scholarly journals Hepatoprotective Effect of Supercritical Carbon Dioxide Extracted Dabai Pulp Oil and Its Defatted Pulp

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 671
Author(s):  
Noor Atiqah Aizan Abdul Kadir ◽  
Azrina Azlan ◽  
Faridah Abas ◽  
Intan Safinar Ismail
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Functional Foods ◽  
Density Lipoprotein ◽  
Histological Section ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Hepatoprotective Effects ◽  
Supercritical Carbon

All food scientists must utilize plants for their application as functional foods to reduce hypercholesterolemia incidence through diet. Canarium odontophyllum (dabai) is a novel source for new healthy oil and functional foods. In this work, we evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO2) extracted dabai pulp oil (DPO) and defatted dabai pulp (DDP) against hypercholesterolemia elicited by a high-cholesterol diet in rats. Our results show that DPO and DDP supplementation exerted beneficial hypocholesterolemic effects against the high-cholesterol diet-fed rat. Nevertheless, supplementation with DDP revealed superior total cholesterol, low-density lipoprotein, and HMG-CoA reductase lowering efficacy (p < 0.05). Supplementation of either DPO or DDP did not significantly affect AST and ALT levels than normal rats (p > 0.05). Therefore, DDP and DPO are considered as having no toxicological significance. The histological section of rats treated with DPO and DDP showed improved steatosis in hepatocytes. HPLC analysis revealed that DPO and DDP contained syringic acid, which plays an important role in the beneficial effect. In conclusion, our results support the hypocholesterolemic and hepatoprotective effects of DPO and DDP in the hypercholesterolemic rats model.

Emodin alleviated hyperlipidemia and hepatic lipid metabolism in zebrafish larvae fed a high-cholesterol diet via AMPK/SREBP-2/PCSK9/LDLR signaling pathway

2021 ◽  
Author(s):  
Linfeng He ◽  
Cheng Wang ◽  
Yafang Zhang ◽  
Chaocheng Guo ◽  
Yan Wan ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Cholesterol Diet ◽  
Low Density ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cholesterol Accumulation ◽  
Hepatic Lipid ◽  
Zebrafish Larvae

Abstract BackgroundEmodin (EM) is one of bioactive components extracted from Rheum palmatum L. (Dahuang), which possesses numerous pharmacological activities including hypolipidemic effect. However, the potential action of EM on hyperlipidemia (HLP) remains unclear. Here, the theraputic effect of EM against HLP were investigated.MethodsIn this study, the hypolipidemic properties of EM were evaluated using high-cholesterol diet (HCD)-stimulated zebrafish larvae model. The body weight, body length and body mass index (BMI) was measured. The total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) as well as the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected by corresponding assay kits. Tg (flil: eGFP) zebrafish were utilized to observe vascular cholesterol accumulation and Tg (mpx: eGFP) zebrafish to visualize and quantify neutrophil inflammation. The hepatic lipid deposition and hepatic histopathology were analyzed by Oil red O staining and H&E staining, respectively. Finally, the underlying mechanism of EM were investigated using real-time quantitative PCR (RT-qPCR) analysis to assess the gene levels of adenosine monophosphate-activated protein kinase alpha (AMPKα), sterol regulatory element binding protein 2 (SREBP-2), proprotein convertase subtilisin kexin 9 (PCSK9), low-density lipoprotein receptor (LDLR), 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR), adenosine triphosphate binding cassette transporter A1 (ABCA1) and adenosine triphosphate binding cassette transporter G1 (ABCG1).ResultsOur data indicated that EM reduced obesity of zebrafish as evidenced by the decrease in body weight, body length and BMI. EM significantly reduced TC, TG, and LDL-C, and increased HDL-C contents. Moreover, it displayed a prominent inhibitory effect on blood cholesterol accumulation, hepatic lipid accumulation, and neutrophil inflammation in vascular site. Additionally, EM improved the liver function through decreasing ALT and AST levels of zebrafish with HCD-induced hepatosteatosis. Further investigation showed that EM treatment attenuated lipid accumulation via upregulating the expression of AMPKα, LDLR, ABCA1 and ABCG1, and downregulating the expression of SREBP-2, PCSK9 and HMGCR.ConclusionTo conclude, EM alleviated lipid metabolism disorder symptoms caused by HCD via modulating AMPK/SREBP-2/PCSK9/LDLR pathway in larvae, suggesting that EM may be developed into hypolipidmic agent for treating lipid metabolism related diseases.

