scholarly journals Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1441
Author(s):  
Panagiota Tsafrakidou ◽  
Konstantina Tsigkou ◽  
Argyro Bekatorou ◽  
Maria Kanellaki ◽  
Athanasios A. Koutinas
Keyword(s):  
Response Surface Methodology ◽  
Organic Acids ◽  
Response Surface ◽  
Lignocellulosic Biomass ◽  
Immobilized Cells ◽  
Effect Of Temperature ◽  
Quadratic Effect ◽  
Linear Effect ◽  
Acidogenic Fermentation ◽  
Initial Ph

Response surface methodology was used to derive a prediction model for organic acids production by anaerobic acidogenic fermentation of cellobiose, using a mixed culture immobilized on γ-alumina. Three parameters (substrate concentration, temperature, and initial pH) were evaluated. In order to determine the limits of the parameters, preliminary experiments at 37 °C were conducted using substrates of various cellobiose concentrations and pH values. Cellobiose was used as a model sugar for subsequent experiments with lignocellulosic biomass. The culture was well adapted to cellobiose by successive subculturing at 37 °C in synthetic media (with 100:5:1 COD:N:P ratio). The experimental data of successive batch fermentations were fitted into a polynomial model for the total organic acids concentration in order to derive a predictive model that could be utilized as a tool to predict fermentation results when lignocellulosic biomass is used as a substrate. The quadratic effect of temperature was the most significant, followed by the quadratic effect of initial pH and the linear effect of cellobiose concentration. The results corroborated the validity and effectiveness of the model.

scholarly journals Effects of Roselle Extract, Potato Peel Flour, and Beef Fat on the Formation of HCA of Beef Patties Studied by Response Surface Methodology

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 27
Author(s):  
Anna Judith Pérez-Báez ◽  
Martin Valenzuela-Melendres ◽  
Juan Pedro Camou ◽  
Gustavo González-Aguilar ◽  
Manuel Viuda-Martos
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Meat Products ◽  
Heterocyclic Amines ◽  
Potato Peel ◽  
Quadratic Effect ◽  
Linear Effect ◽  
Lack Of Fit ◽  
Beef Patties ◽  
Optimal Formula

Heterocyclic amines (HCA) are compounds formed by heating meat at a high temperature. Formation of HCA in meat products should be minimized by applying different approaches. The objective of this study was to investigate the effects of roselle extract (RE, 0%–1%), potato peel flour (PP, 0%–2%), and beef fat (BF, 0%–15%) on the formation of HCA in beef patties using response surface methodology. IQx, IQ, MeIQx, MeIQ, 4,8-DiMeIQx, and PhIP were identified and quantified by HPLC. Values from 0 to 18.09 ng IQx, 0.20 to 11.66 ng IQ, 0.29 to 3.93 ng MeIQx, 0.19 to 11.51 ng MeIQ, 0.65 to 1.72 ng 4,8-DiMeIQx, and 1.87 to 14.7 ng PhIP per gram of sample were obtained. All models were significant (p < 0.05), with no lack of fit (p > 0.05) and showed a determination coefficient >0.70. Roselle extract had a negative linear effect on the formation of the total HCA, while PP and BF had a positive quadratic effect. The optimal formula that minimizes the formation of HCA included 0.80% RE, 0.85% PP, and 7.54% BF. Roselle extract and potato peel are foods that can be used as ingredients to minimize the formation of HCA in beef patties.

Electrocoagulation and nanofiltration integrated process application in purification of bilge water using response surface methodology

2016 ◽  
Vol 74 (3) ◽  
pp. 564-579 ◽  
Author(s):  
Ceyhun Akarsu ◽  
Yasin Ozay ◽  
Nadir Dizge ◽  
H. Elif Gulsen ◽  
Hasan Ates ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Removal Efficiency ◽  
Cod Removal ◽  
Positive Sign ◽  
Aluminum Electrode ◽  
Integrated Process ◽  
Bilge Water ◽  
Cod Removal Efficiency ◽  
Initial Ph

