scholarly journals Synergy between “Probiotic” Carbon Quantum Dots and Ciprofloxacin in Eradicating Infectious Biofilms and Their Biosafety in Mice

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1809
Author(s):  
Yanyan Wu ◽  
Guang Yang ◽  
Henny C. van der Mei ◽  
Linqi Shi ◽  
Henk J. Busscher ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Open Space ◽  
Carbon Quantum Dots ◽  
Probiotic Bacteria ◽  
Ros Generation ◽  
E Coli ◽  
Protein Levels ◽  
Reactive Protein ◽  
Physico Chemical ◽  
Intestinal Infections

Orally administrated probiotic bacteria can aid antibiotic treatment of intestinal infections, but their arrival at their intestinal target site is hampered by killing in the gastrointestinal tract and by antibiotics solely intended for pathogen killing. Carbon-quantum-dots are extremely small nanoparticles and can be derived from different sources, including bacteria. Here, we hypothesize that carbon-quantum-dots inherit antibacterial activity from probiotic source bacteria to fulfill a similar role as live probiotics in intestinal infection therapy. Physico-chemical analyses indicated that carbon-quantum-dots, hydrothermally derived from Bifidobacterium breve (B-C-dots), inherited proteins and polysaccharides from their source-bacteria. B-C-dots disrupted biofilm matrices of Escherichia coli and Salmonella typhimurium biofilms through extensive reactive-oxygen-species (ROS)-generation, causing a decrease in volumetric bacterial-density in biofilms. Decreased bacterial densities leave more open space in biofilms and have enhanced ciprofloxacin penetration and killing potential in an E. coli biofilm pre-exposed to probiotic B-C-dots. Pathogenic carbon-quantum-dots hydrothermally derived from E. coli (E-C-dots) did not disrupt pathogenic biofilms nor enhance E. coli killing potential by ciprofloxacin. B-C-dots were biosafe in mice upon daily administration, while E-C-dots demonstrated a decrease in white blood cell and platelet counts and an increase in C-reactive protein levels. Therefore, the way is paved for employing probiotic carbon-quantum-dots instead of viable, probiotic bacteria for synergistic use with existing antibiotics in treating intestinal infections.

scholarly journals Carbon Quantum Dots Derived from Different Carbon Sources for Antibacterial Applications

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 623
Author(s):  
Yanyan Wu ◽  
Cong Li ◽  
Henny C. van der Mei ◽  
Henk J. Busscher ◽  
Yijin Ren
Keyword(s):  
Quantum Dots ◽  
Large Scale ◽  
Bacterial Resistance ◽  
Carbon Sources ◽  
Antibacterial Activities ◽  
Carbon Quantum Dots ◽  
Ros Generation ◽  
Physico Chemical ◽  
The Matrix ◽  
Natural Carbon

Nanoparticles possess unique features due to their small size and can be composed of different surface chemistries. Carbon quantum dots possess several unique physico-chemical and antibacterial activities. This review provides an overview of different methods to prepare carbon quantum dots from different carbon sources in order to provide guidelines for choosing methods and carbon sources that yield carbon quantum dots with optimal antibacterial efficacy. Antibacterial activities of carbon quantum dots predominantly involve cell wall damage and disruption of the matrix of infectious biofilms through reactive oxygen species (ROS) generation to cause dispersal of infecting pathogens that enhance their susceptibility to antibiotics. Quaternized carbon quantum dots from organic carbon sources have been found to be equally efficacious for controlling wound infection and pneumonia in rodents as antibiotics. Carbon quantum dots derived through heating of natural carbon sources can inherit properties that resemble those of the carbon sources they are derived from. This makes antibiotics, medicinal herbs and plants or probiotic bacteria ideal sources for the synthesis of antibacterial carbon quantum dots. Importantly, carbon quantum dots have been suggested to yield a lower chance of inducing bacterial resistance than antibiotics, making carbon quantum dots attractive for large scale clinical use.

scholarly journals P-Doped Carbon Quantum Dots with Antibacterial Activity

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1116
Author(s):  
Shuiqin Chai ◽  
Lijia Zhou ◽  
Shuchen Pei ◽  
Zhiyuan Zhu ◽  
Bin Chen
Keyword(s):  
Quantum Dots ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Fluorescence Emission ◽  
Carbon Quantum Dots ◽  
Microbial Pathogens ◽  
Electronic Interaction ◽  
Minimal Inhibitory Concentrations ◽  
E Coli ◽  
Zeta Potential Analysis

It is a major challenge to effectively inhibit microbial pathogens in the treatment of infectious diseases. Research on the application of nanomaterials as antibacterial agents has evidenced their great potential for the remedy of infectious disease. Among these nanomaterials, carbon quantum dots (CQDs) have attracted much attention owing to their unique optical properties and high biosafety. In this work, P-doped CQDs were prepared by simple hydrothermal treatment of m-aminophenol and phosphoric acid with fluorescence emission at 501 nm when excited at 429 nm. The P-doped CQDs showed effective antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The minimal inhibitory concentrations (MICs) of P-doped CQD were 1.23 mg/mL for E. coli and 1.44 mg/mL for S. aureus. Furthermore, the morphologies of E. coli cells were damaged and S. aureus became irregular when treated with the P-doped CQDs. The results of zeta potential analysis demonstrated that the P-doped CQDs inhibit antibacterial activity and destroy the structure of bacteria by electronic interaction. In combination, the results of this study indicate that the as-prepared P-doped CQDs can be a promising candidate for the treatment of bacterial infections.

