scholarly journals Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2929
Author(s):  
Jing Miao ◽  
Liwen Zhang ◽  
Peng Gao ◽  
Huawei Zhao ◽  
Xianji Xie ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Glucose Transporter ◽  
Malignant Tumors ◽  
Sirna Delivery ◽  
Survival Rates ◽  
Chitosan Oligosaccharide ◽  
Glucose Transporter 1 ◽  
Glut 1 ◽  
Intrinsic Marker ◽  
Sirna Delivery System

Glucose Transporter-1 (GLUT-1) is considered to be a possible intrinsic marker of hypoxia in malignant tumors, which is an important factor in radioresistance of laryngocarcinoma. We speculated that the inhibition of GLUT-1 expression might improve the radiosensitivity of laryngocarcinoma. GLUT-1 siRNA was designed to inhibit the GLUT-1 expression, but the high molecular weight and difficult drug delivery limited the application. Herein, we constructed a glycolipid polymer chitosan oligosaccharide grafted stearic acid (CSSA) to conjugate siRNA via electrostatic interaction. The characteristics of CSSA and CSSA/siRNA were studied, as well as the radiosensitization effect of siRNA on human laryngocarcinoma epithelial (Hep-2) cells. Compared with the traditional commercial vector LipofectamineTM2000 (Lipo), CSSA exhibited lower cytotoxicity, more efficiently cellular uptake. Incubating with CSSA/siRNA, the survival rates of Hep-2 cells were significantly decreased comparing with either the group before transfection or Lipo/siRNA. CSSA is a promising carrier for efficient siRNA delivery and radiosensitization of laryngocarcinoma.

Differential glycolytic and glycogenogenic transduction pathways in male and female bovine embryos produced in vitro

2012 ◽  
Vol 24 (2) ◽  
pp. 344 ◽  
Author(s):  
M. Garcia-Herreros ◽  
I. M. Aparicio ◽  
D. Rath ◽  
T. Fair ◽  
P. Lonergan
Keyword(s):  
Glucose Metabolism ◽  
Protein Expression ◽  
Glucose Uptake ◽  
Glucose Transporter ◽  
Glycogen Synthase ◽  
Bovine Embryos ◽  
High Concentration ◽  
Glucose Transporter 1 ◽  
Male And Female ◽  
Glut 1

Previous studies have shown that developmental kinetic rates following IVF are lower in female than in male blastocysts and that this may be related to differences in glucose metabolism. In addition, an inhibition of phosphatidylinositol 3-kinase (PI3-K) inhibits glucose uptake in murine blastocysts. Therefore, the aim of this study was to identify and compare the expression of proteins involved in glucose metabolism (hexokinase-I, HK-I; phosphofructokinase-1, PFK-1; pyruvate kinase1/2, PK1/2; glyceraldehyde-3-phosphate dehydrogenase, GAPDH; glucose transporter-1, GLUT-1; and glycogen synthase kinase-3, GSK-3) in male and female bovine blastocysts to determine whether PI3-K has a role in the regulation of the expression of these proteins. Hexokinase-I, PFK-1, PK1/2, GAPDH and GLUT-1 were present in bovine embryos. Protein expression of these proteins and GSK-3 was significantly higher in male compared with female blastocysts. Inhibition of PI3-K with LY294002 significantly decreased the expression of HK-I, PFK-1, GAPDH, GSK-3 A/B and GLUT-1. Results showed that the expression of glycolytic proteins HK-I, PFK-1, GAPDH and PK1/2, and the transporters GLUT-1 and GSK-3 is regulated by PI3-K in bovine blastocysts. Moreover, the differential protein expression observed between male and female blastocysts might explain the faster developmental kinetics seen in males, as the expression of main proteins involved in glycolysis and glycogenogenesis was significantly higher in male than female bovine embryos and also could explain the sensitivity of male embryos to a high concentration of glucose, as a positive correlation between GLUT-1 expression and glucose uptake in embryos has been demonstrated.

