scholarly journals Efficient Protocol for Improving the Development of Cryopreserved Embryonic Axes of Chestnut (Castanea sativa Mill.) by Encapsulation–Vitrification

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 231
Author(s):  
Mariam Gaidamashvili ◽  
Eka Khurtsidze ◽  
Tamari Kutchava ◽  
Maurizio Lambardi ◽  
Carla Benelli
Keyword(s):  
Activated Charcoal ◽  
Castanea Sativa ◽  
Alginate Beads ◽  
Embryonic Axes ◽  
Root Regrowth ◽  
Castanea Sativa Mill ◽  
Whole Plants ◽  
Long Term Preservation ◽  
Direct Immersion

An optimized cryopreservation protocol for embryonic axes (EAs) of chestnut (Castanea sativa Mill.) has been developed based on the encapsulation–vitrification procedure. EAs of mature seeds were aseptically dissected and encapsulated in alginate beads with or without 0.3% (w/v) activated charcoal (AC). Embedded EAs were dehydrated with Plant Vitrification Solution 2 for different treatment times up to 120 min, followed by direct immersion in liquid nitrogen. Cryopreserved embryonic axes encapsulated with AC showed higher survival (70%) compared to those encapsulated without AC (50%). Sixty-four percent of embryonic axes, from synthetic seeds with AC, subsequently developed as whole plants. Plantlet regrowth was faster in AC-encapsulated EAs and showed enhanced postcryopreservation shoot and root regrowth over 2 cm after five weeks from rewarming. Results indicate that encapsulation–vitrification with activated charcoal added to the beads is an effective method for the long-term preservation of Castaneasativa embryonic axes.

scholarly journals Development of an Efficient ‘One-Step Freezing’ Cryopreservation Protocol for a Georgian Provenance of Chestnut (Castanea sativa Mill.) Zygotic Embryos

2019 ◽  
Vol 47 (4) ◽  
pp. 1047-1054
Author(s):  
Mariam GAIDAMASHVILI ◽  
Eka KHURTSIDZE ◽  
Carla BENELLI ◽  
Maurizio LAMBARDI
Keyword(s):  
Castanea Sativa ◽  
Digital Object Identifier ◽  
Zygotic Embryos ◽  
Embryonic Axes ◽  
Fast Evaluation ◽  
Potted Plants ◽  
Castanea Sativa Mill ◽  
One Step ◽  
Vitrification Solution ◽  
Pre Treatment

Experiments were performed to determine the influence of various dehydration and vitrification treatment times on the ‘one-step freezing’ cryopreservation of embryonic axes (EAs), composed of zygotic embryos and cotyledon residuals, from mature seeds of a Georgian provenance of chestnut (Castanea sativa Mill.). Dehydration was carried out in laminar flow hood from 1 to 5 h, and vitrification experiments were carried out by immersion of EAs in PVS2 vitrification solution up to 120 min, both followed by direct immersion in liquid nitrogen. Both systems resulted in inducing specimen tolerance to ultra-rapid freezing, although to a different extent. Full germination of cryo-stored EAs after 5 h of dehydration (reducing moisture content from initial 66% to 21%) has been increased from 0% to 66.7%. A pre-treatment of EAs in PVS2 vitrification solution for 30 min produced fully developed plantlets at a rate of 55.6% in post-cryopreservation. Plantlet regrowth from cryopreservation was faster in EAs that underwent the dehydration/‘one-step freezing’ procedure. All the plantlet from cryopreserved EAs could be easily acclimatized, producing healthy potted plants. Finally, the TTC test showed to be useful for a fast evaluation of specimen survival after thawing and, as a consequence, to speed up the development of optimized cryo-protocols.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Development of Probiotic Lactobacillus sporogenes Loaded Biological Macromolecules using Novel Assembly Design for Long-term Preservation as Bio-therapeutic Agent

2021 ◽  
Vol 14 (3) ◽  
pp. 5481-5491
Author(s):  
Himanshu Solanki ◽  
Madhavi P. Ghumare ◽  
Vipul D Prajapati ◽  
Sanjeev R Acharya
Keyword(s):  
Encapsulation Efficiency ◽  
Alginate Beads ◽  
Biological Macromolecules ◽  
Automated Production ◽  
Assembly Design ◽  
Intestinal Delivery ◽  
Structure Surface ◽  
Long Term Preservation

