scholarly journals Technological Advancements for the Detection of Antibiotics in Food Products

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1500
Author(s):  
Sonika Dawadi ◽  
Ranjita Thapa ◽  
Bindu Modi ◽  
Sobika Bhandari ◽  
Arjun Prasad Timilsina ◽  
...  
Keyword(s):  
Food Chain ◽  
Solid Phase ◽  
Food Products ◽  
Surface Enhanced Raman Spectroscopy ◽  
Extraction Methods ◽  
Food Samples ◽  
Detection Methods ◽  
The Matrix ◽  
Immunological Assays ◽  
Working Parameters

Antibiotics, nowadays, are not only used for the treatment of human diseases but also used in animal and poultry farming to increase production. Overuse of antibiotics leads to their circulation in the food chain due to unmanaged discharge. These circulating antibiotics and their residues are a major cause of antimicrobial resistance (AMR), so comprehensive and multifaceted measures aligning with the One Health approach are crucial to curb the emergence and dissemination of antibiotic resistance through the food chain. Different chromatographic techniques and capillary electrophoresis (CE) are being widely used for the separation and detection of antibiotics and their residues from food samples. However, the matrix present in food samples interferes with the proper detection of the antibiotics, which are present in trace concentrations. This review is focused on the scientific literature published in the last decade devoted to the detection of antibiotics in food products. Various extraction methods are employed for the enrichment of antibiotics from a wide variety of food samples; however, solid-phase extraction (SPE) techniques are often used for the extraction of antibiotics from food products and biological samples. In addition, this review has scrutinized how changing instrumental composition, organization, and working parameters in the chromatography and CE can greatly impact the identification and quantification of antibiotic residues. This review also summarized recent advancements in other detection methods such as immunological assays, surface-enhanced Raman spectroscopy (SERS)-based assays, and biosensors which have emerged as rapid, sensitive, and selective tools for accurate detection and quantification of traces of antibiotics.

scholarly journals Analytical methods for the determination of common booster biocides in marine samples

2012 ◽  
Vol 10 (3) ◽  
pp. 521-533 ◽  
Author(s):  
Álvaro Sánchez-Rodríguez ◽  
Zoraida Sosa-Ferrera ◽  
José Santana-Rodríguez
Keyword(s):  
Solid Phase ◽  
Soxhlet Extraction ◽  
Chromatographic Determination ◽  
Extraction Methods ◽  
Liquid Extraction ◽  
Marine Samples ◽  
Gas And Liquid Chromatography ◽  
The Matrix ◽  
Preparation Step

AbstractBooster biocides are organic compounds that are added to antifouling copper-based paints to improve their efficacy. Due to their widespread use, they are common pollutants of marine ecosystems. Some of these compounds show acute and chronic toxic effects in non-targeted organisms at concentrations as low as ng L−1. The determination of these compounds is therefore important, and for some, which are prioritized in the EU water framework directive, a necessity. Because of their low concentrations and the matrix effect, these contaminants often require a suitable sample preparation step (extraction/pre-concentration) prior to chromatographic determination. The aim of the present article is to review extraction and chromatographic methodologies related to the determination of common booster biocides in marine samples published in the scientific literature. These methodologies include liquid-liquid extraction (LLE), solid phase extraction (SPE), solid phase microextraction (SPME), single drop microextraction (SDME), Soxhlet extraction, microwave-assisted extraction (MAE), supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) as extraction methods, and both gas and liquid chromatography as determination techniques.

scholarly journals Dispersive Solid Phase Extraction for the Analysis of Veterinary Drugs Applied to Food Samples: A Review

2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Gabriela Islas ◽  
Israel S. Ibarra ◽  
Prisciliano Hernandez ◽  
Jose M. Miranda ◽  
Alberto Cepeda
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Food Samples ◽  
Veterinary Drugs ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Solid Sorbent ◽  
Wide Range ◽  
The Matrix ◽  
Carbon Based Nanomaterials

