scholarly journals Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa)

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1665
Author(s):  
Marwa Louati ◽  
Blanca Salazar-Sarasua ◽  
Edelín Roque ◽  
José Pío Beltrán ◽  
Amel Salhi Hannachi ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Functional Characterization ◽  
Reproductive Development ◽  
Genetic Network ◽  
Floral Organ ◽  
Local Economy ◽  
Mads Box ◽  
Argania Spinosa ◽  
Mads Box Gene

Argan trees (Argania spinosa) belong to a species native to southwestern Morocco, playing an important role in the environment and local economy. Argan oil extracted from kernels has a unique composition and properties. Argan trees were introduced in Tunisia, where hundreds of trees can be found nowadays. In this study, we examined reproductive development in Argan trees from four sites in Tunisia and carried out the functional characterization of a floral homeotic gene in this non-model species. Despite the importance of reproductive development, nothing is known about the genetic network controlling flower development in Argania spinosa. Results obtained in several plant species established that floral organ development is mostly controlled by MADS-box genes and, in particular, APETALA3 (AP3) and PISTILLATA (PI) homologs are required for proper petal and stamen identity. Here, we describe the isolation and functional characterization of a MADS-box gene from Argania spinosa. Phylogenetic analyses showed strong homology with PI-like proteins, and the expression of the gene was found to be restricted to the second and third whorls. Functional homology with Arabidopsis PI was demonstrated by the ability of AsPI to confer petal and stamen identity when overexpressed in a pi-1 mutant background. The identification and characterization of this gene support the strong conservation of PI homologs among distant angiosperm plants.

scholarly journals Role of MADS-box Gene in the Development of Flower Organs in Pineapple of a Typical Collective Fruit

2020 ◽  
Author(s):  
hongna zhang ◽  
Xiaolu Pan ◽  
Debao Yi ◽  
Wenqiu Lin ◽  
Xiumei Zhang
Keyword(s):  
Functional Characterization ◽  
Floral Organ ◽  
Type I ◽  
Type Ii ◽  
Mads Box ◽  
Mads Box Genes ◽  
Floral Organs ◽  
Genome Survey ◽  
Mads Box Gene ◽  
Collective Fruit

Abstract Background: MADS-box genes play crucial roles in plant vegetative and reproductive growth, especially in inflorescences, flower, and fruit. Pineapple is a typical collective fruit, and a comprehensive analysis of the MADS-box gene family in the development of floral organs of pineapple is still lacking. Results: In this study, the whole-genome survey and expression profiling of the MADS-box family in pineapple were introduced. Forty-four AcMADS genes were identified in pineapple, 39 of them were located on 18 chromosomes and five genes were distributed in five scaffolds. Twenty-two AcMADS genes were defined as 15 pairs of segmental duplication events. Syntenic analysis showed that pineapple is closely related to monocotyledon plants. Most members of the type II subfamily of AcMADS genes had higher expression levels in floral organs compared with type I subfamily, thereby suggesting that AcMADS of type II may play more crucial roles in the development of floral organs of pineapple. Six AcMADS genes have significant tissue-specificity expression, thereby suggesting that they may participate in the formation of one or more floral organs. Conclusions: Our findings not only benefit to reveal the functional characterization of MADS-box genes in the floral organ development of pineapple but also provide additional information for further understanding the formation and development collective fruit.

scholarly journals A Cucumber AGAMOUS-LIKE 15 (AGL15) MADS-Box Gene Mediates Abnormal Leaf Morphology in Arabidopsis

Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 265
Author(s):  
Yong Zhou ◽  
Lingli Ge ◽  
Lifang Hu ◽  
Yingui Yang ◽  
Shiqiang Liu
Keyword(s):  
Functional Characterization ◽  
Ectopic Expression ◽  
Leaf Size ◽  
Reproductive Organs ◽  
Mads Box ◽  
Cucumis Sativus L ◽  
Reading Frame ◽  
Relationship Analysis ◽  
Mads Box Gene

The AGL15 subfamily MADS-box proteins play vital roles in various developmental processes, such as floral transition, somatic embryogenesis, and leaf and fruit development. In this work, an AtAGL15 ortholog, CsMADS26, was cloned from cucumber (Cucumis sativus L.). The open reading frame (ORF) of CsMADS26 is 669 bp in length, encoding a predicted protein of 222 amino acids. The CsMADS26 protein contains a highly conserved MADS-box domain and a variable C domain, as well as less conserved I and K domains. Phylogenetic relationship analysis revealed that CsMADS26 was clustered into the AGL15 clade of AGL15 subfamily. Expression analysis based on qRT-PCR showed that CsMADS26 is mainly expressed in reproductive organs including flowers and fruits. Transgenic Arabidopsis plants with ectopic expression of CsMADS26 exhibited curled rosette and cauline leaves, and the leaf size was much smaller than that of wild-type (WT) plants. These results provide clues for the functional characterization of CsMADS26 in the future.

