scholarly journals Molecular Characterization of German Acinetobacter baumannii Isolates and Multilocus Sequence Typing (MLST) Analysis Based on WGS Reveals Novel STs

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 690
Author(s):  
Gamal Wareth ◽  
Jörg Linde ◽  
Philipp Hammer ◽  
Wolf D. Splettstoesser ◽  
Mathias W. Pletz ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multilocus Sequence Typing ◽  
Human Origin ◽  
Mlst Analysis ◽  
Maldi Tof ◽  
Carbapenemase Gene ◽  
Strain Type ◽  
Significant Health ◽  
New Alleles

Acinetobacter baumannii (A. baumannii) is a major cause of severe nosocomial infections worldwide. The emergence of infections associated with A. baumannii poses a significant health risk in Germany. A. baumannii is part of the ACB complex and is difficult to distinguish from other species phenotypically, necessitating its reliable identification. The current study analyzed 89 A. baumannii strains from human and non-human origins by matrix-assisted laser desorption/ionization (MALDI–TOF) and PCR detection of intrinsic blaOXA-51-like carbapenemase, blaOXA-23-like, blaOXA-24-like, blaOXA-58-like, and ISAba 1 genes. Whole-genome sequencing (WGS) was applied for species confirmation and strain type determination. Combining the molecular detection of the intrinsic blaOXA-51-like carbapenemase gene together with MALDI–TOF with a score value of >2.300 proved to be a suitable tool for A. baumannii identification. WGS data for all of the sequenced strains confirmed the identity of all A. baumannii strains. The Pasteur scheme successfully assigned 79.7% of the strains into distinct STs, while the Oxford scheme succeeded in allocating only 42.7% of isolates. Multilocus sequence typing (MLST) analysis based on the Pasteur scheme identified 16 STs. ST/241 was the most prevalent in samples from non-human origin, whereas ST/2 was predominant in human samples. Furthermore, eight isolates of non-human origin were allocated to seven new STs (ST/1410, ST/1414, ST/1416, ST/1417, ST/1418, ST/1419, and ST/1421). Ten isolates from non-human origin could not be typed since new alleles were observed in the loci Pas_cpn60, Pas_rpoB, and Pas_gltA. MLST analysis based on the Pasteur scheme was more appropriate than the Oxford scheme for the current group of A. baumannii.

scholarly journals Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii

2005 ◽  
Vol 43 (9) ◽  
pp. 4382-4390 ◽  
Author(s):  
S. G. Bartual ◽  
H. Seifert ◽  
C. Hippler ◽  
M. A. D. Luzon ◽  
H. Wisplinghoff ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multilocus Sequence Typing ◽  
Clinical Isolates ◽  
Typing Scheme

Characterization of Neisseria meningitidis isolates collected from 1974 to 2003 in Japan by multilocus sequence typing

2004 ◽  
Vol 53 (7) ◽  
pp. 657-662 ◽  
Author(s):  
Hideyuki Takahashi ◽  
Toshiro Kuroki ◽  
Yuko Watanabe ◽  
Hiroshi Tanaka ◽  
Hiroo Inouye ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Neisseria Meningitidis ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
The Other ◽  
Mlst Database ◽  
Mlst Analysis ◽  
The Past ◽  
Sequence Types

Analysis of 182 Neisseria meningitidis strains isolated over the past 30 years in Japan by serogroup typing and multilocus sequence typing (MLST) was performed. The serogroups of the 182 Japanese isolates were B (103 isolates), Y (39), W135 (1) and non-groupable (39). By MLST analysis, 65 different sequence types (ST) were identified, 42 of which were not found in the MLST database as of January 2004 and seemed to be unique to Japan. Statistical analysis of the MLST results revealed that, although the Japanese isolates seemed to be genetically divergent, they were classified into six major clonal complexes and other minor complexes. Among these isolates, well-documented ST complexes found worldwide were present, such as ST-23 complex (49 isolates), ST-44 complex (41 isolates) and ST-32 complex (8 isolates). On the other hand, a new clonal complex designated ST-2046 complex (28 isolates), which has not been identified in other countries, was also found, suggesting that this clone was indigenous to Japan. Taken together, it was speculated that meningococcal isolates in Japan comprised heterogeneous clones, which were derived both from clones identified in other countries and clones unique to Japan.