To achieve analytical success, it is necessary to develop thorough clean-up procedures to extract analytes from the matrix. Dispersive solid phase extraction (DSPE) has been used as a pretreatment technique for the analysis of several compounds. This technique is based on the dispersion of a solid sorbent in liquid samples in the extraction isolation and clean-up of different analytes from complex matrices. DSPE has found a wide range of applications in several fields, and it is considered to be a selective, robust, and versatile technique. The applications of dispersive techniques in the analysis of veterinary drugs in different matrices involve magnetic sorbents, molecularly imprinted polymers, carbon-based nanomaterials, and the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method. Techniques based on DSPE permit minimization of additional steps such as precipitation, centrifugation, and filtration, which decreases the manipulation of the sample. In this review, we describe the main procedures used for synthesis, characterization, and application of this pretreatment technique and how it has been applied to food analysis.

scholarly journals Detecção de Resíduos de Agrotóxicos no Mel de Abelha

2014 ◽  
Vol 2 (2) ◽  
pp. 17
Author(s):  
F. M. Silva ◽  
D. C. Coelho ◽  
A. V. Machado ◽  
R. O. Costa
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Pesticide Residues ◽  
High Performance ◽  
Solid Phase ◽  
Foreign Market ◽  
Extraction Methods ◽  
Uv Detector ◽  
Residue Detection ◽  
The Matrix

<p>As abelhas A. melífera são uns dos mais importantes polinizadores de culturas, além disso, elas produzem mel, própolis, geleia real e cera. O mel, principal produto da atividade apícola, utilizado como alimento, adoçante e para fins terapêuticos (apiterapia), tem a imagem de ser natural, saudável e limpo sendo esse um produto muito valorizado no mercado externo por isso, a busca por rigorosos padrões de qualidade se torna necessária para atender a um mercado consumidor cada vez mais exigente. Dessa forma a identificação da origem floral e geográfica, a ocorrência de adulterações e as contaminações, principalmente com antibióticos e agrotóxicos no mel tem se tornado uma rota importante devido os quais podem acarretar problemas de saúde ao consumidor. A dispersão da matriz em fase sólida (MSPD) combinada às técnicas cromatográficas modernas como cromatografia a gás (GC) e cromatografia a líquido (HPLC) é uma alternativa para evitar os diversos inconvenientes encontrados nos métodos clássicos de extração. A proposta desse trabalho foi pesquisar os diferentes tipos de Análises e metodologias de detecção de resíduos de agrotóxicos no mel de abelha Apresentando diferentes metodologias para a execução de estudos para validação de metodologia em métodos analítico, utilizando as técnicas de dispersão da matriz em fase sólida, cromatografia a gás acoplada à espectrometria de massas e cromatografia líquida de alta eficiência com detector espectrofotométrico com arranjo de diodos.</p><p><strong>Pesticide Residues in Honey Pesticide Residues detection in Bee honey</strong></p><pre> </pre><p><strong> </strong>A. mellifera bees are one of the most important pollinators of crops in addition they produce honey, propolis, royal jelly and wax. Honey, the main product of beekeeping, used as food, sweetener and in therapy (apitherapy), has the image of being natural, healthy and clean making a highly valued product in the foreign market so the search for rigorous standards of quality is needed to meet a consumer market increasingly demanding. Thus the identification of floral and geographical origin, the occurrence of tampering and contamination, especially with antibiotics and pesticides in honey has become an important route because which can cause health problems to consumers. The dispersion of the solid phase matrix (PDDM) combined with modern chromatographic techniques as gas chromatography (GC) and liquid chromatography (HPLC) is an alternative to avoid the various drawbacks found in classical extraction methods. The purpose of this study was to investigate the different types of analyzes and pesticide residue detection methodologies in honey Introducing different methodologies for carrying out studies for validation of a method in analytical methods, using the matrix dispersion techniques in solid phase, gas chromatography coupled to mass spectrometry and high-performance liquid chromatography with UV detector with diode array.</p>

scholarly journals European inter-comparison studies as a tool for perfecting irradiated food detection methods

