scholarly journals Hypoglycin A in Cow’s Milk—A Pilot Study

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 381
Author(s):  
Mandy Bochnia ◽  
Jörg Ziegler ◽  
Maren Glatter ◽  
Annette Zeyner
Keyword(s):  
Safety Assessment ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Milk Samples ◽  
Filling Station ◽  
Bulk Milk ◽  
Sycamore Maple ◽  
Carry Over ◽  
The Individual

Hypoglycin A (HGA) originating from soapberry fruits (litchi, and ackee) seeds or seedlings from the sycamore maple (SM) tree (related to Sapindaceae) may cause Jamaican vomiting sickness in humans and atypical myopathy in horses and ruminants. A possible transfer into dairy cow’s milk cannot be ruled out since the literature has revealed HGA in the milk of mares and in the offal of captured deer following HGA intoxication. From a study, carried out for another purpose, bulk raw milk samples from four randomly selected dairy farms were available. The cows were pastured in the daytime. A sycamore maple tree was found on the pasture of farm No. 1 only. Bulk milk from the individual tank or milk filling station was sampled in parallels and analyzed for HGA by LC-ESI-MS/MS. Measurable concentrations of HGA occurred only in milk from farm No. 1 and amounted to 120 and 489 nmol/L. Despite low and very variable HGA concentrations, the results indicate that the ingested toxin, once eaten, is transferred into the milk. However, it is unknown how much HGA the individual cow ingested during grazing and what amount was transferred into the bulk milk samples. As a prerequisite for a possible future safety assessment, carry-over studies are needed. Furthermore, the toxins’ stability during milk processing should also be investigated as well.

Longitudinal Analysis of the Microbiological Quality of Raw Cow's Milk Samples Collected from Three Small Family Dairy Farms in Mexico Over a 2-Year Period

2019 ◽  
Vol 82 (12) ◽  
pp. 2194-2200 ◽  
Author(s):  
DIANA RIOS-MUÑIZ ◽  
JORGE F. CERNA-CORTES ◽  
CATALINA LOPEZ-SAUCEDO ◽  
ERIKA ANGELES-MORALES ◽  
MIRIAM BOBADILLA-del VALLE ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Microbiological Quality ◽  
Dairy Farms ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
E Coli ◽  
Milk Samples ◽  
Three Samples ◽  
Raw Cow’S Milk

ABSTRACT In Mexico, the total milk production that family dairy farms (FDF) contribute is ca. 35%, but this milk is not evaluated for microbiological quality. Forty percent of the milk and dairy products consumed by Mexicans is unpasteurized. In total, 24 raw cow's milk samples from three FDF (one sample per each season from each FDF for two sequent years) were characterized for the presence of food quality indicator organisms, Staphylococcus aureus, Salmonella enterica, Listeria monocytogenes, and Mycobacterium spp., by standard procedures. Escherichia coli presence was also evaluated by a direct count method and diarrheagenic E. coli (DEC) by molecular methods. On the basis of Mexican guidelines for raw milk entering production, 42% of samples exceeded the aerobic mesophilic bacteria limits. A total of 83% raw milk samples were positive for total coliforms, 54% for fecal coliforms, and 46% for E. coli. Forty-three E. coli isolates were selected and characterized for the presence of 11 DEC loci; of theses, 40 isolates were negative for all DEC loci, and 3 isolates, all collected from the same sample, were Shiga toxin 2 (stx2) positive and O157 antigen negative, and one stx2 isolate was resistant to 6 of the 16 antibiotics tested. None of the 24 raw milk samples were positive for Salmonella enterica, L. monocytogenes, or staphylococcal enterotoxin. S. aureus was isolated from nine samples, of which only three samples harbored resistant isolates. From three samples, four nontuberculous mycobacterial isolates were recovered (Mycobacteroides chelonae, Mycobacteroides porcinum, and two Mycobacteroides abscessus). All four isolates produced biofilm and had sliding motility, and three isolates (M. porcinum and two M. abscessus) were resistant to the two antibiotics tested (clarithromycin and linezolid). FDF provide raw milk to a large proportion of the Mexican population, but its consumption could be harmful to health, emphasizing the need to implement national microbiological quality guidelines for raw milk intended for direct human consumption. HIGHLIGHTS

