scholarly journals A New Thioalkalivibrio sp. Strain Isolated from Petroleum-Contaminated Brackish Estuary Sediments: A New Candidate for Bio-Based Application for Sulfide Oxidation in Halo-Alkaline Conditions

Water ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1385
Author(s):  
Simone Becarelli ◽  
Salvatore La China ◽  
Alla Lapidus ◽  
Andrey Prijibelski ◽  
Dmitrii Polev ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Type Strain ◽  
Petroleum Hydrocarbon ◽  
Sulfide Oxidation ◽  
Sodium Concentration ◽  
Industrial Applications ◽  
Total Petroleum Hydrocarbon ◽  
Alkaline Conditions ◽  
Optimal Efficiency ◽  
H2s Emission

A new halo-alkaline sulfur-oxidising bacterial strain was isolated from brackish estuary sediments contaminated by total petroleum hydrocarbon. The isolate was classified as a new strain of Thioalkalivibrio sulfidiphilus sp., showing a higher capability of adaptation to pH and a higher optimal sodium concentration for growth, when compared to Thioalkalivibrio sulfidiphilus sp. HL-EbGr7, type strain of the species. The strain was capable to grow in saline concentrations up to 1.5 M Na+ and pH up to 10. The genome of the new isolate was sequenced and annotated. The comparison with the genome of Thioalkalivibrio sulfidiphilus sp. HL-EbGr7 showed a duplication of an operon encoding for a putative primary sodium extruding pump and the presence of a sodium/proton antiporter with optimal efficiency at halo-alkaline conditions. The new strain was able to oxidize sulfide at halo-alkaline conditions at the rate of 1 mmol/mg-N/h, suitable for industrial applications dedicated to the recovery of alkaline scrubber for H2S emission absorption and abatement.

Flame Photometry Characterization Procedure for Sodium Content in Brazilian Bentonites for Nanotechnology Applications

2019 ◽  
Vol 12 (2) ◽  
pp. 122-129
Author(s):  
Hellen S. Santos ◽  
Karine L. Buarque da Silva ◽  
Ariel E. Zanini ◽  
Danilo S. Coelho ◽  
Marcelo Embiruçu ◽  
...  
Keyword(s):  
Control Process ◽  
Sodium Concentration ◽  
High Water ◽  
Sodium Content ◽  
Industrial Applications ◽  
Exchange Capacity ◽  
Sodium Cation ◽  
Flame Photometry ◽  
Logarithmic Scale ◽  
Average Value

Background: Brazilian bentonites have a low sodium concentration in their interlayer structure. This is a problem with most of the industrial applications that demand the characteristics of sodium bentonites. Objective: As a solution for this limitation, sodium carbonate is added to in natura clays, promoting ion exchange between other interlayer cations with sodium. Methods: A process was used based on the principle of Stokes’ Law (BR Patent 10 2013 016298). For this, we used four glass columns in series, in which a high water flow was considered to obtain purified clays. It was proposed as a simple, fast and economical method for sodium determination that was developed and applied in bentonites by flame photometry. The equipment calibration was performed with a NaCl standard solution in concentrations between 80 and 110 ppm. The bentonites in the suspension were separated by means of centrifugation, being thus analyzed by a flame photometer. Results: The samples were prepared according to the manufacturer’s specifications to contain detectable amounts of sodium by means of flame photometry. A resulting linear relationship between the average value readings versus sodium standard content solution (both in ppm) was obtained by the logarithmic scale, as expected. Conclusion: The procedure allowed to define a method that could be used in the sodification control process, thus making it possible to differentiate the sodium cation content in terms of the value of cation exchange capacity (CEC) from bentonite. X-ray analysis of in natura and the sodified clays showed nanostructural differences related to interlayer distance.

scholarly journals Physicochemical Indices of Phyto Remediated Hydrocarbon Polluted Soil and the Effect of Bio-nutrient Amendment

