scholarly journals New Purinyl-Steroid and Other Constituents from the Marine Fungus Penicillium brefeldianum ABC190807: Larvicidal Activities against Aedes aegypti

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Zong-Min Hou ◽  
Sen-Quan Yu ◽  
Min Tao ◽  
Cai-Bei Xia ◽  
Yu-Lian Xia ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
2D Nmr ◽  
Marine Fungus ◽  
Mangrove Sediment ◽  
Health Issues ◽  
Etoac Extract ◽  
The Third ◽  
Larvicidal Activities ◽  
Third Instar Larvae

Mosquitoes historically threatened human health; the major mosquito-related global health issues include malaria, dengue fever, yellow fever, and Zika, as well as several other vector-borne outbreaks. Here, the EtOAc extract of the mangrove sediment fungus Penicillium brefeldianum ABC190807 exerted larvicidal activities against the third instar larvae of Aedes aegypti with an LC50 of 0.089 mg/mL. One new purinyl-steroid (ergosta-4,6,8(14),22-tetraen-3-(6-amino-9H-purin-9-yl) (1)), along with six (2–7) known compounds, were isolated from the EtOAc extract of Penicillium brefeldianum ABC190807. Structures of the compounds were elucidated via 1D/2D NMR and HR-ESI-MS data. Respective spectral data were compared with those of known compounds. Among all compounds whose larvicidal activity against the third instar larvae of Aedes aegypti was evaluated, compounds 2 and 7 showed larvicidal activity with respective LC50 values of 0.452 and 0.337 mg/mL.

scholarly journals Larvicidal Activity against Aedes Aegypti of Essential Oils from Northeast Brazil

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Patrícia L. Lavor ◽  
Gilvandete M. P. Santiago ◽  
Roberto W. da Silva Gois ◽  
Leôncio M. de Sousa ◽  
Gabrieli da P. Bezerra ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Northeast Brazil ◽  
Artemisia Vulgaris ◽  
Cymbopogon Flexuosus ◽  
Larvicidal Activities ◽  
Third Instar Larvae

The larvicidal activities of essential oils from the leaves of Artemisia vulgaris L., Cymbopogon flexuosus(Nees ex Steud.) Wats. and Piper tuberculatum Jacq. were evaluated using third-instar larvae of Aedes aegypti. The essential oils were obtained by hydrodistillation and analyzed by GC and GC-MS. The essential oil of P. tuberculqatum had the lowest LC50 value (106.3 ± 2.2 μg/mL), followed by that of A. vulgaris(114.1 ± 1.7 μg/mL) and C. flexuosus(121.6 ± 0.8 μg/mL). The results show that these essential oils may be potent sources of natural larvicides.

scholarly journals Larvicidal Activity of Ethyl Acetate Extract of Derris elliptica Root against the Third-instar Larvae of Cypermethrin-Resistant Aedes aegypti Offspring

2021 ◽  
Author(s):  
Sayono Sayono ◽  
Risyandi Anwar ◽  
Didik Sumanto
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Ethyl Acetate Extract ◽  
Larval Mortality ◽  
Laboratory Strain ◽  
The Third ◽  
Resistant Strains ◽  
Four Levels ◽  
Third Instar Larvae

Background: Derris elliptica extracts have a high larvicidal potential against the laboratory strain of Aedes aegypti larvae, but the effect on offspring larvae of pyrethroid-resistant strains of the species is lack understood. This study aimed to determine the larvicidal activity of the ethyl acetate extract of tuba root against the third-instar larvae of the Cypermethrin-resistant Ae. aegypti offspring. Methods: The experimental study occupied four levels of ethyl acetate extract of D. elliptica namely 10, 25, 50, and 100 ppm, and each level was four times replicated. As many as twenty of healthy third-instar larvae, offspring of Cypermethrin-resistant Ae. aegypti were subjected to each experiment group. Larval mortality rate and lethal concentration 50% subject (LC50) were calculated after 24 and 48 hours of exposure time. Results: Mortality of larvae increased directly proportional to the increase of extract concentration. Larval mortality rates after 24 and 48 hours of exposure were 40–67.5% and 62.5–97.5%, and LC50 were 34.945 and 6.461ppm, respectively. Conclusion: The ethyl acetate extract of D. elliptica has the high effectiveness larvicidal potential against the thirdinstar larvae, offspring of the Cypermethrin-resistant Ae. aegypti. Isolation of the specific compound is necessarily done to obtain the active ingredient for larvicide formulation.