Enumeration and Confirmation of Aeromonas hydrophila, Aeromonas caviae, and Aeromonas sobria Isolated from Raw Milk and Other Milk Products in Northern Greece

1999 ◽  
Vol 62 (5) ◽  
pp. 463-466 ◽  
Author(s):  
DIMITRIOS S. MELAS ◽  
DEMETRIOS K. PAPAGEORGIOU ◽  
ANTONIOS I. MANTIS
Keyword(s):  
Raw Milk ◽  
Cow’S Milk ◽  
Aeromonas Species ◽  
Northern Greece ◽  
Cow's Milk ◽  
Milk Samples ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Feta Cheese ◽  
Ewe's Milk

A total of 138 raw cow's and 57 raw ewe's milk samples; 80 pasteurized cow's milk samples; 39 Anthotyros cheese, 36 Manouri cheese, and 23 Feta cheese samples; and 15 rice pudding samples were examined for the presence and any countable population of Aeromonas species. Twenty-two (15.9%) of the 138 cow's milk samples analyzed were contaminated with A. hydrophila. In 13 of these samples, populations of 3.0 × 102 to 5.0 × 103 CFU/ml were counted in starch ampicillin agar (SAA). Eighteen cow's milk samples (13.0%) were contaminated with A. caviae, and in eight of these samples, populations of 2.0 × 102 to 3.0 × 103 CFU/ml were counted in SAA. Five cow's milk samples (3.6%) were contaminated with A. sobria, and in two of these samples, populations of 2.5 × 103 and 5.0 × 103 CFU/ml were counted in SAA. Eleven cow's milk samples (7.9%) were contaminated with other Aeromonas spp. not classified. Eight (14.0%) of the 57 ewe's milk samples analyzed were contaminated with A. hydrophila. In these samples, populations of 5.0 × 102 to 5.0 × 103 CFU/ml were counted in SAA. Six ewe's milk samples (10.5%) were contaminated with A. caviae, and populations of 1.5 × 102 to 1.0 × 103 CFU/ml were counted in SAA. Two ewe's milk samples (3.5%) were contaminated with A. sobria, and populations counted in SAA were 5.0 × 102 and 1.0 × 103 CFU/ml. Four samples (7.0%) were contaminated with other Aeromonas spp. not classified. A. hydrophila was recovered in 4 (10.2%) and 3 (8.3%) of the Anthotyros and Manouri cheese samples analyzed, respectively, but no countable populations were noted in SAA. None of the pasteurized milk, Feta cheese, and rice pudding samples yielded Aeromonas spp. The results of this work indicate that motile Aeromonas are common in raw milk in Greece. Also, the presence of A. hydrophila in the whey cheeses Anthotyros and Manouri indicates that postprocessing contaminations of these products with motile Aeromonas may occur during production.

A survey on the occurrence of aflatoxin M1 in raw milk samples in Bafq and Bahabad, Iran

2021 ◽  
Author(s):  
Vahid Safavizadeh ◽  
Mozhgan Mojkar
Keyword(s):  
Aspergillus Parasiticus ◽  
Raw Milk ◽  
National Standard ◽  
Aflatoxin M1 ◽  
Cow’S Milk ◽  
Elisa Method ◽  
Cow's Milk ◽  
Milk Samples ◽  
The City