scholarly journals Molecular Characterization and Antibiotic Resistance of Acinetobacter baumannii in Cerebrospinal Fluid and Blood

2020 ◽  
Author(s):  
Xiaohong Shi ◽  
Hong Wang ◽  
Xin Wang ◽  
Huaiqi Jing ◽  
Ran Duan ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Infection Control ◽  
Acinetobacter Baumannii ◽  
Multilocus Sequence Typing ◽  
Detection Rate ◽  
Genetic Relatedness ◽  
Carbapenem Resistance ◽  
Clinical Treatment ◽  
Control Measures ◽  
Carbapenemase Gene

Abstract BackgroundThe increasing rates of carbapenem-resistant Acinetobacter baumannii (CRAB) caused nosocomial infections generate significant comorbidity and sometime cause death among patients. Current treatment options are limited. These infections pose great difficulties for infection control and clinical treatment. This study identifies the antimicrobial resistance, carbapenemases and genetic relatedness of A. baumannii isolates from cerebrospinal fluid (CSF) and blood in a hospital in Shandong, China. Methods A total of 50 nonrepetitive CSF A. baumannii isolates and 44 blood isolates were collected. The resistance phenotypes were determined according to the Clinical and Laboratory Standards Institute guidelines. We performed Polymerase Chain Reaction (PCR) experiments to detect the carbapenem resistance mechanism. Finally, we conducted Multilocus Sequence Typing (MLST) to depict the genetic relatedness of these isolates. Results We observed that eighty-eight of the 94 isolates collected were resistant to imipenem or meropenem. Among them, the bla OXA-23 gene was the most prevalent carbapenemase gene with a 91.5% (86/94) detection rate, followed by the bla OXA-24 gene that showed a 2.1% (2/94) detection rate isolates. Among all CRAB observations in this study, isolates with the bla OXA-23 gene were resistant to both imipenem and meropenem. However, isolates positive for the bla OXA-24 gene but negative for the bla OXA-23 gene showed an imipenem-sensitive but meropenem-resistant phenotype. The outcome of multilocus sequence typing analysis showed 21 different STs were distinguished, of which ST195 (25.5%), ST540 (12.8%) and ST208 (11.7%) were most frequently observed. Eighty of the 94 isolates (85.1%) were clustered into CC92, and all CC92 isolates showed a carbapenem resistance phenotype (except AB13). Five novel STs were detected, and most of them were CRAB, some of which belonged to CC92. Conclusion A high level of carbapenem resistance was detected in this study. The CC92 and bla OXA-23 gene were predominant. Five novel STs were detected, and these new STs require further investigation to understand the nature of and to prevent outbreaks caused by A. baumannii . Our study provides additional observations and epidemiological data of CSF and blood A. baumannii strains, which may improve future infection control measures and aid in potential clinical treatment in hospitals and other clinical settings.

scholarly journals Identification of AbaR4 Acinetobacter baumannii resistance island in clinical isolates of blaOXA-23-positive Proteus mirabilis

2019 ◽  
Vol 75 (3) ◽  
pp. 521-525 ◽  
Author(s):  
Sophie Octavia ◽  
Weizhen Xu ◽  
Oon Tek Ng ◽  
Kalisvar Marimuthu ◽  
Indumathi Venkatachalam ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Proteus Mirabilis ◽  
National Surveillance ◽  
Class D ◽  
Oxford Nanopore ◽  
Carbapenemase Gene ◽  
Long Read ◽  
Encoding Gene ◽  
Resistance Island

Abstract Objectives bla OXA-23 is a class D carbapenemase-encoding gene typical of the Acinetobacter genus. However, its occurrence in the Enterobacteriaceae is uncommon. Here we provide the genome characterization of blaOXA-23-positive Proteus mirabilis. Methods In Singapore, a national surveillance of carbapenem non-susceptible clinical Enterobacteriaceae has enabled the collection of OXA-23 bearing isolates. Three clinical P. mirabilis were whole-genome sequenced using Oxford Nanopore MinION and Illumina platforms. The sequence accuracy of MinION long-read contigs was enhanced by polishing with Illumina-derived short-read data. Results In two P. mirabilis genomes, blaOXA-23 was detected as two copies, present on the chromosome and on a 60018 bp plasmid. blaOXA-23 was associated with the classic Acinetobacter composite transposon Tn2006, bounded by two copies of ISAba1 bracketing the carbapenemase gene. The Tn2006 itself was embedded within an Acinetobacter baumannii AbaR4 resistance island. In the chromosome, the AbaR4 was found integrated into the comM gene, which is also the preferred ‘hotspot’ in A. baumannii. In the plasmid, AbaR4 integrated into a putative colicin gene. Conclusions Our description of an A. baumannii AbaR4 encoding blaOXA-23 in P. mirabilis is to our knowledge the first description of an Acinetobacter resistance island in Proteus and suggests that P. mirabilis may be a reservoir for this class D carbapenemase gene.