The goal present investigation was to formulate and characterized of biological macromolecules of alginate (ALG) and carboxymethylcellulose sodium (CMC) containing probiotic bacteria of Lactobacillus sporogenes (LS) co-encapsulated with a prebiotics, Bioecolians (α-Gluco-oligosaccharides). The prepared beads were characterized in terms of yield, size, encapsulation efficiency, viabilities in simulated gastric (pH 1.2, 2 hours) and bile (1% w/v, 3 hours) conditions. The beads were also characterized by FTIR, DSC, SEM and XRD to investigate molecular structure, surface properties and morphology of beads. The results showed that spherical beads with size distribution ranging from 1.18 ± 0.11 to 1.45 ± 0.15 mm for ALG and from 1.3 ± 0.12 to 1.5 ± 0.16 mm for ALG-CMC with encapsulation efficiency higher than 90% were achieved. The results indicated that incorporation of carboxymethylcellulose sodium into alginate beads improved viability of the bacteria in simulated gastric conditions as well as bile conditions. According to our in vitro studies, Probiotic beads using combination of ALG-CMC are suitable encapsulating polymer for gastro-intestinal delivery as designed by novel assembly using peristaltic pump for automated production.

scholarly journals ANALYSIS OF ECOLOGICAL AND BIOLOGICAL FEATURES OF CASTANEA SATIVA MILL. IN NATURAL AND CLIMATE CONDITIONS OF THE CENTRAL BLACK EARTH REGION (ON THE EXAMPLE OF THE VORONEZH REGION)

2021 ◽  
Vol 11 (1) ◽  
pp. 24-35
Author(s):  
Svetlana Degtyareva ◽  
Valentina Dorofeeva ◽  
Valentina Shipilova
Keyword(s):  
Castanea Sativa ◽  
Cold Weather ◽  
Climate Conditions ◽  
Biological Features ◽  
Factors Influencing ◽  
Voronezh Region ◽  
Castanea Sativa Mill ◽  
The Stability ◽  
Species Ecology

Knowledge of species ecology and biology in general and characteristics of its acclimatization in a particular region are the basis for its successful selection. In this work, the authors present the results of long-term phenological observations of Castanea sativa Mill. since its planting (1967). Ecological and biological analysis has showed some discrepancies in the passage of phenophases. If the growth processes were not completed before the onset of the autumn-winter cold weather, then in recent years the leaf fall ends at the end of October, annual shoots lignify, growth and generative buds are formed. But, in general, it allows us to draw conclusions about the correspondence of the seasonal rhythms of development of the studied sowing chestnut as an introduced species to their local ecological-phytocenotic analogues. It was experimentally established that the fruits of the chestnut do not need pre-sowing treatment, because seed germination is high (70%). The groups of leading factors influencing the stability and development of the species as a whole (in the study area) have been identified. These are: lack of heat in summer, which affects the formation of fruits; short autumn period with high temperature drops, which limits the ripening of the shoots; different spring periods, contributing to the loss of hardening; unstable winter with sharp cold snaps and thaws, intensifying the nature of winter damage

scholarly journals Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification

2017 ◽  
Vol 66 (1-2) ◽  
pp. 44-50
Author(s):  
Tatjana Vujović ◽  
Đurđina Ružić ◽  
Radosav Cerović
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Shoot Tips ◽  
Lower Survival Rate ◽  
Droplet Vitrification ◽  
Vitrification Solution ◽  
Long Term Preservation ◽  
Direct Immersion

SummaryIn vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40 min at room temperature with the PVS3 solution (glycerol 50% and sucrose 50%). They were subsequently frozen in individual microdroplets of vitrification solution, by direct immersion in liquid nitrogen (LN), and kept therein for 2, 4, 8 and 24 h. The explant rewarming was performed in an unloading solution (0.8 M sucrose) for 30 min at room temperature. The duration of LN exposure did not exert significant effects on the survival and regrowth of explants in both types of vitrification solutions. The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively. However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants. Monitoring the shoots regenerated in the in vitro culture revealed their normal capacity for multiplication and rooting in comparison with the controls, which fully confirms the purpose of cryopreservation in the long-term preservation of plant material.

scholarly journals Long-term oral administration of sweet chestnut (Castanea sativa Mill.) extract does not affect the contraction ability of isolated ileum

2019 ◽  
Vol 88 (2) ◽  
pp. 219-223
Author(s):  
Andrej Javornik ◽  
Kaja Blažič ◽  
Nika Šehić ◽  
Tomaž Snoj
Keyword(s):  
Castanea Sativa ◽  
Term Treatment ◽  
Farm Animals ◽  
Feed Additive ◽  
Control Group ◽  
Organ Bath ◽  
Sweet Chestnut ◽  
Long Term Treatment ◽  
Castanea Sativa Mill

