scholarly journals Different Fruit-Specific Promoters Drive AtMYB12 Expression to Improve Phenylpropanoid Accumulation in Tomato

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 317
Author(s):  
Xiangyu Ding ◽  
Ziyi Yin ◽  
Shaoli Wang ◽  
Haoqi Liu ◽  
Xiaomeng Chu ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Expression Vectors ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Myb Transcription Factor ◽  
Human Diet ◽  
Healthy Human ◽  
Total Antioxidant ◽  
Transgenic Lines ◽  
Trolox Equivalent ◽  
Transgenic Technologies

Tomato is an economically crucial vegetable/fruit crop globally. Tomato is rich in nutrition and plays an essential role in a healthy human diet. Phenylpropanoid, a critical compound in tomatoes, reduces common degenerative and chronic diseases risk caused by oxidative stress. As an MYB transcription factor, ATMYB12 can increase phenylpropanoid content by activating phenylpropanoid synthesis related genes, such as PAL, C4H, 4CL, CHS. However, the heterologous expression of AtMYB12 in tomatoes can be altered through transgenic technologies, such as unstable expression vectors and promoters with different efficiency. In the current study, the efficiency of other fruit-specific promoters, namely E8S, 2A12, E4, and PG, were compared and screened, and we determined that the expression efficiency of AtMYB12 was driven by the E8S promoter was the highest. As a result, the expression of phenylpropanoid synthesis related genes was regulated by AtMYB12, and the phenylpropanoid accumulation in transgenic tomato fruits increased 16 times. Additionally, the total antioxidant capacity of fruits was measured through Trolox equivalent antioxidant capacity (TEAC) assay, which was increased by 2.4 times in E8S transgenic lines. TEAC was positively correlated with phenylpropanoid content. Since phenylpropanoid plays a crucial role in the human diet, expressing AtMYB12 with stable and effective fruit-specific promoter E8S could improve tomato’s phenylpropanoid and nutrition content and quality. Our results can provide genetic resources for the subsequent improvement of tomato varieties and quality, which is significant for human health.

scholarly journals Effect of the French Oak Wood Extract Robuvit on Markers of Oxidative Stress and Activity of Antioxidant Enzymes in Healthy Volunteers: A Pilot Study

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Martina Horvathova ◽  
Zuzana Orszaghova ◽  
Lucia Laubertova ◽  
Magdalena Vavakova ◽  
Peter Sabaka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Healthy Volunteers ◽  
Total Antioxidant Capacity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Markers Of Oxidative Stress

We examinedin vitroantioxidant capacity of polyphenolic extract obtained from the wood of oakQuercus robur(QR), Robuvit, using TEAC (Trolox equivalent antioxidant capacity) method and the effect of its intake on markers of oxidative stress, activity of antioxidant enzymes, and total antioxidant capacity in plasma of 20 healthy volunteers. Markers of oxidative damage to proteins, DNA, and lipids and activities of Cu/Zn-superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were determined in the erythrocytes. We have found anin vitroantioxidant capacity of Robuvit of 6.37 micromole Trolox equivalent/mg of Robuvit. One month intake of Robuvit in daily dose of 300 mg has significantly decreased the serum level of advanced oxidation protein products (AOPP) and lipid peroxides (LP). Significantly increased activities of SOD and CAT as well as total antioxidant capacity of plasma after one month intake of Robuvit have been shown. In conclusion, we have demonstrated for the first time that the intake of Robuvit is associated with decrease of markers of oxidative stress and increase of activity of antioxidant enzymes and total antioxidant capacity of plasmain vivo.

