Variability of the Chemical Composition of the Essential Oil from the Amazonian Ishpingo Species (Ocotea quixos)

Ocotea quixos (Lam.) Kosterm. is an aromatic tree native to Ecuador, whose leaves are used to prepare aromatic beverages to which different health benefits are attributed. In this study, Ocotea quixos leaves were collected in the Amazon region in different environmental conditions and subjected to hydrodistillation to isolate the essential oil. The collection variables used were type of soil, amount of shade, and height; in addition, the presence of twig and leaf age and moisture were used as variables. Chemical composition was analyzed by means of gas chromatography equipped with a flame ionization detector and gas chromatography coupled to mass spectrometry. A wide variety of chemical compositions were detected in the samples. In total, forty-seven compounds were identified, which represented between 97.17% and 99.89% of the total composition. The constituents were mainly grouped into aliphatic sesquiterpene hydrocarbons (33.03–55.89%), other compounds (8.94–47.83%), and oxygenated monoterpenes (1.97–39.66%). The main constituents were found to be (E)-cinnamyl acetate (5.96–41.65%), (E)-methyl cinnamate (0.38–37.91%), and trans-caryophyllene (8.77–37.02%). The statistical analysis suggested the existence of two essential oil chemotypes and a direct correlation between environmental conditions and chemical composition of the essential oils.

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Chemical Composition of Essential Oils from the Leaves of Syzygium Bullockii and Syzygium Tsoongii in Ke Go Nature Reserve, Ha Tinh Province

VNU Journal of Science Natural Sciences and Technology ◽

10.25073/2588-1140/vnunst.4999 ◽

2021 ◽

Vol 37 (2) ◽

Author(s):

Tran Hau Khanh ◽

Pham Hong Ban ◽

Tran Minh Hoi

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Nature Reserve ◽

Chemical Compositions ◽

Flame Ionization ◽

Leaf Oil ◽

Total Composition ◽

First Time

Chemical composition of essential oils obtained from the leaf of Syzygium bullockii and Syzygium tsoongii were reported, corresponding oil content 0,36% and 0,27%. The analysis was performed by means of gas chromatography-flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Twenty-seven components were identified in leaf oil of Syzygium bullockii, which represented about 76,75% of the total composition of the oil and six components were not identified, which represented 15,72%. The major constituents of the essential oil were (E)-caryophyllene (49,65%), spathulenol (4,29%), caryophyllene oxide (4,14%), bicyclogermacrene (3,35%), 2-tridecanone (3,25%). Fourty-six components were identified in leaf oil of Syzygium tsoongii, which presented about 99.71% of the total composition of the oil. The major constituents of the essential oil were E-caryophyllene (23,40%), bicyclogermacrene (21,23%), (Z)-β-ocimene (10,61%), α-humulene (6,33%), (E)-β-ocimene (4,99%). For the first time, the chemical compositions of the essential oils of Syzygium bullockii and Syzygium tsoongii from Vietnam were being reported.

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Chemical Composition of Vernonia albicans Essential Oil from India

Natural Product Communications ◽

10.1177/1934578x1400900728 ◽

2014 ◽

Vol 9 (7) ◽

pp. 1934578X1400900

Author(s):

Rajesh K. Joshi

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Flame Ionization Detector ◽

Ionization Detector ◽

Flame Ionization ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons ◽

Total Oil

The chemical composition of the hydro-distilled essential oil obtained from the flowering aerial parts of Vernonia albicans DC. (Asteraceae) was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with a mass spectrometry (GC/MS). Thirty-nine compounds have been identified, representing 97.5% of the total oil. The major constituents were β-caryophyllene (34.3%), γ-amorphene (19.5%), 9-epi-β-caryophyllene (6.9%), and α-pinene (6.9%). The oil was found to be rich in sesquiterpene hydrocarbons (73.9%).

