scholarly journals Use of Thermally Assisted Hydrolysis and Methylation (THM-GC-MS) to Unravel Influence of Pottery Production and Post-Depositional Processes on the Molecular Composition of Organic Matter in Sherds from a Complex Coastal Settlement

Separations ◽  
2021 ◽  
Vol 8 (9) ◽  
pp. 140
Author(s):  
Joeri Kaal ◽  
María Guadalupe Castro González ◽  
Antonio Martínez Cortizas ◽  
María Pilar Prieto Martínez
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Bronze Age ◽  
Molecular Composition ◽  
Color Analysis ◽  
Pottery Production ◽  
Depositional Processes ◽  
Prehistoric Pottery ◽  
Wide Range ◽  
Coastal Deposits

Ceramic fragments from the Islet of Guidoiro Areoso (NW Spain), covering a wide range of cultural periods (Neolithic to Late Bronze Age), have been studied by color analysis, elemental analysis of carbon (C) and nitrogen (N), and molecular analysis (thermally assisted hydrolysis and methylation, THM-GC-MS), in order to identify the organic matter (OM) in the prehistoric pottery and reveal information on ceramic production techniques, food remains and post-depositional effects. Results showed that the strong marine influence (sherds recovered from coastal deposits) and microbial activity (recovery from waste deposits, “cuncheiros”) had a profound effect on C/N ratio and molecular composition (N-rich protein and chitin structures). Other organic ingredients originated from the material used for creating the ware (detected as pyrogenic OM) and possibly food remains (fatty acid fingerprints). Dark-colored ware was enriched in both pyrogenic OM from incomplete combustion and non-bacterial fatty acids. Fatty acid patterns could not be related to possible vessel use, and markers of aquatic resources were scarce, or absent. It is argued that THM-GC-MS of pottery fragments is useful for understanding how an archaeological deposit developed in time, what kinds of OM are present, and possibly to make a pre-selection of samples with high potential for more cost-demanding dietary molecular assessments.

scholarly journals Local provenance of raw materials for prehistoric pottery production at the Spasovine tin placer site (Western Serbia)

Starinar ◽  
2020 ◽  
pp. 41-50
Author(s):  
Wayne Powell ◽  
Lina Pacifico ◽  
Terrence Mitchell ◽  
Steffanie Cruse ◽  
Arthur Bankoff
Keyword(s):  
Bronze Age ◽  
Raw Materials ◽  
Late Bronze Age ◽  
Pottery Production ◽  
Group Mineral ◽  
Prehistoric Pottery ◽  
Pottery Sherds ◽  
The Town ◽  
Local Materials ◽  
Ore Grade

Archaeological finds at Spasovine, on the south flank of Mt Cer, near the town of Milina, indicate that it was settled in the Eneolithic and seasonally inhabited for tin placer mining in the Late Bronze Age. The site is highly disturbed and abraded domestic pottery is the most common material found. An analysis of the mineralogical assemblages that comprise the temper sand in a subset of the prehistoric pottery sherds from the site indicate that the sand was obtained from the adjacent Milinska River. Key minerals that link the pottery to on-site production from local materials include almandine-spessartine series garnets, the tin-bearing mineral cassiterite (SnO2) and a microlite group mineral ([Ca,Sn,U]2[Ta,Nb]2O6(OH,F]). The unusually common occurrence of cassiterite within the pottery sherds relative to the abundance in the Milinska today suggests that the tin ore grade in the Milinska River may have been significantly higher in prehistory.

Coagulation/flocculation/ultrafiltration for natural organic matter removal in drinking water production

2004 ◽  
Vol 4 (5-6) ◽  
pp. 215-222 ◽  
Author(s):  
A.R. Costa ◽  
M.N. de Pinho
Keyword(s):  
Drinking Water ◽  
Organic Matter ◽  
Natural Organic Matter ◽  
Membrane Fouling ◽  
Membrane Filtration ◽  
Membrane Surface ◽  
Water Production ◽  
Cellulose Acetate Membranes ◽  
Wide Range ◽  
Drinking Water Production