Biochemical components of advanced selections and raspberry cultivars

2008 ◽  
Vol 88 (1) ◽  
pp. 175-178 ◽  
Author(s):  
B. Ehsani-Moghaddam ◽  
S. Khanizadeh ◽  
J. A. Sullivan
Keyword(s):  
Antioxidant Capacity ◽  
Rubus Idaeus ◽  
Soluble Solids ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Content ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Lipophilic Antioxidant ◽  
Solids Content ◽  
The University

Commercially grown raspberry cultivars (Rubus idaeus L.) (Festival, Boyne, Nova and Killarney) and advanced selections (88–18, 88–117, 88–134, SJR942-7) from the University of Guelph and Agriculture and Agri-Food Canada (AAFC) were evaluated for total antioxidant capacity, soluble solids content and acidity. The crude, hydrophilic and lipophilic antioxidant content of berries were measured using the Trolox equivalent antioxidant capacity (TEAC) method. Significant variations were observed among crude, hydrophilic and lipophilic antioxidant content of the different advanced selections. Selection 88–18, had the highest crude, hydrophilic and lipophilic antioxidant capacity, while SJR942-7 had the highest soluble solids. The study shows the importance of genetic background in determining the antioxidant potential in raspberry and the possibility of developing new lines rich in phytochemicals. Key words: Hydrophilic, lipophilic, total antioxidants, TEAC, Trolox, soluble solids

scholarly journals Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 992 ◽  
Author(s):  
Joanna Pokorska ◽  
Dominika Kułaj ◽  
Agata Piestrzyńska-Kajtoch ◽  
Anna Radko
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Lipocalin 2 ◽  
Holstein Friesian ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Daily Milk Yield ◽  
Friesian Cows

In the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.

scholarly journals Evaluation of the Bioaccessibility of Antioxidant Bioactive Compounds and Minerals of Four Genotypes of Brassicaceae Microgreens

Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 250 ◽  
Author(s):  
Beatriz de la Fuente ◽  
Gabriel López-García ◽  
Vicent Mañez ◽  
Amparo Alegría ◽  
Reyes Barberá ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Brassica Oleracea ◽  
Bioactive Compounds ◽  
Total Antioxidant Capacity ◽  
Dry Weight ◽  
Nutritional Composition ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Carotenoids ◽  
Total Antioxidant ◽  
Trolox Equivalent

Microgreens constitute an emerging class of fresh, healthy foods due to their nutritional composition. In this study the content of minerals and antioxidant bioactive compounds, and for the first time bioaccessibility, were evaluated in broccoli (Brassica oleracea L. var. italica Plenck), green curly kale (Brassica oleracea var. sabellica L.), red mustard (Brassica juncea (L.) Czern.) and radish (Raphanus sativus L.) hydroponic microgreens. Macro- (K, Ca, Mg) and oligo-elements (Fe, Zn), ascorbic acid, total soluble polyphenols, total carotenoids, total anthocyanins, total isothiocyanates and total antioxidant capacity (Trolox Equivalent Antioxidant Capacity and Oxygen Radical Absorbance Capacity) were determined before and after the standardized simulated gastrointestinal digestion process. All microgreens provided relevant amounts of vitamin C (31–56 mg/100 g fresh weight) and total carotenoids (162–224 mg β-carotene/100 g dry weight). Mineral content was comparable to that normally found in hydroponic microgreens and the low potassium levels observed would allow their dietetic recommendation for patients with impaired kidney function. Both total soluble polyphenols and total isothiocyanates were the greatest contributors to the total antioxidant capacity after digestion (43–70% and 31–63% bioaccessibility, respectively) while macroelements showed an important bioaccessibility (34–90%). In general, radish and mustard presented the highest bioaccessibility of bioactive compounds and minerals. Overall, the four hydroponic Brassicaceae microgreens present a wide array of antioxidant bioactive compounds.