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Chemical Composition of the Essential Oil of Lepidagathis Fasciculata from Bondla Forest of Goa, India

Natural Product Communications ◽

10.1177/1934578x1300800834 ◽

2013 ◽

Vol 8 (8) ◽

pp. 1934578X1300800

Author(s):

Rajesh K. Joshi

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Ionization Detector ◽

Flame Ionization ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D ◽

Total Oil

The hydro-distilled essential oil obtained from the flowering aerial parts of Lepidagathis fasciculata Nees was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC/MS). A total of 38 compounds have been identified, representing 91.2% of the total oil. The major constituents were δ-cadinene (14.4 %), γ-curcumene (9.8%), sandaracopimarinal (6.6%), germacrene D-4-ol (6.1%), cembrene (5.0%), β-calacorene (3.6%), ar-curcumene (3.3%), trans–4,10-epoxy-amorphane (3.2%), abietatriene (2.9%), and α-cubebene (2.8%). The oil was rich in sesquiterpene hydrocarbons (43.8%).

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GC/MS Analysis of the Essential Oil of Leucas indica from India

Natural Product Communications ◽

10.1177/1934578x1400901119 ◽

2014 ◽

Vol 9 (11) ◽

pp. 1934578X1400901 ◽

Cited By ~ 4

Author(s):

Rajesh K. Joshi

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Ionization Detector ◽

Flame Ionization ◽

Aerial Parts ◽

Sesquiterpene Hydrocarbons ◽

Total Oil ◽

First Time

The aim of the present study was to investigate and identify the essential oil constituents of Leucas indica (L.) R.Br. (Lamiaceae). The chemical composition of the hydro-distilled essential oil was obtained from the flowering aerial parts of L. indica for the first time. The oil was analyzed by gas chromatography equipped with flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC/MS). Fifty-six compounds were identified, representing 99.1% of the total oil. The main constituents were β-caryophyllene (51.1%) and α-caryophyllene (10.2%). The oil was found to be rich in sesquiterpene hydrocarbons (71.8%).

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Variability of the Chemical Composition and Bioactivity between the Essential Oils Isolated from Male and Female Specimens of Hedyosmum racemosum (Ruiz & Pav.) G. Don

Molecules ◽

10.3390/molecules26154613 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4613

Author(s):

Eduardo Valarezo ◽

Vladimir Morocho ◽

Luis Cartuche ◽

Fernanda Chamba-Granda ◽

Magdaly Correa-Conza ◽

...

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Native Species ◽

Strong Activity ◽

Male And Female ◽

Flame Ionization ◽

Scavenging Capacity ◽

Stomach Pain ◽

Total Composition

Hedyosmum racemosum (Ruiz & Pav.) G. is a native species of Ecuador used in traditional medicine for treatment of rheumatism, bronchitis, cold, cough, asthma, bone pain, and stomach pain. In this study, fresh H. racemosum leaves of male and female specimens were collected and subjected to hydrodistillation for the extraction of the essential oil. The chemical composition of male and female essential oil was determined by gas chromatography–gas chromatography equipped with a flame ionization detector and coupled to a mass spectrometer using a non-polar and a polar chromatographic column. The antibacterial activity was assayed against five Gram-positive and two Gram-negative bacteria, and two dermatophytes fungi. The scavenging radical properties of the essential oil were evaluated by DPPH and ABTS assays. The chemical analysis allowed us to identify forty-three compounds that represent more than 98% of the total composition. In the non-polar and polar column, α-phellandrene was the principal constituent in male (28.24 and 25.90%) and female (26.47 and 23.90%) essential oil. Other main compounds were methyl chavicol, germacrene D, methyl eugenol, and α-pinene. Female essential oil presented a strong activity against Klebsiella pneumoniae (ATCC 9997) with an minimum inhibitory concentration (MIC) of 500 μg/mL and a scavenging capacity SC50 of 800 µg/mL.

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Chemical Constituents of the Essential Oil from Ecuadorian Endemic Species Croton ferrugineus and Its Antimicrobial, Antioxidant and α-Glucosidase Inhibitory Activity

Molecules ◽

10.3390/molecules26154608 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4608

Author(s):

Eduardo Valarezo ◽

Génesis Gaona-Granda ◽

Vladimir Morocho ◽

Luis Cartuche ◽

James Calva ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Endemic Species ◽

