Ion Chromatography with Pulsed Amperometric Detection for Determining Cyanide in Urine and Meconium Samples

The parents’ addictions and eating habits have a significant influence on the child’s growth. The first stool of a newborn baby provides a large amount of information about xenobiotics transmitted by the mother’s body. The analytical technique used in the study is ion chromatography with pulsed amperometric detection (IC-PAD). The biological samples, which were obtained from women staying in a maternity ward and their partners, revealed cyanide concentrations in urine samples spanning 1.30–25.3 μg L−1. Meanwhile, the results of the meconium samples were in the range of 1.54 μg L−1 to 24.9 μg L−1. Under the optimized chromatographic conditions, the IC-PAD system exhibited satisfactory repeatability (R < 3%, n = 3) and good linearity in the range of 1–100 μg L−1. Thus, it proved to be an effective tool for monitoring trace cyanide concentration in a series of human body fluid matrices, including meconium. Based on the literature review, this is the first application of the IC-PAD analytical technique for the determination of cyanide ions in meconium samples.

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Determination of biogenic amines in fresh and processed meat by ion chromatography and integrated pulsed amperometric detection on Au electrode

Food Chemistry ◽

10.1016/j.foodchem.2007.04.071 ◽

2007 ◽

Vol 105 (4) ◽

pp. 1652-1658 ◽

Cited By ~ 50

Author(s):

Gabriella Favaro ◽

Paolo Pastore ◽

Giovanna Saccani ◽

Silvano Cavalli

Keyword(s):

Biogenic Amines ◽

Ion Chromatography ◽

Amperometric Detection ◽

Processed Meat ◽

Pulsed Amperometric Detection ◽

Au Electrode ◽

Integrated Pulsed Amperometric Detection

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Determination of cyanide in bamboo shoots by microdiffusion combined with ion chromatography–pulsed amperometric detection

Royal Society Open Science ◽

10.1098/rsos.172128 ◽

2018 ◽

Vol 5 (4) ◽

pp. 172128 ◽

Cited By ~ 5

Author(s):

Ming Ding ◽

Kailiang Wang

Keyword(s):

Ion Chromatography ◽

Limit Of Detection ◽

Correlation Coefficients ◽

Amperometric Detection ◽

High Sensitivity ◽

Practical Method ◽

Pulsed Amperometric Detection ◽

Bamboo Shoots ◽

Relative Standard

A practical method for the determination of cyanide in bamboo shoots has been developed using microdiffusion preparation integrated with ion chromatography–pulsed amperometric detection (IC-PAD). Cyanide was released from bamboo shoots after Conway cell microdiffusion, and then analysed by IC-PAD. In comparison with the previously reported methods, derivatization and ion-pairing agent addition were not required in this proposed microdiffusion combined with IC-PAD method. The microdiffusion parameters were optimized including hydrolysis systems, temperature, time, and so on. Under the optimum conditions, the linear range of the calibration curve for cyanide was 0.2–200.0 µg kg −1 with satisfactory correlation coefficients of 0.9996 and the limit of detection was 0.2 µg kg −1 ( S/N = 3). The spiked recovery range was from 92.8 to 98.6%. The intra-day and inter-day relative standard deviations of cyanide were 2.7–14.9% and 3.0–18.3%, respectively. This method was proved to be convenient in operation with high sensitivity, precision and accuracy, and was successfully applied in the determination of cyanide in bamboo shoot samples.

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Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection

Journal of Analytical Methods in Chemistry ◽

10.1155/2017/7157953 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Ewa Jaszczak ◽

Marek Ruman ◽

Sylwia Narkowicz ◽

Jacek Namieśnik ◽

Żaneta Polkowska

Keyword(s):

Ion Chromatography ◽

Biological Samples ◽

Amperometric Detection ◽

Sample Pretreatment ◽

Cyanide Ion ◽

Pulsed Amperometric Detection ◽

Analytical Protocol ◽

Good Repeatability ◽

Optimized Conditions

A simple and accurate ion chromatography (IC) method with pulsed amperometric detection (PAD) was proposed for the determination of cyanide ion in urine, sweat, and saliva samples. The sample pretreatment relies on alkaline digestion and application of Dionex OnGuard II H cartridge. Under the optimized conditions, the method showed good linearity in the range of 1–100 μg/L for urine, 5–100 μg/L for saliva, and 3–100 μg/L for sweat samples with determination coefficients (R)>0.992. Low detection limits (LODs) in the range of 1.8 μg/L, 5.1 μg/L, and 5.8 μg/L for urine, saliva, and sweat samples, respectively, and good repeatability (CV < 3%, n=3) were obtained. The proposed method has been successfully applied to the analysis of human biological samples.

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