scholarly journals Genome-Wide Analysis of the MADS-Box Transcription Factor Family in Solanum lycopersicum

2019 ◽  
Vol 20 (12) ◽  
pp. 2961 ◽  
Author(s):  
Yunshu Wang ◽  
Jianling Zhang ◽  
Zongli Hu ◽  
Xuhu Guo ◽  
Shibing Tian ◽  
...  
Keyword(s):  
Gene Family ◽  
Fruit Development ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Constitutive Expression ◽  
Floral Organ ◽  
Mads Box ◽  
Mads Box Genes ◽  
Fruit Development And Ripening ◽  
Mads Box Gene

MADS-box family genes encode transcription factors that are involved in multiple developmental processes in plants, especially in floral organ specification, fruit development, and ripening. However, a comprehensive analysis of tomato MADS-box family genes, which is an important model plant to study flower fruit development and ripening, remains obscure. To gain insight into the MADS-box genes in tomato, 131 tomato MADS-box genes were identified. These genes could be divided into five groups (Mα, Mβ, Mγ, Mδ, and MIKC) and were found to be located on all 12 chromosomes. We further analyzed the phylogenetic relationships among Arabidopsis and tomato, as well as the protein motif structure and exon–intron organization, to better understand the tomato MADS-box gene family. Additionally, owing to the role of MADS-box genes in floral organ identification and fruit development, the constitutive expression patterns of MADS-box genes at different stages in tomato development were identified. We analyzed 15 tomato MADS-box genes involved in floral organ identification and five tomato MADS-box genes related to fruit development by qRT-PCR. Collectively, our study provides a comprehensive and systematic analysis of the tomato MADS-box genes and would be valuable for the further functional characterization of some important members of the MADS-box gene family.

Genome-wide identification and analysis of the MADS-box gene family and its potential role in fruit ripening in black raspberry (Rubus occidentalis L.)

2021 ◽  
pp. 1-15
Author(s):  
Yaqiong Wu ◽  
Chunhong Zhang ◽  
Wenlong Wu ◽  
Weilin Li ◽  
Lianfei Lyu
Keyword(s):  
Gene Family ◽  
Fruit Ripening ◽  
Fruit Development ◽  
Expression Patterns ◽  
Type I ◽  
Black Raspberry ◽  
Mads Box ◽  
Mads Box Genes ◽  
Genome Wide ◽  
Mads Box Gene

BACKGROUND: Black raspberry is a vital fruit crop with a high antioxidant function. MADS-box genes play an important role in the regulation of fruit development in angiosperms. OBJECTIVE: To understand the regulatory role of the MADS-box family, a total of 80 MADS-box genes were identified and analyzed. METHODS: The MADS-box genes in the black raspberry genome were analyzed using bioinformatics methods. Through an analysis of the promoter elements, the possible functions of different members of the family were predicted. The spatiotemporal expression patterns of members of the MADS-box family during black raspberry fruit development and ripening were systematically analyzed. RESULTS: The genes were classified into type I (Mα: 33; Mβ: 6; Mγ: 10) and type II (MIKC *: 2; MIKCC: 29) genes. We also obtained a complete overview of the RoMADS-box gene family through phylogenetic, gene structure, conserved motif, and cis element analyses. The relative expression analysis showed different expression patterns, and most RoMADS-box genes were more highly expressed in fruit than in other tissues of black raspberry. CONCLUSIONS: This finding indicates that the MADS-box gene family is involved in the regulation of fruit ripening processes in black raspberry.

scholarly journals Role of MADS-box Gene in the Development of Flower Organs in Pineapple of a Typical Collective Fruit

2020 ◽  
Author(s):  
hongna zhang ◽  
Xiaolu Pan ◽  
Debao Yi ◽  
Wenqiu Lin ◽  
Xiumei Zhang
Keyword(s):  
Functional Characterization ◽  
Floral Organ ◽  
Type I ◽  
Type Ii ◽  
Mads Box ◽  
Mads Box Genes ◽  
Floral Organs ◽  
Genome Survey ◽  
Mads Box Gene ◽  
Collective Fruit

Abstract Background: MADS-box genes play crucial roles in plant vegetative and reproductive growth, especially in inflorescences, flower, and fruit. Pineapple is a typical collective fruit, and a comprehensive analysis of the MADS-box gene family in the development of floral organs of pineapple is still lacking. Results: In this study, the whole-genome survey and expression profiling of the MADS-box family in pineapple were introduced. Forty-four AcMADS genes were identified in pineapple, 39 of them were located on 18 chromosomes and five genes were distributed in five scaffolds. Twenty-two AcMADS genes were defined as 15 pairs of segmental duplication events. Syntenic analysis showed that pineapple is closely related to monocotyledon plants. Most members of the type II subfamily of AcMADS genes had higher expression levels in floral organs compared with type I subfamily, thereby suggesting that AcMADS of type II may play more crucial roles in the development of floral organs of pineapple. Six AcMADS genes have significant tissue-specificity expression, thereby suggesting that they may participate in the formation of one or more floral organs. Conclusions: Our findings not only benefit to reveal the functional characterization of MADS-box genes in the floral organ development of pineapple but also provide additional information for further understanding the formation and development collective fruit.

