scholarly journals Effect of Chitosan on the Activity of Water-Soluble and Hydrophobic Porphyrin Photosensitizers Solubilized by Amphiphilic Polymers

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1007
Author(s):  
Valeriya V. Kardumyan ◽  
Nadejda A. Aksenova ◽  
Victoria A. Timofeeva ◽  
Alexey V. Krivandin ◽  
Olga V. Shatalova ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Amphiphilic Polymers ◽  
Model Reaction ◽  
Amphiphilic Polymer ◽  
Water Soluble ◽  
Pluronic F127 ◽  
Polymer Systems ◽  
Photo Oxidation ◽  
Porphyrin Photosensitizers ◽  
Trisodium Salt

In this work, we studied the photocatalytic activity of photosensitizers (PSs) of various natures solubilized with polyvinylpyrrolidone (PVP) and ternary block copolymer ethylene and propylene oxide Pluronic F127 (F127) in a model reaction of tryptophan photo-oxidation in water in the presence of chitosan (CT). Water-soluble compounds (dimegin and trisodium salt of chlorin e6 (Ce6)) and hydrophobic porphyrins (tetraphenylporphyrin (TPP) and its fluorine derivative (TPPF20)) were used as PSs. It was shown that the use of chitosan (Mw ~100 kDa) makes it possible to obtain a system whose activity is comparable to that of the photosensitizer-amphiphilic polymer systems. Thus, the previously observed drop in the photosensitizing activity of PS in the presence of a polysaccharide and amphiphilic polymers (AP) was absent in this case. At the same time, chitosan had practically no inhibitory effect on hydrophobic porphyrins solubilized by Pluronic F127.

scholarly journals Ультрафиолетовая люминесценция и светорассеяние систем фотодитазина с альгинатом натрия, поли-N-винилпирролидоном и триптофаном-=SUP=-*-=/SUP=-

2019 ◽  
Vol 126 (6) ◽  
pp. 781
Author(s):  
Л.В. Беловолова ◽  
М.В. Глушков ◽  
Н.А. Аксенова ◽  
А.Б. Соловьева ◽  
О.В. Хасанова
Keyword(s):  
Ternary Complex ◽  
Rayleigh Scattering ◽  
Mutual Influence ◽  
Water Soluble ◽  
Polymer Systems ◽  
Fluorescence Parameters ◽  
Photo Oxidation ◽  
Oxidation Processes ◽  
Preparation Conditions ◽  
Scattering Spectra

As part of the study of polymer systems based on a chlorine photosensitizer, photodithazine (PD), photodynamic therapy was used to study the fluorescence and Rayleigh scattering spectra upon excitation at wavelengths lambdaex = 260, 280, 400, and 450 nm. Non-toxic water-soluble poly-N-vinylpyrrolidone (PVP) and vegetable polysaccharide were used as the polymer. sodium alginate (SA). The data of the PD-SA dual system is characterized by variability of characteristics depending on the preparation conditions, while in the PD-SA-PVP ternary complex, there is a favorable mutual influence of two SA and PVP polymers on the PD molecule, due to the creation of a local microenvironment of the active center PD with increased polarity . It was shown that the introduction of tryptophan (Trp), used as a substrate in model photo-oxidation processes, when testing the effectiveness of photosensitizers in the generation of singlet oxygen, does not significantly change the fluorescence parameters of the PD-SA-PVP complex.

The influence of amphiphilic polymers on the photocatalytic activity of water-soluble porphyrin photosensitizers

2011 ◽  
Vol 85 (5) ◽  
pp. 871-875 ◽  
Author(s):  
Yu. A. Gorokh ◽  
N. A. Aksenova ◽  
A. B. Solov’eva ◽  
V. A. Ol’shevskaya ◽  
A. V. Zaitsev ◽  
...  
Keyword(s):  
Photocatalytic Activity ◽  
Amphiphilic Polymers ◽  
Water Soluble ◽  
Porphyrin Photosensitizers ◽  
Water Soluble Porphyrin

Polymers as enhancers of photodynamic activity of chlorin photosensitizers for photodynamic therapy

