scholarly journals Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2702
Author(s):  
Madalina M. Barsan ◽  
Caroline G. Sanz ◽  
Melania Onea ◽  
Victor C. Diculescu
Keyword(s):  
Morphological Analysis ◽  
Morphological Changes ◽  
20S Proteasome ◽  
Gravimetric Analysis ◽  
Serum Samples ◽  
Self Assembled Monolayer ◽  
Immobilized Antibodies ◽  
Oriented Immobilization ◽  
Dual Strategy ◽  
Detection Strategies

A dual strategy for the electrochemical detection for 20S proteasome (20S) is proposed, based on the oriented immobilization of a capture monoclonal antibody (Abβ) on a self-assembled monolayer of 4-mercaptophenylboronic acid (4-MPBA) on gold electrodes, which led to the Au/4-MPBA/Abβ immunosensor. The methodology comprises the correlation of 20S concentration with (i) its proteolytic activity toward the Z-LLE-AMC substrate, using the Au/4-MPBA/Abβ/20S, and (ii) the enzymatic activity of an alkaline phosphatase (AlkP) from the AlkP-labeled secondary antibody (Abcore-AlkP), which involves the conversion of aminophenylphosphate to the electroactive aminophenol using Au/4-MPBA/Abβ/20S/Abcore-AlkP. The step-by-step construction of the immunosensor and the interactions at its surface were evaluated by surface plasmon resonance and gravimetric analysis with quartz crystal microbalance, showing a high affinity between both antibodies and 20S. Morphological analysis by scanning electron microscopy demonstrated a pattern of parallel lines upon immobilization of Abβ on 4-MPBA and morphological changes to a well-organized granular structure upon binding of 20S. A voltametric and impedimetric characterization was performed after each step in the immunosensor construction. The two detection strategies were evaluated. It was shown that the immunosensor responds linearly with 20S concentration in the range between 5 and 100 µg mL−1, which corresponds to proteasome levels in serum in the case of diverse pathological situations, and LoD values of 1.4 and 0.2 µg mL−1 were calculated for the detection strategies. The immunosensor was applied to the detection of 20S in serum samples with recovery values ranging from 101 to 103%.

scholarly journals Effects of thermal treatments on characteristics and morphological variations in the deposits of urea-SCR systems

2021 ◽  
Author(s):  
Sadashiva Prabhu S ◽  
Kapilan Natesan ◽  
Nagaraj Shivappa Nayak
Keyword(s):  
Cyanuric Acid ◽  
Catalytic Reduction ◽  
Morphological Changes ◽  
Water Solution ◽  
Slight Variation ◽  
Gravimetric Analysis ◽  
Oxides Of Nitrogen ◽  
Deposit Formation ◽  
X Ray ◽  
Exhaust Gases

AbstractSelective catalytic reduction (SCR) systems are employed by automobile manufacturers for the abatement of environmental pollutants like oxides of nitrogen (NOx) emitted from exhaust gases of diesel engines. In SCR, the urea-water solution (UWS) is injected to exhaust gases in the form of a spray to generate the reducing agent NH3. Deposit formation at lower temperatures is a major concern with this technology. The deposits not only create backpressure but also leak NH3 to the environment as they deplete. It is very important to know the depletion characteristics of deposits formed at lower temperatures in order to assess the NH3 leakage to the environment when the engine exhaust gases attain higher temperatures. In the present work, deposits formed at a low-temperature range of 150–200°C for continuous run along with UWS injection were investigated. Additionally, they were aged at 300°C in the absence of UWS to check the variation in characteristics with the rise of temperature. By gravimetric analysis, it is inferred that the deposits formed at higher pre-age temperatures are less prone to depletion as the temperature increases. The elemental analysis using energy-dispersive X-ray spectroscopy (EDX) indicates slight variation in carbon, nitrogen and oxygen compositions for all the pre-age conditions. As an extended study, the byproducts at pre-age and post-age conditions were investigated through X-ray diffraction (XRD). The compounds like cyanuric acid (CYA) and biuret were not observed when pre-age samples were aged at 300°C. Instead, the compounds like ammelide, ammeline, triuret and melamine were observed. Scanning electron microscope (SEM) study revealed morphological changes in both pre-age and post-age samples. Further, the crystallinity variations were also observed for the changes in the heating cycles during deposit formation. The gravimetric analysis of deposits in pre-age and post-age conditions helps in predicting the amount of deposits for transient load cycles.

