scholarly journals Characterization of Ciprofloxacin-Bismuth-Loaded Antibacterial Wound Dressing

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5096
Author(s):  
Dorota Kowalczuk ◽  
Małgorzata Miazga-Karska ◽  
Agata Gładysz ◽  
Paweł Warda ◽  
Agnieszka Barańska ◽  
...  
Keyword(s):  
Wound Dressing ◽  
High Performance ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Chromatography Method ◽  
Modification Process ◽  
Bromine Solution ◽  
Before And After ◽  
Liquid Chromatography Method

The research was focused on developing a potentially antibacterial wound dressing made of polyurethane foam and loaded with bismuth-ciprofloxacin (Cip-Bi). The Cip-Bi chemical structure was confirmed by Fourier transform infrared spectroscopic (FTIR) analysis. The sought after antibacterial wound dressing was obtained by modification of the raw dressing with an iodine or bromine solution and subsequently with a Cip-Bi hydrogel. The amount of Cip-Bi loaded into the dressing matrix was determined indirectly on the basis of the differences in Cip-Bi concentrations, before and after the modification process, and the determination was performed with the HPLC (high-performance liquid chromatography) method. The modified dressing was found to have a two-step release of Cip-Bi, a feature helpful in the treatment of locally infected wounds and prevention of secondary bacterial infection. The zone of inhibition test against the selected Gram-positive and Gram-negative bacteria confirmed the antibacterial activity of the Cip-Bi-modified dressing. Preliminary tests conducted so far have been indicative of the Cip-Bi dressing’s relatively high activity against the tested organisms.

Thiamine levels in Indonesian children with congenital heart diseases undergoing surgery using cardiopulmonary bypass machine

2021 ◽  
pp. 021849232110241
Author(s):  
Eva M Marwali ◽  
Putri Caesa ◽  
Yoel Purnama ◽  
Muhammad Rayhan ◽  
Novik Budiwardhana ◽  
...  
Keyword(s):  
Cardiopulmonary Bypass ◽  
High Performance ◽  
Congenital Heart ◽  
Heart Diseases ◽  
Cross Sectional Study ◽  
Congenital Heart Diseases ◽  
Chromatography Method ◽  
Cross Sectional ◽  
Before And After ◽  
Liquid Chromatography Method

Background This study evaluated thiamine levels in Indonesian children with congenital heart diseases before and after cardiopulmonary bypass and their relationship with clinical and surgical outcomes. Method A prospective, single center cross-sectional study was conducted to evaluate thiamine levels in 25 children undergoing congenital heart diseases surgery with cardiopulmonary bypass procedure. Thiamine levels were quantified using a high-performance liquid chromatography method. Result Preoperative thiamine deficiency was observed in one subject. Thiamine levels did not differ statistically between nutritional status and clinical outcomes categories. There were no significant changes in thiamine levels before and after cardiopulmonary bypass (median pre versus post (P25–75): 50 ng/mL (59.00–116.00) and 83.00 ng/mL (70.00–101.00), p = 0.84), although a significant reduction in thiamine levels were observed with longer cardiopulmonary bypass duration ( p = 0.017, R = −0.472). Conclusion Thiamine levels were not significantly impacted by cardiac surgery except in patients undergoing extremely long cardiopulmonary bypass duration. However, clinical outcome was not affected by thiamine levels.

scholarly journals The Effect of Dicarboxymethyl Cellulose on the Prevention of Protein Haze Formation on White Wine

Beverages ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 57
Author(s):  
Diana Gago ◽  
Ricardo Chagas ◽  
Luísa M. Ferreira
Keyword(s):  
High Performance ◽  
Heat Stability ◽  
White Wine ◽  
Protein Quantification ◽  
Chromatography Method ◽  
White Wines ◽  
Heat Stable ◽  
Before And After ◽  
Haze Formation ◽  
Liquid Chromatography Method

Wine clarity is a critical aspect in the commercialization of white wines. The formation of wine haze can be attributed to the aggregation and precipitation of heat-unstable wine proteins. Bentonite fining is the commonly used method in winemaking for protein removal, but it is responsible for loss of wine volume and quality. Dicarboxymethyl cellulose (DCMC) was developed as a potential alternative to bentonite. Water-insoluble DCMC was prepared via catalyzed heterogeneous etherification using sodium chloromalonate and potassium iodide. White wine fining trials were benchmarked with different dosages of DCMC against a bentonite. A high-performance liquid chromatography method was optimized for protein quantification. The samples underwent heat stability tests to evaluate wine turbidity before and after fining. Results show that DCMC successfully reduced the wine protein content and turbidity. DCMC produced heat-stable wines with dosages higher than 0.25 g/L. The innovative application of DCMC in the wine sector shows potential due to its ability to stabilize white wines while overcoming problems associated with bentonite, such as lees production and loss of wine, contributing to a more sustainable process.