A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates

1993 ◽  
Vol 84 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Nicholas J. Miller ◽  
Catherine Rice-Evans ◽  
Michael J. Davies ◽  
Vimala Gopinathan ◽  
Anthony Milner
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reference Population ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Compounds ◽  
Automated Method ◽  
Plasma Antioxidant Capacity ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Plasma Antioxidant

1. A new method has been developed for measuring the total antioxidant capacity of body fluids and drug solutions, based on the absorbance of the ABTS*+ radical cation. 2. An automated method for use on a centrifugal analyser, as well as a manual method, is described. 3. The procedure has been applied to physiological antioxidant compounds and radical-scavenging drugs, and an antioxidant ranking was established based on their reactivity relative to a 1.0 mmol/l Trolox standard. 4. The Trolox equivalent antioxidant capacity of plasma from an adult reference population has been measured, and the method optimized and validated. 5. The method has been applied to investigate the total plasma antioxidant capacity of neonates and how this may be compromised in prematurity.

scholarly journals Pomegranate Iron (III) Reducing Antioxidant Capacity (iRAC) Compared to ABTS Radical Quenching

2018 ◽  
Author(s):  
Hau Ching Wan ◽  
Richard Owusu-Apenten ◽  
Poonam Singh Nigam ◽  
Bushra Sultana
Keyword(s):  
Antioxidant Capacity ◽  
Geographic Origin ◽  
Food Composition ◽  
Pomegranate Juice ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Freeze Dried ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Food Composition Data ◽  
Pomegranate Fruit

Pomegranate juice (PJ) has total antioxidant capacity (TAC) which is reportedly higher compared to other common beverages. This short study aimed to evaluate the TAC of commercial PJ and pomegranate fruit in terms of a newly described iron (III) reducing antioxidant capacity (iRAC) and to compare with ABTS free radical quenching activity. Commercial PJ, freeze-dried pomegranate, and oven dried-pomegranate were analyzed. The total phenols content (TPC) was also assessed by the Folin-Ciocalteu method. The calibration results for iRAC were comparable to ABTS and Folin-Ciocalteu methods in terms of linearity (R2 > 0.99), sensitivity and precision. The TAC for PJ expressed as trolox equivalent antioxidant capacity (TEAC) was 33.4 ± 0.5 mM with the iRAC method and 36.3 ± 2.1 mM using the ABTS method. For dried pomegranates, TAC was 89–110 mmol/100 g or 76.0 ± 4.3 mmol/100 g using iRAC and ABTS methods, respectively. Freeze-dried pomegranate had 15% higher TAC compared with oven-dried pomegranate. In conclusion, pomegranate has high TAC as evaluated by the iRAC and ABTS methods, though variations occur due to the type of cultivar, geographic origin, processing and other factors. The study is relevant for attempts to refine food composition data for pomegranate and other functional foods.

scholarly journals Influence of Electronic Cigarettes on Antioxidant Capacity and Nucleotide Metabolites in Saliva

Toxics ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 263
Author(s):  
Dominika Cichońska ◽  
Oliwia Król ◽  
Ewa M. Słomińska ◽  
Barbara Kochańska ◽  
Dariusz Świetlik ◽  
...  
Keyword(s):  
Uric Acid ◽  
Antioxidant Capacity ◽  
Antioxidant Status ◽  
Electronic Cigarettes ◽  
Total Antioxidant Status ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Systems ◽  
Uric Acid Concentration ◽  
Total Antioxidant ◽  
Trolox Equivalent

The balance between reactive oxygen species production and the activity of antioxidant systems present in saliva is an important element in maintaining oral environment homeostasis. E-cigarettes adversely affect the oral cavity and their cytotoxic effect is related to oxidative stress. The aim of this study was to assess the influence of using electronic cigarettes on antioxidant capacity of saliva. The study involved 110 subjects (35 e-cigarettes users, 33 traditional cigarettes smokers and 42 non-smokers). Laboratory analysis involved quantitation of uric acid, hypoxanthine, xanthine, TAOS (total antioxidant status) and TEAC (Trolox equivalent antioxidant capacity) in saliva. Lower values for TAOS and TEAC were observed among e-cigarettes users and traditional cigarettes smokers in comparison to non-smokers. Uric acid concentration tended to be higher among e-cigarettes users while no differences in hypoxanthine and xanthine saliva concentrations were observed. Electronic cigarettes usage affects antioxidant capacity of saliva to the same extent as traditional cigarettes, when comparing smokers to non-smokers. Further longitudinal studies on a larger study group are needed to assess the effect of changes in antioxidant status on oral health.

scholarly journals ANTIOXIDANT CAPACITY AND TOTAL PHENOL CONTENT OF HYPTIS SPP., P. HEPTAPHYLLUM, T. PANAMENSIS, T. RHOIFOLIA, AND OCOTEA SP.

