scholarly journals Phytochemical Profiling of Methanolic Fruit Extract of Gardenia latifolia Ait. by LC-MS/MS Analysis and Evaluation of Its Antioxidant and Antimicrobial Activity

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 545
Author(s):  
Y. Mohan Reddy ◽  
S. P. Jeevan Kumar ◽  
K. V. Saritha ◽  
P. Gopal ◽  
T. Madhusudana Reddy ◽  
...  
Keyword(s):  
Quinic Acid ◽  
Differential Pulse ◽  
Deciduous Tree ◽  
Food Industries ◽  
Analysis And Evaluation ◽  
Southern States ◽  
Ms Analysis ◽  
Antioxidant And Antimicrobial Activity ◽  
Microtiter Assay ◽  
Caffeoyl Quinic Acid

Gardenia latifolia Ait. (Rubiaceae) is also known as Indian Boxwood is a small deciduous tree often growing in southern states of India. In the present study, phytochemical profiling of methanolic extract of G. latifolia fruits were carried out using FTIR and LC-MS/MS analysis. Besides, its antioxidant and antimicrobial potential have been analysed using DPPH activity, differential pulse voltammetry and resazurin microtiter assay, respectively. Phytochemical profiling revealed the presence of 22 major diversified compounds and main were 3-caffeoyl quinic acid (chlorogenic acid), 3,4-Di-O-caffeoyl quinic acid, 6-O-trans-feruloylgenipin gentiobioside, 10-(6-O-trans sinapoyl glucopyranosyl) gardendiol, isoquercitrin, scortechinones, secaubryenol, iridoids and quercetin 3-rutinoside (rutin). The extract showed antioxidant activity (IC50 = 65.82) and powerful antibacterial activity with lowest minimum inhibitory concentration against Gram-positive Staphylococcus aureus (15.62 µg/µL), Bacillus subtilis (31.25 µg/µL) than gram negative Escherichia coli (62.5 µg/µL), Klebsiella pneumoniae (62.5 µg/µL), Pseudomonas aeruginosa (31.25 µg/µL). This study shows that the fruits of G. latifolia have tremendous potential to be used in food industries, phyto-therapeutics and cosmetic industries.

scholarly journals PHYTOCHEMICAL EVALUATION OF TILIACORA RACEMOSA COLEBR. USING GAS CHROMATOGRAPHY - MASS SPECTROMETRY (GC-MS)

2018 ◽  
Vol 11 (2) ◽  
pp. 350
Author(s):  
Yogeshwari C ◽  
Kumudha P
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Quinic Acid ◽  
Physicochemical Analysis ◽  
Drug Formulation ◽  
Gas Chromatography Mass Spectrometry ◽  
Isolation And Characterization ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

 Objective:The objective of this study is to characterize the phytoconstituents of Tiliacora racemosa Colebr. using gas chromatography mass spectrometry (GC-MS).Methods: Preliminary phytochemical and physicochemical analysis was carried out using standard procedures. GC-MS analysis of methanolic extract was carried out using Thermo GC-Trace Ultra version: 5.0, Thermo MS DSQ with a DB 35MS capillary standard non-polar column and gas chromatograph interfaced to a mass selective detector (MS DSQ II) with Xcalibur software.Results: Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, tannins, triterpenoids, steroids, proteins and amino acids, carbohydrates, saponins and coumarin. Quinones, anthraquinones, glycosides and fixed oil were absent. GC-MS analysis revealed the presence of 28 compounds of which quinic acid (retention times [RT]: 15.65) and inositol, 1-deoxy-(CAS) (RT: 19.24) was observed as abundant compounds.Conclusion: The presence of various bioactive compounds confirms the medicinal importance and it’s application for curing various diseases by traditional practitioners. However, isolation and characterization of potential bioactive compounds would lead to drug formulation.

scholarly journals Aronia melanocarpa Fruit and Leaves Hot-Assisted Ethanolic Extracts Antioxidant Activity

Proceedings ◽  
2020 ◽  
Vol 57 (1) ◽  
pp. 57
Author(s):  
Andrei Munteanu ◽  
Alin Enache ◽  
Georgeta Neagu ◽  
Corina Bubueanu ◽  
Alice Grigore ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quinic Acid ◽  
Antioxidant Compounds ◽  
Total Phenols ◽  
Aronia Melanocarpa ◽  
Fresh Material ◽  
Ethanolic Extracts ◽  
Black Chokeberry ◽  
Caffeoyl Quinic Acid

Aronia melanocarpa L. fruit (common black chokeberry) is one of the most abundant sources of antioxidant compounds in the plant world, superior to all edible fruits; chokeberry fruits contain up to 100 g total phenols per kg fresh material, predominantly (−)epicatechin, cyanidin-3-glycosides and procyanidins (60%), added to quercetin and caffeoyl quinic acid derivates.

scholarly journals Simultaneous Determination of Nine Bioactive Constituents of Caulis Lonicerae Japonicae by High-Performance Liquid Chromatography Coupled with Mass Spectrometry

