In vitro ruminal undegradable proteins of alfalfa cultivars

The quality of alfalfa would be greatly improved by an increase in its ruminal undegradable protein (RUP) concentration. Protein degradation rate (PDR), in vitro dry matter digestibility (IVDMD), leaf weight ratio (LWR), dry matter yield (DMY), total nitrogen (TN), in vitro RUP (expressed on both TN, RUP-TN, and dry matter basis, RUP-DM), acid detergent fiber (ADF), and neutral detergent fiber (NDF) concentrations were determined in 27 alfalfa cultivars. Cultivars were seeded in triplicate on 2 consecutive years and evaluated during the 2 subsequent production years with two harvests per year. Protein degradation rate and RUP-TN were determined using a ruminal inhibitor in vitro system. Data were averaged for spring growth, summer regrowth, and both harvests across 2 production years. Each of the three data sets was analyzed by ANOVA followed by a principal component analysis (PCA) on the ANOVA means. For the four-harvest data, cultivar differences were highly significant (P < 0.001) for all variates except for PDR (P = 0.07) and RUP-TN concentration (P = 0.10). The first PCA axis was largely defined positively by RUP-DM, IVDMD, TN, LWR, and RUP-TN, but negatively with ADF, NDF, PDR, and DMY. The second PCA axis defined a contrast between PDR versus RUP-TN, DMY, ADF, and NDF. Five cultivars were distinctive with high or low PCA scores in all three PCA. Rangelander and Heinrichs, along with Ultra, had low PDR; the first two cultivars had low DMY whereas Ultra was a medium-yielding cultivar. In contrast, Algonquin and Oneida VR had high PDR and medium DMY. While the first principal component (PC) indicated a general trend that low PDR and high RUP were associated with low-yielding cultivars, the second PC identified specific cultivars with both low PDR and high DMY. Therefore, selection for low PDR and high DMY is feasible. Key words: ruminal protein escape, dry matter digestibility, alfalfa

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Leaf and stem dry matter digestibility and ruminal undegradable proteins of alfalfa cultivars

Canadian Journal of Plant Science ◽

10.4141/p01-122 ◽

2002 ◽

Vol 82 (2) ◽

pp. 383-393 ◽

Cited By ~ 13

Author(s):

G. F. Tremblay ◽

G. Bélanger ◽

K. B. McRae ◽

R. Michaud

Keyword(s):

Dry Matter ◽

Nutritive Value ◽

Weight Ratio ◽

Cultivar Differences ◽

Eastern Canada ◽

Alfalfa Plant ◽

Dry Matter Digestibility ◽

Whole Plant ◽

Whole Plants

Increased dry matter digestibility and ruminal undegradable protein (RUP) concentration are traits highly ranked to improve the nutritive value of forage legumes. Whole-plant, leaf, and stem samples of 27 alfalfa cultivars grown in eastern Canada were analysed for total nitrogen (TN), in vitro RUP, and in vitro dry matter digestibility (IVDMD). The objectives were to determine if significant variation exists among these cultivars, and to establish the relationship between nutritive value parameters of leaves and stems, and those of whole plants. Cultivars were seeded in triplicate on 2 consecutive years and evaluated during the 2 subsequent production years with two harvests per year. There were significant differences among cultivars in IVDMD of stems and plants in spring growth and summer regrowth. Cultivar differences in leaf IVDMD were significant only in spring growth, but this variation was negligible in practical terms. Cultivars with high forage IVDMD also had high stem IVDMD and this relationship was stronger in summer regrowth. Alfalfa plant IVDMD is a function of stem digestibility and the leaf weight ratio (LWR). Cultivar differences were significant for plant TN in spring growth and summer regrowth, and for stem TN in spring growth only. Leaf (270 to 306 g kg-1 TN) and stem (242 to 287 g kg-1 TN) RUP concentrations varied among cultivars in spring growth. Cultivars with high stem RUP also had high leaf RUP concentration but differences in plant RUP were not always attributed to leaf and stem RUP. The RUP concentration was on average 10% higher in leaves than in stems in spring growth, and this difference reached 15% in summer regrowth. The LWR varied among cultivars in spring growth and summer regrowth, and it was positively related to plant RUP in spring growth. Improved whole-plant RUP concentration is unlikely to be achieved by simply selecting for high RUP in leaves or stems; LWR has a principal role in determining alfalfa plant RUP concentration. Key words: Dry matter digestibility, ruminal undegradable proteins, alfalfa, genetic variation, stems, leaves