2021 ◽  
pp. 14-33
Author(s):  
J. O. Dasetima-Altraide ◽  
D. N. Ogbonna ◽  
T. K. S. Abam ◽  
A. E. Gobo
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbon ◽  
Polluted Soil ◽  
Total Petroleum Hydrocarbon ◽  
Plant Roots ◽  
Phyllanthus Amarus ◽  
Cyperus Esculentus ◽  
Percentage Reduction ◽  
Nutrient Supplements ◽  
Test Plants

Aim: To assess the Physicochemical indices of Phytoremediated Crude Oil polluted amended soil using grass plant Cyperus esculentus (Cyp) and Phyllanthus amarus (Phy). Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: Rivers State University demonstration farmland in Nkpolu- Oroworukwo, Mile 3 Diobu area of Port Harcourt, was used for this study. The piece of land is situated at Longitude 4°48’18.50” N and Latitude 6ᵒ58’39.12” E measuring 5.4864 m x 5.1816 m with a total area of 28.4283 square meter. Phytoremediation process monitoring lasted for 240 days; analyses were carried out monthly at 30 days’ interval. Methodology: The study was carried out on Crude Oil Polluted soil (PS) amended with bio-nutrient supplements (Spent Mushroom Substrate (SMS) and selected fungi (Aspergillus niger(AN) andMucor racemosus (MR)) used to stimulate and augment the indigenous microbial population present in a crude oil polluted soil thereby enhancing hydrocarbon reduction in pari per sue with phytoremediation (uptake of Crude oil by test plants) over a period of 240 days. Ten (10) experimental plots (two Control (Unpolluted and polluted soil without amendment) and eight polluted amended/treated plots) employing Randomized Block Design (each having dimensions: 100 x 50 x 30 cm LxBxH); formed and mapped out on agricultural soil and left fallow for 6 days before contamination on the seventh day; after which it was allowed for 21 days for proper contamination and exposure to natural environmental factors (to mimic soil crude oil spill site); thereafter nutrients/organics (biostimulating agents) and bioaugmenting organisms were applied. Baseline studies were carried out on soil profile before and after contamination, major parameters monitored and assayed were Total Petroleum Hydrocarbon (TPH) uptake by plant roots and stem, Polycyclic Aromatic Hydrocarbons (PAHs) and TPH reduction in soil. Other physicochemical properties analyzed in the soil from different plots were pH, Electrical Conductivity, Moisture Content, Total Nitrogen, Available Phosphorus, Potassium, Total Organic Carbon, Plant Height, Iron, Lead and Zinc at regular intervals; days 1, 60, 90, 120, 150, 180, 210 & 240. The rate of phytoremediation was estimated from percentage (%) uptake of Total petroleum hydrocarbon (TPH) in plant roots and stem from day 1 -240; while percentage (%) reduction of TPH and PAHs in soil was estimated from day 1 to the residual at day 240. Results: The test plants decreased significant amount of crude oil as revealed in TPH uptake in their roots and Stem. Mean amount and percentage Total Petroleum Hydrocarbon (TPH) uptake by Cyperus esculentus roots and stem were; 152.33±50.34mg/kg, 12.57±4.16% and 201.13±8.80mg/kg, 13.27±0.58% respectively; while that of Phyllanthus amarusroots and stem were 141.50±35.62mg/kg, 11.68±2.94% and 174.44±19.98mg/kg, 11.51±1.32% respectively; revealing higher Uptake of TPH in plant stem than roots. From the initial TPH contamination value of 5503.00mg/kg, it was observed that plots planted with Cyperus esculentus (TPH 5492.75±76.36mg/kg) showed higher reduction of TPH from soil than those planted with Phyllanthus amarus(TPH 5449.72±18.27mg/kg); while PAHs degradation/reduction showed a reverse trend with plots planted with Phyllanthus amarus (PAHs 28.72±2.74mg/kg; 60.46±5.77%) higher than plots planted with Cyperus esculentus s (PAHs 25.77±2.12mg/kg, 54.24±4.47%). Conclusion: Plots planted with Cyperus esculentus showed higher reduction of TPH from soil than those planted with Phyllanthus amarus while PAHs degradation/reduction in plots planted with Phyllanthus amarus was higher than plots planted with Cyperus esculentus. TPH uptake was higher in plant stems than roots; more so, plots amended with nutrient supplements showed significant higher percentage reduction in hydrocarbon in the polluted soil than unamended polluted soil. It is therefore recommended that Cyperus esculentus is a suitable plant species for phytoremediation of crude oil contaminated soil with high TPH value while Phyllanthus amarusis the best option in phytoremediation of polluted soil with high PAHs value, both in combination with bio-nutrient supplement.