scholarly journals Action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after nerve injury

2017 ◽  
Vol 15 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Ana Luiza Peretti ◽  
Juliana Sobral Antunes ◽  
Keli Lovison ◽  
Regina Inês Kunz ◽  
Lidyane Regina Gomes Castor ◽  
...  
Keyword(s):  
Nerve Injury ◽  
Tibialis Anterior ◽  
Morphological Analysis ◽  
Morphological Changes ◽  
Control Group ◽  
Vanilla Planifolia ◽  
Morphological Alterations ◽  
Injury Group ◽  
Fiber Atrophy ◽  
Oral Doses

ABSTRACT Objective To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle.

scholarly journals A novel protein biochip screening serum anti-sperm antibody expression and natural pregnancy rate in a follow-up study in Chinese infertility

2020 ◽  
Vol 40 (2) ◽  
Author(s):  
Feihong Xu ◽  
Lei Ye ◽  
Yuan Hu ◽  
Chengyun Cai ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Serum Samples ◽  
Self Assembled Monolayer ◽  
Autoimmune Reaction ◽  
Protein Assay ◽  
Follow Up Study ◽  
Healthy Donors ◽  
Positive Rate ◽  
Reproductive Processes

Abstract Production of anti-sperm antibody (ASA) often suffers from autoimmune reaction against sperms in human infertility. The antibodies are measured in both blood and seminal plasma of males. Here, we reported a simple protein biochip methodology that takes advantage of a functionalized self-assembled monolayer modified by N-hydroxysuccinimide (NHS) and enables identification of anti-sperm antibody in Chinese male infertility. To validate this biochip platform, we immobilized purified sperm protein on the biochip surface and tested a variety of parameters in quality controls for the protein assay, respectively. Then, we analyzed serum samples from 368 patients with infertility and 116 healthy donors by means of this biochip simultaneously. We found that positive rate of serum ASA was 20.92% (77/368) in the cases and 1.72% (2/116) in the controls, respectively. Furthermore, we further corroborated the biochip assay in comparison with ELISA method. We found that both methods were compatible for the detection of serum ASA in the patients. In addition, a follow-up study for natural conception in ASA-positive and ASA-negative patients was conducted. The result showed a significant correlation between serum ASA expression and natural pregnancy rate 6.5% in ASA-positive patients while 18.9% in ASA-negative patients, indicating the potential roles of ASA in naturally reproductive processes.

Oriented immobilization of antibodies by a self-assembled monolayer of 2-(biotinamido)ethanethiol for immunoarray preparation

2006 ◽  
Vol 47 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Se-Hui Jung ◽  
Ha-Young Son ◽  
Jong Seol Yuk ◽  
Jae-Wan Jung ◽  
Kyung Hwan Kim ◽  
...  
Keyword(s):  
Self Assembled Monolayer ◽  
Oriented Immobilization ◽  
Self Assembled

The verbs ‘rain’ and ‘snow’ in Gallo-Romance, and other morphological mismatches in diachrony

2020 ◽  
pp. 385-402
Author(s):  
Martin Maiden
Keyword(s):  
Semantic Similarity ◽  
Morphological Analysis ◽  
Morphological Changes ◽  
Prima Facie ◽  
Lexical Meaning ◽  
Lexical Semantic ◽  
Word Forms ◽  
Entire Word

