scholarly journals Production of Specific Polyclonal Antibody against the Larvae of Corallivorous Gastropod Drupella fragum for Prediction of Outbreaks

2021 ◽  
Vol 13 (21) ◽  
pp. 11713
Author(s):  
Takaya Kitamura ◽  
Toshiharu Iwai ◽  
Yui Shigematsu ◽  
Chiemi Miura ◽  
Takeshi Miura
Keyword(s):  
Field Study ◽  
Polyclonal Antibody ◽  
The Other ◽  
Sampling Point ◽  
Antibody Specificity ◽  
Large Numbers ◽  
Coral Communities ◽  
Large Groups ◽  
Specific Polyclonal Antibody

The term corallivorous gastropod refers to a group of snails that feeds on coral and inhabits coral communities worldwide. Outbreaks of these species cause serious damage to coral communities. There are various reasons behind the outbreaks; however, further clarifications are needed. It may be possible to predict outbreaks by measuring the number of floating larvae of corallivorous gastropods in seawater. Drupella fragum is the most damaging species in Japan, so we produced antibodies against D. fragum larvae in order to easily detect this species in the field. Antibody specificity analysis in aquarium-hatched corallivorous gastropods showed a higher specificity against D. fragum compared to D. cornus. A field study using the antibody showed that many D. fragum larvae were detected from June to November at all stations. The larvae at the Shirigai station were collected in June and July in large numbers compared to the other stations. Large groups of D. fragum were collected around the sampling point in Shirigai in September 2016. Our results imply that there is a possibility that outbreaks could be predicted using this antibody.

scholarly journals Risk of pre-term births and major birth defects resulting from paternal intake of COVID-19 medications prior to conception

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Silvia Rizzi ◽  
Maarten J. Wensink ◽  
Rune Lindahl-Jacobsen ◽  
Lu Tian ◽  
Ying Lu ◽  
...  
Keyword(s):  
Cohort Study ◽  
Birth Outcomes ◽  
Birth Defects ◽  
Sperm Quality ◽  
The Other ◽  
Reproductive Age ◽  
Adverse Birth Outcomes ◽  
Term Birth ◽  
Large Numbers ◽  
Term Births

Abstract Objective With the ongoing COVID-19 pandemic, large numbers of people will receive one of the several medications proposed to treat COVID-19, including patients of reproductive age. Given that some medications have shown adverse effects on sperm quality, there might be a transgenerational concern. We aim at examining the association between drugs proposed to treat COVID-19 when taken by the father around conception and any pre-term birth or major birth defects in offspring in a nation-wide cohort study using Danish registry data. Offspring whose father filled at least one prescription of the following medications in the 3 months preceding conception were considered exposed: chloroquine, hydroxychloroquine, losartan, azithromycin, naproxen, dexamethasone and prednisone. Results For azithromycin and naproxen, large numbers of offspring were exposed (> 1800 offspring), and we found no association with adverse birth outcomes. For chloroquine, losartan and dexamethasone, exposure was intermediate (~ 900 offspring), and there was no statistically significant association with birth defects. For hydroxychloroquine and prednisone, exposure was limited (< 300 offspring). Our evidence suggests that azithromycin and naproxen are safe with respect to pre-term birth and birth defects. For the other drugs investigated larger exposures are needed for conclusive statements.

Teaching Enterprise — Can it Be Done in Large Groups?

2003 ◽  
Vol 17 (1) ◽  
pp. 37-44
Author(s):  
Raymond D. Donnelly
Keyword(s):  
Market Research ◽  
Business Plan ◽  
Management Programme ◽  
First Year ◽  
Large Numbers ◽  
Commercial Sources ◽  
To Come ◽  
The University ◽  
Large Groups

This paper reports on work carried out in the School of Management at Heriot-Watt University in Edinburgh. Following a wide-ranging review of the first-year management programme, a module on enterprise was introduced. As part of that module students had to compete in a game, the object of which was to come up with a business idea, conduct market research and present a business plan and proposal to a panel of judges. The number of students was 225 in year one but reached around 500 within five years. The module has generated many good ideas and has attracted sponsorship from commercial sources. As yet the university has been unable to take the ideas further. It is possible that enterprise can be learned by people in large numbers, but perhaps universities are not the places in which to attempt such work.

A field study of reproductive interactions in Bosca's newt, Triturus boscai

1995 ◽  
Vol 16 (4) ◽  
pp. 357-374 ◽  
Author(s):  
Manuel Mouta Faria
Keyword(s):  
Field Study ◽  
Natural Population ◽  
Wild Population ◽  
Short Circuit ◽  
The Other ◽  
Courtship Behaviour ◽  
Laboratory Studies ◽  
Free Living ◽  
Spermatophore Transfer ◽  
Sexual Interference

AbstractObservations of a free-living population of Bosca's newt, Triturus boscai, show that courtship behaviour in nature is similar to the behaviour observed in earlier laboratory studies. Complementary evidence was obtained on the behaviour called flick, which is sometimes inserted at the end of the static display phase, and may be viewed as an equivalent behaviour to the retreat display of the other small-bodied newt species. Sexual interference in the natural population was mainly caused by males. Two characteristic male behaviour patterns were recognised, the waiting position and the push-tail. Females tend to withdraw from situations of interference. Courtship sequences solely consisting of orientation and spermatophore transfer phases, so-called short-circuit sequences, may be interpreted as a male strategy to avoid the very severe male-to-male interference that exists in a wild population.

