scholarly journals Raw and Fermented Alfalfa Brown Juice Induces Changes in the Germination and Development of French Marigold (Tagetes patula L.) Plants

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1076
Author(s):  
Döme Barna ◽  
Szilvia Kisvarga ◽  
Szilvia Kovács ◽  
Gábor Csatári ◽  
Ibolya O. Tóth ◽  
...  
Keyword(s):  
Seed Germination ◽  
Tap Water ◽  
Germination Rate ◽  
Ornamental Plants ◽  
Dry Mass ◽  
Water Soluble ◽  
Tagetes Patula ◽  
Root And Shoot Length ◽  
Horticultural Practice ◽  
Brown Juice

Organic and ecological farming programs require new and efficient biostimulants with beneficial properties for the sustainable and safe production of seedlings and ornamental plants. We examined the effect of non-fermented and lacto-fermented alfalfa brown juice (BJ) on seed germination and the vegetative, physiological, and anatomical properties of French marigold (Tagetes patula L. ‘Csemő’) plants which were treated with 0.5–10% fermented and non-fermented BJ, with tap water applied as a control. Applying 0.5% fermented BJ significantly improved seed germination compared with non-fermented BJ, resulting in an increase of 9.6, 11.2, 10.9, and 41.7% in the final germination percent, germination rate index, germination index, and vigor index, respectively. In addition, it increased the root and shoot length by 7.9 and 16.1%, respectively, root and shoot dry mass by 20 and 47.6%, respectively, and the number of leaves by 28.8% compared to the control. Furthermore, an increase in contents of water-soluble phenol, chlorophyll a and b, and carotenoid was reported upon the application of 0.5% fermented BJ, while peroxidase activity decreased. Our results prove that alfalfa BJ can be enrolled as a biostimulant as part of the circular farming approach which supports the sustainable horticultural practice.

scholarly journals Effect of medicinal plant extracts on seed germination and seed associated mycoflora of wheat (Triticum aestivum L.)

2019 ◽  
Vol 27 ◽  
pp. 23-30
Author(s):  
MF Mahal ◽  
MS Akter
Keyword(s):  
Seed Germination ◽  
Plant Extracts ◽  
Germination Rate ◽  
Trichoderma Viride ◽  
Bipolaris Sorokiniana ◽  
Tagetes Patula ◽  
Wheat Varieties ◽  
Vigor Index ◽  
Medicinal Plant Extracts

The experiment was carried to evaluate the efficacy of five different plant extracts i.e. Adhatida vasica, Tagetes patula, Vinca rosea, Lowsonia alba and Andrographis paniculuta on seed germination, vigor index and seed borne and seed associated mycoflora of wheat varieties viz. Sourav and Prodip in in vitro condition. Seed health test of the collected wheat seed revealed that the seed was associated with six different fungi viz. Bipolaris sorokiniana, Fusarium sp., Aspergillus spp., Trichoderma viride, Penicillium sp. and Rhizopus sp. Wheat seeds were treated by dipping separately into different plant extracts of 1:1, 1:2 and 1:3 dilutions. Among the plant extracts, Lowsonia alba leaf extract was observed most effective followed by A. vasica, T. patula, V. rosea and A. paniculuta in reducing seed borne and seed associated mycoflora and also increased the germination rate and vigor index. J. bio-sci. 27: 23-30, 2019

scholarly journals Bioregulators on seed germination and seedling growth of sweet basil

2020 ◽  
Vol 11 ◽  
pp. e3324
Author(s):  
Larissa Santos Castro ◽  
Danúbia Aparecida Costa Nobre ◽  
Daniel Andrés Villegas Hurtado ◽  
Willian Rodrigues Macedo
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Germination Rate ◽  
Ocimum Basilicum ◽  
Shoot Length ◽  
Naphthalene Acetic Acid ◽  
Initial Growth ◽  
Randomized Design ◽  
Root And Shoot Length ◽  
Completely Randomized Design