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Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽

10.3390/pr9050879 ◽

2021 ◽

Vol 9 (5) ◽

pp. 879

Author(s):

Seong-Shin Lee ◽

Jeong-Seok Choi ◽

Dimas Hand Vidya Paradhipta ◽

Young-Ho Joo ◽

Hyuk-Jun Lee ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Dry Matter ◽

Lactobacillus Brevis ◽

Neutral Detergent Fiber ◽

Dry Matter Digestibility ◽

Fiber Digestibility ◽

Aerobic Stability ◽

Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

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EFFECT OF STRUCTURAL AND CHEMICAL FACTORS OF FORAGES ON POTENTIALLY DIGESTIBLE FIBER, INTAKE, AND TRUE DIGESTIBILITY BY RUMINANTS

Canadian Journal of Animal Science ◽

10.4141/cjas88-088 ◽

1988 ◽

Vol 68 (3) ◽

pp. 787-799 ◽

Cited By ~ 13

Author(s):

V. GIRARD ◽

G. DUPUIS

Keyword(s):

Cell Wall ◽

Dry Matter ◽

Plant Cell Wall ◽

Neutral Detergent Fiber ◽

Optimum Number ◽

Apparent Digestibility ◽

Cool Season ◽

Dry Matter Digestibility ◽

True Digestibility

In view of the large variation found in plant cell wall digestibilities with ruminants, an attempt was made to group 124 feeds into different lignification classes (clusters) on the basis of chemical characteristics. Each feed cluster was described using a structural coefficient [Formula: see text] that related the potentially digestible fiber (PDF, %) to the ratio between lignin and cell wall volume. The optimum number of clusters was determined iteratively by performing a regression of the apparent digestibility of dry matter at maintenance level (DDM1, %) against the PDF and cell soluble (SOL, %) contents of feeds. The [Formula: see text] coefficients varied from 0.05 (grains, N = 13) to 1.85 (corn silage, N = 3) and increased with the maturity of the grasses from 0.88 (legumes, vegetative cool season grasses, N = 26) to 1.33 (mature, cool season grasses, N = 19). Predicted PDF were closely correlated (r > 0.9, P < 0.01) to in vitro cell wall disappearances (IVCWD). Apparently digestible cell wall in four grasses and four legumes increased linearly with 96-h IVCWD and standard error (SE) was similar to the SE of predicted apparent digestible SOL from SOL concentrations. Assuming that similarity between SE could be also observed in larger samples, PDF and SOL were used in summative equations to predict apparent dry matter digestibility. DDM1 discounted for intake (DDM1 – 4, %) was regressed against SOL and PDF concentrations of 87 feeds:[Formula: see text]with ds and df, the true digestibilities of SOL and PDF. Estimates of ds and df were 0.98 and 0.95 for a zero-production (maintenance) level of intake, and 0.91 and 0.79 for an intake level four times maintenance. Since the true digestibility of the PDF component was only 4% – 13% lower than that of the cell soluble component, the concentration of PDF in cell wall was the major determinant in the variation in apparent digestibility of forages. Key words: lignin, neutral detergent fiber, true digestibility, cluster analysis, feeds

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Production and quality of Tifton 85 pastures overseeded with black oat: effects of irrigation and nitrogen fertilization

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n5p2071 ◽

2018 ◽

Vol 39 (5) ◽

pp. 2071

Author(s):

João De Assis Farias Filho ◽

Fabiana Luiza Matielo de Paula ◽

Adalberto Luiz de Paula ◽

Wagner Paris ◽

Fabrício Ghinzelli ◽

...

