scholarly journals Application of Selected Biomaterials and Stem Cells in the Regeneration of Hard Dental Tissue in Paediatric Dentistry—Based on the Current Literature

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3374
Author(s):  
Alina Wrzyszcz-Kowalczyk ◽  
Maciej Dobrzynski ◽  
Iwona Grzesiak-Gasek ◽  
Wojciech Zakrzewski ◽  
Monika Mysiak-Debska ◽  
...  
Keyword(s):  
Stem Cell ◽  
Tissue Regeneration ◽  
Paediatric Dentistry ◽  
Pulp Tissue ◽  
Dental Tissue ◽  
Ongoing Research ◽  
Dental Tissues ◽  
Regenerative Dentistry ◽  
Hard Dental Tissues ◽  
New Research

Currently, the development of the use of biomaterials and their application in medicine is causing rapid changes in the fields of regenerative dentistry. Each year, new research studies allow for the discovery of additional possibilities of dental tissue restoration. The structure and functions of teeth are complex. They consist of several diverse tissues that need to act together to ensure the tooth’s function and durability. The integrity of a tooth’s enamel, dentin, cementum, and pulp tissue allows for successful mastication. Biomaterials that are needed in dentistry must withstand excessive loading forces, be biocompatible with the hosts’ tissues, and stable in the oral cavity environment. Moreover, each tooth’s tissue, as well as aesthetic qualities in most cases, should closely resemble the natural dental tissues. This is why tissue regeneration in dentistry is such a challenge. This scientific research focuses on paediatric dentistry, its classification of caries, and the use of biomaterials in rebuilding hard dental tissues. There are several methods described in the study, including classical conservative methods such as caries infiltration or stainless-steel crowns. Several clinical cases are present, allowing a reader to better understand the described methods. Although the biomaterials mentioned in this work are artificial, there is currently ongoing research regarding clinical stem cell applications, which have a high potential for becoming one of the most common techniques of lost dental-tissue regeneration in the near future. The current state of stem cell development is mentioned, as well as the various methods of its possible application in dentistry.

scholarly journals Hard Dental Tissues Regeneration—Approaches and Challenges

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2558
Author(s):  
Mihaela Olaru ◽  
Liliana Sachelarie ◽  
Gabriela Calin
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Present Review ◽  
Modern Concept ◽  
Dental Tissues ◽  
Tissue Engineering Approach ◽  
Hard Dental Tissues ◽  
Review Reports

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

scholarly journals Er:YAG Laser Dental Treatment of Patients Affected by Epidermolysis Bullosa

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Angela Galeotti ◽  
Vincenzo D’Antò ◽  
Tina Gentile ◽  
Alexandros Galanakis ◽  
Simona Giancristoforo ◽  
...  
Keyword(s):  
Epidermolysis Bullosa ◽  
Dental Treatment ◽  
Er:Yag Laser ◽  
Enamel Hypoplasia ◽  
Dental Tissue ◽  
Maxillary Canine ◽  
Dental Tissues ◽  
First Case ◽  
Hard Dental Tissue ◽  
Hard Dental Tissues

Aim. The purpose of this study was to evaluate the efficacy of Er:YAG laser used for treating hard dental tissue in patients with epidermolysis bullosa (EB).Methods. We report two cases of EB in which an Er:YAG laser was used for conservative treatments. In the first case, the Er:YAG laser (2,940 μm, 265 mJ, 25 Hz) was used to treat caries on a deciduous maxillary canine in an 8-year-old male patient affected by dystrophic EB. In the second case, we treated a 26-year-old female patient, affected by junctional EB, with generalized enamel hypoplasia, and an Er:YAG laser (2,940 μm, 265 mJ, 25 Hz) was used to remove the damaged enamel on maxillary incisors.Results. The use of the Er:YAG laser, with the appropriate energy, was effective in the selective removal of carious tissue and enamel hypoplasia. During dental treatment with the Er:YAG laser, patients required only a few interruptions due to the absence of pain, vibration, and noise.Conclusions. Laser treatment of hard dental tissues is a valuable choice for patients affected by EB since it is less invasive compared to conventional treatment, resulting in improved patient compliance.

scholarly journals Tooth Formation: Are the Hardest Tissues of Human Body Hard to Regenerate?

2020 ◽  
Vol 21 (11) ◽  
pp. 4031 ◽  
Author(s):  
Juliana Baranova ◽  
Dominik Büchner ◽  
Werner Götz ◽  
Margit Schulze ◽  
Edda Tobiasch
Keyword(s):  
Stem Cells ◽  
Drug Release ◽  
Adult Stem Cells ◽  
Complex Structure ◽  
Tooth Germ ◽  
Tooth Regeneration ◽  
Dental Tissue ◽  
Dental Tissues ◽  
Regenerative Dentistry