Basil (Ocimum basilicum L.) plants have multiple uses, ranging from spice to cosmetic purpose, besides being a source of essential oil and aromatic. These plants were commonly propagated by seed, however, few agrotechnologies are used to enhance the seed germination and initial growth in this crop. For this reason, our research aimed to evaluate aspects of seed germination of Ocimum basilicum L. cv. Limoncino, subjected to different bioregulators applied via substrate imbibition. The experiment was carried out in completely randomized design, with four treatments, as follows: T1: control (distilled water), T2: T13 a solution of a product formulated based on indolbutyric acid + naphthalene acetic acid + gibberellic acid (0.2 mL L-1, Liko Química), T3: gibberellic acid (0.5 mM L-1, progibb-400®) and T4: brassinosteroids (0.1 mM L-1). We evaluated: germination rate, germination speed index (GSI), first count, root and shoot length and chlorophyll a, b, total and carotenoids. The bioregulators did not influence germination, first count, GSI and photosynthetic leaf pigment, but application of gibberellic acid and brassinosteroids improve the shoot and root length, respectively. The gibberellic acid and brassinosteroids are promising bioregulators for stimulating initial basil growth.

scholarly journals Composted Green Waste as a Container Medium Amendment for the Production of Ornamental Plants

HortScience ◽  
1997 ◽  
Vol 32 (1) ◽  
pp. 57-60 ◽  
Author(s):  
D.W. Burger ◽  
T.K. Hartz ◽  
G.W. Forister
Keyword(s):  
Seed Germination ◽  
Growth And Development ◽  
Petunia Hybrida ◽  
Ornamental Plants ◽  
Dry Mass ◽  
Growth Characteristics ◽  
Green Waste ◽  
Juniperus Sabina ◽  
Germinating Seeds ◽  
Pittosporum Tobira

Seed germination and crop growth characteristics were determined for Tagetes spp. L. `Lemondrop', marigold; Catharanthus roseus Don. `Little Pinkie', vinca; Petunia hybrida Vilm. `Royalty Cherry', petunia; Dendranthema×grandiflorum (Ramat.) Kitamura `White Diamond', chrysanthemum; Pittosporum tobira Ait. `Wheeleri', sweet mock orange; Photinia ×fraseri Dress., photinia and Juniperus sabina L. `Moon Glow', juniper grown in various size containers containing blends of composted green waste (CGW) and UC Mix. Seed germination, plant height, and stem and root fresh and dry mass were lowest in unamended CGW. For most plants studied, a CGW: UC Mix blend containing at least 25% UC Mix was required for adequate growth and development. Germinating seeds and young seedlings were most adversely affected by unamended CGW. As plants grew and were transplanted into larger containers (10- and 15-cm pots, 530 and 1800 mL), they were better able to grow in media with higher CGW content.

scholarly journals In-vitro Seed Germination Biology of Milicia Excelsa Population Collected from Benchi-Maji Zone, South Western Ethiopia

2020 ◽  
Author(s):  
MOHAMMED ADEFA SEID ◽  
Yigardu Mulatu ◽  
Agena Anjulo ◽  
Semaigzer Ayalew ◽  
Marshet Nigatu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Tap Water ◽  
Germination Rate ◽  
Germination Percentage ◽  
Hot Water ◽  
Laboratory Conditions ◽  
Southwestern Ethiopia ◽  
Micro Propagation ◽  
Milicia Excelsa

