scholarly journals Polymeric Materials for Hemostatic Wound Healing

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2127
Author(s):  
Suvash Ghimire ◽  
Pritha Sarkar ◽  
Kasey Rigby ◽  
Aditya Maan ◽  
Santanu Mukherjee ◽  
...  
Keyword(s):  
Wound Healing ◽  
Visual Inspection ◽  
First Responders ◽  
Polymeric Materials ◽  
Severe Bleeding ◽  
Military Operations ◽  
Manual Pressure ◽  
External Wound ◽  
Wounded Area

Hemorrhage is one of the greatest threats to life on the battlefield, accounting for 50% of total deaths. Nearly 86% of combat deaths occur within the first 30 min after wounding. While external wound injuries can be treated mostly using visual inspection, abdominal or internal hemorrhages are more challenging to treat with regular hemostatic dressings because of deep wounds and points of injury that cannot be located properly. The need to treat trauma wounds from limbs, abdomen, liver, stomach, colon, spleen, arterial, venous, and/or parenchymal hemorrhage accompanied by severe bleeding requires an immediate solution that the first responders can apply to reduce rapid exsanguinations from external wounds, including in military operations. This necessitates the development of a unique, easy-to-use, FDA-approved hemostatic treatment that can deliver the agent in less than 30 s and stop bleeding within the first 1 to 2 min at the point of injury without application of manual pressure on the wounded area.

scholarly journals Factor XIII-A: An Indispensable “Factor” in Haemostasis and Wound Healing

2021 ◽  
Vol 22 (6) ◽  
pp. 3055
Author(s):  
Fahad S. M. Alshehri ◽  
Claire S. Whyte ◽  
Nicola J. Mutch
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Crucial Role ◽  
Factor Xiii ◽  
Severe Bleeding ◽  
Bleeding Diathesis ◽  
Unknown Mechanism ◽  
Protein Substrates ◽  
Physiological Processes ◽  
Isopeptide Bonds

Factor XIII (FXIII) is a transglutaminase enzyme that catalyses the formation of ε-(γ-glutamyl)lysyl isopeptide bonds into protein substrates. The plasma form, FXIIIA2B2, has an established function in haemostasis, with fibrin being its principal substrate. A deficiency in FXIII manifests as a severe bleeding diathesis emphasising its crucial role in this pathway. The FXIII-A gene (F13A1) is expressed in cells of bone marrow and mesenchymal lineage. The cellular form, a homodimer of the A subunits denoted FXIII-A, was perceived to remain intracellular, due to the lack of a classical signal peptide for its release. It is now apparent that FXIII-A can be externalised from cells, by an as yet unknown mechanism. Thus, three pools of FXIII-A exist within the circulation: plasma where it circulates in complex with the inhibitory FXIII-B subunits, and the cellular form encased within platelets and monocytes/macrophages. The abundance of this transglutaminase in different forms and locations in the vasculature reflect the complex and crucial roles of this enzyme in physiological processes. Herein, we examine the significance of these pools of FXIII-A in different settings and the evidence to date to support their function in haemostasis and wound healing.

scholarly journals Management of Chronic Non-Healing Wound using Ayurvedic drugs - A Case Study

2020 ◽  
Vol 11 (2) ◽  
pp. 337-341
Author(s):  
Hemanta Kumar Panigrahi
Keyword(s):  
Wound Healing ◽  
Subcutaneous Tissue ◽  
Oral Intake ◽  
Central Research Institute ◽  
Central Research ◽  
Ayurvedic Drugs ◽  
Superficial Wound ◽  
Healing Wound ◽  
Skin Loss ◽  
External Wound

