scholarly journals The Qualitative Differences for Photosynthetic Content of Common Bean (Phaseolus vulgaris L.) Populations  in Kosovo

2014 ◽  
Vol 6 (3) ◽  
pp. 331-334
Author(s):  
Sali Ali ALIU ◽  
Imer RUSINOVCI ◽  
Shukri FETAHU ◽  
Bekim GASHI

Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossings. Twenty (20) common bean populations were analyzed using qualitative traits, chlorophyll “a” (Chl ‘a’), chlorophyll “b” (Chl ‘b’), total chlorophyll “a+b” (Total Chl) and carotenoides. The design of the experiment was conducted with leaves of common bean collected from different regions of Kosovo. The experiment was completely randomly with four repetitions. Pigments were extracted by grinding 80-100 mg freshly sampled leaves in 80% (v/v) acetone/water containing MgCO3, at room temperature, preserved in the dark for 24 hours. Concentration of chlorophyll and carotenoid content was measured by spectrophotometer using absorbance recorded at 663 nm, 644 nm and 452.3 nm for maximum absorption of Chl ‘a’, Chl ‘b’, and carotenoids respectively. According to our data the differences between populations for Chl ‘a’, and Chl ‘b’ was significantly higher at level of probability LSDp=0.01. The average values for Chl ‘a’, was 1.67 mg.g-1, while for Chl‘b’was 0.74 mg.g-1. In addition, the results for carotenoids content between populations were with high differences.

2017 ◽  
Vol 1 (4) ◽  
pp. 18
Author(s):  
S. Manikandan ◽  
P. Thamizhiniyan

The present study was conducted to characterize the effect of organic and inorganic fertilizer on the phytochemical constituents of sun flower. The phytochemical constituent such as chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content were analyzed. From the result it was observed that both the treatments had significantly improved the phytochemical constituents of sunflower. Among the treatments, organic fertilizer is found to be more efficient than inorganic fertilizer over control. The present work indicates towards the beneficial effect or organic and inorganic fertilizers for the improvement of sunflower.


2016 ◽  
Vol 4 (4) ◽  
pp. 103-107
Author(s):  
Deepa Srivastava ◽  
K. Shukla

Photosynthetic effects of the leaves extracts from Ipomoea cairica on Partheniumhysterophorus L were studied. Ipomoea cairica dried leaf powder (20g) was dissolved in distilled water (200ml) for foliar application. The seedlings were maintained under natural condition in Insect free net house. The experiment was carried out to analyze the effects of Ipomoea cairica leaves extract by measuring chlorophyll ‘a’, chlorophyll ‘b’, total chlorophyll and carotenoid contents in Parthenium. The experimental plants were sprayed 20 days after emergence and control plants were maintained by a simultaneous spray of distilled water. Leaves of Partheniumhysterophorus were collected at 5 days interval following the foliar application of Ipomoea cairica leaves extract up to 15th day. Results showed that Chlorophyll (total chlorophyll, chlorophyll a and b) and carotenoid content was adversely affected as the duration of Ipomoea cairica leaves extract increased. This potential of Ipomoea cairica can be used as herbicide to control noxious weed Partheniumhysterophorous.


2005 ◽  
Vol 57 (4) ◽  
pp. 283-290 ◽  
Author(s):  
Biljana Bojovic ◽  
J. Stojanovic

Determination of chlorophyll content as an indirect method of estimating the productivity of vegetation represents a good way to gain an understanding of the photosynthetic regime of plants. Physiological investigations of chlorophyll and carotenoid content in the uppermost internode of several wheat cultivars were carried out at the outset of the flowering phase. The dependence of chlorophyll and carotenoid content on the fertilization variant was established at that time. The tested wheat cultivars were grown under conditions of five fertilization variants. The content of chlorophyll a, chlorophyll b, and total chlorophyll (Chl a+b) was measured and carotenoid content was determined on each variant. The results indicate that chlorophyll and carotenoid content depended on the presence and ratio of mineral elements in the substrate. This is demonstrated by the variant with unfertilized soil, where chlorophyll and carotenoid content in all cultivars was lowest. The variant of fertilization with N and P turned out to be most favorable. The next most favorable variant was the one with nitrogen alone, and it was followed by the variant with N and K.