Abstract Background: In Ethiopia, species used for timber production are limited to few species. So, it is becoming necessary to find innovative ways of maintaining and improving the genetic quality of species such as Milicia excelsa by domesticating them into a wider scale of ecological and social environment. Hence, the main objective of this study was to investigate the seed germination biology and behavior of Milicia excelsa under in-vitro laboratory conditions so as to promote the domestication and establishment of seed production areas (SPA) in-situ. Methods: Seeds collected from Benchi district of Benchi-Maji zone of southwestern Ethiopia were subjected to germination biology in-vitro to determine the germination responses of M. excelsa seeds under laboratory conditions. So, a total of 1200 seeds were randomly selected, treated and prepared using four treatments procedures with six replications for paper method and 3 replications for sand method, and tested using paper methods in CRD design. So, 600 seeds were tested using paper and sand methods each where 25 seeds were tested for each replication in paper and 50 seeds for each replication in sand method. The germination process was lasted between 19 March 2020 and 15 May 2020. Result: The mean germination percentage of seeds of M. excelsa was noticed to be lower than 20%. This is below the expected (45%) germination rate of mature and healthy seed lot. Overall, seeds that were not treated using any pre-sowing treatments has shown relatively better germination percentage (19%) followed by seeds rubbed by hand (15%). Analysis of one-way t-test shows that the effects untreated (control –T1) and rubbing by hand treatments (T4) is significant at p < 0.05 compared to the effects of the other pre-sowing treatments (T2- seeds only washed with normal tap water, and T3- seeds washed and soaked in hot water for 15 minutes) on the germination capability of seeds of M. excelsa . However, no significance difference was observed between control (T1) and hand scarification (T2). Conclusions: The average germination of M. excelsa seeds combined for all treatments is lower than 20% which is lower germination when comparing with other similar research reported in other countries. Therefore, it required to include and test other pre-sowing treatments including tissue culture micro-propagation of seeds to maximize the germination of M. excelsa seeds helping to get enough seedlings required for the domestication project.

scholarly journals Magnetic Field Induced Changes in the Shoot and Root Proteome of Barley (Hordeum vulgare L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Azita Shabrangy ◽  
Arindam Ghatak ◽  
Shuang Zhang ◽  
Alfred Priller ◽  
Palak Chaturvedi ◽  
...  
Keyword(s):  
Seed Germination ◽  
Metal Ion ◽  
Positive Impact ◽  
Germination Rate ◽  
Redox Homeostasis ◽  
Reduction Process ◽  
Plant Evolution ◽  
Metal Ion Binding ◽  
Hordeum Vulgare L ◽  
Root And Shoot Length

The geomagnetic field (GMF) has been present since the beginning of plant evolution. Recently, some researchers have focused their efforts on employing magnetic fields (MFs) higher than GMF to improve the seed germination, growth, and harvest of agriculturally important crop plants, as the use of MFs is an inexpensive and environment-friendly technique. In this study, we have employed different treatments of MF at 7 mT (milliTesla) at different time points of exposure, including 1, 3, and 6 h. The extended exposure was followed by five consecutive days at 6 h per day in barley seeds. The results showed a positive impact of MF on growth characteristics for 5-day-old seedlings, including seed germination rate, root and shoot length, and biomass weight. Furthermore, ~5 days of delay of flowering in pre-treated plants was also observed. We used a shotgun proteomics approach to identify changes in the protein signatures of root and shoot tissues under MF effects. In total, we have identified 2,896 proteins. Thirty-eight proteins in the shoot and 15 proteins in the root showed significant changes under the MF effect. Proteins involved in primary metabolic pathways were increased in contrast to proteins with a metal ion binding function, proteins that contain iron ions in their structure, and proteins involved in electron transfer chain, which were all decreased significantly in the treated tissues. The upregulated proteins' overall biological processes included carbohydrate metabolic process, oxidation-reduction process, and cell redox homeostasis, while down-regulated processes included translation and protein refolding. In general, shoot response was more affected by MF effect than root tissue, leading to the identification of 41 shoot specific proteins. This study provides an initial insight into the proteome regulation response to MF during barley's seedling stage.

scholarly journals Comparison of Seed Germination, Root and Shoot Length of Maize and Wheat Crops with Compost Leachate and Commercial Fertilizer

2019 ◽  
Vol 01 (03) ◽  
pp. 15-22
Author(s):  
Ayesha Ameen ◽  
Omer Mushtaq
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Shoot Length ◽  
Commercial Fertilizer ◽  
Maize Crop ◽  
Environment Friendly ◽  
Important Concern ◽  
Root And Shoot Length ◽  
Compost Leachate ◽  
Experimental Trials