Membrane fouling by natural organic matter (NOM), namely by humic substances (HS), is a major problem in water treatment for drinking water production using membrane processes. Membrane fouling is dependent on membrane morphology like pore size and on water characteristics namely NOM nature. This work addresses the evaluation of the efficiency of ultrafiltration (UF) and Coagulation/Flocculation/UF performance in terms of permeation fluxes and HS removal, of the water from Tagus River (Valada). The operation of coagulation with chitosan was evaluated as a pretreatment for minimization of membrane fouling. UF experiments were carried out in flat cells of 13.2×10−4 m2 of membrane surface area and at transmembrane pressures from 1 to 4 bar. Five cellulose acetate membranes were laboratory made to cover a wide range of molecular weight cut-off (MWCO): 2,300, 11,000, 28,000, 60,000 and 75,000 Da. Severe fouling is observed for the membranes with the highest cut-off. In the permeation experiments of raw water, coagulation prior to membrane filtration led to a significant improvement of the permeation performance of the membranes with the highest MWCO due to the particles and colloidal matter removal.

scholarly journals Integrating Geomorphological Data, Geochronology and Archaeological Evidence for Coastal Landscape Reconstruction, the Case of Ammoudara Beach, Crete

Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1269
Author(s):  
George Alexandrakis ◽  
Stelios Petrakis ◽  
Nikolaos A. Kampanis
Keyword(s):  
Bronze Age ◽  
Coastal Area ◽  
Luminescence Dating ◽  
Coastal Sediments ◽  
Aerial Photographs ◽  
Coastal Landscape ◽  
Archaeological Evidence ◽  
Dune Field ◽  
Coastal Landscapes ◽  
Coastal Deposits

Understanding the processes that govern the transformation of the landscape through time is essential for exploring the evolution of a coastal area. Coastal landscapes are dynamic sites, with their evolution strongly linked with waves and sea level variations. Geomorphological features in the coastal area, such as beachrock formations and dune fields, can function as indicators of the coastal landscape evolution through time. However, our knowledge of the chronological framework of coastal deposits in the Aegean coasts is limited. Optically Stimulated Luminescence dating techniques are deemed to be very promising in direct dating of the coastal sediments, especially when they are linked with archaeological evidence. The dating of the sediments from different sediment core depths, determined by the method of luminosity, allowed us to calculate the rate of sediment deposition over time. More recent coastal evolution and stability were examined from 1945 to 2020 with the use of aerial photographs and satellite images. This paper presents the 6000 ka evolution of a coastal landscape based on geomorphological, archaeological, and radio-chronological data. Based on the results, the early stages of the Ammoudara beach dune field appears to have been formed ~9.0–9.6 ka BP, while the OSL ages from 6 m depth represented the timing of its stabilization (OSL ages ~5–6 ka). This indicates that the dune field appears to already have been formed long before the Bronze Age (5–10 ka BP). It became stabilized with only localized episodes of dune reactivation occurring. In contrast, while high coastal erosion rates were calculated for modern times.

scholarly journals Engineering the Monomer Composition of Polyhydroxyalkanoates Synthesized in Saccharomyces cerevisiae

2006 ◽  
Vol 72 (1) ◽  
pp. 536-543 ◽  
Author(s):  
Bo Zhang ◽  
Ross Carlson ◽  
Friedrich Srienc
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acid ◽  
Chain Length ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Pha Synthase ◽  
Fatty Acid Degradation ◽  
Medium Chain ◽  
Acid Degradation ◽  
Wide Range

ABSTRACT Polyhydroxyalkanoates (PHAs) have received considerable interest as renewable-resource-based, biodegradable, and biocompatible plastics with a wide range of potential applications. We have engineered the synthesis of PHA polymers composed of monomers ranging from 4 to 14 carbon atoms in either the cytosol or the peroxisome of Saccharomyces cerevisiae by harnessing intermediates of fatty acid metabolism. Cytosolic PHA production was supported by establishing in the cytosol critical β-oxidation chemistries which are found natively in peroxisomes. This platform was utilized to supply medium-chain (C6 to C14) PHA precursors from both fatty acid degradation and synthesis to a cytosolically expressed medium-chain-length (mcl) polymerase from Pseudomonas oleovorans. Synthesis of short-chain-length PHAs (scl-PHAs) was established in the peroxisome of a wild-type yeast strain by targeting the Ralstonia eutropha scl polymerase to the peroxisome. This strain, harboring a peroxisomally targeted scl-PHA synthase, accumulated PHA up to approximately 7% of its cell dry weight. These results indicate (i) that S. cerevisiae expressing a cytosolic mcl-PHA polymerase or a peroxisomal scl-PHA synthase can use the 3-hydroxyacyl coenzyme A intermediates from fatty acid metabolism to synthesize PHAs and (ii) that fatty acid degradation is also possible in the cytosol as β-oxidation might not be confined only to the peroxisomes. Polymers of even-numbered, odd-numbered, or a combination of even- and odd-numbered monomers can be controlled by feeding the appropriate substrates. This ability should permit the rational design and synthesis of polymers with desired material properties.