Sushruta in 1st part of his compendium described about wound healing its types etc. as Vranitopasaniya adhyaya. Ayurveda described so many compound medicines and oil for oral administration and topical application to accelerate the wound healing. But this is not scientifically validated. So there is always a search for finding a newer and better medicine for wound healing. I am presenting a case of 42 year old married male with complain of superficial external wound with mild pain,  mal odour  and not healing  since 45 days in spite of taking allopathic conventional treatment in his lower limb. The study was done in my outpatient department of Central Research institute for cardiovascular disease, Punjabi Bagh, New Delhi. On examination it was found that surface area of superficial wound is 22.5 sq. cm. having full thickness, skin loss involving necrosis of subcutaneous tissue extended down to underlying fascia. Necessary laboratory investigations were done prior to initiate the treatment. The wound was irrigated with Panchvalkala kasaya and dressing with Jatyadi Tail was done daily followed by oral intake of Triphala guggulu 500mg twice in a day and Amalaki churna 6gm twice in a day was administered orally for 28th days. Periodic follow up was done on 0, 7, 14, and in 28 days. This regimen was found to be effective in faster wound epithelialization, and reducing wound exudates. There was no evidence of any allergic reaction as well as no evidence showing any adverse event on the wound.

Studies on Wound Healing: Effects of Calcium D-Pantothenate on the Migration, Proliferation and Protein Synthesis of Human Dermal Fibroblasts in Culture

1999 ◽  
Vol 69 (2) ◽  
pp. 113-119 ◽  
Author(s):  
Weimann ◽  
Hermann
Keyword(s):  
Wound Healing ◽  
Protein Synthesis ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Wound Healing Process ◽  
Human Dermal Fibroblasts ◽  
The Mean ◽  
Cell Densities ◽  
Migration Of Cells ◽  
Wounded Area

The effect of calcium D-pantothenate on the migration, proliferation and protein synthesis of human dermal fibroblasts from three different donors was investigated. The migration of cells into a wounded area was dose-dependently stimulated by Ca D-pantothenate. The number of cells that migrated across the edge of the wound increased from 32 ± 7 cells/ mm without Ca D-pantothenate to 76 ± 2 cells/ mm with 100 mg/ml Ca D-pantothenate. Moreover, the mean migration distance per cell increased from 0.23 ± 0.05 mm to 0.33 ± 0.02 mm. The mean migration speed was calculated to be 10.5 mm/hour without and 15 mm/hour with Ca D-pantothenate. Cell proliferation was also dose-dependently stimulated. The final cell densities were 1.2 to 1.6-fold higher in cultures containing 100 mg/ml Ca D-pantothenate. The protein synthesis was modulated, since two unidentified proteins were more strongly expressed in pantothenate supplemented cultures. In conclusion, Ca D-pantothenate accelerates the wound healing process by increasing the number of migrating cells, their distance and hence their speed. In addition, cell division is increased and the protein synthesis changed. These results suggest that higher quantities of pantothenate are locally required to enhance wound healing.

Hemostasis and Thrombosis in Trauma Patients

2015 ◽  
Vol 41 (01) ◽  
pp. 026-034 ◽  
Author(s):  
Satoshi Gando
Keyword(s):  
Wound Healing ◽  
Protein C ◽  
Activated Protein C ◽  
Fat Embolism ◽  
Whole Body ◽  
Severe Bleeding ◽  
Trauma Patients ◽  
Embolism Syndrome ◽  
Acute Coagulopathy Of Trauma ◽  
Pathological Reaction

Hemostasis and thrombosis in trauma patients consist of physiological hemostasis for wound healing and the pathological reaction of disseminated intravascular coagulation (DIC). Whole body trauma, isolated brain injury, and fat embolism syndrome, if extremely severe, can cause DIC and affect a patient's prognosis. Shock-induced hyperfibrinolysis causes DIC with the fibrinolytic phenotype, contributing to oozing-type severe bleeding. If uncontrolled, this phenotype progresses to thrombotic phenotype at the late stage of trauma, followed by microvascular thrombosis, leading to organ dysfunction. Another type of pathological hemostatic change is acute coagulopathy of trauma shock (ACOTS), which gives rise to activated protein C–mediated systemic hypocoagulation, resulting in bleeding. ACOTS occurs only in trauma associated with shock-induced hypoperfusion and there is nothing to suggest DIC in this phenomenon. This review will provide information about the recent advances in hemostasis and thrombosis in trauma and will clarify the pathogeneses of the pathological processes observed in trauma patients.