Aflatoxins are a group of mycotoxins mostly produced by the fungi called Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomium. Aflatoxin M1 (AFM1) is the major metabolite of aflatoxin B1 and is a hepatotoxic and carcinogenic toxin. The aim of this study was to determine the level of contamination of cow's milk with aflatoxin M1 in Bafq and Bahabad. For this study, samples of raw cow's milk were collected randomly from milk collection centers around the city of Bafq and Bahabad from March to April. The determination of aflatoxin M1 levels was based on the ELISA method. Contamination was observed in 100% of milk samples. According to the results of the study, the rate of contamination with aflatoxin M1 in 43.3% of milk samples was above the acceptable level (50 ng/L) in Iranian national standard. It is concluded that further monitoring of milk production should be carried out in the spring and winter seasons.

scholarly journals Isolation and Molecular Identification and Antimicrobial Susceptibility of Providencia spp. from Raw Cow’s Milk in Baghdad, Iraq

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Nagham Mohammed Ayyal Al‐Gburi
Keyword(s):  
Antimicrobial Susceptibility ◽  
Raw Milk ◽  
Diffusion Method ◽  
Cow’S Milk ◽  
Biochemical Tests ◽  
Street Vendors ◽  
Cow's Milk ◽  
Milk Samples ◽  
Biolog Gn ◽  
Raw Cow’S Milk

A total of sixty raw milk samples were collected from (street vendors and shops) from Baghdad city, Iraq. The samples were inoculated into peptone water and, then, subcultured onto McConkey agar and Blood agar. Identification of isolates was confirmed by microscopic examination, cultural characteristic, biochemical tests, Vitek (VITEK®2 system), and Biolog GN substrate reactions followed by 16S rRNA and specific genes sequencing. Of 60 raw cow’s milk samples, Providencia spp. were identified only in 4 samples (6.67%) and P. rettgeri was the most common, 2/4 (50%), followed by P. stuartii and P. vermicola, 1/4 (25%) . Antimicrobial susceptibility tests were conducted against ten antibiotics by the disc diffusion method. All Providencia isolates showed multidrug resistance (MDR), and the absolute resistant was 100% to tetracycline, erythromycin, and doxycycline and 50% against ampicillin\sulbactam and amoxicillin/clavulanic acid. They were highly susceptible (100%) to trimethoprim, imipenem, and chloramphenicol. These findings indicate that milk might be contaminated with Providencia spp. leading to transmission to humans causing poisoning, diarrhea, and other infections. This is the first study of isolated Providencia spp. from raw cow’s milk.

scholarly journals Staphylococcus aureus of raw cow's milk

2021 ◽  
Vol 23 (102) ◽  
pp. 53-59
Author(s):  
M. D. Kukhtyn ◽  
Y. V. Horiuk ◽  
V. Z. Salata ◽  
V. T. Klymyk ◽  
N. M. Vorozhbit ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Milk Samples ◽  
Before And After ◽  
Pure Cultures ◽  
Almost All

The presence of Staphylococcus aureus in raw milk is considered a direct danger to consumers. Almost all types of dairy products normalize the amount of Staphylococcus aureus in 1 g (cm3) of product, due to their ability to produce thermostable enterotoxins. The aim of the study was to investigate the role of incision of teats of healthy and sick mastitis cows and milking equipment for irradiation of cow's milk with Staphylococcus aureus. Sanitation of milking equipment in farms was carried out with alkaline detergents based on sodium hypochlorite and acid based on phosphoric acid. Blood agar with 5 % sodium chloride was used to isolate staphylococci from washes and milk. Species identification of pure cultures of staphylococci was performed using RapID Staph Plus kits. At the same time before and after milking the skin of teats with Udder Wash and Udder Forte reduces the incidence of Staphylococcus aureus to 4.1 ± 0.3 % of cases. Quantitative determination of staphylococci, including golden on the skin of the teats revealed a decrease in the latter from 283.5 ± 21.2 to 27.6 ± 2.1 CFU/cm3 flush after milking. It has been established that the number of staphylococci, including golden, on the skin of teats increases sharply under subclinical or clinical ointment. Thus, it was found that in the subclinical form of staphylococcal mastitis from the skin of teats isolated S. aureus in the amount of 790.6 ± 65.4 CFU/cm3 of wash. At the same time, in the clinical form of mastitis, the amount of S. aureus increased, on average to 8 thousand CFU/cm3 of flush. It was found that under the condition of sanitary treatment of milking equipment with alternate use of alkaline and acid detergents and disinfectants, only 25 % of washes were staphylococci, and gold – up to 5 % of samples. It was found that in 43.7 ± 3.9 % of raw milk samples Staphylococcus aureus was not detected in 1 cm3. The number of milk samples containing Staphylococcus aureus up to 100 CFU/cm3 was 39.1 ± 3.3 %, and 17.2 ± 1.4 % of milk samples were contaminated with Staphylococcus aureus in the amount of 101 to 200 CFU/cm3. Therefore, the presence of more than 200 CFU/cm3 in freshly milked Staphylococcus aureus in milk indicates the ineffectiveness of antimastitis measures, skin remediation of teats and sanitation of milking equipment.

