scholarly journals Assessment of Genetic Diversity of the “Acquaviva Red Onion” (Allium cepa L.) Apulian Landrace

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 260 ◽  
Author(s):  
Luigi Ricciardi ◽  
Rosa Mazzeo ◽  
Angelo Raffaele Marcotrigiano ◽  
Guglielmo Rainaldi ◽  
Paolo Iovieno ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Allium Cepa ◽  
Germplasm Collection ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Vegetable Crop ◽  
Allium Cepa L ◽  
Soluble Solid ◽  
History Of

Onion (Allium cepa L.) is the second most important vegetable crop worldwide and is widely appreciated for its health benefits. Despite its significant economic importance and its value as functional food, onion has been poorly investigated with respect to its genetic diversity. Herein, we surveyed the genetic variation in the “Acquaviva red onion” (ARO), a landrace with a century-old history of cultivation in a small town in the province of Bari (Apulia, Southern of Italy). A set of 11 microsatellite markers were used to explore the genetic variation in a germplasm collection consisting of 13 ARO populations and three common commercial types. Analyses of genetic structure with parametric and non-parametric methods highlighted that the ARO represents a well-defined gene pool, clearly distinct from the Tropea and Montoro landraces with which it is often mistaken. In order to provide a description of bulbs, usually used for fresh consumption, soluble solid content and pungency were evaluated, showing higher sweetness in the ARO with respect to the two above mentioned landraces. Overall, the present study is useful for the future valorization of the ARO, which could be promoted through quality labels which could contribute to limit commercial frauds and improve the income of smallholders.

scholarly journals Morphological and Molecular Characterization of Some Tomato (Solanum lycopersicum L.) Genotypes Collected From Erzincan Province of Turkey

2022 ◽  
Author(s):  
HALIL IBRAHIM OZTURK
Keyword(s):  
Genetic Diversity ◽  
Fruit Weight ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Solanum Lycopersicum L ◽  
Soluble Solid ◽  
Polymorphism Rate ◽  
Tomato Genotypes ◽  
Local Farmers

Abstract BackroundTurkey is a country with different geographical features and therefore it is extremely diverse in plant diversity. Tomato is one of the most important vegetables produced both in the world and in Turkey. In this study, it was aimed to determine the genetic diversity of 24 tomato genotypes collected from local farmers from “Center villages” and “Üzümlü” district in Erzincan province.Methods and ResultsMorphological (qualitative and quantitative) and molecular markers (ISSR) were used to determine genetic diversity among genotypes. Genotype 24 was found to be higher than other genotypes with important quantitative morphological features such as fruit length, fruit width, fruit weight and soluble solid content (SSC). Considering the overall morphological traits, a wide variation was detected between genotypes. According to the molecular findings obtained. The polymorphism rate ranged from 0–100% and the average polymorphism rate was calculated as 80%.ConclusionPresent findings revealed the diversity in tomato genotypes collected from Erzincan province and may constitute the bases for further breeding studies in tomato and will bring an integrity in tomato identification studies.

scholarly journals Genetic Diversity within Chinese Watermelon Ecotypes Compared with Germplasm from Other Countries

2012 ◽  
Vol 137 (3) ◽  
pp. 144-151 ◽  
Author(s):  
Yunyan Sheng ◽  
Feishi Luan ◽  
Faxing Zhang ◽  
Angela R. Davis
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Citrullus Lanatus ◽  
Morphological Characteristics ◽  
Male Flower ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Similarity Coefficients ◽  
Branch Number ◽  
Soluble Solid

Genetic diversity among 95 watermelon (Citrullus lanatus) ecotypes was evaluated and compared with representative Chinese, American, Japanese, and Russian watermelon cultigens, landraces, and a wild watermelon relative (Trichosanthes kirilowii). Open-pollinated, hybrid, and inbred lines were included for most of the ecotypes and are hereafter collectively referred to as cultigens unless an ecotype group is being discussed. Morphological characteristics (including days to flower, female to male flower ratio, branch number, fruit length and diameter ratio, fruit soluble solid content, fruit yield, and simple sequence repeat (SSR) markers were used to estimate genetic diversity. Of 398 watermelon primer pairs tested, 9.5% (38) produced polymerase chain reaction amplicons in watermelon. Of these 38 primer pairs, the average number of polymorphic bands among the 96 cultigens was 2.4, even with 12 primer pairs demonstrating monomorphic banding patterns. Based on the SSR data, the genetic similarity coefficients were calculated and a dendrogram constructed. All cultigens were clustered to six groups. The wild species and landraces formed distant clusters from the cultivated watermelon. The genetic similarity coefficients within the Chinese cultigens ranged from 0.37 to 0.99, but except for a wild relative to watermelon, most cultigens were closely related. The genetic distance among non-Chinese cultigens ranged from 0.67 to 0.91 with an average of 0.88. When combined morphological traits and molecular traits were assessed, Russian and U.S. fruit were more genetically similar to each other than to Chinese and Japanese cultigens. Crossing Russian and/or U.S. cultigens with Chinese or Japanese cultigens should thus improve genetic diversity and introduce new traits for the resulting watermelon cultigens.