Methyl mercury in zooplankton—the role of size, habitat, and food quality

2002 ◽  
Vol 59 (10) ◽  
pp. 1606-1615 ◽  
Author(s):  
Martin Kainz ◽  
Marc Lucotte ◽  
Christopher C Parrish
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Body Size ◽  
Methyl Mercury ◽  
Size Fraction ◽  
Food Sources ◽  
Calanoid Copepods ◽  
Bacterial Food

Pathways of methyl mercury (MeHg) accumulation in zooplankton include ingestion of organic matter (OM). We analyzed fatty acid (FA) biomarkers in zooplankton to (i) investigate the effect of allochthonous and autochthonous OM ingestion on MeHg concentrations ([MeHg]) in zooplankton and (ii) examine how algal and bacterial food sources affect MeHg bioaccumulation. We partitioned bulk zooplankton samples (i.e., >500, 202, 100, and 53 μm) from Lake Lusignan (Québec) and measured [MeHg] and [FA] in each fraction. [MeHg] increased with increasing body size and was significantly higher in pelagic than in littoral macrozooplankton (>500 μm). The amount of the ingested terrestrial FA biomarker 24:0 indicated that less than 1% of the total FA in zooplankton was derived from allochthonous sources. More than 60% of the ingested FA originated from algal biomarkers and <10% from bacterial biomarkers. Relative amounts of algal-derived essential FA and bacterial FA were not associated with [MeHg] in any size fraction. In pelagic zones, the amount of MeHg in zooplankton related positively to the number of large organisms such as Calanoid copepods and Daphnia. We propose that the accumulation of MeHg in lacustrine zooplankton depends on the zooplankton habitat rather than on the quality of ingested food.

A generally applicable pedotransfer function that estimates field capacity and permanent wilting point from soil texture and bulk density

2008 ◽  
Vol 88 (5) ◽  
pp. 761-774 ◽  
Author(s):  
J. A. P. Pollacco
Keyword(s):  
Organic Matter ◽  
Soil Moisture ◽  
Bulk Density ◽  
Soil Texture ◽  
The United States ◽  
Pedotransfer Functions ◽  
Pedotransfer Function ◽  
Data Set ◽  
Wide Range ◽  
Fitting Parameters

Hydrological models require the determination of fitting parameters that are tedious and time consuming to acquire. A rapid alternative method of estimating the fitting parameters is to use pedotransfer functions. This paper proposes a reliable method to estimate soil moisture at -33 and -1500 kPa from soil texture and bulk density. This method reduces the saturated moisture content by multiplying it with two non-linear functions depending on sand and clay contents. The novel pedotransfer function has no restrictions on the range of the texture predictors and gives reasonable predictions for soils with bulk density that varies from 0.25 to 2.16 g cm-3. These pedotransfer functions require only five parameters for each pressure head. It is generally accepted that the introduction of organic matter as a predictor improves the outcomes; however it was found by using a porosity based pedotransfer model, using organic matter as a predictor only modestly improves the accuracy. The model was developed employing 18 559 samples from the IGBP-DIS soil data set for pedotransfer function development (Data and Information System of the International Geosphere Biosphere Programme) database that embodies all major soils across the United States of America. The function is reliable and performs well for a wide range of soils occurring in very dry to very wet climates. Climatical grouping of the IGBP-DIS soils was proposed (aquic, tropical, cryic, aridic), but the results show that only tropical soils require specific grouping. Among many other different non-climatical soil groups tested, only humic and vitric soils were found to require specific grouping. The reliability of the pedotransfer function was further demonstrated with an independent database from Northern Italy having heterogeneous soils, and was found to be comparable or better than the accuracy of other pedotransfer functions found in the literature. Key words: Pedotransfer functions, soil moisture, soil texture, bulk density, organic matter, grouping

scholarly journals Molecular characterization of an end-residue of humeomics applied to a soil humic acid

RSC Advances ◽  
2014 ◽  
Vol 4 (45) ◽  
pp. 23658-23665 ◽  
Author(s):  
A. Nebbioso ◽  
A. Piccolo ◽  
M. Lamshöft ◽  
M. Spiteller
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Molecular Characterization ◽  
Natural Organic Matter ◽  
Molecular Composition ◽  
Chemical Fractionation ◽  
Soil Humic Acid

Humeomics encompasses step-wise chemical fractionation and instrumental determination to fully characterize the heterogeneous molecular composition of natural organic matter.

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