scholarly journals Evaluation of In Vitro Solar Protection Factor (SPF), Antioxidant Activity, and Cell Viability of Mixed Vegetable Extracts from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 453 ◽  
Author(s):  
Letícia Caramori Cefali ◽  
Janaina Artem Ataide ◽  
Ana Rita Fernandes ◽  
Elena Sanchez-Lopez ◽  
Ilza Maria de Oliveira Sousa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitis Vinifera ◽  
Cell Viability ◽  
Ginkgo Biloba ◽  
Sun Protection ◽  
Hplc Method ◽  
Vitis Vinifera L ◽  
Ruta Graveolens ◽  
Protection Factor

The aim of this study was to validate a HPLC method for the assay of flavonoids in extracts obtained from natural sources, i.e., from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. The potential sun protecting effect, antioxidant activity, and cell viability of the extracts were also determined. Individual extracts (obtained from each individual species) and a mixed extract (containing the four extracts) were analyzed by the validated HPLC method for the identification of flavonoids and quantification of rutin and quercetin. An in vitro cell viability study was carried out using the neutral red method. The in vitro sun protection factor was determined by spectral transmittance and in vitro antioxidant efficacy was evaluated against DPPH, ABTS, and AAPH radicals. The HPLC method used for the identification and quantification of flavonoids in extracts exhibited linearity, precision, accuracy, and robustness. Detection and quantification limits were, respectively, 2.881 ± 0.9 μg·mL−1 and 0.864 ± 0.9 μg·mL−1 for quercetin, and 30.09 ± 1 μg·mL−1 and 9.027 ± 1.1 μg·mL−1 for rutin. All extracts did not affect cell viability at the evaluated concentration range and exhibited a sun protection effect and antioxidant activity. Among the evaluated extracts, Ginkgo biloba L. and the mixed extract depicted the most expressive antioxidant activity. The mixed extract exhibited sunscreen protection against ultraviolet A (UVA) and ultraviolet B (UVB) and a critical wavelength of 372.7 ± 0.1. Our results translate the enhanced flavonoids’ composition of the mixed extract, which may be a potential alternative over sunscreens and antioxidants in pharmaceutic/cosmetic formulations.

In vitro solar protection factor, antioxidant activity, and stability of a topical formulation containing Benitaka grape (Vitis vinifera L.) peel extract

2019 ◽  
Vol 34 (18) ◽  
pp. 2677-2682 ◽  
Author(s):  
Letícia Caramori Cefali ◽  
Janaína Artem Ataide ◽  
Ilza Maria de Oliveria Sousa ◽  
Mariana Cecchetto Figueiredo ◽  
Ana Lucia Tasca Gois Ruiz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Topical Formulation ◽  
Protection Factor ◽  
Peel Extract

scholarly journals Safety and Photoprotective Efficacy of a Sunscreen System Based on Grape Pomace (Vitis vinifera L.) Phenolics from Winemaking

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1148
Author(s):  
Alexandra A. Hübner ◽  
Fernanda D. Sarruf ◽  
Camila A. Oliveira ◽  
Alberto V. Neto ◽  
Dominique C. H. Fischer ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitis Vinifera ◽  
Grape Pomace ◽  
Vitis Vinifera L ◽  
Uvb Radiation ◽  
Uv Filters ◽  
Clinical Tests ◽  
Dpph Assay ◽  
Protection Factor

In winemaking, a large amount of grape pomace is produced that is rich in polyphenolics and highly beneficial for human health, as phenols are useful for skin ultraviolet (UV) protection. In this investigation, we evaluated the safety and clinical efficacy of a sunscreen system containing a grape pomace extract from Vitis vinifera L. as a bioactive ingredient. The recovery of phenolics in the waste was performed by percolation. Nine emulsions were developed using a factorial design and two were evaluated clinically: Formulation E, containing only UV filters (butylmethoxydibenzoyl methane, ethylhexyl methoxycinnamate and ethylhexyl dimethyl PABA), and F, with the extract at 10.0% w/w + UV filters. The antioxidant activity was determined by the DPPH assay and the in vitro efficacy was established by sun protection factor (SPF) measurements (Labsphere UV-2000S). Clinical tests were performed to determine safety (human repeated insult patch test) and to confirm efficacy (photoprotective effectiveness in participants). The results showed a synergistic effect between the sunscreen system and the extract on UVB protection and antioxidant activity. Both samples were considered safe. Formulation F was 20.59% more efficient in protecting skin against UVB radiation, taking approximately 21% more time to induce erythema compared to the extract-free sample.

Chemical composition, antioxidant, alpha-glucosidase inhibitory, anticholinesterase and photoprotective activities of the aerial parts of Schinus molle L.