scholarly journals EVALUATION OF THE MICROBIAL LOAD AND IDENTIFICATION OF POSSIBLE SOURCES OF CONTAMINATION OF RAW COW’S MILK IN JOANNA, BLACK BUSH POLDER, REGION 6, GUYANA

2020 ◽  
Vol 8 (5) ◽  
pp. 22-31
Author(s):  
Roshnie Singh ◽  
Dharamdeo Singh ◽  
Phillip Da Silva ◽  
Gomathinayagam Subramanian
Keyword(s):  
Simple Random Sampling ◽  
Raw Milk ◽  
High Water ◽  
Microbial Load ◽  
Cow’S Milk ◽  
Aerobic Bacteria ◽  
Cow's Milk ◽  
Milk Samples ◽  
Milk Contamination ◽  
Raw Cow’S Milk

Milk is one of the main sources of nutrients to animals, but due to its high water activity, it serves as an ideal medium for the growth of microorganisms. The purpose of this research was to evaluate the microbial load in raw cow’s milk and to identify the possible sources of raw milk contamination in Joanna. Fifteen (15) raw milk samples were collected randomly according to the simple random sampling method and farmers were interviewed. Raw milk samples were analysed for Escherichia coli and aerobic bacteria. From the 15 samples of raw milk, contamination with E. coli (93.33%) and aerobic bacteria (100%) was observed. Also, several factors, milk handling practices, unhygienic practices, extensive farming systems, and lack of general zoonoses exposure practices and awareness, predisposed raw milk to microbial contamination. This research concluded that the quality of milk was unsatisfactory since it was heavily contaminated with bacteria. It is recommended that there should be an increase in the agriculture extension education and awareness programme to help improve milk quality.

scholarly journals Evaluation of Microbiological and Compositional Quality of Raw Cow’s Milk (Household and Bulk) in Lankapura, Polonnaruwa, Sri Lanka

2021 ◽  
Vol 3 (1) ◽  
pp. 166-175
Author(s):  
G. D. D. K. Gunasena ◽  
B. A. M. P. Siriwardhana
Keyword(s):  
Milk Production ◽  
Statistical Significance ◽  
Milk Quality ◽  
Cow’S Milk ◽  
Small Scale ◽  
Cow's Milk ◽  
Milk Samples ◽  
Bulk Milk ◽  
Compositional Quality