scholarly journals Genome-Wide Analysis of the MADS-Box Transcription Factor Family in Solanum lycopersicum

2019 ◽  
Vol 20 (12) ◽  
pp. 2961 ◽  
Author(s):  
Yunshu Wang ◽  
Jianling Zhang ◽  
Zongli Hu ◽  
Xuhu Guo ◽  
Shibing Tian ◽  
...  
Keyword(s):  
Gene Family ◽  
Fruit Development ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Constitutive Expression ◽  
Floral Organ ◽  
Mads Box ◽  
Mads Box Genes ◽  
Fruit Development And Ripening ◽  
Mads Box Gene

MADS-box family genes encode transcription factors that are involved in multiple developmental processes in plants, especially in floral organ specification, fruit development, and ripening. However, a comprehensive analysis of tomato MADS-box family genes, which is an important model plant to study flower fruit development and ripening, remains obscure. To gain insight into the MADS-box genes in tomato, 131 tomato MADS-box genes were identified. These genes could be divided into five groups (Mα, Mβ, Mγ, Mδ, and MIKC) and were found to be located on all 12 chromosomes. We further analyzed the phylogenetic relationships among Arabidopsis and tomato, as well as the protein motif structure and exon–intron organization, to better understand the tomato MADS-box gene family. Additionally, owing to the role of MADS-box genes in floral organ identification and fruit development, the constitutive expression patterns of MADS-box genes at different stages in tomato development were identified. We analyzed 15 tomato MADS-box genes involved in floral organ identification and five tomato MADS-box genes related to fruit development by qRT-PCR. Collectively, our study provides a comprehensive and systematic analysis of the tomato MADS-box genes and would be valuable for the further functional characterization of some important members of the MADS-box gene family.

Isolation and molecular characterization of a new vegetative MADS-box gene from Solanum tuberosum L.

Planta ◽  
1998 ◽  
Vol 207 (2) ◽  
pp. 181-188 ◽  
Author(s):  
María José Carmona ◽  
Nely Ortega ◽  
Federico Garcia-Maroto
Keyword(s):  
Solanum Tuberosum ◽  
Molecular Characterization ◽  
Solanum Tuberosum L ◽  
Mads Box ◽  
Mads Box Gene

scholarly journals Isolation and Characterization of a MADS-box Gene cDNA, PnMADS1, That is Expressed in Both Vegetative and Floral Meristems of Pharbitis nil.

2000 ◽  
Vol 17 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Michiyuki ONO ◽  
Kimiyo SAGE-ONO ◽  
Wakako MARUYAMA ◽  
Michihiro KAWAKAMI ◽  
Masayasu INOUE ◽  
...  
Keyword(s):  
Pharbitis Nil ◽  
Mads Box ◽  
Isolation And Characterization ◽  
Mads Box Gene ◽  
Floral Meristems

Characterization of PTM5 in aspen trees: a MADS-box gene expressed during woody vascular development

Gene ◽  
2003 ◽  
Vol 318 ◽  
pp. 55-67 ◽  
Author(s):  
Leland J. Cseke ◽  
Jun Zheng ◽  
Gopi K. Podila
Keyword(s):  
Vascular Development ◽  
Mads Box ◽  
Mads Box Gene

scholarly journals Pitfall Flower Development and Organ Identity of Ceropegia sandersonii (Apocynaceae-Asclepiadoideae)

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1767
Author(s):  
Annemarie Heiduk ◽  
Dewi Pramanik ◽  
Marlies Spaans ◽  
Loes Gast ◽  
Nemi Dorst ◽  
...  
Keyword(s):  
Gene Expression ◽  
Floral Anatomy ◽  
Floral Organ ◽  
Mads Box ◽  
Floral Organs ◽  
Abcde Model ◽  
Organ Identity ◽  
Mads Box Gene ◽  
Flower Evolution ◽  
Developmental Phases

