scholarly journals Immunogenic Peptides from Pap31 and SCS-α of Bartonella bacilliformis: One Step Closer to a Rapid Diagnostic Tool for Carrion’s Disease

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 917
Author(s):  
Cláudia Gomes ◽  
Maria J. Pons ◽  
Juana del Valle-Mendoza ◽  
Mayumi Matsuoka ◽  
Joaquim Ruiz
Keyword(s):  
Epitope Mapping ◽  
In Silico Analysis ◽  
Limited Resources ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bartonella Bacilliformis ◽  
Immunogenic Peptides ◽  
One Step ◽  
The Common ◽  
Affected Country ◽  
Silico Analysis

Bartonella bacilliformis is the causal agent of Carrion’s disease, an overlooked illness endemic in the Andean Mountains with Peru being the most affected country. The diagnostic of this illness is a challenge due to the limited resources and the common symptomatology with other infectious diseases. The goal of this study was to identify immunogenic peptides from Pap31 and succinyl-CoA synthetase α (SCS-α) of B. bacilliformis that might be suitable for developing a serologic tool. The immunodominant character of Pap31 and SCS-α was determined by Western blotting and in-silico analysis. Subsequently, 35 peptides were selected for epitope mapping and their immunoreactivity was tested by enzyme-linked immunosorbent assay (ELISA). A total of 30 sera were tested including pre-exposed people with high IgM levels for Pap31/SCS-α (23 sera), patients (2 sera) as well as 5 sera with no reactivity to Pap31/SCS-α. The results indicate that Pap31-8 (187QAIGSAILKGTKDTGT202) and SCS-α-12 (59IFASVAEGKEKTGANA74) are the most immunogenic peptides, with Pap31-8 showing potential to discriminate between B. bacilliformis and the remaining Bartonella spp., and SCS-α-12 differentiating Bartonella spp. from other microorganisms.

scholarly journals In silico analysis of galactinol synthase genes in common bean (Phaseolus vulgaris L.)

2016 ◽  
Vol 19 (4) ◽  
pp. 45-52
Author(s):  
Bang Phi Cao
Keyword(s):  
Common Bean ◽  
In Silico Analysis ◽  
Duplication Event ◽  
Expression Level ◽  
Phylogenic Tree ◽  
Galactinol Synthase ◽  
Reproductive Tissues ◽  
The Common ◽  
First Time ◽  
Silico Analysis

Galactinol synthases (GolS, EC 2.4.1.123) belong to the glycosyltransferase 8 family. These enzymes catalyzes galactosylation of myo-inositol to form galactinol, precursor of raffinose which is solube sucrose playing an important role in stress tolerance in plants. We identified and analyzed a total of three GolS encoded genes in the whole genome of common bean. These genes possessed two or three introns. The predicted proteins contained from 322 to 340 amino acids. The common bean GolS proteins contained all conseved motifs that were characterized for other previously known GolSs. These proteins were acidic and weakly hydrophilic. The phylogenic tree constructed from GolS proteins of Arabidopsis, poplar and common bean showed that only one gene duplication event was detected in the commone bean genome. Common bean GolS genes expressed in all vegetative and reproductive tissues. However, the transcript abundances of each gene varied in different tissues. When comparing the expression levels of these three genes as defined RNAseq, we found that the expression level of PvGolS2 was the lowest, PvGolS1 expressed the strogest leaves and stem, while PvGolS3 expressed in roots and nodules. We discovered that Rhizobium incubation affected on the expression of GolS genes for the first time. In the reproductive tissues, PvGolS1 expressed in most studied tissues. Its expression level correlatively increased with the tissue development.

scholarly journals Genetic and molecular characterization of multicomponent resistance of Pseudomonas against allicin

2020 ◽  
Vol 3 (5) ◽  
pp. e202000670 ◽  
Author(s):  
Jan Borlinghaus ◽  
Anthony Bolger ◽  
Christina Schier ◽  
Alexander Vogel ◽  
Björn Usadel ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Resistance Mechanism ◽  
In Silico Analysis ◽  
Genomic Islands ◽  
Hostile Environment ◽  
Defense Substance ◽  
Individual Candidate ◽  
The Common ◽  
Silico Analysis