2020 ◽  
Vol 16 ◽  
Author(s):  
Mustapha M. Bouhenna ◽  
Chawki Bensouici ◽  
Latifa Khattabi ◽  
Farid Chebrouk ◽  
Nabil Mameri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Sun Protection ◽  
Antidiabetic Activity ◽  
Protection Factor ◽  
Schinus Molle ◽  
Rp Hplc ◽  
Sun Protection Factor

Background: Schinus molle L. is a medicinal and aromatic plant widely used in folk medicine commonly found in Algeria and Mediterranean region. In the present work, we investigated the in vitro antioxidant, anti-cholinesterase, αglucosidase inhibitory and photoprotective potentials of the Schinus molle L. plant’s extract/fractions. The metabolite profile of Schinus molle L. was analyzed using RP-HPLC and GC-MS. Methods: Antioxidant activity was assessed using different tests. Anticholinesterase activity was investigated by quantifying the acetylcholinesterase and butyrylcholinesterase inhibitory activities. Antidiabetic activity was investigated by αglucosidase inhibition test, whereas photoprotective activity was evaluated by sun protection factor (SPF) using spectrophotometry UV-Vis. The extracts were then submitted to RP-HPLC–PDA and GC–MS analysis. Results: Ethyl acetate and buthanol fractions with high contents of total phenolics and flavonoids exhibited the strongest antioxidant activity. Ethyl acetate extract exhibited considerable enzyme inhibition potential on acetylcholinesterase, butyrylcholinesterase and α-glucosidase with percentages of inhibition of 99.08 ± 0.79 %, 100 % and 98.80 ± 0.18 % respectively. Furthermore, the ethyl acetate fraction showed high photoprotective activity with the sun protection factor (SPF) value = 38,26 ± 0.73. Three phenolic acids (gallic, tannic and vanillic acids) and five flavonoids (myricetin, apigenin, naringenin, rutin and quercetin) were identified with RP-HPLC–PDA. Conclusion: These findings suggest that Schinus molle L. may be useful in the development of an alternative agent for oxidative stress, Alzheimer’s disease, diabetes and could be used as a natural sunscreen in pharmaceutics or cosmetic formulations.

Effects of Elevated CO2on Grapevine (Vitis vinifera L.): Volatile Composition, Phenolic Content, and in Vitro Antioxidant Activity of Red Wine

2009 ◽  
Vol 57 (1) ◽  
pp. 265-273 ◽  
Author(s):  
Berta Gonçalves ◽  
Virgílio Falco ◽  
José Moutinho-Pereira ◽  
Eunice Bacelar ◽  
Francisco Peixoto ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitis Vinifera ◽  
Red Wine ◽  
Phenolic Content ◽  
Vitis Vinifera L ◽  
Volatile Composition ◽  
In Vitro Antioxidant Activity

scholarly journals Flavonoid-Enriched Plant-Extract-Loaded Emulsion: A Novel Phytocosmetic Sunscreen Formulation with Antioxidant Properties

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 443 ◽  
Author(s):  
Cefali ◽  
Ataide ◽  
Fernandes ◽  
Sousa ◽  
Gonçalves ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Rupture Strength ◽  
Skin Irritation ◽  
Antioxidant Properties ◽  
Sun Protection ◽  
Ultraviolet Rays ◽  
Tape Stripping ◽  
Protection Factor ◽  
Vitro Assay

The aim of this study was to develop a phytocosmetic sunscreen emulsion with antioxidant effect, containing a blend of flavonoid-enriched plant extracts. In vitro sun protection factor, antioxidant activity, skin irritation, photostability, cutaneous permeation, and retention of flavonoids were evaluated. Thermodynamically stable emulsions were obtained and tested for sensorial analysis after loading the blend of extracts. The selected emulsion was stable when stored at low temperatures (5 C), for which after 120 days the concentration of quercetin and rutin were above their limit of quantification, i.e., 2.8 ± 0.39 µg/mL and 30.39 ± 0.39 µg/mL, respectively. Spreadability, low rupture strength and adhesiveness were shown to be similar to a conventional topical product. Higher brittleness, pseudo-plastic, and viscoelastic behaviors were also recorded for the developed phytocosmetic sunscreen. The product presented a critical wavelength of 387.0 nm and ultraviolet rays A and B (UVA/UVB) rate of 0.78, confirming that the developed formulation shows capacity for UVA/UVB protection, protecting skin against damages caused by Ultraviolet (UV) radiation. Rutin was shown to permeate the skin barrier and was also quantified in the stratum corneum (3.27 ± 1.92 µg/mL) by tape stripping and retention test (114.68 ± 8.70 µg/mL). The developed flavonoid-enriched phytocosmetic was shown to be non-irritant to skin by an in vitro assay. Our results confirm the antioxidant activity, sun protection, and physical properties of the developed phytocosmetic for topical application.

scholarly journals Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 490
Author(s):  
Martin Sák ◽  
Ivana Dokupilová ◽  
Šarlota Kaňuková ◽  
Michaela Mrkvová ◽  
Daniel Mihálik ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vitis Vinifera ◽  
Cell Cultures ◽  
Trichoderma Viride ◽  
Vitis Vinifera L ◽  
Treated Plant ◽  
Abiotic Elicitors ◽  
In Vitro Cell Cultures ◽  
Red Grape

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

scholarly journals Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv. Syrah grape pomace extract

2019 ◽  
Vol 10 (4) ◽  
pp. 1856-1869 ◽  
Author(s):  
Joana R. Costa ◽  
Manuela Amorim ◽  
Ana Vilas-Boas ◽  
Renata V. Tonon ◽  
Lourdes M. C. Cabral ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Human Health ◽  
Grape Pomace ◽  
Vitis Vinifera L ◽  
Gastrointestinal Digestion ◽  
Gastrointestinal Health ◽  
Bioactive Properties

Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including in gastrointestinal health.

Sign in / Sign up

Export Citation Format

Share Document