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Hui-Jun Li ◽  
Zheng-Ming Qian ◽  
Ping Li ◽  
Mei-Ting Ren ◽  
Jun Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Limit Of Detection ◽  
Quinic Acid ◽  
Column Temperature ◽  
Caffeoyl Quinic Acid

A new high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) method has been developed for the simultaneous determination of nine major compounds, namely chlorogenic acid (1), caffeic acid (2), sweroside (3), loganin (4), secoxyloganin (5), 3,5-di- O-caffeoyl quinic acid (6), luteolin-7- O-glucoside (7), rutin (8) and 3,4-di- O-caffeoyl quinic acid (9), in Caulis Lonicerae Japonicae (CLJ), a commonly used traditional Chinese medicinal herb. The separation was achieved on a C-18 column (250 × 4.6 mm, 5.0 μm) with a column temperature of 30°C and a flow-rate of 0.8 mL/min. The mobile phase was composed of (A) aqueous formic acid (0.1%, v/v) and (B) methanol, using a gradient elution of 30% B for 0-13 min, 30–40% B for 13–17 min, and 40–49% B for 17–30 min. The limit of detection ( S/ N = 3) ranged from 0.8 to 5.1 ng/mL and the limit of quantification ( S/ N = 10) varied from 3.4 to 16.9 ng/mL. All calibration curves showed good linear regression ( r2 > 0.9976) within the test ranges. The intra- and inter-day precisions, as determined from sample solutions, were below 2.2 and 4.3%, respectively. The recoveries for nine compounds were within 91.3 and 104.2%. This proposed method has been successfully applied to evaluation of commercial samples of CLJ from different markets in China, which provides a new basis of assessment of the quality of the herbal drug.

Enzymatic synthesis of fatty esters in 5-caffeoyl quinic acid

2000 ◽  
Vol 102 (2) ◽  
pp. 93-95 ◽  
Author(s):  
Bernard Guyot ◽  
Dominique Gueule ◽  
Michel Pina ◽  
Jean Graille ◽  
Vicent Farines ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Quinic Acid ◽  
Fatty Esters ◽  
Caffeoyl Quinic Acid

scholarly journals GC-MS Analysis and Hemolytic, Antipyretic and Antidiarrheal Potential of Syzygium aromaticum (Clove) Essential Oil

Separations ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 35
Author(s):  
Cristiane Marinho Uchôa Lopes ◽  
João Ricardhis Saturnino de Oliveira ◽  
Vanderlan Nogueira Holanda ◽  
Antonio Yony Felipe Rodrigues ◽  
Caíque Silveira Martins da Fonseca ◽  
...  
Keyword(s):  
Essential Oil ◽  
Intestinal Motility ◽  
Scientific Evidence ◽  
Syzygium Aromaticum ◽  
Chemical Identification ◽  
Coagulation Time ◽  
Analysis And Evaluation ◽  
Ms Analysis ◽  
Biologic Effects ◽  
Clove Essential Oil

Clove (Syzygium aromaticum) is a spice widely used for its medical properties, though the species lacks scientific evidence regarding its toxicity and biologic effects. The aim of this study was the chemical identification by GC-MS analysis and evaluation of the hemolytic, anticoagulant, antidiarrheal and antipyretic activities of the essential oil from S. aromaticum (EOSa) in adult male mice. Essential oil was obtained by hydrodistillation and provided 9.8% v/w yield. GC-MS analyses allowed the identification of nine constituents, with eugenol (84.63%) as the majority. EOSa was diluted in several concentrations (0.005–2 mg/mL) for hemolytic assays, showing hemolytic activity above 20% in concentrations higher than 0.625 mg/mL. Different concentrations of EOSa induced a coagulation time 100% higher than control blood. 50 and 100 mg/kg of EOSa caused additional intestinal motility induced by castor oil by 90–100%. Fever, induced by Saccharomyces cerevisae 15% (s.c.), was controlled by 50 and 100 mg/kg EOSa (p.o.), effects similar to 100 mg/kg dypirone. Results showed that when used orally, EOSa may have a certain degree of toxicity in high dosages, but with antipyretic and intestinal motility properties.

Antioxidant activity of caffeoyl quinic acid derivatives from the roots of Dipsacus asper Wall

2006 ◽  
Vol 108 (2) ◽  
pp. 188-192 ◽  
Author(s):  
Tran Manh Hung ◽  
MinKyun Na ◽  
Phuong Thien Thuong ◽  
Nguyen Duy Su ◽  
DaiEun Sok ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quinic Acid ◽  
Dipsacus Asper ◽  
Caffeoyl Quinic Acid

Deodorization with Ku-ding-cha Containing a Large Amount of Caffeoyl Quinic Acid Derivatives

2004 ◽  
Vol 52 (17) ◽  
pp. 5513-5518 ◽  
Author(s):  
Osamu Negishi ◽  
Yukiko Negishi ◽  
Fumiyoshi Yamaguchi ◽  
Tatsuyuki Sugahara
Keyword(s):  
Quinic Acid ◽  
Caffeoyl Quinic Acid
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