scholarly journals Restricticin B from the Marine Fungus Penicillium janthinellum and Its Inhibitory Activity on the NO Production in BV-2 Microglia Cells

2020 ◽  
Author(s):  
Byeonung-Kyu Choi ◽  
Shin-Young Park ◽  
Song-Hee Jo ◽  
Dong-Kug Choi ◽  
Phan Thi Hoai Trinh ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Optical Rotation ◽  
2D Nmr ◽  
Marine Fungus ◽  
No Production ◽  
Spectroscopy Data ◽  
Penicillium Janthinellum ◽  
Etoac Extract ◽  
Data Comparison ◽  
Nmr Data

A new compound containing a triene, a tetrahydropyran ring and glycine ester functionalities, restricticin B (1), together with four known compounds (2-5) were obtained from the EtOAc extract of the marine fungus Penicillium janthinellum. The planar structure of 1 was determined by detailed analyses of MS, 1D and 2D NMR data. The relative and absolute configurations of 1 were established via analyses of NOESY spectroscopy data, comparison of optical rotation values with those of restricticin derivatives reported and electronic circular dichroism (ECD). All the compounds were screened for their anti-neuroinflammatory effects in lipopolysaccharide (LPS)-induced BV-2 microglia cells. Restricticin B (1) and N-acetyl restricticin (2) exhibited anti-neuroinflammatory effects by suppressing the production of pro-inflammatory mediators in activated microglial cells.

scholarly journals Chemical Composition and Larvicidal Activity of Eugenia triquetra Essential Oil from Venezuelan Andes

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Flor D. Mora ◽  
Jorge L. Avila ◽  
Luis B. Rojas ◽  
Rosslyn Ramírez ◽  
Alfredo Usubillaga ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Chemical Constituents ◽  
Ms Analysis ◽  
Third Instar Larvae

The chemical constituents of the essential oil obtained by hydrodistillation of the leaves of Eugenia triquetra O. Berg, collected in Táchira State, Venezuela, were identified by GC-MS analysis. Twenty-six components, which made up 88.5% of the oil, were identified. The major constituents were linalool (17.5%), limonene (16.9%), α-pinene (11.6%), β-pinene (8.7%), and p-cymene (3.7%). The essential oil was tested against third-instar larvae of Aedes aegypti, showing a LC50 value of 64.8 ± 5.6 ppm.

scholarly journals Antimicrobial activity of natural compounds from sponge – derived fungus Aspergillus flocculosus 01NT.1.1.5

2020 ◽  
Vol 16 (4) ◽  
pp. 729-735
Author(s):  
Phan Thi Hoai Trinh ◽  
Tran Thi Thanh Van ◽  
Bui Minh Ly ◽  
Byeoung Kyu Choi ◽  
Hee Jae Shin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Culture Medium ◽  
Antimicrobial Agents ◽  
2D Nmr ◽  
Marine Fungus ◽  
Antimicrobial Drugs ◽  
Etoac Extract ◽  
E Coli ◽  
Chemical Structures ◽  
Pathogenic Microbes