Multilocus sequence typing and molecular characterization of β-lactamase genes among Acinetobacter baumannii isolates in a burn center

Burns ◽  
2017 ◽  
Vol 43 (7) ◽  
pp. 1473-1478 ◽  
Author(s):  
Guangtao Huang ◽  
Yuan Peng ◽  
Yong Yang ◽  
Chengyong Tang ◽  
Yuexian Fu
Keyword(s):  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Multilocus Sequence Typing ◽  
Burn Center

scholarly journals First Human Isolate of Salmonella enterica Serotype Enteritidis HarboringblaCTX-M-14in South America

2012 ◽  
Vol 56 (4) ◽  
pp. 2132-2134 ◽  
Author(s):  
Inés Bado ◽  
Virginia García-Fulgueiras ◽  
Nicolás F. Cordeiro ◽  
Laura Betancor ◽  
Leticia Caiata ◽  
...  
Keyword(s):  
South America ◽  
Clinical Isolate ◽  
Multilocus Sequence Typing ◽  
Salmonella Enterica ◽  
Conjugative Plasmid ◽  
Pcr Analysis ◽  
Human Origin ◽  
Content Type ◽  
Mlst Analysis ◽  
M 14

ABSTRACTWe studied a clinical isolate ofSalmonella entericaserotype Enteritidis showing resistance to oxyiminocephalosporins. PCR analysis confirmed the presence ofblaCTX-M-14linked to IS903in a 95-kb IncI1 conjugative plasmid. Such a plasmid is maintained on account of the presence of apndACaddiction system. Multilocus sequence typing (MLST) analysis indicated that the strain belongs to ST11. This is the first report ofblaCTX-M-14inSalmonellaEnteritidis of human origin in South America.

scholarly journals Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii

2007 ◽  
Vol 45 (6) ◽  
pp. 2101-2101 ◽  
Author(s):  
S. G. Bartual ◽  
H. Seifert ◽  
C. Hippler ◽  
M. A. D. Luzon ◽  
H. Wisplinghoff ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multilocus Sequence Typing ◽  
Clinical Isolates ◽  
Typing Scheme

Characterization of Healthy and Tumor Oral Cell Lines of Human Origin. The preliminary stage in the assessment of relevant chemical compounds with impact on dentistry

2018 ◽  
Vol 69 (10) ◽  
pp. 2889-2894
Author(s):  
Ion Virgil Corlan ◽  
Adelina Cheveresan ◽  
Delia Berceanu Vaduva ◽  
Cristian Nica ◽  
Alin Faur ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Chemical Compounds ◽  
Tongue Squamous Cell Carcinoma ◽  
Gingival Fibroblasts ◽  
Human Origin ◽  
Preliminary Stage

The present study was aimed to evaluate the confluence percentage of three oral cell lines, namely primary gingival keratinocytes (PGK), primary gingival fibroblasts (HGF) and tongue squamous cell carcinoma (SCC-4). All cells have been monitored at different passages for 21 days. Evaluation of confluence percentage reveals the fact that primary gingival keratinocytes and tongue squamous cell carcinoma at small passages requires a period of about two weeks to reach a confluence of approximately 80% while for the gingival fibroblasts a period of about three times smaller is satisfactory.

scholarly journals Phenotypic and molecular characterization of Acinetobacter baumannii clinical isolates in Saudi Arabia

2020 ◽  
Vol 13 (2) ◽  
pp. 327
Author(s):  
R Bawazeer ◽  
M Algoribi ◽  
T Abujamel ◽  
L Okdah ◽  
M Alzayer ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Clinical Isolates
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