Purpose: At present, in Lankapura, there is a tremendous development in dairy sector. But milk quality issues were raised by the milk consumers and producers who collected milk from Lankapura. Although, measures have been taken to increase the milk production, the quality of milk has not been thoroughly evaluated in area. Research methodology: This study was carried out to evaluate the microbiological, compositional quality of cow’s milk collected from small scale farmers (25) and bulk milk tanks (25 samples) in the study area and to collect data about knowledge of farmers on clean milk production practices. Findings: According to the study, acidity, and average level of bacterial count of household milk production were 0.229±0.029%, 6.193±0.311 log10 CFU/ml respectively and for bulk milk production were 0.294± 0.020%, 6.6427±0.322 log10 CFU/ml respectively. The mean percentage of specific gravity, protein, fat, lactose and SNF for bulk milk samples were 1.029g/ml, 2.740%±0.0866, 4.120%±0.8065, 4.040%±0.1581, 7.6476%±0.34219 respectively and for household’s milk samples were 1.028g/ml, 2.792% ±0.1706, 4.360%±0.7314, 4.032%±0.1973, 7.5716%±0.33726 respectively. When considering management practices of farmers, only 38% farmers cleaned the milking place. Only 28% of farmers maintained proper records. The results indicated that microbiological and compositional quality (except milk fat) were not in acceptable level for both household and bulk milk samples. For microbiological quality, there was a high statistical significance of bulk milk samples than the households. Limitations: The quality of the milk was poor due to unhygienic practices and poor knowledge. Sample numbers were limited in this study, due to limited time period for this study. Larger individual coliform count could not be conducted due to limited manpower and laboratory resources. Originality/ value: As long term solutions such as farmer education programmes, incentive based milk quality systems can be recommended.

scholarly journals Biofilm Formation Ability and Presence of Adhesion Genes among Coagulase-Negative and Coagulase-Positive Staphylococci Isolates from Raw Cow’s Milk

Pathogens ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 654
Author(s):  
Joanna Gajewska ◽  
Wioleta Chajęcka-Wierzchowska
Keyword(s):  
Biofilm Formation ◽  
Raw Milk ◽  
Cow’S Milk ◽  
Genetic Determinants ◽  
Coagulase Negative Staphylococci ◽  
Cow's Milk ◽  
Milk Samples ◽  
Slime Production ◽  
Study Results ◽  
Staphylococcus Spp

The capacity for biofilm formation is one of the crucial factors of staphylococcal virulence. The occurrence of biofilm-forming staphylococci in raw milk may result in disturbances in technological processes in dairy factories as well as the contamination of finished food products. Therefore, this study aimed to determine the prevalence and characteristics of staphylococcal biofilm formation in raw milk samples and to explore the genetic background associated with biofilm formation in those isolates. The material subjected to testing included 30 cow’s milk samples acquired from farms in the central part of Poland. A total of 54 staphylococcal strains were isolated from the samples, of which 42 were classified as coagulase-negative (CoNS) staphylococci belonging to the following species: S. haemolyticus, S. simulans, S. warneri, S. chromogenes, S. hominis, S. sciuri, S. capitis, S. xylosus and S. saprophyticus, while 12 were classified as S. aureus. The study examined the isolates’ capacity for biofilm formation and the staphylococcal capacity for slime production and determined the presence of genetic determinants responsible for biofilm formation, i.e., the icaA, icaD, bap and eno and, additionally, among coagulase-negative staphylococci, i.e., the aap, bhp, fbe, embP and atlE. Each tested isolate exhibited the capacity for biofilm formation, of which most of them (79.6%) were capable of forming a strong biofilm, while 5.6% formed a moderate biofilm, and 14.8% a weak biofilm. A capacity for slime production was demonstrated in 51.9% isolates. Most of the tested staphylococcal strains (90.7%) had at least one of the tested genes. Nearly half (47.6%) of the CoNS had the eno gene, while for S. aureus, the eno gene was demonstrated in 58.3% isolates. The frequency of the bap gene occurrence was 23.8% and 25% in CoNS strains and S. aureus, respectively. The fbe gene was demonstrated in only three CoNS isolates. The presence of the icaA was only demonstrated in CoNS strains (24.1%), while the icaD was found in both CoNS strains (21.4%) and S. aureus (100%). Among the CoNS, the presence of the embP (16.7%), aap (28.6%) and atlE (23.8%) was demonstrated as well. The obtained study results indicate that bacteria of the Staphylococcus spp. genus have a strong potential to form a biofilm, which may pose a hazard to consumer health.