scholarly journals Biodegradation Potentials of Aspergillus sydowii and Fusarium lichenicola on Total Petroleum Hydrocarbon in an Oilfield Wastewater in Rivers State

2020 ◽  
pp. 1-7
Author(s):  
Williams, Janet Olufunmilayo ◽  
Aleruchi Owhonka
Keyword(s):  
Mixed Culture ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbon ◽  
Total Petroleum Hydrocarbons ◽  
Aspergillus Sydowii ◽  
Significant Difference ◽  
Set Up ◽  
Rivers State

This study investigated the potential of Aspergillus sydowii and Fusarium lichenicola as mixed cultures in the biodegradation of Total Petroleum Hydrocarbons TPHs in oilfield wastewater. Oilfield wastewater was collected from an onshore oil producing platform and biodegradation of total petroleum hydrocarbons was investigated using standard methods. Fungi were isolated from oilfield wastewater contaminated soils obtained from the vicinity of the oil producing platform. Experimental control set-up and treatment with mixed culture of fungal isolates were periodically analyzed on days 7 and 21 intervals for total petroleum hydrocarbon degradation using Gas Chromatography (GC). The total amount of TPHs on day 1 recorded 381. 871 mg/l.  The amount of TPHs on days 7 and 21 in the mixed culture of fungi was 108.975 mg/l and 21.105 mg/l respectively while TPHs in control was 342.891 mg/l and 240.749 mg/l respectively. There was a significant difference between the mixed culture and the control on days 7 and 21 at p≤0.05. The results therefore revealed actual and significant reduction of TPHs in the mixed culture. In addition, there was clearance of n-alkanes by the mixed culture. This suggests that fungi have great potentials in biodegradation of TPHs and in remediation of TPH contaminated environments.

scholarly journals The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
RIRYN NOVIANTY ◽  
ANNISA HIDAYAH ◽  
SARYONO SARYONO ◽  
AMIR AWALUDDIN ◽  
NOVA WAHYU PRATIWI ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Petroleum Industry ◽  
Degradation Process ◽  
Petroleum Products ◽  
Industrial Applications ◽  
Polluted Soil ◽  
Gas Chromatography Mass Spectrometry ◽  
Terrestrial Environments

Abstract. Novianty R, Saryono, Awaluddin A, Pratiwi NW, Hidayah A, Juliantari E. 2021. The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon. Biodiversitas 22: 5077-5084. One of the major problems in the petroleum industry nowadays is crude oil spills. Riau Province, Sumatra is one of the largest oil producers in Indonesia. Accidental releases of petroleum products are of particular concern to the environment. The process of drilling and refining petroleum generates a large amount of oil sludge. One of the effective technologies used in the waste degradation process is bioremediation using certain microorganisms. The prime objective of the current research was to evaluate the efficiency of fungi consortiums in crude oil degradation in Bumi Siak Pusako-Pertamina Hulu, Indonesia. There are three potential fungi isolates as petroleum hydrocarbon degradation agents with four consortium variations. The parameter values of Optical Density (OD), pH, and diluted CO2 were measured on 0, 4, 8, and 16 days. To evaluate the fungal biodegradation activity using Gas Chromatography-Mass Spectrometry (GC-MS). The result showed that consortium II (KF II) has the highest potential to degrade petroleum hydrocarbon (50.61%). The visual GC-MS examination confirmed a decrease in the peak area for eight hydrocarbon compounds, indicating the efficiency of the fungi in the oil decomposition and dismantling of hydrocarbons. Our findings may provide new information on native fungal resources from chronically contaminated terrestrial environments, and will be useful for petroleum-contaminated bioremediation and other industrial applications.  