scholarly journals 092 RNA Localization of a Strawberry MADS-Box Gene (SAG1) Involved in Fruit Development

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 457C-457 ◽  
Author(s):  
Faye M. Rosin ◽  
David Hannapel
Keyword(s):  
Fruit Development ◽  
Sequence Similarity ◽  
Floral Organ ◽  
Reproductive Organ ◽  
Amino Acid Sequence Similarity ◽  
Nucleotide Sequence Analysis ◽  
Mads Box ◽  
Mads Box Genes ◽  
Mads Box Gene ◽  
And Control

MADS-box genes are an important family of highly conserved regulatory genes in plants, animals, and yeast. Genetic analyses have shown that plant MADS-box genes are homeotic and control both the spatial and temporal location of specific organs. While MADS-box genes have been extensively studied and characterized in floral organ development, their involvement in other developmental processes, such as fruit development, is not well understood. From a strawberry fruit cDNA library, we have identified a strawberry AGAMOUS-like MADS-box gene (SAG1) that is expressed in developing fruit, but not in leaves. This is the first MADS-box gene to be isolated from strawberry. The hypothesis guiding this research is that SAG1 plays an important role in the development of the fruit. Nucleotide sequence analysis showed that this cDNA had the highest sequence match to genes from the AGAMOUS family. Comparison of amino acid sequence similarity between SAG1 and members of this family ranged from 70 to 75% overall, and between 98% to100% within the MADS-box. Involvement in stamen and carpel identity is one function of this family of MADS-box genes. Northern hybridizations were performed in order to analyze the expression of this gene at the RNA level. RNA was extracted from various organs of Fragaria ×ananassa, c.v. Calypso. SAG1 RNA expression was specific to stamens, carpels and all stages of fruit and seed development. No expression was detected in roots, leaves, or sepals. Thus, we conclude that SAG1 RNA is involved in reproductive organ and fruit development.

Characterization of 10 MADS-box genes from Pyrus pyrifolia and their differential expression during fruit development and ripening

Gene ◽  
2013 ◽  
Vol 528 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Benjamin Ewa Ubi ◽  
Takanori Saito ◽  
Songling Bai ◽  
Chikako Nishitani ◽  
Yusuke Ban ◽  
...  
Keyword(s):  
Differential Expression ◽  
Fruit Development ◽  
Pyrus Pyrifolia ◽  
Mads Box ◽  
Mads Box Genes ◽  
Fruit Development And Ripening

scholarly journals Transcript Profiling of MIKCc MADS-Box Genes Reveals Conserved and Novel Roles in Barley Inflorescence Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Hendrik N. J. Kuijer ◽  
Neil J. Shirley ◽  
Shi F. Khor ◽  
Jin Shi ◽  
Julian Schwerdt ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Floral Organ ◽  
Transcript Analysis ◽  
Mads Box ◽  
Mads Box Genes ◽  
Inflorescence Development ◽  
Abcde Model ◽  
Wide Range ◽  
Dicotyledonous Plants ◽  
Range Of Functions

MADS-box genes have a wide range of functions in plant reproductive development and grain production. The ABCDE model of floral organ development shows that MADS-box genes are central players in these events in dicotyledonous plants but the applicability of this model remains largely unknown in many grass crops. Here, we show that transcript analysis of all MIKCc MADS-box genes through barley (Hordeum vulgare L.) inflorescence development reveals co-expression groups that can be linked to developmental events. Thirty-four MIKCc MADS-box genes were identified in the barley genome and single-nucleotide polymorphism (SNP) scanning of 22,626 barley varieties revealed that the natural variation in the coding regions of these genes is low and the sequences have been extremely conserved during barley domestication. More detailed transcript analysis showed that MADS-box genes are generally expressed at key inflorescence developmental phases and across various floral organs in barley, as predicted by the ABCDE model. However, expression patterns of some MADS genes, for example HvMADS58 (AGAMOUS subfamily) and HvMADS34 (SEPALLATA subfamily), clearly deviate from predicted patterns. This places them outside the scope of the classical ABCDE model of floral development and demonstrates that the central tenet of antagonism between A- and C-class gene expression in the ABC model of other plants does not occur in barley. Co-expression across three correlation sets showed that specifically grouped members of the barley MIKCc MADS-box genes are likely to be involved in developmental events driving inflorescence meristem initiation, floral meristem identity and floral organ determination. Based on these observations, we propose a potential floral ABCDE working model in barley, where the classic model is generally upheld, but that also provides new insights into the role of MIKCc MADS-box genes in the developing barley inflorescence.

scholarly journals Genome-wide identification and characterization of the MADS-box gene family in Salix suchowensis