The historical morphology of the verb ‘snow’ in Francoprovençal presents a conundrum, in that it is clearly analogically influenced by the verb ‘rain’, for obvious reasons of lexical semantic similarity, but the locus of that influence is not the ‘root’ (the ostensible bearer of lexical meaning) but desinential inflexion-class members, which are in principle independent of any lexical meaning. Similar morphological changes are also identified for other Gallo-Romance verbs. It seems, in effect, that speakers can identify exponents of the lexical meaning of word-forms in linear sequences larger than the apparent ‘morphemic’ composition of those word-forms, even when such a composition may seem prima facie transparent and obvious. It is argued that these facts are inherently incompatible with ‘constructivist’, morpheme-based, models of morphology, and strongly compatible with what have been called ‘abstractivist’ (‘word-and-paradigm’) approaches, which generally take entire word-forms as the primary units of morphological analysis.

Magnetic Resonance Imaging in Non-Traumatic Canalicular Peripheral Neuropathy of the Arm

1998 ◽  
Vol 11 (1) ◽  
pp. 39-42
Author(s):  
F.A. Lupo ◽  
A. Paladini
Keyword(s):  
Morphological Analysis ◽  
Morphological Changes ◽  
Clinical Syndrome ◽  
Nerve Lesion ◽  
Resonance Imaging ◽  
Anatomical Changes ◽  
Appropriate Treatment ◽  
Electrophysiological Findings ◽  
The Cost

The term canalicular neuropathy is applied to nerve lesions arising on nerve segments passing through the osteofibrous canals or other narrow orifices and resulting in nerve compression and entrapment. We studied 31 patients referred to us for non-traumatic canalicular syndromes in the upper limbs. After clinical, electrophysiological and morphological analysis of each case, we emphasize the role of MR imaging in establishing prognosis and selecting treatment. Anatomosurgical specimens correlated well with MR features, whereas there was little correlation between electrophysiological severity and anatomical changes. In summary, canalicular neuropathy is a clinical syndrome. The aim of MR investigation is to document the morphological changes which will serve to institute appropriate treatment. When patients have clinical and electrophysiological evidence of neuropathy without MR demonstration of the nerve lesion, therapy will be conservative, thus saving the cost and risk of surgery. On the other hand, when clinical and electrophysiological findings are flanked by MR demonstration of the neuropathy, the disease is known to be advanced and surgery will be indicated to prevent neurotmesis.

scholarly journals Recovery of cutting oil from wastewater by pervaporation process using natural clay modified PVA membrane

2019 ◽  
Vol 80 (12) ◽  
pp. 2404-2411
Author(s):  
D. Unlu
Keyword(s):  
Morphological Analysis ◽  
Poly Vinyl Alcohol ◽  
Gravimetric Analysis ◽  
Thermal Gravimetric ◽  
Natural Clay ◽  
Feed Mixture ◽  
Feed Concentration ◽  
Feed Temperature ◽  
Thermal Stability Of ◽  
Clay Kaolin

Abstract In this study, the pervaporative dehydration of the cutting oil ‘diethylene glycol’ (DEG) through a hydrophilic PVA membrane was investigated at various operation temperatures in the range of 333–363 K with a feed mixture containing 0.5–2.0 wt% water. The pervaporation (PV) performance of poly(vinyl alcohol) (PVA) is enhanced by the addition of natural clay kaolin into the pristine membrane. The thermal stability of the membranes was analyzed by thermal gravimetric analysis (TGA). The morphological analysis of the membranes was performed by scanning electron microscope (SEM). Separation success was determined by calculation of flux, selectivity, and PSI. These values were investigated as functions of the clay amount, feed concentration and feed temperature. The obtained results show that PV is an effective method for recycling waste cutting oil from wastewater.

scholarly journals Morphological analysis of cerium oxide stabilized nanoporous gold catalysts by soft X-ray ASAXS