Chemical Constituents ◽

Radical Scavenging ◽

Moderate Activity ◽

Gram Positive Bacteria ◽

Flame Ionization ◽

Total Composition ◽

Radical Scavenging Properties

Croton ferrugineus Kunth is an endemic species of Ecuador used in traditional medicine both for wound healing and as an antiseptic. In this study, fresh Croton ferrugineus leaves were collected and subjected to hydrodistillation for extraction of the essential oil. The chemical composition of the essential oil was determined by gas chromatography equipped with a flame ionization detector and gas chromatography coupled to a mass spectrometer using a non-polar and a polar chromatographic column. The antibacterial activity was assayed against three Gram-positive bacteria, one Gram-negative bacterium and one dermatophyte fungus. The radical scavenging properties of the essential oil was evaluated by means of DPPH and ABTS assays. The chemical analysis allowed us to identify thirty-five compounds representing more than 99.95% of the total composition. Aliphatic sesquiterpene hydrocarbon trans-caryophyllene was the main constituent with 20.47 ± 1.25%. Other main compounds were myrcene (11.47 ± 1.56%), β-phellandrene (10.55 ± 0.02%), germacrene D (7.60 ± 0.60%), and α-humulene (5.49 ± 0.38%). The essential oil from Croton ferrugineus presented moderate activity against Candida albicans (ATCC 10231) with an MIC of 1000 μg/mL, a scavenging capacity SC50 of 901 ± 20 µg/mL with the ABTS method, and very strong antiglucosidase activity with an IC50 of 146 ± 20 µg/mL.

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Chemical Composition and Antimicrobial Activities of the Essential Oil From the Leaves of Pterocephalus hookeri

Natural Product Communications ◽

10.1177/1934578x20981239 ◽

2020 ◽

Vol 15 (12) ◽

pp. 1934578X2098123

Author(s):

Peng-fei Yang ◽

Hui Lu ◽

Qiong-bo Wang ◽

Zhi-wei Zhao ◽

Qiang Liu ◽

...

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Chemical Constituents ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Flame Ionization ◽

And Staphylococcus Aureus ◽

Antimicrobial Assay ◽

Detailed Chemical

Detailed chemical constituents of essential oil from the Pterocephalus hookeri leaves and its antimicrobial activities were investigated in this study. The essential oil, obtained by hydrodistillation, was characterized by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry analyses. Among the 90 identified compounds, hexadecanoic acid (21.27%), phytol (8.03%), furfural (7.08%), oleic acid (5.25%), and phytone (4.56%) were the major components. In the antimicrobial assay, the essential oil showed strong inhibitory activities against Escherichia coli, Candida albicans, and Staphylococcus aureus with minimum inhibitory concentration values of 31.3, 62.5, and 125 µg/mL, respectively. To our knowledge, this is the first report concerning chemical composition and antimicrobial activities of the essential oil from Pterocephalus hookeri.

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Soil and Leaf Nutrients Drivers on the Chemical Composition of the Essential Oil of Siparuna muricata (Ruiz & Pav.) A. DC. from Ecuador

Molecules ◽

10.3390/molecules26102949 ◽

2021 ◽

Vol 26 (10) ◽

pp. 2949

Author(s):

Juan I. Burneo ◽

Ángel Benítez ◽

James Calva ◽

Pablo Velastegui ◽

Vladimir Morocho

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Secondary Metabolites ◽

Essential Oil ◽

Chemical Elements ◽

Climatic Conditions ◽

Gas Chromatography Mass Spectrometry ◽

Chemical Compositions ◽

Leaf Nutrients ◽

First Time

Chemical compositions of plants are affected by the initial nutrient contents in the soil and climatic conditions; thus, we analyzed for the first time the effects of soil and leaf nutrients on the compositions of the essential oils (EOs) of Siparuna muricata in four different localities in Ecuador. EOs were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) and a gas chromatography/flame ionization detector (GC/FID). Enantiomeric distribution by GC/MS was determined, modifying the enantiomeric separation of β-pinene, limonene, δ-elemene, β-bourbonene, cis-cadina-1 (6), 4-diene and atractylone. A total of 44 compounds were identified. The most representative for L1 were guaiol, atractylone and 4-diene; for L2, cis-cadina-1(6),4-diene and myrcene; for L3, atractylone, myrcene and germacrene B; and finally, L4 germacrene B, myrcene and cis-cadina-1(6),4-diene. Correlations between soil- leaf chemical elements such as Al, Ca, Fe, Mg, Mn, N and Si in the different localities were significant with chemical composition of the essential oil of Siparuna muricata; however, correlations between soil and leaf K, P, and Na were not significant. Cluster and NMDS analysis showed high dissimilarity values of secondary metabolites between four localities related with changes in soil- leaf nutrients. Thus, the SIMPER routine revealed that not all secondary metabolites contribute equally to establishing the differences in the four localities, and the largest contributions are due to differences in guaiol, cis-cadina-1(6),4-diene, atractylone and germacrene. Our investigation showed for the first time the influences of altitude and soil- leaf chemical elements in the chemical composition of the EOs of S. muricata.