2014 ◽  
Vol 1073-1076 ◽  
pp. 118-121
Author(s):  
Qing Qing He ◽  
Zhou Li Liu ◽  
Wei Chen ◽  
Lin Hao ◽  
Jiao Jiao Qin ◽  
...  

The photosynthetic pigments of Sagittaria sagittifolia, including total chlorophyll content, chlorophyll a content, chlorophyll b content and carotenoid content, were investigated after 0, 7, 14 and 21 d exposure to 3 mg L-1 P. After 7 d P-exposure, total chlorophyll content, chlorophyll a content and chlorophyll b content increased significantly compared with the control, and then decreased with time extended. Carotenoid content increased significantly after 14 d P-exposure compared with the control, and then decreased slightly after 21 d P-exposure.


2021 ◽  
Vol 35 (2) ◽  
Author(s):  
Amine Khoulati ◽  
E. Saalaoui

An experiment was carried out in a greenhouse to study the effect of aqueous extracts of Crocus sativus L. by-products on tomato plants. Three concentrations of tepals and corms were used by fertigation: 1 g/L, 2 g/L, and 3 g/L. The aqueous extract of the stigmas was used as a foliar application at 0.6 g/L. The experiment was carried out in a completely randomized block with three repetitions for each concentration. The concentration of tepal extract at 3 g/L significantly (p≤0.05) increased the plants' height, the chlorophyll a, b content. The same results were observed for the foliar treatment with stigmas; however, there was no effect of tepal extract on the carotenoid content. On the other hand, the concentration 2 g/L of the corms extract had a positive impact (p≤0.05) in the chlorophyll b content while the concentration of 3 g/L increased the plant's height, the chlorophyll a (p≤0.05). Current results indicate that Crocus sativus by-products could improve certain physiological aspects of the recipient plants and new and natural biostimulants.


Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.


2019 ◽  
Vol 60 (12) ◽  
pp. 2672-2683 ◽  
Author(s):  
HyunSeok Lim ◽  
Ayumi Tanaka ◽  
Ryouichi Tanaka ◽  
Hisashi Ito

Abstract In plants, chlorophyll (Chl) a and b are interconvertible by the action of three enzymes—chlorophyllide a oxygenase, Chl b reductase (CBR) and 7-hydroxymethyl chlorophyll a reductase (HCAR). These reactions are collectively referred to as the Chl cycle. In plants, this cyclic pathway ubiquitously exists and plays essential roles in acclimation to different light conditions at various developmental stages. By contrast, only a limited number of cyanobacteria species produce Chl b, and these include Prochlorococcus, Prochloron, Prochlorothrix and Acaryochloris. In this study, we investigated a possible existence of the Chl cycle in Chl b synthesizing cyanobacteria by testing in vitro enzymatic activities of CBR and HCAR homologs from Prochlorothrix hollandica and Acaryochloris RCC1774. All of these proteins show respective CBR and HCAR activity in vitro, indicating that both cyanobacteria possess the potential to complete the Chl cycle. It is also found that CBR and HCAR orthologs are distributed only in the Chl b-containing cyanobacteria that habitat shallow seas or freshwater, where light conditions change dynamically, whereas they are not found in Prochlorococcus species that usually habitat environments with fixed lighting. Taken together, our results implicate a possibility that the Chl cycle functions for light acclimation in Chl b-containing cyanobacteria.


Author(s):  
V. P. Karpenko ◽  
I. I. Mostoviak ◽  
A. A. Datsenko ◽  
R. M. Prytuliak ◽  
O. I. Zabolotnyi