Nukleonika ◽  
2021 ◽  
Vol 66 (3) ◽  
pp. 91-97
Author(s):  
Grzegorz Piotr Guzik ◽  
Jacek Michalik
Keyword(s):  
Food Products ◽  
Food Samples ◽  
Detection Methods ◽  
Paramagnetic Resonance ◽  
Irradiated Food ◽  
Analytical Skills ◽  
Leading Role ◽  
Federal Institute ◽  
Diet Supplements ◽  
Food Detection

Abstract In this paper, we present the results of inter-comparison studies on identification of irradiated food carried out by the leading European laboratories from 1991 to 2018. In 1990s, the Federal Institute for Health Protection of Consumers and Veterinary Medicine in Germany played the leading role in the organization of the inter-laboratory tests on this subject. At the beginning of the present century, the Spanish Agency for Food Safety and Nutrition and Food National Spanish Centre took over this role. In total, 47 international tests were carried out in which nearly 500 samples of alimentary products were analysed in 37 laboratories from 14 European countries. The tests were aimed at proving the reliability of analytical methods – thermoluminescence (TL), photostimulated luminescence (PSL), and electron paramagnetic resonance (EPR) spectroscopy – for identification of specific irradiated food products and to control the analytical skills and experience of participating laboratories. The results made possible a discussion on why some irradiated food samples are more difficult for identification. In general, the tests showed that TL measurements of products such as herbs, nuts, peppers, and raisins, and EPR studies of fish and chicken bones, fresh strawberries, and dried fruits could be used as reliable control methods. The challenge that control laboratories are facing now, is related to the identification of complex food products such as diet supplements or biopharmaceuticals, in which only some additives are irradiated.

scholarly journals Twenty Years ofListeriain Brazil: Occurrence ofListeriaSpecies andListeria monocytogenesSerovars in Food Samples in Brazil between 1990 and 2012

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Deyse Christina Vallim ◽  
Cristina Barroso Hofer ◽  
Rodrigo de Castro Lisbôa ◽  
André Victor Barbosa ◽  
Leonardo Alves Rusak ◽  
...  
Keyword(s):  
Food Chain ◽  
Food Products ◽  
Food Samples ◽  
Control Measures ◽  
Processed Meat ◽  
Serotype Distribution ◽  
Geographical Locations ◽  
Different Parts ◽  
Microbiological Surveillance

Listeriaspp. isolated from different food products and collected from 12 Brazilian states were sent to the Laboratory of Bacterial Zoonoses (Oswaldo Cruz Institute, Brazil) for identification. The aims of this study were to characterize these isolates, from 1990 to 2012, by using biochemical, morphological, and serotyping tests, and to analyze the distribution ofL. monocytogenesserotypes on different food products and geographical locations. Serotyping was performed using polyclonal somatic and flagellar antisera. Of 5953 isolates, 5770 were identified asListeriaspp., from which 3429 (59.4%) wereL. innocua, 2248 (38.9%) wereL. monocytogenes, and 93 (1.6%) were otherListeriaspp.L. innocuawas predominantly isolated from 1990 to 2000, whileL. monocytogeneswas from 2001 to 2012. Regarding the serotype distribution in the foods, serotypes 1/2a and 4b were most common in processed meat and ready-to-eat products, respectively; serotypes 1/2a, 1/2b, and 4b were the most common in nonprocessed meat. The results above confirm the presence of the main serotypes ofL. monocytogenesin different parts of the food chain from three regions of the country and emphasize the importance of improving the control measures, as tolerance zero policy and microbiological surveillance in Brazil.

scholarly journals Application of Raman Spectroscopic Methods in Food Safety: A Review