2016 ◽  
Vol 44 (2) ◽  
pp. 28-33 ◽  
Author(s):  
Geovanna Tafurt García ◽  
Luisa Jiménez Vidal ◽  
Ana Calvo Salamanca
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Colorimetric Assay ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Total Antioxidant ◽  
Trolox Equivalent ◽  
Highly Correlated

<p>In this work, the possible correlation between the antioxidant activities and the Total Phenolic Content (TPC) and chemical composition of Lamiaceae (<em>H. conferta, H. dilatata, H. mutabilis, H. suaveolens</em>), Burseraceae (<em>P. heptaphyllum, T. rhoifoila, T. panamensis</em>),<em> </em>and Lauraceae (<em>Ocotea </em>sp.) were evaluated. The Trolox Equivalent Antioxidant Capacity or the Total Antioxidant Activity (TAA) was determined by using a colorimetric assay with the ABTS radical cation, Effective Concentration (EC<sub>50</sub>) was evaluated with the DPPH radical, and the TPC was established by the Folin-Ciocalteu method, for ethanolic extracts obtained by cold maceration and evaporation to dryness. Both the TAA and the EC<sub>50</sub> were highly correlated with the TPC. The barks of <em>T. rhoifolia</em> and<em> T. panamensis</em> demonstrated the highest antioxidant capacities. The Burseraceae spp. exhibited the highest TPC, and the Lamiaceae (<em>Hyptis</em> spp.) demonstrated the lowest TPC.</p>

scholarly journals Comparison of Iron (III) Reducing Antioxidant Capacity (iRAC) and ABTS Radical Quenching Assays for Estimating Antioxidant Activity of Pomegranate

Beverages ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 58 ◽  
Author(s):  
Hau Wan ◽  
Bushra Sultana ◽  
Poonam Singh Nigam ◽  
Richard Owusu-Apenten
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Geographic Origin ◽  
Dry Weight ◽  
Food Composition ◽  
Pomegranate Juice ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Freeze Dried ◽  
Total Antioxidant ◽  
Trolox Equivalent

Pomegranate juice (PJ) has total antioxidant capacity which is reportedly higher compared to other common beverages. This short study aimed to assess the total antioxidant capacity of commercial PJ and pomegranate fruit using a newly described method for iron (III) reducing antioxidant capacity (iRAC) and to compare with the ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) and Folin–Ciocalteu assays. Commercial PJ, freeze-dried pomegranate, and oven-dried pomegranate were analyzed. The calibration results for iRAC were comparable to ABTS and Folin–Ciocalteu methods in terms of linearity (R2 > 0.99), sensitivity and precision. The total antioxidant capacity for PJ expressed as trolox equivalent antioxidant capacity (TEAC) was 33.4 ± 0.5 mM with the iRAC method and 36.3 ± 2.1 mM using the ABTS method. For dried pomegranates, total antioxidant capacity on a dry weight basis (DB) was 89–110 mmol/100 g DB or 76.0 ± 4.3 mmol/100 g DB using iRAC and ABTS methods, respectively. Freeze-dried pomegranate had 15% higher total antioxidant capacity compared with oven-dried pomegranate. In conclusion, pomegranate has high total antioxidant capacity as evaluated by the iRAC and ABTS methods, though variations occur due to the type of cultivar, geographic origin, processing and other factors. The study is relevant for attempts to refine food composition data for pomegranate and other functional foods.

Sign in / Sign up

Export Citation Format

Share Document