scholarly journals Larvicidal Activities against Aedes aegypti of Supernatant and Pellet Fractions from Cultured Bacillus spp. Isolated from Amazonian Microenvironments

2021 ◽  
Vol 6 (2) ◽  
pp. 104
Author(s):  
Ricardo M. Katak ◽  
Elerson M. Rocha ◽  
Juan C. Oliveira ◽  
Veranilce A. Muniz ◽  
Marta R. Oliveira ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Bacterial Strains ◽  
Rna Sequences ◽  
16S Rna ◽  
Bacillus Spp ◽  
Primary Vector ◽  
Larvicidal Activities ◽  
First Time ◽  
New Strategies

The Aedes aegypti mosquito is the primary vector of Dengue, Chikungunya and Zika causing major problems for public health, which requires new strategies for its control, like the use of entomopathogenic microorganisms. In this study, bacteria from various Amazonian environments were isolated and tested for their pathogenicity to A. aegypti larvae. Following thermal shock to select sporulated Bacillus spp., 77 bacterial strains were isolated. Molecular identification per 16S RNA sequences revealed that the assembled strains contained several species of the genus Bacillus and one species each of Brevibacillus, Klebsiella, Serratia, Achromobacter and Brevundimonas. Among the isolated Bacillus sp. strains, 19 showed larvicidal activity against A. aegypti. Two strains of Brevibacillus halotolerans also displayed larvicidal activity. For the first time, larvicidal activity against A. aegypti was identified for a strain of Brevibacillus halotolerans. Supernatant and pellet fractions of bacterial cultures were tested separately for larvicidal activities. Eight strains contained isolated fractions resulting in at least 50% mortality when tested at a concentration of 5 mg/mL. Further studies are needed to characterize the active larvicidal metabolites produced by these microorganisms and define their mechanisms of action.

scholarly journals New Purinyl-Steroid and Other Constituents from the Marine Fungus Penicillium brefeldianum ABC190807: Larvicidal Activities against Aedes aegypti

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Zong-Min Hou ◽  
Sen-Quan Yu ◽  
Min Tao ◽  
Cai-Bei Xia ◽  
Yu-Lian Xia ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
2D Nmr ◽  
Marine Fungus ◽  
Mangrove Sediment ◽  
Health Issues ◽  
Etoac Extract ◽  
The Third ◽  
Larvicidal Activities ◽  
Third Instar Larvae

Mosquitoes historically threatened human health; the major mosquito-related global health issues include malaria, dengue fever, yellow fever, and Zika, as well as several other vector-borne outbreaks. Here, the EtOAc extract of the mangrove sediment fungus Penicillium brefeldianum ABC190807 exerted larvicidal activities against the third instar larvae of Aedes aegypti with an LC50 of 0.089 mg/mL. One new purinyl-steroid (ergosta-4,6,8(14),22-tetraen-3-(6-amino-9H-purin-9-yl) (1)), along with six (2–7) known compounds, were isolated from the EtOAc extract of Penicillium brefeldianum ABC190807. Structures of the compounds were elucidated via 1D/2D NMR and HR-ESI-MS data. Respective spectral data were compared with those of known compounds. Among all compounds whose larvicidal activity against the third instar larvae of Aedes aegypti was evaluated, compounds 2 and 7 showed larvicidal activity with respective LC50 values of 0.452 and 0.337 mg/mL.

scholarly journals Antioxidant and Larvicidal Activities of Tephrosia Egregia Sandw against Aedes Aegypti

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ângela M. C. Arriaga ◽  
Jefferson Q. Lima ◽  
Jackson N. E Vasconcelos ◽  
Maria C. F. de Oliveira ◽  
Telma L. G. Lemos ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Hexane Extract ◽  
High Antioxidant Activity ◽  
Larvicidal Activities ◽  
Low Activity ◽  
Ethyl Acetate Extracts