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 187
Author(s):  
Marlen Petersen ◽  
Zhilong Yu ◽  
Xiaonan Lu
Keyword(s):  
Raman Spectroscopy ◽  
Food Safety ◽  
Surface Enhanced Raman Spectroscopy ◽  
Food Samples ◽  
Vital Role ◽  
Detection Methods ◽  
Spectroscopic Methods ◽  
Food Contaminants ◽  
Raman Spectroscopic ◽  
Food Detection

Food detection technologies play a vital role in ensuring food safety in the supply chains. Conventional food detection methods for biological, chemical, and physical contaminants are labor-intensive, expensive, time-consuming, and often alter the food samples. These limitations drive the need of the food industry for developing more practical food detection tools that can detect contaminants of all three classes. Raman spectroscopy can offer widespread food safety assessment in a non-destructive, ease-to-operate, sensitive, and rapid manner. Recent advances of Raman spectroscopic methods further improve the detection capabilities of food contaminants, which largely boosts its applications in food safety. In this review, we introduce the basic principles of Raman spectroscopy, surface-enhanced Raman spectroscopy (SERS), and micro-Raman spectroscopy and imaging; summarize the recent progress to detect biological, chemical, and physical hazards in foods; and discuss the limitations and future perspectives of Raman spectroscopic methods for food safety surveillance. This review is aimed to emphasize potential opportunities for applying Raman spectroscopic methods as a promising technique for food safety detection.

scholarly journals Prevalence and Characterization of Coagulase Positive Staphylococci from Food Products and Human Specimens in Egypt

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 75
Author(s):  
Eman E. Abdeen ◽  
Walid S. Mousa ◽  
Sarah Y. Abdelsalam ◽  
Hanim S. Heikal ◽  
Reyad R. Shawish ◽  
...  
Keyword(s):  
Food Chain ◽  
Meca Gene ◽  
Food Poisoning ◽  
Food Products ◽  
Public Health Importance ◽  
Human Samples ◽  
Wide Range ◽  
Mrsa Strains ◽  
Minced Meat ◽  
Coagulase Positive Staphylococci

Methicillin-resistant Staphylococcus aureus (MRSA) strains have veterinary and public health importance as they are responsible for a wide range of difficult to treat infections and food poisoning. Two hundred samples (50 samples each of minced meat, beef luncheon, Karish cheese, and human samples (pus swab from open wounds)) were cultured, and MRSA strains were identified using disk diffusion tests and mecA gene-based PCR. A total of 35% (70/200) of the examined samples were confirmed as coagulase-positive S. aureus in minced meat (46%), beef luncheon (44%), Karish cheese (44%), and human samples (22%). The MRSA strains showed resistance to amoxicillin (91.4%), penicillin (97.1%), cefoxitin (85.7%), cephradine (82.9%), tetracycline (57.2%), and erythromycin (52.8%). More than half of the tested S. aureus isolates harbored the mecA gene. The sequence analysis of the mecA gene from the minced meat, Karish cheese, and human samples revealed high genetic similarities between the S. aureus isolates from these sources. In conclusion, our findings indicate a risk for the transmission of the mecA gene of S. aureus across the food chain between humans and animal food products. Further studies should focus on finding additional epidemiological aspects of the MRSA strains in food chain.

scholarly journals Methods to extract the exopolymeric matrix from biofilms: a comparative study

1999 ◽  
Vol 39 (7) ◽  
pp. 243-250 ◽  
Author(s):  
Joana Azeredo ◽  
Valentina Lazarova ◽  
Rosário Oliveira
Keyword(s):  
Organic Carbon ◽  
Comparative Study ◽  
Mixed Culture ◽  
Pure Culture ◽  
Cell Walls ◽  
Extraction Method ◽  
Extraction Methods ◽  
Suitable Method ◽  
The Matrix ◽  
Biofilm Matrix

To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization. Glutaraldehyde is a bifunctional reagent that binds to cell walls avoiding their permeabilization and the biofilm matrix is solubilized in the solution.

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