Lipid Profiles of the Heads of Four Shrimp Species by UPLC–Q–Exactive Orbitrap/MS and Their Cardiovascular Activities

Lipids are key factors in nutrition, structural function, metabolic features, and other biological functions. In this study, the lipids from the heads of four species of shrimp (Fenneropenaeus chinensis (FC), Penaeus japonicus (PJ), Penaeus vannamei (PV), and Procambarus clarkia (PCC)) were compared and characterized based on UPLC–Q–Exactive Orbitrap/MS. We compared the differences in lipid composition of four kinds of shrimp head using multivariate analysis. In addition, a zebrafish model was used to evaluate pro-angiogenic, anti-inflammatory, anti-thrombotic, and cardioprotective activities of the shrimp head lipids. The lipids from the four kinds of shrimp head had different degrees of pro-angiogenic activities, and the activities of PCC and PJ shrimp lipids were more significant than those of the other two species. Four lipid groups displayed strong anti-inflammatory activities. For antithrombotic activity, only PCC (25 μg/mL) and PV (100 μg/mL) groups showed obvious activity. In terms of cardioprotective activity, the four kinds of lipid groups significantly increased the zebrafish heart rhythms. The heart distances were shortened, except for those of the FC (100 μg/mL) and PJ (25 μg/mL) groups. Our comprehensive lipidomics analysis and bioactivity study of lipids from different sources could provide a basis for the better utilization of shrimp.

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UPLC-Q-Exactive Orbitrap/MS-Based Lipidomics Approach To Characterize Lipid Extracts from Bee Pollen and Their in Vitro Anti-Inflammatory Properties

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.7b02285 ◽

2017 ◽

Vol 65 (32) ◽

pp. 6848-6860 ◽

Cited By ~ 19

Author(s):

Qiangqiang Li ◽

Xinwen Liang ◽

Liang Zhao ◽

Zhongyin Zhang ◽

Xiaofeng Xue ◽

...

Keyword(s):

Bee Pollen ◽

Q Exactive ◽

Anti Inflammatory ◽

Orbitrap Ms

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Comparison of the Proximate Composition and Nutritional Profile of Byproducts and Edible Parts of Five Species of Shrimp

Foods ◽

10.3390/foods10112603 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2603

Author(s):

Zhenyang Liu ◽

Qiumei Liu ◽

Di Zhang ◽

Shuai Wei ◽

Qinxiu Sun ◽

...

Keyword(s):

Penaeus Monodon ◽

Macrobrachium Rosenbergii ◽

Fenneropenaeus Chinensis ◽

Meat Yield ◽

Penaeus Japonicus ◽

Chemical Score ◽

Shrimp Head ◽

Amino Acid Score ◽

Bioactive Ingredients ◽

Nutritional Benefits

The nutritional components of different parts (meat, head, shell and tail) of Litopenaeus vannamei (L.v), Macrobrachium rosenbergii (M.r), Penaeus monodon (P.m), Fenneropenaeus chinensis (F.c), and Penaeus japonicus (P.j) were analyzed and their nutritional values were evaluated. For the five species of shrimp, the meat yield was 37.47–55.94%, and the byproduct yield was 44.06–62.53%. The meat yields of L.v and F.c were the highest (55.94 and 55.92%, respectively), and the meat yield of M.r was the lowest (37.47%). The shrimp contain high amounts of crude protein, and the values of the amino acid score (AAS), chemical score (CS), and essential amino index (EAAI) were greater than or close to 1.00, indicating that shrimp protein had higher nutritional value. The shrimp head was rich in polyunsaturated fatty acids and the ratio of n-6 to n-3 PUFAs was from 0.37 to 1.68, indicating that the shrimp head is rich in n-3 PUFAs and is a good source of n-3 PUFAs. The five species of shrimp were rich in macro- and micro-minerals, especially in shrimp byproducts. The shrimp byproducts were also rich in other bioactive ingredients (astaxanthin), which are also very valuable for developing biological resources. Therefore, shrimp have many nutritional benefits, and their byproducts can also be used to develop natural nutraceuticals, which are considered to be one of the healthiest foods.

