction time 12.50 minutes, temperature 30 ° C, and liquid-solid ratio of 10 mL / g. This study suggests that there are favorable potential using method of ultrasound-assisted extraction under specific parameters in the extraction process which is advantageous for advanced research.

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Optimum Conditions and LC-ESI-MS Analysis of Phenolic Rich Extract from Eucalyptus marginata L. under Maceration and Ultrasound-Assisted Extraction Methods Using Response Surface Methodology

Journal of Food Quality ◽

10.1155/2021/5591022 ◽

2021 ◽

Vol 2021 ◽

pp. 1-14

Author(s):

Soumaya Hasni ◽

Ghayth Rigane ◽

Hanene Ghazghazi ◽

Hajer Riguene ◽

Amir Bouallegue ◽

...

Keyword(s):

Response Surface Methodology ◽

Phenolic Compounds ◽

Response Surface ◽

Total Phenolic ◽

Ultrasound Assisted Extraction ◽

Eucalyptus Marginata ◽

Esi Ms ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted

Eucalyptus marginata L. has a significant value in traditional medicine and recently has been shown to possess many pharmacological properties in vitro. The main goal of the present study was to optimize the extraction parameters of phenolic compounds from Eucalyptus marginata L. leaves using the extraction technique assisted by ultrasound in comparison with maceration using response surface methodology as a predicted tool. Therefore, total phenolic and flavonoid contents have been optimized, taking into account four variables: extraction time, temperature, liquid-to-solid ratio, and ethanol concentration. The optimum ultrasound-assisted extraction method for total phenolic and total flavonoid contents was obtained by ensuring the following parameters: t = 49.9 min, T = 74.9°C, liquid-to-solid ratio = 39.5 ml/g, and ethanol = 58.48%. The optimum extract has been subjected to LC-ESI-MS analysis. This technique allowed us to identify ten phenolic compounds: four phenolic acids mainly gallic acid (27.77 ± 0.06 µg/g DW) and protocatechuic acid (37.66 ± 0.04 µg/g DW) and six flavonoid compounds such as quercetrin (150.78 ± 0.02 µg/g DW) and hyperoside (39.19 ± 0.03 µg/g DW). These green and efficient procedures should be a promising option to guide industrial design for the production of phenolic-rich plant extracts.

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Ultrasound-Assisted Extraction Optimization of α-Glucosidase Inhibitors from Ceratophyllum demersum L. and Identification of Phytochemical Profiling by HPLC-QTOF-MS/MS

Molecules ◽

10.3390/molecules25194507 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4507

Author(s):

Zhen Li ◽

Zongcai Tu ◽

Hui Wang ◽

Lu Zhang

Keyword(s):

Chinese Herb ◽

Ceratophyllum Demersum ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Glucosidase Inhibitors ◽

Assisted Extraction ◽

Ic50 Value ◽

Ultrasound Assisted

Ceratophyllum demersum L. (CDL) is a traditional Chinese herb to treat many diseases, but research on its anti-diabetic activity is not available. In this research, the α-glucosidase inhibitory ability and phytochemical constituents of CDL extract were firstly studied. Optimal ultrasound-assisted extraction conditions for α-glucosidase inhibitors (AGIs) were optimized by single factor experiment and response surface methodology (RSM), which was confirmed as 70% methanol, liquid-to-solid ratio of 43 (mL/g), extraction time of 54 min, ultrasonic power of 350 W, and extraction temperature of 40 °C. The lowest IC50 value for α-glucosidase inhibition was 0.15 mg dried material/mL (mg DM/mL), which was much lower than that of acarbose (IC50 value of 0.64 mg DM/mL). In total, 80 compounds including 8 organic acids, 11 phenolic acids, 25 flavonoids, 21 fatty acids, and 15 others were identified or tentatively identified from CDL extract by HPLC-QTOF-MS/MS analysis. The results suggested that CDL could be a potential source of α-glucosidase inhibitors. It can also provide useful phytochemical information for research into other bioactivities.

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Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/1208617 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Xiang-Yue Huang ◽

Hua-Lin Fu ◽

Hua-Qiao Tang ◽

Zhong-Qiong Yin ◽

Wei Zhang ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Bacterial Infections ◽

Maximum Yield ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Arnebia Euchroma ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Time Kill

The objectives of this study were to develop and optimize ultrasound-assisted extraction (UAE) for shikonin from Arnebia euchroma using response surface methodology (RSM) and to evaluate the antimicrobial activity of shikonin. The maximum yield of shikonin was 1.26% under the optimal extraction conditions (ultrasound power, 93 W; time, 87 min; temperature, 39°C; and liquid-solid ratio, 11 : 1). Shikonin showed inhibitory activity against standard strains and clinical isolates to varying extents (MICs ranging from 128 to 1024 μg/mL, MBCs ranging from 256 to 2048 μg/mL), and it was more effective for Gram-positive bacteria as indicated by lower MIC and MBC values. Time-kill curves revealed that antibacterial activity of shikonin exhibited a dose-response relationship. In summary, via this study, we identified ultrasound-assisted RSM as the optimal extraction method for shikonin, which is a potential material for the treatment of bacterial infections.