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8019 ◽  
Author(s):  
Yanshu Qu ◽  
Changwei Bi ◽  
Bing He ◽  
Ning Ye ◽  
Tongming Yin ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Profiles ◽  
Evolutionary Analysis ◽  
Mads Box ◽  
Mads Box Genes ◽  
Floral Organogenesis ◽  
Functional Studies ◽  
Genome Wide ◽  
Development Processes ◽  
Mads Box Gene

MADS-box genes encode transcription factors that participate in various plant growth and development processes, particularly floral organogenesis. To date, MADS-box genes have been reported in many species, the completion of the sequence of the willow genome provides us with the opportunity to conduct a comprehensive analysis of the willow MADS-box gene family. Here, we identified 60 willow MADS-box genes using bioinformatics-based methods and classified them into 22 M-type (11 Mα, seven Mβ and four Mγ) and 38 MIKC-type (32 MIKCc and six MIKC*) genes based on a phylogenetic analysis. Fifty-six of the 60 SsMADS genes were randomly distributed on 19 putative willow chromosomes. By combining gene structure analysis with evolutionary analysis, we found that the MIKC-type genes were more conserved and played a more important role in willow growth. Further study showed that the MIKC* type was a transition between the M-type and MIKC-type. Additionally, the number of MADS-box genes in gymnosperms was notably lower than that in angiosperms. Finally, the expression profiles of these willow MADS-box genes were analysed in five different tissues (root, stem, leave, bud and bark) and validated by RT-qPCR experiments. This study is the first genome-wide analysis of the willow MADS-box gene family, and the results establish a basis for further functional studies of willow MADS-box genes and serve as a reference for related studies of other woody plants.

scholarly journals Genome-Wide Analysis and the Expression Pattern of the MADS-Box Gene Family in Bletilla striata

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2184
Author(s):  
Ze-Yuan Mi ◽  
Qian Zhao ◽  
Chan Lu ◽  
Qian Zhang ◽  
Lin Li ◽  
...  
Keyword(s):  
Gene Family ◽  
Biological Activities ◽  
Expression Patterns ◽  
Perennial Herb ◽  
Mads Box ◽  
Bletilla Striata ◽  
Qpcr Analysis ◽  
Genome Wide ◽  
Mads Box Gene ◽  
Highly Correlated

Bletilla striata (Thunb. ex A. Murray) Rchb. f., a species of the perennial herb Orchidaceae, has potent anti-inflammatory and antiviral biological activities. MADS-box transcription factors play critical roles in the various developmental processes of plants. Although this gene family has been extensively investigated in many species, it has not been analyzed for B. striata. In total, 45 MADS-box genes were identified from B. striata in this study, which were classified into five subfamilies (Mδ, MIKC, Mα, Mβ, and Mγ). Meanwhile, the highly correlated protein domains, motif compositions, and exon–intron structures of BsMADSs were investigated according to local B. striata databases. Chromosome distribution and synteny analyses revealed that segmental duplication and homologous exchange were the main BsMADSs expansion mechanisms. Further, RT-qPCR analysis revealed that BsMADSs had different expression patterns in response to various stress treatments. Our results provide a potential theoretical basis for further investigation of the functions of MADS genes during the growth of B. striata.

scholarly journals Isolation and Functional Analysis of a PISTILLATA-like MADS-Box Gene from Argan Tree (Argania spinosa)

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1665
Author(s):  
Marwa Louati ◽  
Blanca Salazar-Sarasua ◽  
Edelín Roque ◽  
José Pío Beltrán ◽  
Amel Salhi Hannachi ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Functional Characterization ◽  
Reproductive Development ◽  
Genetic Network ◽  
Floral Organ ◽  
Local Economy ◽  
Mads Box ◽  
Argania Spinosa ◽  
Mads Box Gene

Argan trees (Argania spinosa) belong to a species native to southwestern Morocco, playing an important role in the environment and local economy. Argan oil extracted from kernels has a unique composition and properties. Argan trees were introduced in Tunisia, where hundreds of trees can be found nowadays. In this study, we examined reproductive development in Argan trees from four sites in Tunisia and carried out the functional characterization of a floral homeotic gene in this non-model species. Despite the importance of reproductive development, nothing is known about the genetic network controlling flower development in Argania spinosa. Results obtained in several plant species established that floral organ development is mostly controlled by MADS-box genes and, in particular, APETALA3 (AP3) and PISTILLATA (PI) homologs are required for proper petal and stamen identity. Here, we describe the isolation and functional characterization of a MADS-box gene from Argania spinosa. Phylogenetic analyses showed strong homology with PI-like proteins, and the expression of the gene was found to be restricted to the second and third whorls. Functional homology with Arabidopsis PI was demonstrated by the ability of AsPI to confer petal and stamen identity when overexpressed in a pi-1 mutant background. The identification and characterization of this gene support the strong conservation of PI homologs among distant angiosperm plants.

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