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The analysis of active compounds in Flos Chrysanthemi Indici by UHPLC Q exactive HF hybrid Quadrupole-Orbitrap MS and comprehensive quality assessment of its preparation

Food & Function ◽

10.1039/d0fo03053h ◽

2021 ◽

Author(s):

Tingting Dai ◽

Guoxiang Sun

Keyword(s):

Antioxidant Activity ◽

Correlation Analysis ◽

Quality Assessment ◽

Active Compounds ◽

Q Exactive ◽

Orbitrap Ms

Active compounds’ analysis between Flos Chrysanthemi Indici (FCI) and its preparation (FCIP), and the comprehensive quality assessment of its preparation as well as the correlation analysis between the antioxidant activity and fingerprint.

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Research on saponin active compounds of Tuchao Baibiandouren for the treatment of type-2 diabetes based on UHPLC-Q-Exactive Orbitrap MS and network pharmacology

Digital Chinese Medicine ◽

10.1016/j.dcmed.2021.03.003 ◽

2021 ◽

Vol 4 (1) ◽

pp. 19-31

Author(s):

Fang Liangzi ◽

Zheng Qinfang ◽

Han Jun

Keyword(s):

Type 2 Diabetes ◽

Network Pharmacology ◽

Active Compounds ◽

Q Exactive ◽

Orbitrap Ms

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High-Throughput Identification of Organic Compounds from Polygoni Multiflori Radix Praeparata (Zhiheshouwu) by UHPLC-Q-Exactive Orbitrap-MS

Molecules ◽

10.3390/molecules26133977 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3977

Author(s):

Shaoyun Wang ◽

Xiaozhu Sun ◽

Shuo An ◽

Fang Sang ◽

Yunli Zhao ◽

...

Keyword(s):

High Performance ◽

Chemical Constituents ◽

Water Extract ◽

Ethanol Extract ◽

In Vivo Studies ◽

Polygonum Multiflorum ◽

Q Exactive ◽

Orbitrap Ms

Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.

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Identification of Components in Citri Sarcodactylis Fructus from Different Origins via UPLC-Q-Exactive Orbitrap/MS

ACS Omega ◽

10.1021/acsomega.1c02124 ◽

2021 ◽

Author(s):

Kanghui Wang ◽

Jingyuan Tian ◽

Yueshan Li ◽

Mengshi Liu ◽

Yingxin Chao ◽

...

Keyword(s):

Q Exactive ◽

Different Origins ◽

Orbitrap Ms

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Protective Effect of Phillyrin on Lethal LPS-Induced Neutrophil Inflammation in Zebrafish

Cellular Physiology and Biochemistry ◽

10.1159/000484192 ◽

2017 ◽

Vol 43 (5) ◽

pp. 2074-2087 ◽

Cited By ~ 19

Author(s):

Liling Yang ◽

Xiangjun Zhou ◽

Weijuan Huang ◽

Qin Fang ◽

Jianlan Hu ◽

...

Keyword(s):