Marine pollution has been considered an increasing problem because of the increase in sea transportation day by day. Therefore, a large volume of bilge water which contains petroleum, oil and hydrocarbons in high concentrations is generated from all types of ships. In this study, treatment of bilge water by electrocoagulation/electroflotation and nanofiltration integrated process is investigated as a function of voltage, time, and initial pH with aluminum electrode as both anode and cathode. Moreover, a commercial NF270 flat-sheet membrane was also used for further purification. Box–Behnken design combined with response surface methodology was used to study the response pattern and determine the optimum conditions for maximum chemical oxygen demand (COD) removal and minimum metal ion contents of bilge water. Three independent variables, namely voltage (5–15 V), initial pH (4.5–8.0) and time (30–90 min) were transformed to coded values. The COD removal percent, UV absorbance at 254 nm, pH value (after treatment), and concentration of metal ions (Ti, As, Cu, Cr, Zn, Sr, Mo) were obtained as responses. Analysis of variance results showed that all the models were significant except for Zn (P &gt; 0.05), because the calculated F values for these models were less than the critical F value for the considered probability (P = 0.05). The obtained R2 and Radj2 values signified the correlation between the experimental data and predicted responses: except for the model of Zn concentration after treatment, the high R2 values showed the goodness of fit of the model. While the increase in the applied voltage showed negative effects, the increases in time and pH showed a positive effect on COD removal efficiency; also the most effective linear term was found as time. A positive sign of the interactive coefficients of the voltage–time and pH–time systems indicated synergistic effect on COD removal efficiency, whereas interaction between voltage and pH showed an antagonistic effect.

Optimization of the Fermentation Conditions for 1-Deoxynojirimycin Production by Streptomyces lawendulae Applying the Response Surface Methodology

2011 ◽  
Vol 7 (3) ◽  
Author(s):  
Zhao-Jun Wei ◽  
Le-Chun Zhou ◽  
Hua Chen ◽  
Gui-Hai Chen
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Starch Content ◽  
Ultra Violet ◽  
Soluble Starch ◽  
High Yield ◽  
Yield Strain ◽  
Fermentation Time ◽  
Ethyl Sulfate ◽  
Initial Ph

Moranoline (1-Deoxynojirimycin, DNJ) is a piperidine alkaloid, and shows high inhibit activities to glucoamylase and ?-glucosidase. One DNJ high-yield strain of Streptomyces lawendulae was obtained after isolated form soil and mutated with the ultra violet (UV) and ethyl sulfate (DES), which named as TB-412, and can produce DNJ with 35.925 mg/L. Response surface methodology (RSM) was applied to optimize the parameters of DNJ yield from S. lawendulae TB-412. The effects of independent variables of fermentation, including time, temperature, initial pH and the soluble starch content were investigated. The statistical analysis showed that the fermentation time, pH and the soluble starch content, and the quadratics of time, temperature, pH and the soluble starch content, as well as the interactions between fermentation time and pH, and time and the soluble starch content, showed significant effects on DNJ yield. The optimal process parameters for DNJ production within the experimental range of the variables researched was at 11d, 27 °C, pH 7.5, and 8% soluble starch content. At this condition, the DNJ yield was predicted to be 42.875 mg/L.

Optimization of photocatalytic degradation of real textile dye house wastewater by response surface methodology

2016 ◽  
Vol 74 (9) ◽  
pp. 1999-2009 ◽  
Author(s):  
Sayed Mohammad Bagher Hosseini ◽  
Narges Fallah ◽  
Sayed Javid Royaee
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Flow Rate ◽  
Advanced Oxidation Process ◽  
Influential Factor ◽  
P Value ◽  
Textile Dye ◽  
Optimum Conditions ◽  
Effective Parameters ◽  
Initial Ph

This study evaluates the advanced oxidation process for decolorization of real textile dyeing wastewater containing azo and disperse dye by TiO2 and UV radiation. Among effective parameters on the photocatalytic process, effects of three operational parameters (TiO2 concentration, initial pH and aeration flow rate) were examined with response surface methodology. The F-value (136.75) and p-value &lt;0.0001 imply that the model is significant. The ‘Pred R-Squared’ of 0.95 is in reasonable agreement with the ‘Adj R-Squared’ of 0.98, which confirms the adaptability of this model. From the quadratic models developed for degradation and subsequent analysis of variance (ANOVA) test using Design Expert software, the concentration of catalyst was found to be the most influential factor, while all the other factors were also significant. To achieve maximum dye removal, optimum conditions were found at TiO2 concentration of 3 g L−1, initial pH of 7 and aeration flow rate of 1.50 L min−1. Under the conditions stated, the percentages of dye and chemical oxygen demand removal were 98.50% and 91.50%, respectively. Furthermore, the mineralization test showed that total organic compounds removal was 91.50% during optimum conditions.