The Aspergillus fungi have been an important source of natural products that are useful for exploration in medicine, agriculture and industry. In our continuous investigation to search for new antimicrobial agents from marine-derived fungi, one new phomaligol A2 (1), together with three known compounds, wasabidienone E (2), aspertetranone D (3) and mactanamide (4), were obtained from the EtOAc extract of the culture medium of the marine-derived fungus Aspergillus flocculosus (A. flocculosus) 01NT.1.1.5 isolated from the sponge Stylissa sp. at Nhatrang Bay, Vietnam. Their chemical structures were elucidated by analysis of 1D and 2D NMR and mass spectroscopic data, as well as by comparison of the corresponding data to those previously reported in the literature. Furthermore, the aim of this study was also to evaluate the antimicrobial activity of these compounds against pathogenic microbes including Escherichia coli (E. coli) ATCC 25922, Pseudomonas aeruginosa (P. aeruginosa) ATCC 27853, Staphylococcus aureus (S. aureus) ATCC 25923, Bacillus cereus (B. cereus) ATCC 11778, Streptococcus faecalis (S. faecalis) ATCC 19433, Listeria monocytogenes (L. monocytogenes) ATCC 19111, and Candida albicans (C. albicans) ATCC 10231. Among the compounds, 1-3 were inhibitory on the growth of the yeast C. albicans with minimum inhibitory concentration (MIC) value of 16 μg/mL, which was more potent than amoxicillin and cefotaxime (MIC > 256 μg/mL), antimicrobial drugs as positive references. Moreover, compounds 1-4 were also found to be active against other pathogens including P. aeruginosa and S. faecalis with MIC values of 16 μg/mL and 32 μg/mL, respectively. Compound 4 had no inhibitory activity against L. monocytogenes, whereas compounds 1-3 had ability to against this strain with MICs of 32 to 64 μg/mL. Four of tested compounds exhibited antibacterial activity against B. cereus and E. coli with MIC values of 64-128 μg/mL. This is the first report about these compounds with antimicrobial activity obtained from marine fungus A. flocculosus isolated in Vietnam.

scholarly journals Pemanfaatan Jeruk Purut (Citrus Hystrix) Sebagai Biolarvasida

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Titik Lestari
Keyword(s):  
Essential Oil ◽  
Literature Review ◽  
Aedes Aegypti ◽  
Research Methods ◽  
Chemical Compound ◽  
Mosquito Vector ◽  
The Third ◽  
Chemical Content ◽  
Lethal Time ◽  
Third Instar Larvae

Absctract: Citrus hystrix, Biolarvicides. Insectisides and pestisides were used as larvicides to control mosquito vector Aedes aegypti particularly. Some research suggested there had been a larvae resistance to pesticides. Biolarvicides with basic ingredients plant was another alternative that can be used. Lime (Citrus hystrix) was one of plants that often used as biolarvicides, both lits fruit and leaves. This paper aimed to know about chemical compound in lime and its potential as biolarvicidal. Research methods was literature review from several research journals. The results showed that the presence of coumpounds were essential oil, flavonoids, saponins, terpeesn, limonoids, citronellal, linalool, citronellol, acetate citronelil, kariofilin and geraniol. LC50 of lime leaves as biolarvicides againts the third instar larvae of Aedes aegypti was 279.882 ppm, biolarvicides oxicity values (LC90) was 4000 ppm with the lethal time (LT90) was 12.67 hours. Based on results of this study concluded that the presence of the chemical content of Citrus hystrix plant potential as biolarvicides.

scholarly journals Antioxidant and Larvicidal Activities of Tephrosia Egregia Sandw against Aedes Aegypti

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ângela M. C. Arriaga ◽  
Jefferson Q. Lima ◽  
Jackson N. E Vasconcelos ◽  
Maria C. F. de Oliveira ◽  
Telma L. G. Lemos ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Hexane Extract ◽  
High Antioxidant Activity ◽  
Larvicidal Activities ◽  
Low Activity ◽  
Ethyl Acetate Extracts

The antioxidant and larvicidal activities of Tephrosia egregia extracts and its major component, dehydrorotenone, were studied. High antioxidant activity was found for dehydrorotenone and methanol and ethyl acetate extracts from roots and stems, respectively. Among the tested extracts, the hexane extract from stems showed potent larvicidal activity (LC50 12.88 ± 0.64) while low activity was found for dehydrorotenone.

scholarly journals Larvicidal Activity against Aedes Aegypti L. (Diptera: Culicidae) of Essential Oils of Lippia Species from Brazil

2006 ◽  
Vol 1 (7) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
Gilvandete M. P. Santiago ◽  
Telma L. G. Lemos ◽  
Otília D. L. Pessoa ◽  
Ângela M. C. Arriaga ◽  
Francisco J. A. Matos ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Third Instar Larvae

The larvicidal activity of the essential oils from leaves of five species of Lippia (L. alba, L. aff. gracilis, L. gracilis, L. microphylla and L. nodiflora) was studied on third instar larvae of Aedes aegypti. The larvae were exposed to serial concentrations from 500 to 12.5 μg/mL of the essential oils. The results indicate that the oil of L. gracilis has a higher larvicidal activity with a LC50 of 26.3 μg/mL The results suggest the use of these essential oils as natural larvicidal agents.