Prevalence and Distribution of Arcobacter spp. in Raw Milk and Retail Raw Beef

2012 ◽  
Vol 75 (8) ◽  
pp. 1474-1478 ◽  
Author(s):  
A. H. SHAH ◽  
A. A. SALEHA ◽  
M. MURUGAIYAH ◽  
Z. ZUNITA ◽  
A. A. MEMON
Keyword(s):  
Dominant Species ◽  
Raw Milk ◽  
Cow’S Milk ◽  
First Report ◽  
Cow's Milk ◽  
Milk Samples ◽  
Goat’S Milk ◽  
Arcobacter Butzleri ◽  
Goat's Milk

A total of 106 beef samples which consisted of local (n = 59) and imported (n = 47) beef and 180 milk samples from cows (n = 86) and goats (n = 94) were collected from Selangor, Malaysia. Overall, 30.2% (32 of 106) of beef samples were found positive for Arcobacter species. Imported beef was significantly more contaminated (46.80%) than local beef (16.9%). Arcobacter butzleri was the species isolated most frequently from imported (81.8%) and local (60%) beef, followed by Arcobacter cryaerophilus in local (33.3%) and imported (18.2%) beef samples. Only one local beef sample (10%) yielded Arcobacter skirrowii. Arcobacter species were detected from cow's milk (5.8%), with A. butzleri as the dominant species (60%), followed by A. cryaerophilus (40%), whereas none of the goat's milk samples were found positive for Arcobacter. This is the first report of the detection of Arcobacter in milk and beef in Malaysia.

scholarly journals Occurrence and antimicrobial sensitivity in staphylococci isolated from goat, sheep and cow’s milk

2011 ◽  
Vol 59 (3) ◽  
pp. 209-214 ◽  
Author(s):  
Marcela Vyletělová ◽  
Oto Hanuš ◽  
Renáta Karpíšková ◽  
Zora Šťástková
Keyword(s):  
Animal Origin ◽  
Cow’S Milk ◽  
Biochemical Tests ◽  
Cow's Milk ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Sheep Milk ◽  
Amoxicillin Clavulanic Acid ◽  
The Individual ◽  
Isolated Species

The aim of this study was to compare the sensitivity to selected antibiotics in staphylococci isolated from goat (n = 60), sheep (n = 60) and cow’s milk (n = 120). The individual milk samples were inoculated onto Blood agar cultivated at 36 °C/24 h.The isolated species of staphylococci were identified using biochemical tests, namely STAPHYtest and identification program TNW pro 6.5. We examined the sensitivity of strains to the spectrum of antibiotics, as follows: vancomycin (VA), amoxicillin/clavulanic acid (AMC), penicillin (P), rifampicin (RD), oxacillin (OX), tetracycline (TE), erythromycin (E), chloramphenicol (C), clindamycin (DA), gentamicin (CN), ciprofloxacin (CIP), teicoplanin (TEC), cefoxitin (FOX) and novobiocin (NOV). Altogether, 97 staphylococcal isolates were obtained; 70 from cow’s milk, 11 from goat’s milk and 16 from sheep’ milk.Staphylococcus aureuswas the most frequent species in milk of all animal origin tested, was detected in 54 (45%) cow’s milk, 10 (17%) goat’s and 15 (25%) sheep’ milk samples.S. lentuswas identified only in goat’s and sheep’ milk whereas in cow’s milk there were representation of staphylococcal species as follows:S. haemolyticus(n = 7),S. chromogenes(n = 2),S. warneri(n = 2),S. xylosus(n = 2),S. epidermidis(n = 2) and unclassified staphylococci (n = 1). The results ofS. aureussensitivity are similar for all tested antibiotics and for all monitored milk: No resistance to vancomycin, rifampicin, chloramphenicol and teicoplanin was recorded in obtainedS. aureusisolates whereas the resistance to ciprofloxacin was found out most often.

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