Keyword(s):

Nitrogen Fertilization ◽

Dry Matter ◽

Irrigation System ◽

Neutral Detergent Fiber ◽

Lower Percentage ◽

Forage Production ◽

Dry Matter Digestibility ◽

Randomized Design ◽

Dead Material

The objective of this work was to evaluate the effects of irrigation and nitrogen fertilization on the bromatological quality, forage production, and botanical and structural composition of Tifton 85 (Cynodon sp.) pastures overseeded with black oat (Avena strigosa). Four treatments were evaluated in a 2 × 2 factorial scheme (irrigated and non-irrigated × fertilized and non-fertilized), with three replicates, in a completely randomized design. In irrigated paddocks, the irrigation system was activated when the soil matric potential reached a value equal to, or higher than, 10 kPa and 135 kg N ha-1 was applied to fertilized paddocks, divided into four applications. The forage mass pre- and post-grazing, total forage production, and the botanical, structural, and bromatological composition of the pastures were evaluated. No interaction was observed between the irrigation and nitrogen fertilization factors for any of the variables and no significant differences were observed in forage mass between pre- and post-grazing or in Tifton leaf and stem percentages. Nitrogen fertilization had a significant effect on forage production, which was 2626.41 kg dry matter (DM) ha-1 higher in fertilized pastures than in unfertilized pastures. In addition, fertilization resulted in a lower percentage of dead material (6.66%), higher percentage of oat leaves (30.84%), higher leaf:stem ratio (1:45), higher crude protein content (24.13%), lower levels of neutral detergent fiber (NDF) (64.57%) and acid detergent fiber (ADF) (32.86%), and higher in vitro dry matter digestibility (73.01%) than in unfertilized pastures. The use of irrigation did not influence total forage production, however, it resulted in pastures with lower NDF (65.97%) and ADF (33.54%), and higher in vitro dry matter digestibility (73.48%) than unirrigated pastures. Nitrogen fertilization produced improvements in pasture structure, associated with higher dry matter yield and bromatological quality, while irrigation only produced pastures with lower fiber content and greater digestibility.

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Nutritional diversity of Brachiaria ruziziensis clones

Ciência Rural ◽

10.1590/0103-8478cr20160855 ◽

2018 ◽

Vol 48 (2) ◽

Author(s):

Ellen de Almeida Moreira ◽

Shirley Motta de Souza ◽

Alexandre Lima Ferreira ◽

Thierry Ribeiro Tomich ◽

José Augusto Gomes Azevêdo ◽

...

Keyword(s):

Degradation Rate ◽

Neutral Detergent Fiber ◽

Ruminal Fermentation ◽

Dry Matter Digestibility ◽

Brachiaria Ruziziensis ◽

Hierarchical Grouping ◽

Degradation Rates ◽

Main Components ◽

Lower Contribution

ABSTRACT: The aim of this study was to evaluate the nutritional diversity of Brachiaria ruziziensis clones through chemical composition and in vitro kinetics of ruminal fermentation. Twenty three clones of Brachiaria ruziziensis were used (15, 16, 46, 174, 411, 590, 651, 670, 768, 776, 844, 859, 950, 965, 970, 975, 1067, 1093, 1296, 1765, 1806, 1894 and 1972) and Brachiaria ruziziensis cv. ‘Kennedy’, Brachiaria brizantha cv. ‘Marandu’ and Brachiaria decumbens cv. ‘Basilisk’ as controls within 27 days of harvesting. The experimental design used randomized blocks with 26 treatments (genotypes) and three replications. Evaluation of the nutritional divergence was performed using principal components analysis, based on the following discriminatory variables: in vitro dry matter digestibility (IVDMD), neutral detergent fiber (NDF), lignin, crude protein (CP), degradation rate of non-fibrous carbohydrates (KdNFC) and degradation rate of fibrous carbohydrates (KdFC). The evaluation of the nutritional diversity of Brachiaria genotypes was based on the two main components (IVDMD and NDF), which explains 96.2% of the total variance Variables of lower contribution to the discrimination of the clones were as degradation rates of the fibrous and non-fibrous carbohydrates. In the agglomerative hierarchical grouping analysis, five distinct groups were identified, where V group, formed by clones 46, 768 and 1067 have higher values of IVDMD compared to the other clones.