scholarly journals Antitumor and Aedes aegypti Larvicidal Activities of Essential Oils From Piper klotzschianum, P. hispidum, and P. arboreum

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986393 ◽  
Author(s):  
Rafaely N. Lima ◽  
Adauto S. Ribeiro ◽  
Gilvandete M.P. Santiago ◽  
Cinara O. d´S. Costa ◽  
Milena B. Soares ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Cytotoxic Activity ◽  
Larvicidal Activity ◽  
Chemical Constituents ◽  
Promyelocytic Leukemia ◽  
Germacrene D ◽  
Larvicidal Activities

The chemical constituents of essential oils extracted by hydrodistillation from Piper klotzschianum, P. hispidum, and P. arboreum, collected from the remaining Atlantic forest in Sergipe State, were characterized and submitted to cytotoxic and larvicidal activity assays. The major constituents identified (stems, fresh, and dried leaves) were ( E)-caryophyllene (16.8%) and bicyclogermacrene (21.6%). Germacrene D (22.8%) was identified only in P. klotzschianum and P. hispidum. A high percentage of β-pinene (27.2%) and α-pinene (7.2%) were present in P. klotzschianum stems and δ-3-carene in the fresh and dried leaves of P. hispidum (17.4% and 19.1%, respectively). Piper klotzschianum showed the best cytotoxic activity, inhibiting human hepatocellular carcinoma (27.3 µg/mL), human promyelocytic leukemia (33.8 µg/mL), and murine melanoma (37.9 µg/mL) cell lines. Larvicidal activity was also tested and the essential oil from the dried leaves of P. klotzschianum was the most potent against Aedes aegypti larvae, with an LC50 value of 122.4 µg/mL.

scholarly journals Extracts of Amazonian Fungi With Larvicidal Activities Against Aedes aegypti

2021 ◽  
Vol 12 ◽  
Author(s):  
Marta Rodrigues de Oliveira ◽  
Ricardo de Melo Katak ◽  
Gilvan Ferreira da Silva ◽  
Osvaldo Marinotti ◽  
Olle Terenius ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Fungal Strains ◽  
Amazon Rain Forest ◽  
Vector Management ◽  
Global Increase ◽  
Larvicidal Activities

The global increase in diseases transmitted by the vector Aedes aegypti, new and re-emerging, underscores the need for alternative and more effective methods of controlling mosquitoes. Our aim was to identify fungal strains from the Amazon rain forest that produce metabolites with larvicidal activity against Aedes aegypti. Thirty-six fungal strains belonging to 23 different genera of fungi, isolated from water samples collected in the state of Amazonas, Brazil were cultivated. The liquid medium was separated from the mycelium by filtration. Medium fractions were extracted with ethyl acetate and isopropanol 9:1 volume:volume, and the mycelia with ethyl acetate and methanol 1:1. The extracts were vacuum dried and the larvicidal activity was evaluated in selective bioassays containing 500 μg/ml of the dried fungal extracts. Larval mortality was evaluated up to 72 h. None of the mycelium extracts showed larvicidal activity greater than 50% at 72 h. In contrast, 15 culture medium extracts had larvicidal activity equal to or greater than 50% and eight killed more than 90% of the larvae within 72 h. These eight extracts from fungi belonging to seven different genera (Aspergillus, Cladosporium, Trichoderma, Diaporthe, Albifimbria, Emmia, and Sarocladium) were selected for the determination of LC50 and LC90. Albifimbria lateralis (1160) medium extracts presented the lowest LC50 value (0.268 μg/ml) after 24 h exposure. Diaporthe ueckerae (1203) medium extracts presented the lowest value of LC90 (2.928 μg/ml) at 24 h, the lowest values of LC50 (0.108 μg/ml) and LC90 (0.894 μg/ml) at 48 h and also at 72 h (LC50 = 0.062 μg/ml and LC90 = 0.476 μg/ml). Extracts from Al. lateralis (1160) and D. ueckerae (1203) showed potential for developing new, naturally derived products, to be applied in integrated vector management programs against Ae. aegypti.

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