2013 ◽  
Vol 2 (3) ◽  
Author(s):  
Nadezhda A. Aksenova ◽  
Timur M. Zhientaev ◽  
Anna A. Brilkina ◽  
Ljubov V. Dubasova ◽  
Andrey V. Ivanov ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Dosage Form ◽  
Considerable Increase ◽  
Model Reaction ◽  
Water Soluble ◽  
Effective Dosage ◽  
Polymer Systems ◽  
Photodynamic Activity ◽  
Polymer Interactions ◽  
The Impact

Abstract:The impact of water-soluble and amphiphilic polymers with different structures, namely carboxymethyl cellulose (CMC), polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP), was studied on the photoactivity of chlorin photosensitizers (PSs) in photodynamic therapy (PDT). It was shown that such polymers can cause a considerable increase in the PS activity, both in the process of singlet oxygen photogeneration in cell experiments, and in the model reaction of a substrate photooxidation in water. Amongst the studied polymers, CMC and PVP appeared to have the most significant influence on the photoactivity of PSs. The observed effect of the polymers on the photosensitizing activity of PSs can be attributed to the presence of chlorin-polymer interactions resulting in the porphyrin disaggregation in aqueous phase. The effect of the polymers on the photocytotoxicity of PSs is attributed to the absence of interactions between chlorin and polypeptide or lipoproteins which results in a decrease of the photoactivity of chlorins in cell culture. The PS/polymer systems appear to be a new effective dosage form of PDT drugs.

scholarly journals Nano-Bio Interaction between Blood Plasma Proteins and Water-Soluble Silicon Quantum Dots with Enabled Cellular Uptake and Minimal Cytotoxicity

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2250
Author(s):  
Shanmugavel Chinnathambi ◽  
Nobutaka Hanagata ◽  
Tomohiko Yamazaki ◽  
Naoto Shirahata
Keyword(s):  
Quantum Dots ◽  
Blood Plasma ◽  
Cellular Uptake ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Water Soluble ◽  
Pluronic F127 ◽  
Infrared Light ◽  
Spectral Window ◽  
Blood Plasma Proteins

A better understanding of the compatibility of water-soluble semiconductor quantum dots (QDs) upon contact with the bloodstream is important for biological applications, including biomarkers working in the first therapeutic spectral window for deep tissue imaging. Herein, we investigated the conformational changes of blood plasma proteins during the interaction with near-infrared light-emitting nanoparticles, consisting of Pluronic F127 shells and cores comprised of assembled silicon QDs terminated with decane monolayers. Albumin and transferrin have high quenching constants and form a hard protein corona on the nanoparticle. In contrast, fibrinogen has low quenching constants and forms a soft protein corona. A circular dichroism (CD) spectrometric study investigates changes in the protein’s secondary and tertiary structures with incremental changes in the nanoparticle concentrations. As expected, the addition of nanoparticles causes the denaturation of the plasma proteins. However, it is noteworthy that the conformational recovery phenomena are observed for fibrinogen and transferrin, suggesting that the nanoparticle does not influence the ordered structure of proteins in the bloodstream. In addition, we observed enabled cellular uptake (NIH3T3 Fibroblasts) and minimal cytotoxicity using different cell lines (HeLa, A549, and NIH3T3). This study offers a basis to design QDs without altering the biomacromolecule’s original conformation with enabled cellular uptake with minimal cytotoxicity.

CHEMICAL CONSTITUENTS IN WILD OAT (Avenu fatua) HULLS AND THEIR EFFECTS ON SEED GERMINATION

1982 ◽  
Vol 62 (1) ◽  
pp. 155-161 ◽  
Author(s):  
FRANK S. CHEN ◽  
JOHN M. MacTAGGART ◽  
RICHARD M. ELOFSON
Keyword(s):  
Seed Germination ◽  
Chemical Constituents ◽  
Short Chain Fatty Acids ◽  
Inhibitory Effect ◽  
Water Soluble ◽  
Nonanoic Acid ◽  
Lettuce Seed ◽  
Wild Oat ◽  
Phenolic Inhibitor ◽  
Lettuce Seed Germination