A simple one-step hydrothermal route towards water solubilization of carbon quantum dots from soya-nuggets for imaging applications

RSC Advances ◽  
2015 ◽  
Vol 5 (106) ◽  
pp. 87528-87534 ◽  
Author(s):  
Prashant Dubey ◽  
Kumud Malika Tripathi ◽  
Ragini Mishra ◽  
Anshu Bhati ◽  
Anupriya Singh ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Hydrothermal Process ◽  
Carbon Quantum Dots ◽  
Single Step ◽  
Water Soluble ◽  
High Yield ◽  
Synthetic Approach ◽  
Hydrothermal Route ◽  
E Coli ◽  
One Step

A high yield simple synthetic approach for water soluble photoluminescent carbon quantum dots via a single step, hydrothermal process, was described. Photoluminescent multi-colored emissions were used to label E. coli cells.

scholarly journals Potential Military Cotton Textiles by Carbon Quantum Dots

2021 ◽  
Author(s):  
Hossam E. Emam ◽  
Mahmoud El-Shahat ◽  
Mohamed S. Hasanin ◽  
Hanan B. Ahmed
Keyword(s):  
Staphylococcus Aureus ◽  
Quantum Dots ◽  
Carbon Quantum Dots ◽  
Cotton Fabrics ◽  
Protection Factor ◽  
Yellowness Index ◽  
E Coli ◽  
Microbial Inhibition ◽  
Blocking Activity ◽  
Fluorescence Peak

Abstract Owing to the sensitivity for color vicissitude by exposing to UV irradiation, manufacturing of fluorescent fabrics is widely demanded to be exploited in camping, sensing and military purposes. Pyrimidine based heterocycles were investigated with excellent pharmacological activity, however, their photoluminescence activity was never been investigated till now. The presented approach demonstrate a quite novel route for manufacturing of potential military textiles (fluorescent/UV-protective cotton fabrics with micobicide activity) via exploitation of carbon quantum dots (CQDs) nucleated from pyrimidine based heterocycle (4-(2,4-dichlorophenyl)-6-oxo-2-thioxohexahydropyrimidine-5-carbonitrile, Target Molecule, TM). The synthesized TM & CQDs were separately immobilized within both of native and cationized cotton fabrics to obtain TM@cotton, CQDs@cotton, TM@Q-cotton and CQDs@Q-cotton fabrics. The estimated yellowness index, intensity of the fluorescence peak, UV-blocking activity and microbicide action, were all followed the order of CQDs@Q-cotton > TM@Q-cotton > CQDs@cotton > TM @cotton. CQDs@Q-cotton showed quite good durability, as after 5 washings, yellowness index was diminished from 26.5 to only 20.3, florescence intensity for CQDs@Q-cotton was decreased from 540 nm to 340 nm and transmission percent was increased from 7 % to 10 %. Moreover, even after 10 washings, microbial inhibition (as a percent) against E. coli, Staphylococcus aureus and Candida albicans was estimated to 63 %, 68 % and 67 %, respectively, while, UV protection factor (UPF) was diminished from 38.2 (very good) to 21.5 (good). The presented unique route was succeeded for manufacturing of durable fluorescent textiles that could be superiorly applied as potential military textiles.

Positive correlation between neovascularization degree of carotid atherosclerosis determined by contrast-enhanced ultrasound and level of serum C-reactive protein

VASA ◽  
2015 ◽  
Vol 44 (3) ◽  
pp. 0187-0194 ◽  
Author(s):  
Xiaoni Chang ◽  
Jun Feng ◽  
Litao Ruan ◽  
Jing Shang ◽  
Yanqiu Yang ◽  
...  
Keyword(s):  
Contrast Enhancement ◽  
Rank Correlation ◽  
Artery Stenosis ◽  
Contrast Enhanced Ultrasound ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Contrast Enhanced ◽  
Grade 3 ◽  
Axis View

Background: Neovascularization is one of the most important risk factors for unstable plaque. This study was designed to correlate plaque thickness, artery stenosis and levels of serum C-reactive protein with the degree of intraplaque enhancement determined by contrast-enhanced ultrasound. Patients and methods: Contrast-enhanced ultrasound was performed on 72 carotid atherosclerotic plaques in 48 patients. Contrast enhancement within the plaque was categorized as grade 1, 2 or 3. Maximum plaque thickness was measured in short-axis view. Carotid artery stenosis was categorized as mild, moderate or severe. Results: Plaque contrast enhancement was not associated with the degree of artery stenosis or with plaque thickness. Serum C-reactive protein levels were positively correlated with the number of new vessels in the plaque. C-reactive protein levels increased in the three groups(Grade 1: 3.72±1.79mg/L; Grade 2: 7.88±4.24 mg/L; Grade 3: 11.02±3.52 mg/L), with significant differences among them (F=10.14, P<0.01), and significant differences between each two groups (P<0.05). Spearman’s rank correlation analysis showed that serum C-reactive protein levels were positively correlated with the degree of carotid plaque enhancement (Rs =0.69, P<0.01). Conclusions: The combination of C-reactive protein levels and intraplaque neovascularization detected by contrast-enhanced ultrasound may allow more accurate evaluation of plaque stability.

Sign in / Sign up

Export Citation Format

Share Document