THE SOCIAL COSTS OF OBESITY: A NON-REACTIVE FIELD STUDY

1980 ◽  
Vol 8 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Peter L. Benson ◽  
Drew Severs ◽  
John Tatgenhorst ◽  
Nancy Loddengaard
Keyword(s):  
Public Health ◽  
Field Study ◽  
Graduate School ◽  
Social Costs ◽  
The Other ◽  
Cover Letter ◽  
The Social ◽  
Reactive Field ◽  
Health Administrators ◽  
Field Setting

The major purposes of this study were to investigate whether devaluation of obese persons, a phenomenon demonstrated exclusively in laboratory settings using reactive measures, generalizes to a nonreactive field setting. Seventy public health administrators were asked, via the mail, to help a college junior assess her chances of getting into graduate school and finding employment in this field. Subjects received a cover letter, a standard résumé∼, and a questionnaire. A picture of the student was affixed to some of the résumés. Subjects were randomly assigned to one of three picture conditions: obese, normal, or no picture. Forty-six percent of the questionnaires were returned. Significantly fewer forms were returned in the obese condition than in the normal and no picture conditions. On both the graduate school and employment questionnaire items, forecasts were considerably more pessimistic than in the other two picture conditions. Implications of these findings are discussed.

A field study of competition and interaction between Lemna minor and Lemna trisulca

1989 ◽  
Vol 67 (10) ◽  
pp. 2904-2911 ◽  
Author(s):  
Alexander L. McIlraith ◽  
Gordon G. C. Robinson ◽  
Jennifer M. Shay
Keyword(s):  
Life History ◽  
Field Study ◽  
Key Words ◽  
Field Experiments ◽  
Lemna Minor ◽  
Survey Methods ◽  
The Other ◽  
Transplant Experiment ◽  
Replacement Series ◽  
Vegetative Biomass

Field experiments and survey methods were used to assess competition and interaction between Lemna minor L. and Lemna trisulca L. at Delta Marsh, Manitoba. Sites were dominated by one or the other species or codominated by both. Replacement series experiments predicted codominance of L. minor and L. trisulca in an unshaded eutrophic site but predicted L. minor dominance when run for a longer time. Similar experiments conducted in a shaded eutrophic site predicted L. minor dominance. Addition series experiments showed that intraspecific and interspecific competition occurred in the unshaded site. In a eutrophic unshaded ditch, high densities of L. minor suppressed L. trisulca. In a eutrophic shaded site, high densities of L. minor and green algae inhibited L. trisulca, and in a sunny, less eutrophic site high density of each species inhibited the other. In a transplant experiment, L. minor biomass in shaded enclosures approached that found naturally in two shaded sites. Lemna trisulca persisted when shaded. Vegetative biomass trends in an unshaded eutrophic marsh ditch indicated spring and fall L. trisulca dominance and summer L. minor dominance. Shaded eutrophic sites were dominated by L. minor, whereas a less eutrophic site was dominated by L. trisulca. A model is developed to explain dominance patterns, and seasonal life-history responses are considered. Key words: Lemna, duckweed, competition, interaction, resources, light, nutrients.

Beta-Blocker Attenuates Cardiotoxicity Related Anthracycline Usage

2021 ◽  
Vol 104 (8) ◽  
pp. 1389-1392
Keyword(s):  
Side Effects ◽  
Cancer Therapy ◽  
Mortality Rate ◽  
Cancer Patients ◽  
Beta Blocker ◽  
The Other ◽  
Large Numbers ◽  
Therapy Effectiveness ◽  
Chemotherapy Agents

To summarize the recent trials and studies of the role of beta-blocker on the treatment for cancer patients treated with anthracycline to decrease morbidity and mortality rate. Good management of cancer will result in large numbers of cancer survivors. On the other hand, cancer therapy also has side effects, one of which is cardiotoxicity. Cardiotoxicity could reduce therapy effectiveness, hence, increase disease progression and mortality rate. Anthracyclines is one of the chemotherapy agents with cardiotoxicity as a side effect. Beta-blocker has the ability to reduce cardiotoxicity due to anthracyclines usage. Keywords: Beta-blocker; Cardiotoxicity; Anthracyclines

Photoreception of Paramecium cilia: localization of photosensitivity and binding with anti-frog-rhodopsin IgG

1991 ◽  
Vol 99 (1) ◽  
pp. 67-72
Author(s):  
Y. Nakaoka ◽  
R. Tokioka ◽  
T. Shinozawa ◽  
J. Fujita ◽  
J. Usukura
Keyword(s):  
Membrane Potential ◽  
Polyclonal Antibody ◽  
The Other ◽  
Paramecium Bursaria ◽  
Potential Response ◽  
Light Stimulation ◽  
Intact Cells ◽  
Other Hand ◽  
Immunocytochemical Studies ◽  
Antibody Labeling