Aqueous extracts of dormant wild oat (Avena fatua L.) hulls had a weak inhibitory effect on lettuce seed germination. Both paper and thin layer chromatographic analyses of the ether-soluble acidic fraction showed the presence of phenolics and short-chain fatty acids. No abscisic acid was detected. Vanillin, protochatechualdehyde, p-coumaric acid, ferulic acid and caffeic acid were isolated from hull extracts. Vanillin was shown to be the main water-soluble phenolic inhibitor of lettuce seed germination. At a concentration higher than 6.6 × 10−3M, vanillin was found to be more inhibitory than nonanoic acid to both lettuce and wild oat germination. The concentration of phenolics in the hulls does not account for suppression of wild oat seed germination.

The mechanism of perylene photo-oxidation in a water-soluble polymeric photocatalyst

1996 ◽  
Vol 100 (1-3) ◽  
pp. 93-100 ◽  
Author(s):  
Nicholas A.D. Burke ◽  
Markus Templin ◽  
James E. Guillet
Keyword(s):  
Water Soluble ◽  
Photo Oxidation

scholarly journals Anti-Tumor Effects of Sodium Meta-Arsenite in Glioblastoma Cells with Higher Akt Activities

2020 ◽  
Vol 21 (23) ◽  
pp. 8982
Author(s):  
Eun Jeong Lee ◽  
Jee Young Sung ◽  
Kyung Hee Koo ◽  
Jong Bae Park ◽  
Dae Hong Kim ◽  
...  
Keyword(s):  
Cell Lines ◽  
Proteasomal Degradation ◽  
Inhibitory Effect ◽  
Water Soluble ◽  
Breast Cancer Cell Lines ◽  
Normal Brain ◽  
Oncogenic Signaling ◽  
Pten Loss ◽  
Akt Activation

Glioblastoma is a type of aggressive brain tumor that grows very fast and evades surrounding normal brain, lead to treatment failure. Glioblastomas are associated with Akt activation due to somatic alterations in PI3 kinase/Akt pathway and/or PTEN tumor suppressor. Sodium meta-arsenite, KML001 is an orally bioavailable, water-soluble, and trivalent arsenical and it shows antitumoral effects in several solid tumor cells via inhibiting oncogenic signaling, including Akt and MAPK. Here, we evaluated the effect of sodium meta-arsenite, KML001, on the growth of human glioblastoma cell lines with different PTEN expression status and Akt activation, including PTEN-deficient cells (U87-MG and U251) and PTEN-positive cells (LN229). The growth-inhibitory effect of KML001 was stronger in U87-MG and U251 cells, which exhibited higher Akt activity than LN229 cells. KML001 deactivated Akt and decreased its protein levels via proteasomal degradation in U87-MG cells. KML001 upregulated mutant PTEN levels via inhibition of its proteasomal degradation. KML001 inhibited cell growth more effectively in active Akt-overexpressing LN229 cells than in mock-expressing LN229 cells. Consistent with these results, KML001 sensitized PTEN-deficient cells more strongly to growth inhibition than it did PTEN-positive cells in prostate and breast cancer cell lines. Finally, we illustrated in vivo anti-tumor effects of KML001 using an intracranial xenograft mouse model. These results suggest that KML001 could be an effective chemotherapeutic drug for the treatment of glioblastoma cancer patients with higher Akt activity and PTEN loss.

scholarly journals Supramolecular tripodal Au(i) assemblies in water. Interactions with a pyrene fluorescent probe

2019 ◽  
Vol 43 (21) ◽  
pp. 8279-8289 ◽  
Author(s):  
Andrea Pinto ◽  
Guillem Hernández ◽  
Raquel Gavara ◽  
Elisabet Aguiló ◽  
Artur J. Moro ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Water Soluble ◽  
Water Soluble Phosphines ◽  
Trisodium Salt

The synthesis of three gold(i) tripodal complexes derived from tripropargylamine and containing the water soluble phosphines PTA (1,3,5-triaza-7-phosphaadamantane), DAPTA (3,7-diacetyl-1,3,7-triaza-5-phosphabicyclo[3.3.1]nonane) and TPPTS (triphenylphosfine-3,3′,3′′-trisulfonic acid trisodium salt) is described here.

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