Development and characterization of silk films for burn wound healing

2020 ◽  
Vol 24 (2) ◽  
pp. 131-146
Author(s):  
Sukumar Nachiappan ◽  
Lami Amanuel ◽  
Tewodros Agazie ◽  
Seyoum Bihonegn
Keyword(s):  
Wound Healing ◽  
Research Work ◽  
Polymeric Materials ◽  
Study Data ◽  
Burn Wound ◽  
Content Type ◽  
Burn Wound Healing ◽  
Biomedical Device ◽  
Silk Films

Purpose Wound healing is a dynamic process that relies on coordinated signaling molecules to succeed. Silk has proven to be a promising biomaterial for the development of a novel product. The purpose of the study is development of silk films, augmented functionality can be provided to silk by means of loading honey and recombinant human epidermal growth factor (rhEGF). Design/methodology/approach In this research work, the authors set out to explore possibilities of silk-based biomedical device development with particular attention to different fabrication strategies that can be leveraged for this purpose. They have produced a novel silk-based drug delivery material, in the form of silk films. Scanning electronic microscope was used to observe the morphology and the highly specific surface area. The structure was studied by Fourier-transform infrared spectroscopy. This methodology is accomplished using in vivo study data using Wister albonia rats. Findings The developed films also provided a significant higher healing rate in vivo, with well-formed epidermis with faster granulation tissue formation when compared to the controls. Biodegradable polymeric materials based on blending aqueous dispersions of natural polymer sodium alginate, Chitosan and rhEGF complex, which allow controlled antiseptic release, are presented. Originality/value These results suggest that silk-based controlled release of Chitosan-rhEGF may serve as a new therapy to accelerate healing of burn wounds.

Plasmin/plasminogen is essential for the healing of tympanic membrane perforations

2006 ◽  
Vol 96 (10) ◽  
pp. 512-519 ◽  
Author(s):  
Jinan Li ◽  
Per-Olof Eriksson ◽  
Annika Hansson ◽  
Sten Hellström ◽  
Tor Ny
Keyword(s):  
Wound Healing ◽  
Tympanic Membrane ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Keratinocyte Migration ◽  
Tympanic Membrane Perforations ◽  
Wounded Area ◽  
Deficient Mice

SummaryPlasminogen has been proposed to play an important role in different tissue remodeling processes such as wound healing and tissue regeneration after injuries. The healing of tympanic membrane perforations is a well-organized chain of inflammatory events, with an initial invasion of inflammatory cells followed by reparative and restoration phases. Here we show that the healing of tympanic membrane perforations is completely arrested in plasminogen-deficient mice, with no signs of any healing even 143 days after perforation. Inflammatory cells were recruited to the wounded area, but there were no signs of tissue debridement. In addition, removal of fibrin, keratinocyte migration and in-growth of connective tissue were impaired. This contrasts with skin wound healing, where studies have shown that, although the healing process is delayed, it reaches completion in all plasminogen-deficient mice. Our finding that keratinocyte migration and re-epithelialization were completely arrested in plasminogen-deficient mice indicates that plasminogen/plasmin plays a more profound role in the healing of tympanic membrane perforations than in the healing of other epithelial wounds.

scholarly journals ROSELLE CALYX ETHANOL EXTRACT STIMULATES ORAL MUCOSAL WOUND HEALING

2018 ◽  
Vol 9 ◽  
pp. 36
Author(s):  
Sarwono At ◽  
Suniarti Df
Keyword(s):  
Wound Healing ◽  
Ethanol Extract ◽  
Lymphocytic Infiltration ◽  
Punch Biopsy ◽  
Sprague Dawley ◽  
Standard Temperature ◽  
Sprague Dawley Rats ◽  
Similar Formation ◽  
Wounded Area ◽  
Mucosal Wound

Objective: This study aim to analyze the effect of the roselle calyx ethanol extract on oral mucosal wound healing.Methods: Male Sprague Dawley rats (3 month, 150 g) were housed under standard temperature conditions (20–22°C). Punch biopsy wounds were inflicted on the inferior labial mucosal membrane of rats. All treatments (normal saline; 0.1% triamcinolone acetonide; and 3.75%, 7.5%, and 15% roselle calyx ethanol extract) were applied 2 times daily. Wounded areas were observed after 8 days of treatment. The specimen of the wounded area was prepared and microscopic slides were analyzed under a light microscope.Results: The histological features of the groups treated with TA and 7.5% roselle calyx ethanol extract showed a similar formation of the epidermis layer and no lymphocytic infiltration of the lamina propria.Conclusion: The application of 7.5% roselle calyx ethanol extract could stimulate oral mucosal wound healing.