The antioxidant and larvicidal activities of Tephrosia egregia extracts and its major component, dehydrorotenone, were studied. High antioxidant activity was found for dehydrorotenone and methanol and ethyl acetate extracts from roots and stems, respectively. Among the tested extracts, the hexane extract from stems showed potent larvicidal activity (LC50 12.88 ± 0.64) while low activity was found for dehydrorotenone.

scholarly journals Antitumor and Aedes aegypti Larvicidal Activities of Essential Oils From Piper klotzschianum, P. hispidum, and P. arboreum

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986393 ◽  
Author(s):  
Rafaely N. Lima ◽  
Adauto S. Ribeiro ◽  
Gilvandete M.P. Santiago ◽  
Cinara O. d´S. Costa ◽  
Milena B. Soares ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Larvicidal Activity ◽  
Chemical Constituents ◽  
Promyelocytic Leukemia ◽  
Germacrene D ◽  
Larvicidal Activities

The chemical constituents of essential oils extracted by hydrodistillation from Piper klotzschianum, P. hispidum, and P. arboreum, collected from the remaining Atlantic forest in Sergipe State, were characterized and submitted to cytotoxic and larvicidal activity assays. The major constituents identified (stems, fresh, and dried leaves) were ( E)-caryophyllene (16.8%) and bicyclogermacrene (21.6%). Germacrene D (22.8%) was identified only in P. klotzschianum and P. hispidum. A high percentage of β-pinene (27.2%) and α-pinene (7.2%) were present in P. klotzschianum stems and δ-3-carene in the fresh and dried leaves of P. hispidum (17.4% and 19.1%, respectively). Piper klotzschianum showed the best cytotoxic activity, inhibiting human hepatocellular carcinoma (27.3 µg/mL), human promyelocytic leukemia (33.8 µg/mL), and murine melanoma (37.9 µg/mL) cell lines. Larvicidal activity was also tested and the essential oil from the dried leaves of P. klotzschianum was the most potent against Aedes aegypti larvae, with an LC50 value of 122.4 µg/mL.

scholarly journals Larvicidal effect of dried leaf extracts from Pinus caribaea against Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae)

2009 ◽  
Vol 42 (4) ◽  
pp. 373-376 ◽  
Author(s):  
Luiz Alberto Kanis ◽  
Rodrigo Damian Antonio ◽  
Ériston Paes Antunes ◽  
Josiane Somariva Prophiro ◽  
Onilda Santos da Silva
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Lignin Content ◽  
World Health ◽  
Pinus Caribaea ◽  
Leaf Extracts ◽  
Reduced Pressure ◽  
Lignin Concentration ◽  
Health Organization ◽  
First Time

In this study, the larvicidal activity of dried leaf extracts from Pinus caribaea Morelet against Aedes aegypti was evaluated for the first time. Pinus caribaea extracts were obtained by macerating dried leaves in alkaline hydroethanol, ethanol and acetone solutions followed by evaporation under reduced pressure. The lignin content was quantified using the thioglycolic acid complexation method. Lethality bioassays (LC50 and LC90) were carried out in accordance with the recommendations of the World Health Organization. The results showed that the acetone extract from Pinus caribaea was more active, and that larvicidal activity was associated with lignin concentration.

scholarly journals Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti

2021 ◽  
Vol 12 ◽  
Author(s):  
Marta Rodrigues de Oliveira ◽  
Ricardo de Melo Katak ◽  
Gilvan Ferreira da Silva ◽  
Osvaldo Marinotti ◽  
Olle Terenius ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Fungal Strains ◽  
Amazon Rain Forest ◽  
Vector Management ◽  
Global Increase ◽  
Larvicidal Activities