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Optimization of Ultrasound-Assisted Extraction of Polysaccharides from the Chinese Medicinal Plant Rhodiola Sachalinensis A. BOR

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.448-453.1085 ◽

2013 ◽

Vol 448-453 ◽

pp. 1085-1088

Author(s):

Chuan Jun Zhang ◽

Feng Lin Li

Keyword(s):

Medicinal Plant ◽

Extraction Time ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Rhodiola Sachalinensis ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Chinese Medicinal Plant

Ultrasound-assisted extraction (UAE) of polysaccharides from the Chinese medicinal plantRhodiola sachalinensisA. BOR (PRs) was studied. The effects of four factors (extraction time, ultrasonic power, liquid-solid ratio and extraction temperature) on the extraction yield of PRs were investigated by the single-factor test, and the optimum UAE conditions were investigated by the orthogonal test. The results demonstrated that the optimum UAE conditions for PRs were as followings: extraction time of 30 min, ultrasonic power of 85 W, liquid-solid ratio of 45 ml/g, and extraction temperature of 45°C. The present data might provide a theoretic base for full utilization ofRhodiola sachalinensisA. BOR.

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Optimization of Ultrasound-Assisted Extraction of Flavonoids from Celastrus hindsii Leaves Using Response Surface Methodology and Evaluation of Their Antioxidant and Antitumor Activities

BioMed Research International ◽

10.1155/2020/3497107 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Dinh-Chuong Pham ◽

Hoang-Chinh Nguyen ◽

Thanh-Hang Le Nguyen ◽

Hoang-Linh Ho ◽

Thien-Kim Trinh ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Biological Activities ◽

Radical Scavenging ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Anticancer Activities ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

Celastrus hindsii is a potential source of flavonoids with biological activities. This study aimed to develop an ultrasound-assisted technique for extracting flavonoids from leaves of C. hindsii. Response surface methodology was employed to optimize the extraction conditions for maximizing the total flavonoid content (TFC). A maximum TFC of 23.6 mg QE/g was obtained under the extraction conditions of ultrasonic power of 130 W, extraction temperature of 40°C, extraction time of 29 min, and ethanol concentration of 65%. The flavonoid-rich extracts were then studied for their antioxidant and anticancer activities. The results showed that the C. hindsii leaf extract exhibited potent radical scavenging activities against DPPH (IC50 of 164.85 μg/mL) and ABTS (IC50 of 89.05 μg/mL). The extract also significantly inhibited the growth of 3 cancer cell lines MCF7, A549, and HeLa with the IC50 values of 88.1 μg/mL, 120.4 μg/mL, and 118.4 μg/mL, respectively. Notably, the extract had no cytotoxicity effect on HK2 normal kidney cell line. This study suggests that flavonoid-rich extract is a promising antioxidant and anticancer agent and that ultrasound-assisted extraction is an efficient method for extracting flavonoids from C. hindsii leaves.

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UPLC-ESI-MS/MS Analysis and Evaluation of Antioxidant Activity of Total Flavonoid Extract from Paeonia lactiflora Seed Peel and Optimization by Response Surface Methodology (RSM)

BioMed Research International ◽

10.1155/2021/7304107 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Guiqin Shi ◽

Jiaxin Shen ◽

Tao Wei ◽

Fei Ren ◽

Famou Guo ◽

...

Keyword(s):

Antioxidant Activity ◽

Response Surface Methodology ◽

Response Surface ◽

Ethanol Concentration ◽

Paeonia Lactiflora ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasound Assisted

In this study, the ultrasound-assisted extraction (UAE) of flavonoid from Paeonia lactiflora seed peel was optimized by response surface methodology (RSM). Single-factor experiments and a three-factor three-level Box-Behnken design (BBD) were performed to explore the effects of the following parameters on flavonoid extraction: ethanol concentration ( X 1 ), liquid-solid ratio ( X 2 ), and ultrasonic time ( X 3 ). The results showed that the optimal flavonoid yield (10.9045 mg RE/g) was as follows: ethanol concentration 62.93%, ultrasonic time 64.56 min, and liquid-solid ratio 24.86 mL/g. The optimized extract of P. lactiflora seed shell was further analyzed by UPLC-ESI-MS/MS, and 20 main flavonoids were identified and quantified, among which protocatechuic acid, vanillic acid, 4-hydroxybenzoic acid, and 3,4-dihydroxybenzaldehyde had the highest content. Furthermore, the results of the antioxidant test showed that the P. lactiflora seed peel extract obtained under optimized UAE conditions exhibited good antioxidant activity. The experimental results showed that ultrasound-assisted extraction was a fast, efficient, and simple method for extracting active ingredients from P. lactiflora seed peel, thereby making this byproduct a promising source of compounds in food and healthcare sectors.

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