scholarly journals Evaluation of the Hypocholesterolemic Effect and Phytochemical Screening of the Hydroethanolic Extract of Crataegus Aronia from Jordan

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Entisar K. Al-Hallaq ◽  
Fatma U. Afifi ◽  
Shtaywy S. Abdalla
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Cholesterol Diet ◽  
Hypocholesterolemic Effect ◽  
Cholesterol Diet ◽  
Phytochemical Screening ◽  
High Cholesterol ◽  
Chemical Screening ◽  
Hydroethanolic Extract ◽  
First Time

Chemical screening of the leaves and flowers of Crataegus aronia resulted in the isolation of hyperoside, quercetin, rutin and β-sitosterol for the first time from this plant. The effects of the hydroethanolic extract of C. aronia (CAHE) on hypercholesterolemic rats were investigated. The rats, treated orally for four weeks with 400 mg/kg/day CAHE, exhibited significant decreases in serum total cholesterol (TC) and low-density lipoprotein (LDL). The results were compared with those obtained after oral administration of atorvastatin (10 mg/kg/day). Furthermore, 10-week daily co-administration of a high cholesterol diet and CAHE (200 mg/kg/day) prevented the increase in TC and LDL. These observations indicate that CAHE has a hypocholesterolemic effect.

In vitro and in vivo cholesterol lowering ability of Lactobacillus pentosus KF923750

2017 ◽  
Vol 8 (2) ◽  
pp. 271-280 ◽  
Author(s):  
F. Bendali ◽  
K. Kerdouche ◽  
S. Hamma-Faradji ◽  
D. Drider
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Lactobacillus Pentosus ◽  
Survival Percentage

Lactobacillus pentosus KF923750 was characterised for probiotic related properties and then characterised for cholesterol uptake in vitro as well as in vivo using rabbits fed a high-cholesterol diet. The survival percentage of L. pentosus KF923750 was 100% at pH 3, 52.18% at pH 2 and 36.21% at pH 2 plus pepsin. Similarly, this strain appeared resistant to bile (0.1% [98.42%], 0.3% [88.52%], 0.5% [75.60%] and 1% [71.15%]), after 4 h exposure. Moreover, L. pentosus KF923750 controlled growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 through the production of a bacteriocin-like inhibitory substance and anti-adhesive capabilities. L. pentosus KF923750 was non-cytotoxic to eukaryotic cells but sensitive to some antibiotics. Compared with rabbits fed a high-cholesterol diet but without L. pentosus KF923750 supplementation, the plasma total cholesterol, low-density lipoprotein cholesterol and triglycerides levels were significantly decreased in L. pentosus KF923750-fed rabbits by 11.54, 16.00 and 18.00%, respectively, with no significant change in high-density lipoprotein cholesterol levels. The histological sections of livers revealed lesions in all the rabbits that were fed a high-cholesterol diet, but these were less pronounced in rabbits ingesting L. pentosus KF923750. This study highlights the potential of lactobacilli, such as L. pentosus KF923750, in the treatment or prevention of hypercholesterolemia.

scholarly journals Antidyslipidaemic and cardioprotective effects of turmeric (Curcuma longa) in rat fed a high cholesterol diet

2020 ◽  
Vol 10 (1-s) ◽  
pp. 178-181
Author(s):  
Ikenna Kingsley Uchendu ◽  
Ifeoma Blessing Ekeigwe ◽  
Ebuka Bitrus Nnedu
Keyword(s):  
Biochemical Markers ◽  
Curcuma Longa ◽  
Density Lipoprotein ◽  
Negative Control ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Muscle Fibres ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Cardioprotective Effects