RSC Advances ◽  
2017 ◽  
Vol 7 (72) ◽  
pp. 45344-45350 ◽  
Author(s):  
C. Rumancev ◽  
A. R. von Gundlach ◽  
S. Baier ◽  
A. Wittstock ◽  
J. Shi ◽  
...  
Keyword(s):  
Cerium Oxide ◽  
Morphological Analysis ◽  
Morphological Changes ◽  
Gold Catalyst ◽  
Gold Catalysts ◽  
Nanoporous Gold ◽  
Morphological Properties ◽  
X Ray ◽  
Stabilizing Effect

Soft X-ray SAXS and ASAXS reveal nanostructural properties and temperature induced morphological changes in catalyst materials. The stabilizing effect of cerium oxide deposits on the gold catalyst and the morphological properties of the cerium oxide were determined.

scholarly journals THE COMPARATIVE MORPHOLOGICAL ANALYSIS OF THE NEPHROTOXICITY OF DOXORUBICIN AND NANOSTRUCTURED DOXORUBICIN IN CONDITIONS OF TRANSPLANTABLE CARCINOGENESIS

2018 ◽  
Vol 26 (2) ◽  
pp. 45-48
Author(s):  
I. N. Chairkin ◽  
N. V. Chairkina ◽  
O. N. Deryabina ◽  
V. Y. Medvezhonkov ◽  
O. V. Kalmin
Keyword(s):  
Morphological Analysis ◽  
Structural Changes ◽  
Morphological Changes ◽  
Control Group ◽  
Structural Components ◽  
Laboratory Rats ◽  
Chemotherapy Drugs ◽  
Morphological Evaluation ◽  
Comparative Morphological Analysis ◽  
Convoluted Tubules

The study was carried out on 120 mongrel white laboratory rats, to whom were transplanted the ascitiс Zaidel's hepatoma and which were divided into three equal groups. Of these, the first group of animals was a control group without treatment. In the second group, to animals doxorubicin was injected, in the third group, nanostructured doxorubicin was injected to the animals for 21 days. The aim of the study was to assess the degree of morphological changes in the tubules and glomeruli of the kidneys under the influence of nanostructured doxorubicin and doxorubicin in the condition of transplantable carcinogenesis. Given the nephrotoxicity of the chemotherapy drugs studied, the dynamics of structural changes in the tubules and glomeruli of the kidneys was studied. Conducted a comparative morphological evaluation of the changes occurring in the kidneys. The results of a morphological study of the structural components of the kidneys prove that doxorubicin and nanostructured doxorubicin have different effects on both the tubular apparatus and the glomeruli of the kidneys in the condition of transient carcinogenesis. The use of doxorubicin is accompanied by a relatively pronounced nephrotoxic effect, which is indicated by dystrophic and necrobiotic changes in the epithelium of convoluted tubules and the glomerular apparatus of the kidneys. The use of nanostructured doxorubicin is limited to moderately expressed dystrophic changes in the epithelium of the tubular apparatus of the kidneys, the glomerular apparatus remains intact at the same time. Thus, the use of the preparation of nanostructured doxorubicin for the treatment of the ascitic hepatoma of Zaidel in experimental animals has a less pronounced toxic and damaging effect on the renal parenchyma.

scholarly journals Characterization for the necessity of thermophilic biogas digester of tea waste and cooked waste for biogas production

2020 ◽  
Vol 9 (3) ◽  
pp. 159
Author(s):  
Nirmal Halder
Keyword(s):  
Morphological Analysis ◽  
Biogas Production ◽  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Thermo Gravimetric ◽  
Tea Waste ◽  
Thermophilic Digestion ◽  
First Time ◽  
Novel Design

<p><span>Characterization of tea waste, cooked waste has been done by various authors but for the first time it has been done for understanding the necessity of thermophilic digestion. And for this kind of digestion takes place in thermophilic digester for efficient biogas production. Detailed morphological analysis of feedstock has been determined. In the present study, thermo gravimetric analysis carried out For easy and fast digestion of cooked waste, a novel design of thermophilic digester is proposed and tested.</span></p>

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