The common foodstuff garlic produces the potent antibiotic defense substance allicin after tissue damage. Allicin is a redox toxin that oxidizes glutathione and cellular proteins and makes garlic a highly hostile environment for non-adapted microbes. Genomic clones from a highly allicin-resistant Pseudomonas fluorescens (PfAR-1), which was isolated from garlic, conferred allicin resistance to Pseudomonas syringae and even to Escherichia coli. Resistance-conferring genes had redox-related functions and were on core fragments from three similar genomic islands identified by sequencing and in silico analysis. Transposon mutagenesis and overexpression analyses revealed the contribution of individual candidate genes to allicin resistance. Taken together, our data define a multicomponent resistance mechanism against allicin in PfAR-1, achieved through horizontal gene transfer.

Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange, and Peptide-Panning Techniques Combined with In Silico Analysis

2014 ◽  
pp. 427-446 ◽  
Author(s):  
Nicola Clementi ◽  
Nicasio Mancini ◽  
Elena Criscuolo ◽  
Francesca Cappelletti ◽  
Massimo Clementi ◽  
...  
Keyword(s):  
In Silico ◽  
Epitope Mapping ◽  
In Silico Analysis ◽  
Deuterium Exchange ◽  
Hydrogen Deuterium Exchange ◽  
Hydrogen Deuterium ◽  
Silico Analysis

scholarly journals A Novel Amino Acid Substitution, Fibrinogen Bβp.Pro234Leu, Associated with Hypofibrinogenemia Causing Impairment of Fibrinogen Assembly and Secretion

2020 ◽  
Vol 21 (24) ◽  
pp. 9422
Author(s):  
Takahiro Kaido ◽  
Masahiro Yoda ◽  
Tomu Kamijo ◽  
Shinpei Arai ◽  
Chiaki Taira ◽  
...  
Keyword(s):  
Cell Lines ◽  
In Silico ◽  
Contact Region ◽  
Chinese Hamster ◽  
In Silico Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoblotting Analysis ◽  
Fibrin Polymerization ◽  
The Impact ◽  
Silico Analysis

We identified a novel heterozygous variant, Bβp.Pro234Leu (fibrinogen Tokorozawa), which was suspected to be associated with hypofibrinogenemia. Therefore, we analyzed the assembly and secretion of this fibrinogen using Chinese hamster ovary (CHO) cells. To determine the impact on the synthesis and secretion of fibrinogen of the Bβp.P234L and γp.G242E substitutions, we established recombinant variant fibrinogen-producing CHO cell lines. Synthesis and secretion analyses were performed using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting analysis with the established cell lines. In addition, we performed fibrin polymerization using purified plasma fibrinogen and in-silico analysis. Both Bβp.P234L and γp.G242E impaired the secretion and synthesis of fibrinogen. Moreover, immunoblotting analysis elucidated the mobility migration of the Bβγ complex in Bβp.P234L. On the other hand, the fibrin polymerization of fibrinogen Tokorozawa was similar to that of normal fibrinogen. In-silico analysis revealed that the Bβp.P234 residue is located in the contact region between the Bβ and γ chains and contacts γp.G242 residue. The present study demonstrated that the Bβp.P234L substitution resulted in hypofibrinogenemia by decreasing the assembly and secretion of fibrinogen. Therefore, there is a possibility that substitutions in the contact region between the Bβ and γ chains impact the assembly and secretion of fibrinogen.

scholarly journals Geraniin Inhibits the Entry of SARS-CoV-2 by Blocking the Interaction between Spike Protein RBD and Human ACE2 Receptor

2021 ◽  
Vol 22 (16) ◽  
pp. 8604
Author(s):  
Young Soo Kim ◽  
Hwan-Suck Chung ◽  
Sang Gyun Noh ◽  
Bonggi Lee ◽  
Hae Young Chung ◽  
...  
Keyword(s):  
Viral Entry ◽  
In Silico Analysis ◽  
Spike Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Angiotensin Converting Enzyme 2 ◽  
Nephelium Lappaceum ◽  
Medical Plants ◽  
Equilibrium Dissociation ◽  
Dissociation Constant Kd ◽  
Silico Analysis

The coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Despite the development of vaccines, the emergence of SARS-CoV-2 variants and the absence of effective therapeutics demand the continual investigation of COVID-19. Natural products containing active ingredients may be good therapeutic candidates. Here, we investigated the effectiveness of geraniin, the main ingredient in medical plants Elaeocarpus sylvestris var. ellipticus and Nephelium lappaceum, for treating COVID-19. The SARS-CoV-2 spike protein binds to the human angiotensin-converting enzyme 2 (hACE2) receptor to initiate virus entry into cells; viral entry may be an important target of COVID-19 therapeutics. Geraniin was found to effectively block the binding between the SARS-CoV-2 spike protein and hACE2 receptor in competitive enzyme-linked immunosorbent assay, suggesting that geraniin might inhibit the entry of SARS-CoV-2 into human epithelial cells. Geraniin also demonstrated a high affinity to both proteins despite a relatively lower equilibrium dissociation constant (KD) for the spike protein (0.63 μM) than hACE2 receptor (1.12 μM), according to biolayer interferometry-based analysis. In silico analysis indicated geraniin’s interaction with the residues functionally important in the binding between the two proteins. Thus, geraniin is a promising therapeutic agent for COVID-19 by blocking SARS-CoV-2’s entry into human cells.

In silico analysis of Pap31 from Bartonella bacilliformis and other Bartonella spp.

2020 ◽  
Vol 84 ◽  
pp. 104482
Author(s):  
Joaquim Ruiz ◽  
Cláudia Gomes
Keyword(s):  
In Silico ◽  
In Silico Analysis ◽  
Bartonella Bacilliformis ◽  
Silico Analysis

A systematic review with in silico analysis on transcriptomic profile of gallbladder carcinoma

2020 ◽  
Vol 47 (6) ◽  
pp. 398-408
Author(s):  
Sonam Tulsyan ◽  
Showket Hussain ◽  
Balraj Mittal ◽  
Sundeep Singh Saluja ◽  
Pranay Tanwar ◽  
...  
Keyword(s):  
Systematic Review ◽  
Gallbladder Carcinoma ◽  
In Silico ◽  
In Silico Analysis ◽  
Transcriptomic Profile ◽  
Silico Analysis

In Silico Analysis of Single Nucleotide Polymorphism in Human Prothrombin Gene

2019 ◽  
Author(s):  
I. Farah ◽  
A. El-Mubark ◽  
M. Osman ◽  
A. Soliman ◽  
F. Ali ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
In Silico ◽  
In Silico Analysis ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Prothrombin Gene ◽  
Silico Analysis

A Review on Methods of Face Masks Sterilization for Reuse Owing To Crisis During COVID-19

2020 ◽  
Vol 11 (SPL1) ◽  
pp. 1240-1243
Author(s):  
Pradyuman Singh Rajput ◽  
Asish Kumar Saha ◽  
Insiya Gangardiwala ◽  
Anand Vijayakumar Palur Ramakrishnan
Keyword(s):  
Healthcare Workers ◽  
Health Hazard ◽  
Capital City ◽  
Limited Resources ◽  
Protective Equipment ◽  
First Line ◽  
Republic Of China ◽  
Enormous Amount ◽  
The Face ◽  
The Common

The COVID-19 pandemic initially started from the Wuhan capital city of Hubei Province in the People's Republic of China had now led to a severe public health hazard across the globe, the recorded death is approximately 958 thousand globally and counting. With the enormous amount of spread of the disease, a severe crisis for Personal Protective Equipment (PPE) is being noticed across the globe. Face masks being the first line of defence for all the healthcare workers as well for the common public. It became mandatory to wear face masks before entering the patient care area. The countries who are not manufacturing it locally had to depend on other countries for the procurement. As there is a severe supply chain disruption due to the lockdown measures taken by all the countries to contain the disease, so it had become difficult to procure the face masks from the manufacturing countries. The price for these PPEs is also rising at an alarming rate with the increase in the COVID-19 cases and the huge rate of consumption by the healthcare and other sectors. Therefore, with limited resources, the hospital has to run its services. The CDC, WHO and ICMR have released several guidelines from time to time for sterilization and reuse of face masks. This article will discuss the various methods that can be utilized to sterilize the face masks and reuse of it.

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