Genetic Analysis of Chinese Cucumber Collections in the U.S. National Germplasm Collection

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 534e-534 ◽  
Author(s):  
J. Staub ◽  
Felix Sequen ◽  
Tom Horejsi ◽  
Jin Feng Chen
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Genetic Variation ◽  
Genetic Analysis ◽  
Allelic Variation ◽  
Germplasm Collection ◽  
Principal Component ◽  
Isozyme Loci ◽  
National Plant Germplasm System ◽  
The U.S

Genetic variation in cucumber accessions from China was assessed by examining variation at 21 polymorphic isozyme loci. Principal component analysis of allelic variation allowed for the depiction of two distinct groupings of Chinese accessions collected in 1994 and 1996 (67 accessions). Six isozyme loci (Gpi, Gr, Mdh-2, Mpi-2, Pep-gl, and Pep-la) were important in elucidating these major groups. These groupings were different from a single grouping of Chinese 146 accessions acquired before 1994. Allelic variation in Chinese accessions allowed for comparisons with other accessions in the U.S. National Plant Germplasm System (U.S. NPGS) collection grouped by continent and sub-continent. When Chinese accessions taken collectively were compared with an array of 853 C. sativus U.S. NPGS accessions examined previously, relationships differed between accessions grouped by country or subcontinent. Data indicate that acquisition of additional Chinese and Indian cucumber accessions would be strategically important for increasing genetic diversity in the U.S. NPGS cucumber collection.

Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

2019 ◽  
Vol 14 (7) ◽  
pp. 649-657 ◽  
Author(s):  
Li Li ◽  
Jiemin Li ◽  
Jian Sun ◽  
Ping Yi ◽  
Changbao Li ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Significant Inhibition ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Pericarp Browning

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

scholarly journals Effect of Cross-Linked Alginate/Oil Nanoemulsion Coating on Cracking and Quality Parameters of Sweet Cherries

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 449
Author(s):  
Camilo Gutiérrez-Jara ◽  
Cristina Bilbao-Sainz ◽  
Tara McHugh ◽  
Bor-Sen Chiou ◽  
Tina Williams ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Quality Parameters ◽  
Solid Content ◽  
Postharvest Quality ◽  
Soluble Solid Content ◽  
Nutritional Values ◽  
Quality Properties ◽  
Vapor Transfer ◽  
Sweet Cherries ◽  
Soluble Solid

The cracking of sweet cherries causes significant crop losses. Sweet cherries (cv. Bing) were coated by electro-spraying with an edible nanoemulsion (NE) of alginate and soybean oil with or without a CaCl2 cross-linker to reduce cracking. Coated sweet cherries were stored at 4 °C for 28 d. The barrier and fruit quality properties and nutritional values of the coated cherries were evaluated and compared with those of uncoated sweet cherries. Sweet cherries coated with NE + CaCl2 increased cracking tolerance by 53% and increased firmness. However, coated sweet cherries exhibited a 10% increase in water loss after 28 d due to decreased resistance to water vapor transfer. Coated sweet cherries showed a higher soluble solid content, titratable acidity, antioxidant capacity, and total soluble phenolic content compared with uncoated sweet cherries. Therefore, the use of the NE + CaCl2 coating on sweet cherries can help reduce cracking and maintain their postharvest quality.

scholarly journals Genetic variation in native and farmed populations of Tambaqui (Colossoma macropomum) in the Brazilian Amazon: regional discrepancies in farming systems

2013 ◽  
Vol 85 (4) ◽  
pp. 1439-1447 ◽  
Author(s):  
Jonas Aguiar ◽  
Horacio Schneider ◽  
Fatima Gomes ◽  
Jeferson Carneiro ◽  
Simoni Santos ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Dna Sequences ◽  
Amazon Basin ◽  
Farming Systems ◽  
Wild Populations ◽  
The West ◽  
Colossoma Macropomum ◽  
Breeding Stock ◽  
History Of

The tambaqui, Colossoma macropomum, is the most popular fish species used for aquaculture in Brazil but there is no study comparing genetic variation among native and farmed populations of this species. In the present study, we analyzed DNA sequences of the mitochondrial DNA to evaluate the genetic diversity among two wild populations, a fry-producing breeding stock, and a sample of fish farm stocks, all from the region of Santarém, in the west of the Brazilian state of Pará. Similar levels of genetic diversity were found in all the samples and surprisingly the breeding stock showed expressive representation of the genetic diversity registered on wild populations. These results contrast considerably with those of the previous study of farmed stocks in the states of Amapá, Pará, Piauí, and Rondônia, which recorded only two haplotypes, indicating a long history of endogamy in the breeding stocks used to produce fry. The results of the two studies show two distinct scenarios of tambaqui farming in the Amazon basin, which must be better evaluated in order to guarantee the successful expansion of this activity in the region, and the rest of Brazil, given that the tambaqui and its hybrids are now farmed throughout the country.

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