Optimization of Culture Conditions for Cold-Active Cellulase Production by Penicillium cordubense D28 Using Response Surface Methodology

2011 ◽  
Vol 183-185 ◽  
pp. 994-998
Author(s):  
Shuo Dong ◽  
Nai Yu Chi ◽  
Qing Fang Zhang
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Batch Reactors ◽  
Corn Meal ◽  
Cold Active ◽  
Initial Ph ◽  
Cost Efficient

The design of an optimum and cost-efficient medium for production of cold-active cellulase by Penicillium cordubense D28 was attempted by using response surface methodology (RSM). Based on the Plackett–Burman design, corn meal, (NH4)2SO4 and branc were selected as the most critical nutrient. Subsequently, they were investigated by the Box-Behnken design. Results showed that the maximum cold-active cellulase activity of 110.4U/mL was predicted when the concentration of corn meal, (NH4)2SO4 and branc were 21.97 g/L, 2.39 g/L and 14.99 g/L, respectively. The results were further verified by triplicate experiments. The batch reactors were operated under an optimized condition of the respective corn meal, (NH4)2SO4 and branc concentration of 22 g/L , 2.4 g/L and 15 g/L , the initial pH of 6.0 and experimental temperature of 20 ± 1°C. Without further pH adjustment, the maximum cold-active cellulase activity of 109.8 U/mL was obtained based on the optimized medium with further verified the practicability of this optimum strategy.

Degradation of Paracetamol by Fenton-Like Process in Conjunction with Sonication

2015 ◽  
Vol 737 ◽  
pp. 321-324
Author(s):  
Rong Yao Wang ◽  
Xi Kui Wang
Keyword(s):  
Response Surface Methodology ◽  
Synergistic Effect ◽  
Response Surface ◽  
Acoustic Power ◽  
Ultrasonic Cavitation ◽  
Sponge Iron ◽  
Optimum Conditions ◽  
Initial Ph

The degradation of the pharmaceutical paracetamol by using Fenton-like process in conjunction with ultrasonic cavitation was investigated. An evident synergistic effect was found in the combination of sonication and Fenton-like process. Through the application of Response Surface Methodology optimization, the optimum conditions for the degradation of paracetamol were initial pH 3.0, H2O27.0 mmol·L-1and sponge iron 4 g·L-1with acoustic power of 200 W. Under these parameters could obtain 99% degradation of 100mg·L-1paracetamol solution within 30 min treatment.

Evaluation of heterogeneous photo-Fenton oxidation of Orange II using response surface methodology

2010 ◽  
Vol 62 (6) ◽  
pp. 1320-1326 ◽  
Author(s):  
Y. H. Gong ◽  
H. Zhang ◽  
Y. L. Li ◽  
L. J. Xiang ◽  
S. Royer ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Heterogeneous Catalyst ◽  
Uv Irradiation ◽  
Heterogeneous Catalysts ◽  
Ph Value ◽  
Dye Degradation ◽  
High Catalytic Activity ◽  
Orange Ii ◽  
Initial Ph

A mesoporous SBA-15 doped iron oxide (Fe2O3/SBA-15) was synthesized by co-codensation, characterized and used as heterogeneous catalysts for the photo-Fenton decolorization of azo dye Orange II under UV irradiation. Response surface methodology (RSM) was used to investigate operating condition effects, such as hydrogen peroxide concentration, initial pH and catalyst loadings, on the decolorization rate. UV irradiation is found to enhance the activity of the catalyst in the process. RSM analysis evidenced the influence of the initial pH value and H2O2 concentration on the dye degradation rate. The coupled UV/Fe2O3/SBA-15/H2O2 process at room temperature is revealed as a promising friendly process for wastewater treatment. Indeed, the use of a heterogeneous catalyst allows an easy active phase recycling without multi-step recovering while the heterogeneous catalyst used here exhibits high catalytic activity for the reaction considered.

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