Abstract. Inland waters impart considerable influence on nutrient cycling and budget estimates across local, regional and global scales, whilst anthropogenic pressures, such as rising populations and the appropriation of land and water resources, are undoubtedly modulating the flux of carbon (C), nitrogen (N), and phosphorus (P) between terrestrial biomes to inland waters, and the subsequent flux of these nutrients to the marine and atmospheric domains. Here, we present a two year biogeochemical record (October 2011–December 2013) at bi-weekly sampling resolution for the lower Sabaki River, Kenya, and provide estimates for suspended sediment and nutrient export fluxes from the Athi-Galana-Sabaki (A-G-S) river basin under pre-dam conditions, and in light of the approved construction of the Thwake Multi-purpose Dam on the Athi River. Erratic seasonal variation was typical for most parameters, with generally poor correlation between discharge and material concentrations and stable isotopic signatures of C (δ13C) and N (δ15N). Although high total suspended matter (TSM) concentrations are reported here (up to ~ 3.8 g L−1), peak concentrations of TSM rarely coincided with peak discharge. The contribution of particulate organic C (POC) to the TSM pool indicates a wide bi-annual variation in suspended sediment load from OC-poor (0.3 %) to OC-rich (14.9 %), with the highest %POC occurring when discharge is 80 % of the total load for TSM (~ 86 %), POC (~ 89 %), DOC (~ 81 %), PN (~ 89 %) and TPP (~ 82 %), with > 50 % of each fraction exported during the long wet season (March–May). Our estimated sediment yield of 85 Mg km−2 yr−1 is relatively low on the global scale and is considerably less than the recently reported average sediment yield of ~ 630 Mg km−2 yr−1 for African river basins. Regardless, sediment and OC yields were all at least equivalent or greater than reported yields for the neighbouring and flow-regulated Tana River. Rapid pulses of heavily 13C-enriched POC coincided with peak concentrations of PN, ammonium, CH4 and low dissolved oxygen saturation, lead to the suggestion that large mammalian herbivores (e.g. hippopotami) may mediate the delivery of C4 organic matter to the river during the dry season. Given recent projections for increasing dissolved nutrient export from African rivers, as well as planned flow regulation on the Athi River, these first estimates of material fluxes from the Sabaki River provide base-line data for future research initiatives assessing anthropogenic perturbation of the A-G-S river basin.