The global increase in diseases transmitted by the vector Aedes aegypti, new and re-emerging, underscores the need for alternative and more effective methods of controlling mosquitoes. Our aim was to identify fungal strains from the Amazon rain forest that produce metabolites with larvicidal activity against Aedes aegypti. Thirty-six fungal strains belonging to 23 different genera of fungi, isolated from water samples collected in the state of Amazonas, Brazil were cultivated. The liquid medium was separated from the mycelium by filtration. Medium fractions were extracted with ethyl acetate and isopropanol 9:1 volume:volume, and the mycelia with ethyl acetate and methanol 1:1. The extracts were vacuum dried and the larvicidal activity was evaluated in selective bioassays containing 500 μg/ml of the dried fungal extracts. Larval mortality was evaluated up to 72 h. None of the mycelium extracts showed larvicidal activity greater than 50% at 72 h. In contrast, 15 culture medium extracts had larvicidal activity equal to or greater than 50% and eight killed more than 90% of the larvae within 72 h. These eight extracts from fungi belonging to seven different genera (Aspergillus, Cladosporium, Trichoderma, Diaporthe, Albifimbria, Emmia, and Sarocladium) were selected for the determination of LC50 and LC90. Albifimbria lateralis (1160) medium extracts presented the lowest LC50 value (0.268 μg/ml) after 24 h exposure. Diaporthe ueckerae (1203) medium extracts presented the lowest value of LC90 (2.928 μg/ml) at 24 h, the lowest values of LC50 (0.108 μg/ml) and LC90 (0.894 μg/ml) at 48 h and also at 72 h (LC50 = 0.062 μg/ml and LC90 = 0.476 μg/ml). Extracts from Al. lateralis (1160) and D. ueckerae (1203) showed potential for developing new, naturally derived products, to be applied in integrated vector management programs against Ae. aegypti.

scholarly journals Larvicidal, adulticidal, and oviposition-deterrent activity of Piper betle L. essential oil to Aedes aegypti

2019 ◽  
Vol 12 (3) ◽  
pp. 367-371 ◽  
Author(s):  
Riesna Martianasari ◽  
Penny Humaidah Hamid
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Dengue Fever ◽  
Larvicidal Activity ◽  
Mosquito Control ◽  
Activity Index ◽  
Piper Betle ◽  
Primary Vector ◽  
Aegypti Population ◽  
Deterrent Activity

Background and Aim: Aedes aegypti is a primary vector of many arthropod-borne diseases. One of the diseases, dengue fever, is an endemic disease in Indonesia causing high mortalities for decades. There are no preventive and specific treatments for dengue so far. Therefore, prevention of this disease largely depends on the mosquito control. Since resistance to chemical insecticides occurred worldwide, the study on alternate and new mosquito insecticides are mandatory. This study aimed to demonstrate the effect of essential oil from P. betle L. in the larval and adult stages, as well as its influence on oviposition activity of A. aegypti mosquito. Materials and Methods: P. betle efficacy was evaluated in various stages of A. aegypti development. For the larvicidal activity, larvae instar III stage was used. Adulticidal assay in this experiment was performed using newly emerged A. aegypti. For oviposition assay, mated A. aegypti was tested for their responses to P. betle-treated and non-treated ovitraps. Results: P. betle L. - adulticide activity was effective with a concentration of 2.5 μl/ml, caused 100% mortality within 15-30 min. Larvicide activity was observed after 1 h, 24 h, and 48 h post-treatment with LC50183, 92.7, and 59.8 ppm and LC90> 637, 525, and 434.7 ppm, respectively. Oviposition activity index was −0.917 in 1000 ppm. In addition, the eggs number of A. aegypti oviposition with 100 ppm of essential oil P. betle L. was 5 times lower than the control. Conclusion: This study demonstrated clearly that essential oil derived from P. betle L. potentially acts as alternate bioinsecticide to control A. aegypti population. The application can be varied or combined in different stages of mosquito development.

scholarly journals Larvicidal Activity against Aedes Aegypti of Essential Oils from Northeast Brazil