Problematic External Wound Healing after ACL-Reconstructive Surgery

2002 ◽  
Vol 7 (3) ◽  
pp. 36-37
Author(s):  
Joseph J. Piccininni ◽  
Malissa Martin ◽  
Sheila Curry
Keyword(s):  
Wound Healing ◽  
Reconstructive Surgery ◽  
External Wound

Accelerated aging investigation of high voltage EPDM/silica composite insulators

2016 ◽  
Vol 36 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Abraiz Khattak ◽  
Muhammad Amin
Keyword(s):  
Electron Microscopy ◽  
Hybrid Composite ◽  
Visual Inspection ◽  
Accelerated Aging ◽  
Polymeric Materials ◽  
Ethylene Propylene Diene Monomer ◽  
Radiation Heat ◽  
Insulating Materials ◽  
Salt Fog ◽  
Scanning Electron

Abstract Ethylene-propylene-diene-monomer (EPDM) is one of the most frequently used outdoor insulating materials. Like other polymeric materials, EPDM and its composites also degrade in outdoor applications. For evaluation of aging of insulating materials, accelerated multistress aging/weathering is an efficient method. In the current paper, comparative multistress aging performance investigation of neat EPDM (NE), EPDM with 20% microsilica (microcomposite, EMC), EPDM with 5% nanosilica (nanocomposite, ENC) and EPDM 20% microsilica 5% nanosilica (hybrid composite, EHC) is presented. Materials prepared according to ASTM D3182-07 were subjected to uniform ultraviolet (UV) radiation, heat, humidity, salt fog and acid rain at 2.5 kV voltage for 5000 h in a specially fabricated weathering chamber. For timely analysis of the aged materials, Fourier transform infrared (FTIR) spectroscopy, Swedish Transmission Research Institute (STRI) hydrophobicity classification and scanning electron microscopy (SEM) were used along with the critical visual inspection. Increasing discoloration was found in all materials which was proportional to% wt of the filler in composites. Improved hydrophobicity and better surface smoothness was recorded in composites as compared to NE, which was higher in the case of the microcomposite and hybrid composite. FTIR results showed least reduction in hydrocarbon bonds and lowest variation in chalking index in the case of the microcomposite.

scholarly journals A Case of a Patient Who Successfully Achieved Early Wound Closure by Local Negative Pressure Wound Therapy (NPWT) against Compromised Wound Healing after Arterio-Venous Graft Infection

2021 ◽  
Vol 1 (1) ◽  
pp. 14-19
Author(s):  
Risa Kusuta ◽  
Keiji Shimazu ◽  
Kazuhiro Mizoguchi ◽  
Kazumasa Komura ◽  
Atsuo Tanaka
Keyword(s):  
Wound Healing ◽  
Negative Pressure ◽  
Wound Closure ◽  
Negative Pressure Wound Therapy ◽  
Present Report ◽  
Graft Infection ◽  
Severe Bleeding ◽  
Wound Therapy ◽  
Venous Graft ◽  
Early Wound

The primary treatment strategy for arterio-venous graft (AVG) infection includes appropriate antibiotic use and removal of the infected graft. It is well known that patients with hemodialysis are likely to experience compromised wound healing, which often leads to various postoperative complications. Negative pressure wound therapy (NPWT) is a non-invasive procedure that promotes wound healing by sealing the wound under negative pressure. Although NPWT is practically accepted in general surgery, there are only a few reports of this strategy to the vascular access operation for patients with hemodialysis due to the possibility of severe bleeding. In the present report, we report a case of a patient who successfully achieved safe and early wound closure by NPWT against compromised wound healing after AVG infection.

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