With increasing life expectancy, demands for dental tissue and whole-tooth regeneration are becoming more significant. Despite great progress in medicine, including regenerative therapies, the complex structure of dental tissues introduces several challenges to the field of regenerative dentistry. Interdisciplinary efforts from cellular biologists, material scientists, and clinical odontologists are being made to establish strategies and find the solutions for dental tissue regeneration and/or whole-tooth regeneration. In recent years, many significant discoveries were done regarding signaling pathways and factors shaping calcified tissue genesis, including those of tooth. Novel biocompatible scaffolds and polymer-based drug release systems are under development and may soon result in clinically applicable biomaterials with the potential to modulate signaling cascades involved in dental tissue genesis and regeneration. Approaches for whole-tooth regeneration utilizing adult stem cells, induced pluripotent stem cells, or tooth germ cells transplantation are emerging as promising alternatives to overcome existing in vitro tissue generation hurdles. In this interdisciplinary review, most recent advances in cellular signaling guiding dental tissue genesis, novel functionalized scaffolds and drug release material, various odontogenic cell sources, and methods for tooth regeneration are discussed thus providing a multi-faceted, up-to-date, and illustrative overview on the tooth regeneration matter, alongside hints for future directions in the challenging field of regenerative dentistry.

scholarly journals Thermal Changes in the Hard Dental Tissue at Diode Laser Root Canal Treatment

2014 ◽  
Vol 41 (2) ◽  
pp. 31-35
Author(s):  
Ts. Uzunov ◽  
R. Grozdanova ◽  
E. Popova ◽  
T. Uzunov
Keyword(s):  
Laser Treatment ◽  
Diode Laser ◽  
Root Canal ◽  
In Vitro Study ◽  
Laser Coagulation ◽  
Dental Tissue ◽  
Dental Tissues ◽  
Thermal Changes ◽  
Hard Dental Tissues

Summary The laser coagulation at the apical part of the root canal after vital extirpation is a proper method of preventing complications such as pain, bleeding, remaining vital pulp. The aim of the present survey is to register the thermal changes that occur on the tooth surfaces during laser treatment of the root canal after vital extirpation. An in vitro study of 30 extracted teeth has been conducted. The teeth have been prepared with ProTaper nickel-titanium machine tools and wiped dry. During the course of laser treatment of root canals with a diode laser DenLase temperatures, changes of the surface of the hard dental tissues have been recorded with infrared camera FLIR T330. The captured thermal images have been processed with software product Flir Reporter Pro 9. In conclusion, temperature changes in hard dental tissues at diode laser treatment of the root canal are biocompatible.

scholarly journals In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration

2021 ◽  
Vol 11 (18) ◽  
pp. 8472
Author(s):  
Ik-Hwan Kim ◽  
Mijeong Jeon ◽  
Kyounga Cheon ◽  
Sun Ha Kim ◽  
Han-Sung Jung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Tooth Development ◽  
Healing Process ◽  
Permanent Teeth ◽  
Human Tooth ◽  
Pulp Tissue ◽  
Dental Tissue ◽  
In Vivo Evaluation

Conventional root canal treatment may result in loss of tooth vitality, which can lead to unfavorable treatment outcomes. Notably, a ceased tooth development of immature permanent teeth with open apices, regeneration of periodontal ligaments (PDL), and pulp is highly expected healing process. For regeneration, the scaffold is one of the critical components that carry biological benefits. Therefore, this study evaluated a decellularized human tooth as a scaffold for the PDL and pulp tissue regeneration. A tooth scaffold was fabricated using an effective decellularization method as reported in previous studies. PDL stem cells (PDLSCs) and dental pulp stem cells (DPSCs) obtained from human permanent teeth were inoculated onto decellularized scaffolds, then cultured to transplant into immunosuppressed mouse. After 9 weeks, PDLSCs and DPSCs that were inoculated onto decellularized tooth scaffolds and cultured in an in vivo demonstrated successful differentiation. In PDLSCs, a regeneration of the cementum/PDL complex could be expected. In DPSCs, the expression of genes related to revascularization and the hard tissue regeneration showed the possibility of pulp regeneration. This study suggested that the potential possible application of decellularized human tooth could be a scaffold in regeneration PDL and pulp tissue along with PDLSCs and DPSCs, respectively, as a novel treatment method.

scholarly journals Tooth Bioengineering and Regenerative Dentistry

2019 ◽  
Vol 98 (11) ◽  
pp. 1173-1182 ◽  
Author(s):  
P.C. Yelick ◽  
P.T. Sharpe
Keyword(s):  
Tissue Engineering ◽  
Current Knowledge ◽  
Basic Research ◽  
Dental Tissue ◽  
Dental Tissues ◽  
The Past ◽  
Tremendous Progress ◽  
Regenerative Dentistry ◽  
Dental Tissue Engineering ◽  
Made In