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Yield and nutritional value of BRS Capiaçu grass at different regrowth ages

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n5p2045 ◽

2019 ◽

Vol 40 (5) ◽

pp. 2045 ◽

Cited By ~ 1

Author(s):

Flávio Pinto Monção ◽

Marco Aurélio Moraes Soares Costa ◽

João Paulo Sampaio Rigueria ◽

Marielly Maria Almeida Moura ◽

Vicente Ribeiro Rocha Júnior ◽

...

Keyword(s):

Dry Matter ◽

Northern Region ◽

Matter Content ◽

Neutral Detergent Fiber ◽

Pennisetum Purpureum ◽

In Vitro Digestibility ◽

Dry Matter Content ◽

Elephant Grass ◽

Dry Matter Digestibility

The objective was to evaluate the productivity, chemical composition, ruminal degradability of dry matter and digestibility of BRS capiaçu grass (Pennisetum purpureum Schum.) managed at five regrowth ages. A completely randomized design with ten replications was used in the BRS capiaçu elephant grass subjected to five cutting intervals (30, 60, 90, 120 and 150 days) in the summer, making a total of 50 plots with a useful area of 4 x 2 m. There were daily increases in the dry matter production in the order of 382 kg ha-1, with 49,859 kg ha-1 being produced at 150 regrowth days. The dry matter content and organic matter linearly increased (P < 0.01) at different regrowth ages. The crude protein content, in vitro dry matter digestibility and in vitro digestibility of neutral detergent fiber linearly reduced (P < 0.01) 0.037%, 0.196% and 0.256% per day, respectively. Potential degradability of dry matter decreased from 68.9% at 30 days to 44.7% at 150 regrowth days (0.194 percentage units per day). The rate of degradation of fraction B 'c' was not modified (P = 0.94), averaging 1.46% hour-1. In the cultivation of BRS capiaçu elephant grass in the summer season, in the northern region of Minas Gerais, the age for harvesting between 90 and 120 days of regrowth is recommended.

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Chemical characteristics, aerobic stability, and microbiological counts in corn silage re-ensiled with bacterial inoculant

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2018000900008 ◽

2018 ◽

Vol 53 (9) ◽

pp. 1045-1052

Author(s):

Mateus Merlo Coelho ◽

Lúcio Carlos Gonçalves ◽

José Avelino Santos Rodrigues ◽

Kelly Moura Keller ◽

Gustavo Vinícius de Souza dos Anjos ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Nutritive Value ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Dry Matter Digestibility ◽

Aerobic Stability ◽

Whole Plant

Abstract: The objective of this work was to evaluate the effects of re-ensiling and bacterial inoculation on the quality of corn silage. The experiment was carried out in a 2x2 factorial design with or without inoculant (association of Lactobacillus plantarum and Propionibacterium acidipropionici), and with re-ensiling after 36 hours of aerobic exposure or only ensiling of the whole plant of 'BRS 1055' corn. The fermentative quality, nutritional parameters, dry matter losses, aerobic stability, and microbiological counts of silages were evaluated. Re-ensiling caused an increase of pH and in acetic acid and propionic acid concentrations, as well as in the dry matter (DM), crude protein, neutral detergent fiber, and neutral detergent fiber crude protein contents. Conversely, there was a reduction in the nonfiber carbohydrates concentration and in in vitro dry matter digestibility for the re-ensiled material. All changes were explained by the higher-effluent production and DM loss of re-ensiled material that was subjected to two compactions. Microbiology was not altered by the treatments. The use of inoculant altered ash content, but it did not influence other parameters. In contrast, re-ensiling after 36 hours of aerobic exposure caused a reduction in the nutritive value of corn silage and accentuated the DM losses.