The increasing public concern about municipal solid waste (MSW) due to its impact on health and environment induces people to think about more ways of recycling this waste. These days, people strive to find more appropriate methods and processes to treat MSW for its proper management and disposal. Composting process is the most appropriate method of recycling used to get rid of organic fractions. The most important concern is the production of compost leachate. This study was designed to check the potential of compost leachate as a chemical fertilizer and to compare it with commercial fertilizer. Different dilutions of compost leachate and commercial fertilizer were used to check their effects on seed germination of wheat and maize crop and the initial root and shoot length measurement of these crops. Germination index, germination inhibition and germination rate were also evaluated in different experimental trials with variation in leachate dilution. The results showed best root, shoot length and seed germination at low quantity of leachate sample. The obtained results showed that leachate has better fertilizer qualities as compared to commercial fertilizer. Moreover, it is more economical and environment friendly. It also helps in maintaining the structure of soil and conditions the soil.

scholarly journals Effect of Pre-Sowing Treatments on Seed Germination and Seedling Growth of Silver Butterfly Tree (Bauhinia rufescens)

2018 ◽  
Vol 6 (3) ◽  
pp. 344-354
Author(s):  
J. A. Opoku ◽  
J. N. Amissah ◽  
M. E. Essilfie ◽  
J. C. Norman
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Regulator ◽  
Tap Water ◽  
Germination Rate ◽  
Germination Percentage ◽  
Hot Water ◽  
Root Weight ◽  
Number Of Leaves ◽  
Shoot Weight

Three experiments were conducted in a plastic greenhouse at the Sinna Garden of the Crop Science Department of the University of Ghana, Legon, Accra to investigate into pre-sowing treatment (water treatment, growth regulator, and sulphuric acid scarification and water soaking) effects on seed germination and seedling growth of Bauhinia rufescens from October to December, 2011 and January to April, 2012, respectively. The experimental design used was complete randomized design. There were 4 treatments in experiment 1, 7 in experiment 2 and 6 in experiment 3. Each experiment was replicated five (5) times. The results showed that Bauhinia rufescens seeds soaked in hot water at 65 0 C for 60 minutes gave the highest mean germination rate, tallest plant height and highest number of leaves per plant at 49 days after soaking. It also had the widest stem diameter, highest fresh shoot and root weight, highest fresh root and dry root weight and number of roots per seedling. Seeds soaked in 500 ppm Gibberelic acid(GA3) produced the highest number of leaves per plant and tallest plants at 49 DAS. Seeds soaked in 750 ppm GA3 had the highest number of leaves per plant at 49 DAS and highest fresh shoot weight. Seeds soaked in 1000 ppm Promalin differed significantly from 500 ppm GA3 and the control in germination percentage. Seeds soaked in 1000 ppm Promalin differed significantly from 500 ppm GA3 and the control in germination percentage. Acid scarified seeds for 60 minutes plus soaking in tap water for 24 hours differed significantly from the seeds treated withother growth regulator rates plus 24 hours soaking in germination percentage. Acid scarified seeds for 45 minutes plus soaking in tap water for 24 hours had the tallest plant and highest number of leaves per plant at 49 DAS, widest stem diameter, highest fresh shoot and root weight and dry shoot weight. Soaking seeds in hot water at 65 0 C for 60 minutes and/or in acid (H2SO4) for 45 minutes plus soaking in tap water for 24 hours is recommended as pre-sowing treatment for Bauhinia rufescens for maximum mean germination rate and vigorous vegetative growth of seedlings.

scholarly journals Drought Stresses on Seed Germination and Early Growth of Maize and Sorghum

2019 ◽  
Vol 11 (2) ◽  
pp. 310 ◽  
Author(s):  
Mariana S. Queiroz ◽  
Carlos E. S. Oliveira ◽  
Fábio Steiner ◽  
Alan M. Zuffo ◽  
Tiago Zoz ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Osmotic Potential ◽  
Germination Rate ◽  
Early Growth ◽  
Root Shoot Ratio ◽  
Randomized Design ◽  
Root And Shoot Length ◽  
Early Seedling ◽  
Effects Of Drought