Signalling Pathway ◽

Dependent Manner ◽

Zebrafish Model ◽

Lps Challenge ◽

Cell Counts ◽

Tnf Α ◽

Forsythia Suspensa ◽

Anti Inflammatory

Background/Aims: Forsythia suspensa Vahl. (Oleaceae) fruits are widely used in traditional Chinese medicine to treat pneumonia, typhoid, dysentery, ulcers and oedema. Antibacterial and anti-inflammatory activities have been reported for phillyrin (PHN), the main ingredient in Forsythia suspensa Vahl fruits, in vitro. However, the underlying mechanisms in vivo remain poorly defined. In this study, we discovered that PHN exerted potent anti-inflammatory effects in lethal LPS-induced neutrophil inflammation by suppressing the MyD88-dependent signalling pathway in zebrafish. Methods: LPS-yolk microinjection was used to induce a lethal LPS-infected zebrafish model. The effect of PHN on the survival of zebrafish challenged with lethal LPS was evaluated using survival analysis. The effect of PHN on neutrophil inflammation grading in vivo was assessed by tracking neutrophils with a transgenic line. The effects of PHN on neutrophil production and migration were analysed by SB+ cell counts during consecutive hours after modelling. Additionally, key cytokines and members of the MyD88 signalling pathway that are involved in inflammatory response were detected using quantitative RT-PCR. To assess gene expression changes during consecutive hours after modelling, the IL-1β, IL-6, TNF-α, MyD88, TRIF, ERK1/2, JNK, IκBa and NF-κB expression levels were measured. Results: PHN could protect zebrafish against a lethal LPS challenge in a dose-dependent manner, as indicated by decreased neutrophil infltration, reduced tissue necrosis and increased survival rates. Up-regulated IL-1β, IL-6 and TNF-α expression also showed the same tendencies of depression by PHN. Critically, PHN significantly inhibited the LPS-induced activation of MyD88, IκBa, and NF-κB but did not affect the expression of ERK1/2 MAPKs or JNK MAPKs in LPS-stimulated zebrafish. Additionally, PHN regulated the MyD88/IκBα/NF-κB signalling pathway by controlling IκBα, IL-1β, IL-6, and TNF-α expression. Conclusion: This study provides a rationale for the clinical application of PHN as an anti-inflammatory agent.

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Characterization of Metabolites of α-mangostin in Bio-samples from SD Rats by UHPLC-Q-Exactive Orbitrap MS

Current Drug Metabolism ◽

10.2174/1389200222666211126093124 ◽

2021 ◽

Vol 22 ◽

Author(s):

Fan Dong ◽

Shaoping Wang ◽

Ailin Yang ◽

Haoran Li ◽

Pingping Dong ◽

...

Keyword(s):

Metabolic Pathways ◽

Garcinia Mangostana ◽

Sd Rats ◽

Metabolic Route ◽

Wide Range ◽

New Strategy ◽

Q Exactive ◽

Orbitrap Ms

Background: α-mangostin, a typical xanthone, often exists in Garcinia mangostana L. (Clusiaceae). α-mangostin was found to have a wide range of pharmacological properties. However, its specific metabolic route in vivo remains unclear, while these metabolites may accumulate to exert pharmacological effects, too. Objective: This study aimed to clarify the metabolic pathways of α-mangostin after oral administration to the rats. Methods: Here, an UHPLC-Q-Exactive Orbitrap MS was used for the detection of potential metabolites formed in vivo. A new strategy for the identification of unknown metabolites based on typical fragmentation routes was implemented. Results: A total of 42 metabolites were detected, and their structures were tentatively identified in this study. The results showed that major in vivo metabolic pathways of α-mangostin in rats included methylation, demethylation, methoxylation, hydrogenation, dehydrogenation, hydroxylation, dehydroxylation, glucuronidation, and sulfation. Conclusions: This study is significant to expand our knowledge of the in vivo metabolism of α-mangostin and to understand the mechanism of action of α-mangostin in rats in vivo.

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Geographic Marketing in Support of Decision-Making Processes

Advances in Business Strategy and Competitive Advantage - Digital Marketing Strategies and Models for Competitive Business ◽

10.4018/978-1-7998-2963-8.ch005 ◽

2020 ◽

pp. 103-115

Author(s):

Maria Fernanda Augusto

Keyword(s):

Decision Making ◽

Geographic Information ◽

Essential Elements ◽

Added Value ◽

Key Factors ◽

Digital Maps ◽

Decision Making Processes ◽

Growth Prospects ◽

Definition Of ◽

Different Sources

Nowadays, geographic information and spatial aspects are essential elements for the definition of companies' strategies. With the use of different sources data, companies were able to obtain insights that they could not obtain without the spatial component and were able to use them to optimize their business. Then, geographic marketing presents itself as an added value for companies, one of the key factors being its role in supporting decision making. The main attributes of geographic marketing or GeoMarketing allow us to identify and present through digital maps the behavior and trends of certain variables based on characteristics of a market. The meticulous study of spatial and demographic information generated by GeoMarketing are crucial for important strategic adjustments in the business plan, such as definitions related to the location considered ideal for the business, target audience, price and growth prospects, among other factors. In this context, GeoMarketing will be introduced, exploring its scope, applicability, and relevance of its use in support of the decision-making process.

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