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Morphological characteristics, dry matter production, and nutritional value of winter forage and grains under grazing and split nitrogen fertilization

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n3p1473 ◽

2017 ◽

Vol 38 (3) ◽

pp. 1483

Author(s):

Loreno Egidio Taffarel ◽

Paulo Sérgio Rabello de Oliveira ◽

Euclides Reuter de Oliveira ◽

Elaine Barbosa Muniz ◽

Eduardo Eustáquio Mesquita ◽

...

Keyword(s):

Dry Matter ◽

Morphological Characteristics ◽

N Fertilization ◽

Neutral Detergent Fiber ◽

Dry Matter Production ◽

Leaf Production ◽

Nutritional Values ◽

Dry Matter Digestibility ◽

Matter Production

Morphological characteristics, dry matter production, and nutritional values of winter forage and grains were evaluated. This study was conducted from April 24, 2012 to November 7, 2013 in the Western Paraná State University (UNIOESTE), Marechal Cândido Rondon, Brazil. Pastures under one grazing and non-grazing conditions were evaluated under 120 kg N ha-1 fertilization split into two 60 kg N ha-1 treatments. Two pastures received 40 kg N ha-1 three times. IPR 126 oat, BRS Tarumã wheat, and IPR 111 triticale were the test crops. Topdressing with 40 or 60 kg N ha-1 did not change morphological characteristics until 60 d after sowing. Pastures under non-grazing that received 120 kg N ha-1 treatments were taller than the controls, whereas those under grazing that received 80 or 120 kg N ha-1 presented with higher leaf production than did the controls. Total average dry matter (DM) production in 2012 and 2013 was, respectively, 5,275 kg ha-1 and 6,270 kg ha-1 for oat, 3,166 kg ha-1 and 7,423 kg ha-1 for wheat, and 4,552 kg ha-1 and 7,603 kg ha-1 for triticale. Split N fertilization did not cause differences in the levels of crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) in the forage. Nevertheless, increases in in vitro dry matter digestibility (IVDMD) were observed in oat and wheat receiving 60 kg N ha-1 during the first graze. IVDMD did not change in oat, wheat, and triticale forages receiving 80 or 120 kg N ha-1 during the second graze. Grazing did not affect the nutritional values of wheat and triticale grains, but reduced those of oat. Therefore, the results of the present study suggest that grazing lengthens the crop cycles, and so allow the staggered sowing of summer crops.

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Meta-analysis of corn plants, green fodder (ensilage), and silages of different types of maize hybrids used in experimental conditions in Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n1p239 ◽

2020 ◽

Vol 41 (1) ◽

pp. 237

Author(s):

João Pedro Velho ◽

Pagiel Bernardi Zardin ◽

Clóves Cabreira Jobim ◽

Ione Maria Pereira Haygert-Velho ◽

Dileta Regina Moro Alessio ◽

...

Keyword(s):