Over the past 100 y, tremendous progress has been made in the fields of dental tissue engineering and regenerative dental medicine, collectively known as translational dentistry. Translational dentistry has benefited from the more mature field of tissue engineering and regenerative medicine (TERM), established on the belief that biocompatible scaffolds, cells, and growth factors could be used to create functional, living replacement tissues and organs. TERM, created and pioneered by an interdisciplinary group of clinicians, biomedical engineers, and basic research scientists, works to create bioengineered replacement tissues that provide at least enough function for patients to survive until donor organs are available and, at best, fully functional replacement organs. Ultimately, the goal of both TERM and regenerative dentistry is to bring new and more effective therapies to the clinic to treat those in need. Very recently, the National Institutes of Health/National Institute of Dental and Craniofacial Research invested $24 million over a 3-y period to create dental oral and craniofacial translational resource centers to facilitate the development of more effective therapies to treat edentulism and other dental-related diseases over the next decade. This exciting era in regenerative dentistry, particularly for whole-tooth tissue engineering, builds on many key successes over the past 100 y that have contributed toward our current knowledge and understanding of signaling pathways directing natural tooth and dental tissue development—the foundation for current strategies to engineer functional, living replacement dental tissues and whole teeth. Here we use a historical perspective to present key findings and pivotal advances made in the field of translational dentistry over the past 100 y. We will first describe how this process has evolved over the past 100 y and then hypothesize on what to expect over the next century.

Odontoblast Cell Death Drives Stem Cell-Independent Cell-Rich Zone-Derived Dental Tissue Regeneration

2020 ◽  
Author(s):  
Lijuan Zhao ◽  
Atsushi Arai ◽  
Nobuyuki Udagawa ◽  
Kanji Horibe ◽  
Miroku Hara ◽  
...  
Keyword(s):  
Cell Death ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Dental Tissue

scholarly journals Neural Crest Stem Cells from Dental Tissues: A New Hope for Dental and Neural Regeneration

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Gaskon Ibarretxe ◽  
Olatz Crende ◽  
Maitane Aurrekoetxea ◽  
Victoria García-Murga ◽  
Javier Etxaniz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Therapy ◽  
Neural Crest ◽  
Neural Regeneration ◽  
Dental Tissues ◽  
Adult Human ◽  
Regenerative Dentistry ◽  
Cord Injury

Several stem cell sources persist in the adult human body, which opens the doors to both allogeneic and autologous cell therapies. Tooth tissues have proven to be a surprisingly rich and accessible source of neural crest-derived ectomesenchymal stem cells (EMSCs), which may be employed to repair disease-affected oral tissues in advanced regenerative dentistry. Additionally, one area of medicine that demands intensive research on new sources of stem cells is nervous system regeneration, since this constitutes a therapeutic hope for patients affected by highly invalidating conditions such as spinal cord injury, stroke, or neurodegenerative diseases. However, endogenous adult sources of neural stem cells present major drawbacks, such as their scarcity and complicated obtention. In this context, EMSCs from dental tissues emerge as good alternative candidates, since they are preserved in adult human individuals, and retain both high proliferation ability and a neural-like phenotypein vitro. In this paper, we discuss some important aspects of tissue regeneration by cell therapy and point out some advantages that EMSCs provide for dental and neural regeneration. We will finally review some of the latest research featuring experimental approaches and benefits of dental stem cell therapy.

scholarly journals Modern View on the Problem of Treating Minor Dentition Defects with the Use of Zirconium Oxide Ceramic Resin-Bonded Prostheses

2019 ◽  
Vol 25 (1) ◽  
Author(s):  
Iryna Kumgyr ◽  
Zinoviy Ozhogan
Keyword(s):  
Zirconium Oxide ◽  
Oxide Ceramic ◽  
Tissue Preparation ◽  
Dental Tissue ◽  
Lateral Region ◽  
Dental Tissues ◽  
Results Of Treatment ◽  
Aesthetic Appearance ◽  
Hard Dental Tissues ◽  
The Aesthetic

The objective of the research was to substantiate the effective method of treating minor dentition defects in the lateral and frontal areas considering anatomic features, functionality and aesthetic appearance of the prosthesis.          Materials and methods. A new approach to the problem of treating minor dentition defects with the use of zirconium oxide ceramic resin-bonded prostheses was proposed. The method of its manufacturing was described and the indications to the application were clearly explained. The comparative analysis with similar prostheses was conducted and the advantages of the proposed prosthesis were proved. Ceramic resin-bonded prosthesis allowed us to restore the dentition integrity in the presence of small bounded edentulous spaces in the lateral region and to provide high aesthetic qualities and bio-inertness of the prosthesis with maximum approximation to the physiological characteristics of natural teeth.     Results. Thirty-eight patients with small bounded edentulous space in the lateral region were treated with the use of zirconium oxide resin-bonded prosthesis for 18 months. The obtained results of prosthetic treatment indicated that the proposed prosthesis provided an opportunity to restore the aesthetic appearance and integrity of the dentition, to prevent the occurrence of the deformations and to ensure the functionality of the dento-facial system.          Conclusions. The framework made of zirconium oxide allowed us to significantly reduce the depth of hard dental tissue preparation resulting in a great aesthetic appearance thereby providing an opportunity to significantly expand the indications for use. The remote results of treatment indicated the absence of marginal coloration at the margin of indirect restoration and hard dental tissues, namely the marginal ridge, ensuring good aesthetics and functional integrity of the restorative construction.

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