Paramecium bursaria is photosensitive and accumulates in a lighted area. The cells can be deciliated by a brief suspension in dilute ethanol. Both intact and deciliated cells showed depolarization in response to light stimulation by a step-increase from dark to above 0.7 mW cm-2 (550 nm). On the other hand, after a step-increase to below 0.4 mW cm-1, intact cells showed hyperpolarization, while the deciliated cells showed no change in membrane potential. This difference in membrane potential response between ciliated and deciliated cells suggests that both somatic and ciliary structures are photosensitive. In our search for the photoreceptive molecules, a polyclonal antibody induced in rabbits against frog rhodopsin was found to cross-react with a 63x10(3) Mr protein of P. bursaria, by immunoelectrophoresis. Immunocytochemical studies showed that the antibody labeling was localized on both the ciliary and the somatic membranes. These results raise the possibility that P. bursaria may contain a rhodopsin-like protein as a photoreceptor molecule.

scholarly journals Distribution of Bacillus thuringiensis subsp. israelensis in Soil of a Swiss Wetland Reserve after 22 Years of Mosquito Control

2011 ◽  
Vol 77 (11) ◽  
pp. 3663-3668 ◽  
Author(s):  
Valeria Guidi ◽  
Nicola Patocchi ◽  
Peter Lüthy ◽  
Mauro Tonolla
Keyword(s):  
Spatial Distribution ◽  
Bacillus Thuringiensis ◽  
Confidence Interval ◽  
Quantitative Pcr ◽  
Mosquito Control ◽  
Soil Samples ◽  
Sampling Point ◽  
Content Type ◽  
Large Numbers ◽  
Continuous Accumulation

ABSTRACTRecurrent treatments withBacillus thuringiensissubsp.israelensisare required to control the floodwater mosquitoAedes vexansthat breeds in large numbers in the wetlands of the Bolle di Magadino Reserve in Canton Ticino, Switzerland. Interventions have been carried out since 1988. In the present study, the spatial distribution of restingB. thuringiensissubsp.israelensisspores in the soil was measured. TheB. thuringiensissubsp.israelensisconcentration was determined in soil samples collected along six transects covering different elevations within the periodically flooded zones. A total of 258 samples were processed and analyzed by quantitative PCR that targeted an identical fragment of 159 bp for theB. thuringiensissubsp.israelensis cry4Aaandcry4Bagenes.B. thuringiensissubsp.israelensisspores were found to persist in soils of the wetland reserve at concentrations of up to 6.8 log per gram of soil. Continuous accumulation due to regular treatments could be excluded, as the decrease in spores amounted to 95.8% (95% confidence interval, 93.9 to 97.7%). The distribution of spores was correlated to the number ofB. thuringiensissubsp.israelensistreatments, the elevation of the sampling point, and the duration of the flooding periods. The number ofB. thuringiensissubsp.israelensistreatments was the major factor influencing the distribution of spores in the different topographic zones (P< 0.0001). These findings indicated thatB. thuringiensissubsp.israelensisspores are rather immobile after their introduction into the environment.

scholarly journals Note: Recording of Some Beetles in Honey Bee Colonies

2018 ◽  
Vol 51 (2) ◽  
pp. 85-90 ◽  
Author(s):  
H.F. Abou-Shaara ◽  
M.E. Ahmad ◽  
J. Háva
Keyword(s):  
Honey Bee ◽  
Honey Bees ◽  
The Other ◽  
Beetle Species ◽  
Large Numbers ◽  
The World ◽  
Bee Bread ◽  
Small Hive Beetles ◽  
Bee Colonies ◽  
Different Parts

Abstract Honey bees are very valuable to human. These social insects contribute in the pollination of many crops. Also, the products from honey bee colonies have many nutritional and medicinal benefits. Thus, keeping honey bees are very valuable and can be considered as source of income to many families. There are many diseases and pests that attack honey bee colonies. The pests attack bee colonies include: hornets, wax moths, bee-eater birds, and beetles. Such challenges can impact the survival and productivity of honey bee colonies. In this study, some beetle species belong to Fam. Nitidulidae, Dermestidae and Mycetophagidae were detected in honey bee colonies in Egypt, during spring. Despite the presence of many beetle species in the agricultural environment, only few species preferred the invasion of the colonies for feeding. These beetles do not attack stages of honey bees. They only feed on stored pollen or bee bread, especially those fallen on the bottom of the beehives. This is an alarm to follow the feeding behavior and distribution of these beetles. These beetles’ species can be considered as potential pests to weak honey bee colonies, housed in old or damaged beehives. The presence of large numbers of these beetles in weak colonies may disturb the activities of the bees and may passively impact the survival of the colonies. Listing these beetles is very important to better understanding the interaction between honey bees and beetles. On the other side, small hive beetles were not detected in the colonies. These beetles are currently one of the major problems facing honey bees in different parts of the world. This study confirms the absence of small hive beetles from Egypt.

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