Seeds of maize (Zea mays L.) and sorghum [Sorghum bicolor (L.) Moench.] were submitted to different osmotic potential levels induced by polyethylene glycol (PEG) with the objective of evaluating the effects of drought stress on seed germination and early seedling growth. Seeds were arranged in paper rolls and soaked in PEG solutions prepared with osmotic potentials 0.0 (control), -0.2, -0.4, and -0.8 MPa and kept into a seed germinator, at 25 &deg;C for 18 days. A completely randomized design in a 2 &times; 4 factorial scheme with four replications of 50 seeds each was used. The results showed that by increasing of the osmotic potential level, germinated seed number, germination rate index, root and shoot length, shoot and root dry matter, and seedling vigor index (SVI) decreased, while mean germination time (MGT) and root: shoot ratio (RSR) increased in both crops. Additionally, the maize was more susceptible than sorghum to drought stress, with germination response declining more rapidly with decreasing osmotic potential. Sorghum crop tolerates water stress of up to -0.2 MPa, without reducing germination of the seeds; however, the growth of shoots and roots are inhibited. Drought stress limits the process of seed germination and early growth of maize seedlings.

scholarly journals Calcium Nitrate Priming Increases the Germination Rate of Eggplant Seeds

2019 ◽  
Vol 11 (15) ◽  
pp. 181
Author(s):  
Josiane S. Salles ◽  
Alexandre H. F. de Lima ◽  
Flávio F. da S. Binotti ◽  
Edilson Costa ◽  
Eliana D. C. Binotti ◽  
...  
Keyword(s):  
Seed Germination ◽  
Emission Rate ◽  
Germination Rate ◽  
Calcium Nitrate ◽  
Dry Mass ◽  
Chemical Agents ◽  
Randomized Design ◽  
Germination Process ◽  
Adverse Environmental Conditions ◽  
Control Water

The priming may promote greater expression of the physiological potential of the seeds and contribute to the germination process under adverse environmental conditions. The objective of this study was to evaluate the physiological potential of eggplant seeds primed with different chemical agents and exposed to adverse temperatures. Seeds were subjected to priming with five chemical agents [unprimed (control); water (hydropriming); calcium nitrate (0.2%); amino acid L-phenylalanine (0.05%); L-phenylalanine (0.05%) + calcium nitrate (0.2%)] and at temperatures of 15, 25, 35 and 41 &ordm;C, considering a completely randomized design in a factorial arrangement (5 &times; 4) with four replicates. The first count of radicle emission and germination, final radicle emission, seed germination, radicle emission rate index, mean radicle emission time, and seedling dry mass were measured. Germination process of eggplant seeds was completely inhibited at 41 &ordm;C, and the optimal temperature for seed germination is 25 &ordm;C. Calcium nitrate priming potentiates the seed germination process, resulting in higher radicle emission rate index and higher germination rate. Low temperature (15 &ordm;C) has greater interference in the germination rate of eggplant seeds when compared to high temperature (35 &ordm;C).

scholarly journals Allelopathic Effects of Sphaeranthus suaveolens on Seed Germination and Seedling Growth of Phaseolus vulgaris and Oryza sativa

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hudson C. Laizer ◽  
Musa N. Chacha ◽  
Patrick A. Ndakidemi
Keyword(s):  
Oryza Sativa ◽  
Phaseolus Vulgaris ◽  
Seed Germination ◽  
Chlorophyll Content ◽  
Seedling Growth ◽  
Crude Extract ◽  
Water Soluble ◽  
Crude Extracts ◽  
Root And Shoot Length ◽  
Allelopathic Effects

Weeds with allelopathic effect have been reported to cause significant damage in agriculture particularly in smallholder farming systems. This study assessed the allelopathic effects of different concentrations of crude extract of a noxious weed Sphaeranthus suaveolens on seed germination and seedling growth of Phaseolus vulgaris and Oryza sativa by examining germination, seedling height, and total chlorophyll content after seven and fourteen days of crude extract treatment, respectively. Results showed that seed germination and seedling growth were significantly ( p < 0.001 ) decreased with increase in concentration of crude extract, signifying concentration dependency. Highest concentration (100%) of S. suaveolens crude extracts resulted in 90% and 100% inhibition of P. vulgaris and O. sativa seed germination, respectively. Chlorophyll content, fresh weight, and root and shoot length of both P. vulgaris and O. sativa were also significantly ( p < 0.001 ) affected by highest concentration (100%) of S. suaveolens crude extracts. Results from this study suggest that the extract of S. suaveolens contains water-soluble allelochemicals which significantly reduce growth and productivity of P. vulgaris and O. sativa.

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