Overexpression of Glucose Transporter-1 (GLUT-1) Predicts Poor Prognosis in Epithelial Ovarian Cancer

2013 ◽  
Vol 31 (9) ◽  
pp. 607-615 ◽  
Author(s):  
Hanbyoul Cho ◽  
You Sun Lee ◽  
Julie Kim ◽  
Joon-Yong Chung ◽  
Jae-Hoon Kim
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Poor Prognosis ◽  
Glucose Transporter ◽  
Glucose Transporter 1 ◽  
Glut 1

scholarly journals Expression of Glucose Transporter 1 Confers Susceptibility to Human T-Cell Leukemia Virus Envelope-Mediated Fusion

2005 ◽  
Vol 79 (7) ◽  
pp. 4150-4158 ◽  
Author(s):  
Ayse Kubra Coskun ◽  
Richard E. Sutton
Keyword(s):  
T Cell ◽  
Glucose Transporter ◽  
Leukemia Virus ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Glucose Transporter 1 ◽  
Glut 1 ◽  
Human T Cell ◽  
T Cell Leukemia Virus ◽  
Mdbk Cells

ABSTRACT Human T-cell leukemia virus type 1 (HTLV-1) was the first human retrovirus identified and causes both adult T-cell leukemia/lymphoma and tropical spastic paraparesis/HTLV-1-associated myelopathy, among other disorders. In vitro, HTLV-1 has an extremely broad host cell tropism in that it is capable of infecting most mammalian cell types, although at the same time viral titers remain relatively low. Despite years of study, only recently has a bona fide candidate cellular receptor, glucose transporter 1 (glut-1), been identified. Although glut-1 was shown to bind specifically to the ectodomain of HTLV-1 and HTLV-2 envelope glycoproteins, which was reversible with small interfering RNA directed against glut-1, cellular susceptibility to HTLV upon expression of glut-1 was not established. Here we show that expression of glut-1 in relatively resistant MDBK cells conferred increased susceptibility to both HTLV-1- and HTLV-2-pseudotyped particles. glut-1 also markedly increased syncytium formation in MDBK cells after exposure to HTLV-1. Another assay also demonstrated HTLV-1 envelope-cell fusion in the presence of glut-1. Taken together, these results provide additional evidence that glut-1 is a receptor for HTLV.

scholarly journals Immunohistochemical Markers for Prospective Studies in Neurofibromatosis-1 Porcine Models

2017 ◽  
Vol 65 (10) ◽  
pp. 607-618 ◽  
Author(s):  
David K. Meyerholz ◽  
Georgina K. Ofori-Amanfo ◽  
Mariah R. Leidinger ◽  
J. Adam Goeken ◽  
Rajesh Khanna ◽  
...  
Keyword(s):  
Glucose Transporter ◽  
Malignant Tumors ◽  
Neurofibromatosis Type ◽  
Nuclear Antigen ◽  
Inflammatory Factor ◽  
Glucose Transporter 1 ◽  
Peripheral Nerve Sheath Tumors ◽  
Immunohistochemical Markers ◽  
Increased Risk ◽  
Calbindin D

Neurofibromatosis type 1 (NF1) is a common, cancer-predisposing disease caused by mutations in the NF1 tumor gene. Patients with NF1 have an increased risk for benign and malignant tumors of the nervous system (e.g., neurofibromas, malignant peripheral nerve sheath tumors, gliomas) and other tissues (e.g., leukemias, rhabdomyosarcoma, etc.) as well as increased susceptibility to learning disabilities, chronic pain/migraines, hypertension, pigmentary changes, and developmental lesions (e.g., tibial pseudoarthrosis). Pigs are an attractive and upcoming animal model for future NF1 studies, but a potential limitation to porcine model research has been the lack of validated reagents for direct translational study to humans. To address that issue, we used formalin-fixed tissues (human and pigs) to evaluate select immunohistochemical markers (activated caspase-3, allograft inflammatory factor-1, beta-tubulin III, calbindin D, CD13, CD20, desmin, epithelial membrane antigen, glial fibrillary acidic protein, glucose transporter-1, laminin, myelin basic protein, myoglobin, proliferating cell nuclear antigen, S100, vimentin, and von Willebrand factor). The markers were validated by comparing known expression and localization in human and pig tissues. Validation of these markers on fixed tissues will facilitate prospective immunohistochemical studies of NF1 pigs, as well as other pig models, in a more efficient, reproducible, and translationally relevant manner.

scholarly journals Expression of glucose transporter 1 (GLUT 1) in the epithelial layer of an ex vivo produced human oral mucosa equivalent.

2001 ◽  
Vol 47 (5) ◽  
pp. 289-292 ◽  
Author(s):  
Kenji IZUMI ◽  
Hiroto TERASHI ◽  
Michiko YOSHIZAWA ◽  
Cynthia L. MARCELO ◽  
Stephen E. FEINBERG
Keyword(s):  
Oral Mucosa ◽  
Glucose Transporter ◽  
Ex Vivo ◽  
Epithelial Layer ◽  
Glucose Transporter 1 ◽  
Glut 1
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