Extracts of sweet chestnut (Castanea sativa Mill.) which contain hydrolysable tannins are used to prevent diarrhoea in farm animals due to the astringent effect of tannins. The aim of this study was to establish whether long-term treatment with the chestnut extract as a feed additive affects the acetylcholine (ACh)-triggered contractions of isolated rat ileum. Wistar rats of both sexes were divided into 4 male and 4 female groups with 6 animals in each group. The first, second and third groups of both sexes received feed with 3, 15 and 30 g of chestnut extract per kg, respectively. The fourth group of both sexes received feed without the added chestnut extract and served as the control group. After the 90-day trial, the animals were euthanized, and ilea were isolated and placed in an organ bath filled with Tyrode’s solution. Ilea were connected to an isometric transducer. Ileal contractions were triggered by 0.005, 0.01, 0.05, 0.1, 0.5, 1.0, 5.0 and 10.0 mg of ACh, and contractions were recorded. The groups exhibited dose responsiveness to the ACh treatment steps; however, no significant differences in the contraction intensity were observed among the groups at the same ACh dose level. These results suggest that long-term treatment with chestnut extract as an astringent does not influence the intensity of ileal contractions triggered by ACh. Thus, no adverse effect on intestinal motility can be expected during a long-term use of chestnut extract as a feed additive in livestock production; however, further studies in target animal species are needed to confirm the findings.

scholarly journals Sweet Chestnut (Castanea sativa Mill.) in Britain: Re-assessment of its Status as a Roman Archaeophyte

Britannia ◽  
2019 ◽  
Vol 50 ◽  
pp. 49-74 ◽  
Author(s):  
R. Jarman ◽  
Z. Hazell ◽  
G. Campbell ◽  
J. Webb ◽  
F.M. Chambers
Keyword(s):  
Plant Material ◽  
Castanea Sativa ◽  
Profound Impact ◽  
Sweet Chestnut ◽  
Castanea Sativa Mill ◽  
Supplementary Material ◽  
Archived Specimens ◽  
Archaeological Excavations ◽  
Chestnut Wood

ABSTRACTThe Roman period sees the introduction of many new plants and animals into Britain, with a profound impact on people's experience of their environment. Sweet chestnut is considered to be one such introduction, for which records of sweet chestnut wood and charcoal from archaeological excavations of Romano-British period contexts have been used as evidence. This paper reviews the records for sweet chestnut in Britain pre-a.d. 650, by critically evaluating original excavation reports and examining archived specimens. This review re-assesses the original identifications of sweet chestnut and/or their dating and concludes that most of the evidence that justified sweet chestnut's status as a Roman archaeophyte is untenable. The review emphasises the importance of securely identifying and directly dating plant material and of long-term curation by museums and archives. The Supplementary Material online (https://doi.org/10.1017/S0068113X19000011) contains details of all published records of finds of sweet chesnut.

Problems of treatment of primary openangle glaucoma in combination with thrombosis of the central retinal vein

GlaucomaNews ◽  
2020 ◽  
pp. 65-69
Author(s):  
T.E. Lipatkina ◽  
◽  
Е.V. Karlova ◽  
A.V. Zolotarev ◽  
◽  
...  
Keyword(s):  
Macular Edema ◽  
Primary Open Angle Glaucoma ◽  
Antihypertensive Drugs ◽  
Open Angle Glaucoma ◽  
Vascular Wall ◽  
Open Angle ◽  
Retinal Edema ◽  
Long Term Preservation ◽  
Central Retinal Vein

Patients with primary open-angle glaucoma (POAG) and ophthalmic hypertension have an increased likelihood of developing occlusions (thrombosis) of the central retinal vein. Different groups of antihypertensive drugs differ in their mechanism of action and may affect concomitant ocular pathology, in particular, retinal edema, which occurs, for example, in occlusion of the central retinal vein. Used in most patients with glaucoma, prostaglandin analogs can contribute to the long-term preservation of macular edema due to the effect on the permeability of the vascular wall. Preparations of other pharmacological groups, reducing the production of aqueous humor, on the contrary, may contribute to its regression. Therefore, the question of choosing a drug for antihypertensive therapy in patients with primary open-angle glaucoma and concomitant macular edema is relevant and is for further study.

Sign in / Sign up

Export Citation Format

Share Document