scholarly journals A VALIDATED STABILITY-INDICATING REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DACLATASVIR, IDENTIFICATION AND CHARACTERIZATION OF DEGRADATION PRODUCTS USING LC-ESI-QTOF-MS

2019 ◽  
pp. 302-308
Author(s):  
SNEHAL V WARGHADE ◽  
KAILAS G BOTHARA
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Degradation Products ◽  
Antiviral Drug ◽  
Array Detector ◽  
Forced Degradation ◽  
Chromatography Method ◽  
Liquid Chromatography Method ◽  
The Stability

Objective: The objective of this study was to report the stability of antiviral drug, daclatasvir (DCV) based on the information obtained from forced degradation studies and characterization of degradation products (DPs) by tandem mass spectrometry (MS/MS) analysis. Methods: Chromatographic separation was achieved on Shimadzu liquid chromatography (LC) 20 AD high-performance LC system with photodiode array detector having Kromasil C18 (250 mm×4.6 mm×5 μm) with isocratic elution of a mobile phase composed of ammonium acetate buffer (pH 4.5) and acetonitrile in a ratio of 50:50 at 315 nm. The drug was subjected to forced hydrolytic, oxidative, photolytic, and thermal stress in accordance with the ICH guideline Q1A (R2). The drug showed degradation under acidic and basic hydrolytic conditions by forming two DPs. The DPs were characterized using LC– MS/MS studies and the pathways of fragmentation are proposed. Validation of the developed method was carried out in accordance with ICH guidelines. Results: Two DPs were identified, DP-1 as (S)-1-((S)-2-(5-(4’-(2-((S)-1-((S)-2-((methoxycarbonyl)amino)-3-methylbutanoyl)pyrrolidin-2-yl)-1H-imidazol-5-yl)-[1,1’-biphenyl]-4-yl)-1H-imidazol-2-yl)pyrrolidin-1-yl)-3-methyl-1-oxobutan-2-aminium and DP-2 as (S)-2-(5-(4’-(2-((S)-1-((S)-2- ((methoxycarbonyl)amino)-3-methylbutanoyl)pyrrolidin-2-yl)-1H-imidazol-5-yl)-[1,1’-biphenyl]-4-yl)-1H-imidazol-2-yl)pyrrolidin-1-ium. Conclusion: The method proved to be simple, accurate, precise, specific, robust, and less time consuming and can be applied for the determination of DCV in bulk and marketed formulation.

scholarly journals Fast Quenching the Burst of Host Salicylic Acid Is Common in Early Strawberry/Colletotrichum fructicola Interaction

2019 ◽  
Vol 109 (4) ◽  
pp. 531-541
Author(s):  
Chengyong He ◽  
Ke Duan ◽  
Liqing Zhang ◽  
Ling Zhang ◽  
Lili Song ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
High Performance ◽  
De Novo ◽  
Chromatography Method ◽  
Colletotrichum Fructicola ◽  
Genes Encoding ◽  
Localization Analysis ◽  
Liquid Chromatography Method ◽  
Fast Quenching

The fungus Colletotrichum fructicola (a species of C. gloeosporioides complex) causes devastating anthracnose in strawberry. Like other species of the genus Colletotrichum, it uses a composite strategy including both the biotrophic and necrotrophic processes for pathogenesis. Host-derived hormones are central regulators of immunity, among which salicylic acid (SA) is the core defense one against biotrophic and hemibiotrophic pathogens. However, the manner and timing of pathogen manipulation of SA are largely elusive in strawberry. To achieve better understanding of the early challenges that SA-mediated defense experiences during strawberry/C. fructicola interaction, dynamic changes of SA levels were followed through the high-performance liquid chromatography method. A very early burst of free SA at 1 h postinoculation (hpi) followed by a fast quenching during the next 12 h was noticed, although rhythm variations were present in two hosts. Transcriptional characterization of genes related to SA pathway in two varieties on C. fructicola inoculation revealed that these genes were differentially expressed, although they were all induced at different time points. At the same time, three types of genes encoding homologous effectors interfering with SA accumulation were found to be first inhibited but sequentially activated during the first 24 hpi. Furthermore, subcellular localization analysis suggests that CfShy1 is a weapon of C. fructicola for strawberry invasion. Based on these results, we propose that the infection strategy that C. fructicola utilizes on strawberry is specialized, which is implemented through the optimized expression of a specific set of effector genes. Transcriptional characterization of host genes supports that de novo SA biosynthesis and the free SA release from methyl salicylate might contribute equally to the intricate control of SA homeostasis in strawberry. C. fructicola manipulation of SA-dependent resistance in strawberry might be closely related to multihormonal interplay among SA, jasmonic acid, abscisic acid, and cytokinin.

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