Objective: The aim of this study is to evaluate the antidyslipidaemic and cardioprotective effects of aqueous extract of Curcuma longa (AECL) in rats fed a high cholesterol diet (HCD). Methods: Twenty (20) rats were randomly grouped into four groups: A-D of five animals per group. Groups A-C received HCD (2000mg/kg, oral) and carbimazole (60mg/kg, oral) daily for eight weeks. Group A served as negative control. Group B (positive control) was treated with atorvastatin (20mg/kg), while group C served as treatment group and received AECL (400mg/kg) daily for eight weeks. Group D served as normal control and received no treatment. After the administration, biochemical markers of Lipid profiles (total cholesterol (TC), triglycerides (TG) and high density lipoprotein cholesterol (HDL-C)) were assayed using standard methods. The hearts of the rats were harvested for histopathological studies. Results: AECL significantly induced hypolipidaemia and stabilized lipid biochemical markers (p<0.05 or p<0.01); and protected the cardiac muscle fibres from injuries. Conclusion: Tumeric (Curcuma longa) has antidyslipidaemic and cardioprotective effects. Keywords: antidyslipidaemia, cardioprotection, Curcuma longa, ethnopharmacology, hypercholesterolaemia, Medicinal foods, cardiomyopathy

scholarly journals EFFECT OF SODIUM ALGINATE ON PREVENTION OF HYPERCHOLESTEROLEMIA AND ATHEROSCLEROSIS IN RATS

2018 ◽  
Vol 11 (6) ◽  
pp. 242 ◽  
Author(s):  
Yulis Kartika ◽  
Hakim Bangun ◽  
Rosidah Rosidah
Keyword(s):  
Body Weight ◽  
Sodium Alginate ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Foam Cells ◽  
Diet Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Regular Diet

Objective: The present study is to determine the effect of liquid of sodium alginate and powder on the prevention of raising cholesterol levels and atherosclerosis in rats (preventive effect).Method: Experimental animals were divided into prevention groups. Preventive groups used were 24 normal rats and then divided into four groups, each group consisted of 6 animals: Group 1 (fed a regular diet), Group 2 (fed high cholesterol diet), Group 3 (fed high cholesterol diet and alginate liquid 2% diet), and Group 4 (fed high cholesterol and alginate powder). Rats are given with cholesterol food, alginate liquid, alginate powder a dose of 1 ml/100 g body weight once a day. Parameters measured were cholesterol level, low-density lipoprotein (LDL) levels, high-density lipoprotein (HDL) levels, triglyceride (TG) level, body weight every week, and histopathologically of the aorta for foam cells examined at the end of the experiment. Analysis of data was performed using one-way ANOVA.Results: The lipid profiles in the preventive group showed alginate liquid and powder which have the effect of preventing increase total cholesterol, LDL, TG, number of foam cells, and decrease HDL. Diet using alginate powder better than alginate liquid, but the effect was not different significantly (p<0.05) so do the regular diet, but higher than given with high cholesterol diet significantly (p>0.05).Conclusion: This study showed that the alginate liquid and alginate powder have an anti-hypercholesterolemia property which has been proved by preventing increase LDL, TG, and increased HDL levels.

Hypocholesterolemic effect of camel milk on rats fed a high-cholesterol diet

2018 ◽  
Vol 30 (4) ◽  
pp. 288 ◽  
Author(s):  
Mohammed A. Yahya ◽  
Omar A. Alhaj ◽  
Abdullrahman S. AL-Khalifah ◽  
Ahmad T. Almnaizel
Keyword(s):  
Wistar Rats ◽  
Skim Milk ◽  
Camel Milk ◽  
High Cholesterol Diet ◽  
Hypocholesterolemic Effect ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Tissue Degeneration ◽  
Histopathological Evaluation ◽  
Hepatoprotective Effects

The effects of fermented skim milk versus unfermented skim milk of camel on the levels of cholesterol in blood were investigated in rats. Levels of serum cholesterol and LDL-C/ HDL-C ratio were decreased significantly in Wistar rats that fed with a cholesterol-enriched diet and administered fermented skim camel milk compared with rats administered unfermented milk (P˂0.05). Furthermore, histopathological evaluation showed that liver tissue degeneration, apoptosis/necrosis, inflammation, and fatty changes (steatosis and fibrosis) decreased significantly at (P˂0.05) in the rats that fed with fermented skim camel milk compared to the rats which fed unfermented skim camel milk. Based on these results it can suggest that fermented skim camel milk might reduce the risk of hypercholesterolemia development in rats. The hypocholesterolemic and hepatoprotective effects of fermented skim camel milk were evident.

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