Deceptive Ceropegia pitfall flowers are an outstanding example of synorganized morphological complexity. Floral organs functionally synergise to trap fly-pollinators inside the fused corolla. Successful pollination requires precise positioning of flies headfirst into cavities at the gynostegium. These cavities are formed by the corona, a specialized organ of corolline and/or staminal origin. The interplay of floral organs to achieve pollination is well studied but their evolutionary origin is still unclear. We aimed to obtain more insight in the homology of the corona and therefore investigated floral anatomy, ontogeny, vascularization, and differential MADS-box gene expression in Ceropegia sandersonii using X-ray microtomography, Light and Scanning Electronic Microscopy, and RT-PCR. During 10 defined developmental phases, the corona appears in phase 7 at the base of the stamens and was not found to be vascularized. A floral reference transcriptome was generated and 14 MADS-box gene homologs, representing all major MADS-box gene classes, were identified. B- and C-class gene expression was found in mature coronas. Our results indicate staminal origin of the corona, and we propose a first ABCDE-model for floral organ identity in Ceropegia to lay the foundation for a better understanding of the molecular background of pitfall flower evolution in Apocynaceae.

scholarly journals Functional Characterization of BRASSINAZOLE-RESISTANT 1 in Panax Ginseng (PgBZR1) and Brassinosteroid Response during Storage Root Formation

2020 ◽  
Vol 21 (24) ◽  
pp. 9666
Author(s):  
Hyeona Hwang ◽  
Hwa-Yong Lee ◽  
Hojin Ryu ◽  
Hyunwoo Cho
Keyword(s):  
Panax Ginseng ◽  
Glycogen Synthase ◽  
Functional Characterization ◽  
Secondary Growth ◽  
Ectopic Expression ◽  
Floral Organ ◽  
Membrane Receptors ◽  
Storage Root ◽  
Functional Conservation

Brassinosteroids (BRs) play crucial roles in the physiology and development of plants. In the model plant Arabidopsis, BR signaling is initiated at the level of membrane receptors, BRASSINOSTEROIDS INSENSITIVE 1 (BRI1) and BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) complex, thus activating the transcription factors (TFs) BRASSINAZOLE RESISTANT 1/BRI1-EMS-SUPPRESSOR 1 (BZR1/BES1) to coordinate BR responsive genes. BRASSINOSTEROIDS INSENSITIVE 2 (BIN2), glycogen synthase kinase 3 (GSK3) like-kinase, negatively regulates BZR1/BES1 transcriptional activity through phosphorylation-dependent cytosolic retention and shuttling. However, it is still unknown whether this mechanism is conserved in Panax ginseng C. A. Mayer, a member of the Araliaceae family, which is a shade-tolerant perennial root crop. Despite its pharmacological and agricultural importance, the role of BR signaling in the development of P. ginseng and characterization of BR signaling components are still elusive. In this study, by utilizing the Arabidopsisbri1 mutant, we found that ectopic expression of the gain of function form of PgBZR1 (Pgbzr1-1D) restores BR deficiency. In detail, ectopic expression of Pgbzr1-1D rescues dwarfism, defects of floral organ development, and hypocotyl elongation of bri1-5, implying the functional conservation of PgBZR1 in P. ginseng. Interestingly, brassinolide (BL) and BRs biosynthesis inhibitor treatment in two-year-old P. ginseng storage root interferes with and promotes, respectively, secondary growth in terms of xylem formation. Altogether, our results provide new insight into the functional conservation and potential diversification of BR signaling and response in P. ginseng.

scholarly journals A Lysine Substitute for K+

2002 ◽  
Vol 277 (51) ◽  
pp. 49651-49654 ◽  
Author(s):  
Georgiy A. Belogurov ◽  
Reijo Lahti
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Phylogenetic Analyses ◽  
Functional Characterization ◽  
Membrane Vesicles ◽  
Thermophilic Bacterium ◽  
Inorganic Pyrophosphatase ◽  
Inner Membrane ◽  
Carboxydothermus Hydrogenoformans

The H+proton-translocating inorganic pyrophosphatase (H+-PPase) family is composed of two phylogenetically distinct types of enzymes: K+-dependent and K+-independent. However, to date, the sequence criteria governing this dichotomy have remained unknown. In this study, we describe the heterologous expression and functional characterization of H+-PPase from the thermophilic bacteriumCarboxydothermus hydrogenoformans. Both PPi-hydrolyzing and PPi-energized H+translocation activities of the recombinant enzyme inEscherichia coliinner membrane vesicles are strictly K+-dependent. Here we deduce the K+requirement of all available H+-PPase sequences based on the K+dependence ofC. hydrogenoformansH+-PPase in conjunction with phylogenetic analyses. Our data reveal that K+-independent H+-PPases possess conserved Lys and Thr that are absent in K+-dependent H+-PPases. We further demonstrate that a A460K substitution inC. hydrogenoformansH+-PPase is sufficient to confer K+independence to both PPihydrolysis and PPi-energized H+translocation. In contrast, a A463T mutation does not affect the K+dependence of H+-PPase.

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