scholarly journals MIKROORGANISME PENDEGRADASI TPH (TOTAL PETROLEUM HYDROCARBON) SEBAGAI AGEN BIOREMEDIASI TANAH TERCEMAR MINYAK BUMI (Review Article)

2018 ◽  
Vol 2 (2) ◽  
pp. 35
Author(s):  
Prasetyo Handrianto
Keyword(s):  
Petroleum Hydrocarbons ◽  
Soil Chemistry ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Oil Pollution ◽  
Review Article ◽  
Total Petroleum Hydrocarbon ◽  
Soil Conditions ◽  
Petroleum Pollution ◽  
Ph Optimization

Exploitation and exploration activities will produce sewage sludge and crude oil spills that cause pollution to the environment and upgrading to the environment, biology and soil chemistry. Monitoring of oil pollution conditions on the soil can be done by detection of all hydrocarbon components, or what is called the total petroleum hydrocarbon (TPH). According to its components, this total petroleum hydrocarbon (TPH) can be classified into 3 points, aliphatic, alicyclic, and aromatic. One of the biological efforts that can be used to overcome petroleum pollution is by using bioremediation technology. There are several methods in bioremediation, one of which is the biostimulation method, where the growth of the original hydrocarbon decomposers is stimulated by adding nutrients, oxygen, pH optimization and temperature. Hydrocarbonoclastic microorganisms have characteristic not possessed by other microorganisms, namely their ability to excrete hydroxylase enzymes, which are hydrocarbon oxidizing enzymes, so that these bacteria can degrade petroleum hydrocarbons. Biodegradation can be formed if there is a structural transformation so that cahnges in molecular integrity occur. This process is a series of enzymatic or biochemical reaction that require ideal environmental conditions with the growth and proliferation of microorganisms. Something that need to be known before remediation are pollutants (organic or inorganic), degraded/ not, dangerous/ not, how many pollutants pollute the soil, the ratio of carbon (C), Nitrogen (N), and phophorus (P), soil type, soil conditions (wet dry), and how long pollutants have been deposited in these locations

scholarly journals AnIn SilicoApproach for Evaluating a Fraction-Based, Risk Assessment Method for Total Petroleum Hydrocarbon Mixtures

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nina Ching Y. Wang ◽  
Glenn E. Rice ◽  
Linda K. Teuschler ◽  
Joan Colman ◽  
Raymond S. H. Yang
Keyword(s):  
Risk Assessment ◽  
Petroleum Hydrocarbon ◽  
Environmental Fate ◽  
Assessment Method ◽  
Total Petroleum Hydrocarbon ◽  
Field Analysis ◽  
Chemical Methods ◽  
Risk Assessment Method ◽  
Risk Of Exposure ◽  
Mixture Risk Assessment

Both the Massachusetts Department of Environmental Protection (MADEP) and the Total Petroleum Hydrocarbon Criteria Working Group (TPHCWG) developed fraction-based approaches for assessing human health risks posed by total petroleum hydrocarbon (TPH) mixtures in the environment. Both organizations defined TPH fractions based on their expected environmental fate and by analytical chemical methods. They derived toxicity values for selected compounds within each fraction and used these as surrogates to assess hazard or risk of exposure to the whole fractions. Membership in a TPH fraction is generally defined by the number of carbon atoms in a compound and by a compound's equivalent carbon (EC) number index, which can predict its environmental fate. Here, we systematically and objectively re-evaluate the assignment of TPH to specific fractions using comparative molecular field analysis and hierarchical clustering. The approach is transparent and reproducible, reducing inherent reliance on judgment when toxicity information is limited. Our evaluation of membership in these fractions is highly consistent (̃80% on average across various fractions) with the empirical approach of MADEP and TPHCWG. Furthermore, the results support the general methodology of mixture risk assessment to assess both cancer and noncancer risk values after the application of fractionation.

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