Dry Matter ◽

Meta Analysis ◽

Matter Content ◽

Neutral Detergent Fiber ◽

Experimental Conditions ◽

Maize Hybrids ◽

Hybrid Type ◽

Dry Matter Digestibility ◽

Corn Plants

The objective of this study was to conduct a meta-analysis of scientific publications from January 1994 to December 2014, to evaluate the chemical composition of corn plants, green forage, and silages of three different types of maize hybrids (simple, double, and triple) used in experimental conditions in Brazil. The sub-bases Corn Cultivation and Green Fodder were composed of 49 experiments, consisting of 481 treatments, and the sub-base Silage consisted of 24 studies composed of 163 treatments, involving 478 silos. In the Corn Cultivation sub-base, the variables of spacing, plant height, grain yield, organic matter content, and total carbohydrates of maize silage differed (P < 0.05) according to the hybrid type. The number of corn plants per hectare, number of spikes per plant, and the levels of neutral detergent fiber, acid detergent fiber, hemicellulose, and lignin did not differ (P > 0.05) according to the hybrid type. In the Green Forage sub-base (ensilage), the main difference (P < 0.05) was regarding the in vitro dry matter digestibility, whereby the triple hybrids (59.38%) had lower values than the simple (73.05%) and double (72.74%) hybrids. In the Silage sub-base, the variables of organic matter, total carbohydrates, cellulose, in vitro dry matter digestibility, and pH differed (P < 0.05) according to the hybrid type. While the variables dry matter, non-fibrous carbohydrates, neutral detergent fiber, acid detergent fiber, hemicellulose, lignin, total digestible nutrients, ethereal extract, crude protein, and ammonium nitrogen did not differ (P > 0.05) according to the hybrid type. The variability in the determination of parameters, such as dry matter yield, dry matter content, and non-fibrous carbohydrates of corn silage, and the non-determination of the starch content demonstrated that the chemical compositions of Brazilian corn silage have not yet been fully clarified. The types of maize hybrids (simple, double, and/or triple) mainly differ in terms of grain production. In many situations, the silage processes are not conducted in an ideal way, and the differences as a function of the maize plants (type of hybrid) are minimized due to losses during the ensiling process.

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PSXII-25 Characterization and evaluation of the in vitro dry matter digestibility of corn stover spent used to produce edible fungi

Journal of Animal Science ◽

10.1093/jas/skz258.823 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 415-415

Author(s):

Edgar García-Orozco ◽

Gabriela Leyva-Olvera ◽

Hermilo Leal-Lara ◽

Atmir Romero-Pérez ◽

Luis Corona ◽

...

Keyword(s):

Corn Stover ◽

Dry Matter ◽

Lentinula Edodes ◽

Animal Feed ◽

White Rot Fungi ◽

Neutral Detergent Fiber ◽

White Rot ◽

Edible Fungi ◽

Dry Matter Digestibility

Abstract Corn stover (CS) is an agricultural byproduct available in many countries for animal feed. However, due to its high lignin (L) and neutral detergent fiber (NDF) content it has a low digestibility. The production of edible fungi, using CS-based substrate, is an option to improve CS digestibility, as fungi can break down the cross-links between cell wall components. The aim of the study was to evaluate the effect of fungal treated CS from 17 species of white rot fungi: Lentinula edodes (L5, L9, LC,L15), Hericium sp (Heri) and Pleurotus djamour (Pd-Pro, Pd-UTMR) Pleurotus eryngii (Pe-PQ, Pe-MB), Pleurotus ostreatus (Po-IE202, Po-IAP, Po-Psma, Po-P14, Po-POS, Po-JP, Po-P38, Po-Sfco) on crude protein (CP), L and NDF content and in vitro dry matter digestibility (IVDMD). The composition of the substrate (C) was CS (80.55%), ground sorghum (5.98%) wheat bran (3.89%), corn gluten (4.89%), CaCO3 (3.89%) and CaSO4 (0.89%). The variables were analyzed as a completely randomized design using the MIXED procedure of SAS. Means were compared with the Tukey procedure. A significant increase (P < 0.05) of CP from 10% (Po-P38) to 69% (LC) was observed, except for Pd-UTMR, Po-IAP, Po-Psma, Po-P38 and Po-Sfco that showed similar content to the C. There was a decrease (P < 0.05) in the NDF from 10% (Heri) to 34% (L15) in relation to the C. The L content did not decrease in comparison with the C (P < 0.05). Furthermore, 13 treatments significantly increased (P < 0.05) IVDMD from 24% (Po-P14) to 42% (Pd-UTMR), except for Po-P38, Po-JP, LC and Heri in comparison with the C. In conclusion, the cultivation of white rot fungi, such as Pd-UTMR, Po-IAP, Po-Psma, LC, L9 and L15 using CS as substrate, improved CP content and increased IVDMD of CS, suggesting that which can be a good alternative for feeding ruminants.

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