The article presents the results of a field experiment to study the effects of different rates of the microbial preparation Diazobacterin under different methods of using the plant growth regulator Radostim (seed treatment before sowing and spraying crops) on the content of chlorophyll a and b in buckwheat leaves of Yelena variety, their sum and carotenoids. Determination of chlorophyll a and b, their sum and carotenoids was performed in selected samples of buckwheat leaves in the field conditions, in the phases of stem branching and the beginning of flowering according to the method described by V.F. Gavrylenko and T.V. Zhyhalova using a spectrophotometer. The pigment concentration was calculated from Holm-Wettstein's equations for 100 % acetone. It was found that the content of photosynthetic pigments in buckwheat leaves is conditioned by the weather, rates and methods of application of the studied microbial preparations and phases of plant development. In particular, the analysis of chlorophyll and carotenoid content in the early flowering phase of buckwheat plants showed a significant increase compared to the phase of stem branching, which may be due to increased physiological and biochemical processes in plants, against the background of improving mineral nutrition, growth and plant development. Thus, with the multiple use of the microbial preparation Diazobacterin in the rates of 150, 175 and 200 ml and the stimulator of plant growth Radostim in the rate of 250 ml / t for seed treatment before sowing, the content of chlorophylls a and b, their sum and carotenoids with increasing application of Diazobacterin increased and the excess relative to control ranged within: 29–31 % for chlorophyll a; 10–11 % for chlorophyll b; 24–26 % for their sum and 23–24 % for carotenoids. At the same time, with a complex application for pre-sowing seed treatment of a mixture of the microbial preparation Diazobacterin in the rate of 200 ml per hectare of seeds with the stimulator of plant growth Radostym in the rate of 250 ml / t followed by spraying on this background crops the stimulator of plant growth Radostim in the rate of 50 ml / ha buckwheat leaves form the highest content of chlorophyll a in comparison with the control (40 %); chlorophyll b (15 %); the sum of chlorophyll a + b (33 %) and carotenoids (29 %). These data indicate the creation of more favorable conditions for physiological and biochemical processes in plants, including photosynthesis, with the direct positive effect of which, functionally active pigment complex of the leaf apparatus of buckwheat is formed.


2020 ◽  
pp. 1876-1882
Author(s):  
Evander Alves Ferreira ◽  
Marcia Vitória Santos ◽  
Leandro Diego da Silva ◽  
Priscila Júnia Rodrigues da Cruz ◽  
Raul Ribeiro Silveira ◽  
...  

The aim of this work was to study the effect of sub-doses of herbicide nicosulfuron on the levels of chlorophyll and growth of marandu-grass in an forest-livestock integration system. The treatments consisted of eucalyptus consortium with sorghum (BRS 655 hybrid) and forage Urochloa brizantha cv. Marandu (marandu-grass) at 12×2 and 12×3 m spacings in agroforestry systems, in addition to sorghum + marandu-grass and marandu-grass in monoculture. The doses of nicosulfuron applied at the marandu-grass were 0, 15 and 30 g ha-1. At 30 and 120 days after the application of nicosulfuron, chlorophyll a and chlorophyll b were measured usinf a portable chlorophyll meter, with six measurements per plant. Afterwards, the total chlorophyll content and chlorophyll a/b were calculated. We also measured the mass of the total dry matter of marandu-grass and growth rate of the culture. Marandu-grass plants cultivated in monoculture showed higher quantities of chlorophyll and the highest growth rates. The levels of chlorophyll a, b and chlorophyll a/b and the growth rate of marandu grass were similar between systems integrated with eucalyptus (12x2 and 12x3 m). The increase of herbicide dose negatively influenced on chlorophyll content in marandu-grass in all treatments evaluated. The use of nicosulfuron in doses of 25 and 50 g L-1 reduced the growth rate of marandu grass only when grown in monoculture


2018 ◽  
Vol 9 (2) ◽  
pp. 282-286
Author(s):  
Daniel Baron ◽  
Gustavo Francisco Rosalin Saraiva ◽  
Talita Silveira Amador ◽  
João Domingos Rodrigues ◽  
Rumy Goto ◽  
...  

The grafting of two cucumber scions (Cucumis sativus L.)—Taiko hybrid and Tsuyoi hybrid—onto a Tropical hybrid pumpkin rootstock (Cucurbita moschata L.) was studied. The study was conducted in a greenhouse. Plants grafted using the tongue approach grafting technique were compared with ungrafted plants. We evaluated the initial establishment of grafting through anatomical cuts on the region of union graft and examining leaves’ chlorophyll index (SPAD) and their levels of chlorophyll a, chlorophyll b and total chlorophyll. The experimental design utilized randomized blocks and the data were subjected to variance analysis (ANOVA) and the Tukey Test at 5% probability. The results indicate that Taiko cucumber scions are best grafted onto Tropical pumpkin rootstock, since these exhibits early grafting establishment. Moreover, when compared to grafting using Tsuyoi cucumber scions, Taiko cucumber scions present higher SPAD index values and levels of chlorophyll a and total chlorophyll. 


Sign in / Sign up

Export Citation Format

Share Document