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Patrícia L. Lavor ◽  
Gilvandete M. P. Santiago ◽  
Roberto W. da Silva Gois ◽  
Leôncio M. de Sousa ◽  
Gabrieli da P. Bezerra ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Northeast Brazil ◽  
Artemisia Vulgaris ◽  
Cymbopogon Flexuosus ◽  
Larvicidal Activities ◽  
Third Instar Larvae

The larvicidal activities of essential oils from the leaves of Artemisia vulgaris L., Cymbopogon flexuosus(Nees ex Steud.) Wats. and Piper tuberculatum Jacq. were evaluated using third-instar larvae of Aedes aegypti. The essential oils were obtained by hydrodistillation and analyzed by GC and GC-MS. The essential oil of P. tuberculqatum had the lowest LC50 value (106.3 ± 2.2 μg/mL), followed by that of A. vulgaris(114.1 ± 1.7 μg/mL) and C. flexuosus(121.6 ± 0.8 μg/mL). The results show that these essential oils may be potent sources of natural larvicides.

scholarly journals Chemical Constituents and Insecticidal Activity of Rollinia leptopetala (Annonaceae)

2008 ◽  
Vol 3 (10) ◽  
pp. 1934578X0800301
Author(s):  
Ângela M.C. Arriaga ◽  
Edinilza M.A. Feitosa ◽  
Telma L.G. Lemos ◽  
Gilvandete M.P. Santiago ◽  
Jefferson Q. Lima ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Spectral Data ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Nmr Data ◽  
First Time

The present work describes the isolation of dehydrodiscretamine, discretamine, liriodenine, lichexanthone, molvizarin, rolliniastatin-1, annonacin and bullatacin from roots of Rollinia leptopetala R E Fries. The structures were established after analysis of their NMR spectral data including 2D NMR experiments. With the exception of discretamine, the presence of these compounds in R. leptopetala and the NMR data of dehydrodiscretamine are reported for the first time. The CH2Cl2 extract of the roots demonstrated high larvicidal activity against Aedes aegypti, with an LC50 value of 1.44±2.0 ppm.

scholarly journals Screening of some weed extracts for ovicidal and larvicidal activities against dengue vector Aedes aegypti

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Sarika Torawane ◽  
Ramnath Andhale ◽  
Radhakrishna Pandit ◽  
Digambar Mokat ◽  
Samadhan Phuge
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Plant Extracts ◽  
Insect Pests ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Egg Hatching ◽  
Ms Analysis ◽  
Ethanol Extracts ◽  
Larvicidal Activities

Abstract Background The use of plant-based formulations is an easy and economical method to control insect pests. The objectives of the present study were to evaluate ovicidal and larvicidal potential of ethanolic leaf extracts of four plants, Cyathocline purpurea, Blumea lacera, Neanotis lancifolia, and Neanotis montholonii, and their effects on gut histology of Aedes aegypti (Diptera: Culicidae) larvae. We identified the phytochemicals present in the ethanolic extracts of these plants by LC-MS analysis. The eggs and larvae of A. aegypti were exposed to four concentrations (0.05, 0.1, 0.2, and 0.3 mg/mL) of crude ethanol extracts for different time durations. We observed egg hatching for 48 h, mortality, and gut histology of the 3rd instar larvae after 24, 48, and 72 h of treatments. Results LC-MS analysis revealed the presence of various active compounds such as alkaloids, tannins, saponins, and flavonoids in the ethanol extracts of all these plants. The extracts of all plants showed ovicidal activity. The extracts of C. purpurea showed the highest larvicidal activity (LC50 at 72 h = 0.088 mg/mL) followed by B. lacera (LC50 at 72 h = 0.758 mg/mL) while N. lancifolia and N. montholonii extracts did not show significant larvicidal activity. All plant extracts affected gut morphology in concentration and treatment duration-dependent manner. The plant extracts used in the present study have ovicidal properties and affected the gut histology of A. aegypti larvae. Conclusion These results suggest that C. purpurea has the highest larvicidal activity against A. aegypti larvae and can be further evaluated for application purposes.

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