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Chemical composition and insecticidal activity of Garcinia gardneriana (Planchon & Triana) Zappi (Clusiaceae) essential oil

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas ◽

10.37360/blacpma.21.20.5.37 ◽

2021 ◽

Vol 20 (5) ◽

pp. 503-514

Author(s):

Carla Maria Mariano Fernandez ◽

◽

Fabiana Brusco Lorenzetti ◽

Sirlene Adriana Kleinubing ◽

Joao Paulo Pinguello de Andrade ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Chemical Analysis ◽

Essential Oil ◽

Immersion Test ◽

Acaricidal Activity ◽

Gas Chromatography Mass Spectrometry ◽

Larvicidal Effect ◽

Sesquiterpene Hydrocarbons

The present study aimed to analyze the chemical composition of the essential oil from Garcinia gardneriana (Planchon & Triana) Zappi leaves and fruits, and to determine its acaricidal activity on Rhipicephalus microplus by larval packet test and larvicidal action on Aedes aegypti by larval immersion test. The chemical analysis of the essential oil by gas chromatography-mass spectrometry identified sesquiterpene hydrocarbons and oxygenated sesquiterpenes in bacupari leaves and fruits, and α-cedrene, α-chamigrene, α-trans-bergamotene, and β-curcumene as major compounds. Essential oil from leaves of G. gardneriana presented acaricidal activity on R. microplus (LC50 = 4.8 mg/mL; LC99 = 10.8 mg/mL) and larvicidal effect on A. aegypti (LC50 = 5.4 mg/mL; LC99 = 11.6 mg/mL), whereas essential oil from the fruits of G. gardneriana showed LC50 = 4.6 mg/mL and LC99 = 8.9 mg/mL against R. microplus and LC50 = 6.4 mg/mL and LC99 = 13.9 mg/mL against A. aegypti. These results thus demonstrate the potential acaricidal and larvicidal activity of essential oil of G. gardneriana, offering new perspectives for the realization of bioassays from this essential oil.

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Chemical composition of the essential oil of Psidium guajava leaves and its toxicity against Sclerotinia sclerotiorum

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n2p865 ◽

2018 ◽

Vol 39 (2) ◽

pp. 865

Author(s):

Elizabeth Aparecida Josefi Silva ◽

Vanessa Paula da Silva ◽

Cassia Cristina Fernandes Alves ◽

José Milton Alves ◽

Edson Luiz Souchie ◽

...

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Sclerotinia Sclerotiorum ◽

Psidium Guajava ◽

Gas Chromatography Mass Spectrometry ◽

Flame Ionization ◽

Randomized Design ◽

Dry Seasons ◽

Guava Leaves

Globally, the fungus Sclerotinia sclerotiorum is considered as one of the most important phytopathogens, since it affects the production of several economically important crops. Further, it is difficult to control, thus increasing the use of pesticides. Therefore, the search for new substances, especially those extracted from plants, has received special attention to control this plant pathogen. The objective of the present study was to evaluate the yield and chemical composition of the essential oil extracted from the fresh leaves of guava collected during the rainy and dry seasons, and also to evaluate its toxicity against S. sclerotiorum. The experiment was a completely randomized design with five treatments and six replicates. The essential oil was extracted by hydrodistillation of the leaves and analyzed by gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. The main compounds identified in the essential oil were trans-caryophyllene and ?-humulene. The essential oil at a concentration of 300 ?L exhibited 90% inhibition of the mycelial growth of S. sclerotiorum. This confirmed the antifungal potential of the essential oil of the guava leaves during both the sampling seasons.

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