scholarly journals Evidence of Resistance of Heavy Metals from Bacteria Isolated from Natural Waters of a Mining Area in Mexico

Water ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 2766
Author(s):  
Alondra Escamilla-Rodríguez ◽  
Salvador Carlos-Hernández ◽  
Lourdes Díaz-Jiménez

This study focuses on identifying relationships between the content of heavy metals in water and the resistance patterns of different bacteria. Samples from watercourses in one of the most important mining areas in Mexico were collected. Seventy-one bacteria were isolated, and their resistance to Cr, Zn, Cu, Ag, Hg, and Co was studied. The Minimum Inhibitory Concentration range was determined, and a Multiple Metal Resistant index was calculated. After that, 11 isolated bacteria were chosen to estimate kinetic parameters. The obtained results show differences in the behavior of the studied bacteria concerning the presence of heavy metals in the media: (1) without effect, (2) inhibited growth; and (3) considerable inhibited growth. Finally, a Performance Index was proposed to select adequate bacteria for heavy metals removal; five bacteria were selected. Among them, Pseudomonas koreensis was identified as a good candidate for a future biosorption system since these bacteria can stimulate growth in the presence of all the metals tested.

Author(s):  
Shuai Song ◽  
Yuanjie Li ◽  
Lin Li ◽  
Maoyong Liu ◽  
Jing Li ◽  
...  

Mining activities make important contributions to economic growth, but they can also produce massive amounts of solid waste, such as tailings and metal accumulations. Taking the Urad Houqi mining area in Inner Mongolia as the study area, this study systematically assessed the contamination risk of arsenic and heavy metals in the soils of the study area and explored the contamination characteristics in a key polymetallic mining area. For the whole study area, based on the Nemerow comprehensive pollution method, almost half of the investigated sites were contaminated, and the most contaminated site was Urad Houqi Qianzhen Mineral Concentration Co., Ltd. (Bayannaoer, China), a cooperation between the lead and zinc mining industry. The assessment results indicated that Cd and As were the elements of greatest concern, followed by Pb, Cr and Hg. Particularly, for the typical Dongshengmiao mining area, when compared with the GB15618-1995 standard values, As, Zn and Cd posed the most serious contamination threat, while Cr and Ni exhibited clean conditions. In addition, the vertical distribution maps demonstrated that the contents of arsenic and metals in some soil profiles were correlated with sampling depth. Therefore, arsenic and heavy metals pose high threat to soil ecosystems in this area, there is encouragement for some control and remediation measures to be taken into effect.


Author(s):  
Chang Li ◽  
Liugen Zheng ◽  
Chunlu Jiang ◽  
Xing Chen ◽  
Shuaishuai Ding

AbstractCoal gangue is the main pollution source of mining areas. When coal gangue is stacked and recycled, the heavy metal elements contained in it are released by natural weathering and leaching, which causes damage to the surrounding ecological environment. In this study, the leaching and precipitation characteristics of heavy metals in low-sulfur coal gangue under different environmental conditions were simulated by indoor dynamic leaching experiments, which provided a theoretical basis for environmental restoration of the mining area. The conclusions are as follows: higher heavy metal content in low-sulfur coal gangue is associated with greater, leaching of heavy metals; acidic conditions promote the release of heavy metals in low-sulfur coal gangue; and more precipitation is associated with better release of heavy metals from the low-sulfur coal gangue.


2020 ◽  
Vol 29 (1) ◽  
pp. 15-22
Author(s):  
Bayartogtokh Bataa ◽  
Nurmukhammed Burshakhbai ◽  
Altanchimeg Adilbish ◽  
Lkhamjav Gendenpil ◽  
Tserenchimed Sainnokhoi ◽  
...  

In the last decade, there is growing concern about the increase in the number of unknown diseases of pasture livestock due to the natural and anthropogenic factors, such as heavy metals and dust exposure. Thus, it is necessary to measure the concentration of heavy metals in different organs of animals, as well as blood parameters assay and histopathology. We have collected samples from different suspected mining areas, using a random sampling method, and selected 15 goats and 15 sheep. The histopathologic study was performed in necropsy, also macroscopic and microscopic analysis were performed on the diseased animals comparing with healthy animals. In this study, 37.3 % of the sheep and 54.6% of the goat samples were found with gross lesions from different organs, while 24% of sheep and 18.6% of goat organs had microscopic change. Through histo-pathology study, small ruminant lungs of livestock from suspected mining areas were found with inflammatory nodules, while other edible organs were changed in a special transition. In conclusion, we assume the dust exposure from the mining area can be the reason behind the dysfunction. Уул уурхайн, хайгуул олборлолтын бүс орчимд бэлчээрлэж байсан малын эд, эрхтэнд эд судлалын шинжилгээ хийсэн дүнгээс Дэлхийн дулааралт, хуурайшилт болон хүний хүчин зүйлийн (байгаль орчинд халтай технологи бүхий уул уурхайн эрчимтэй үйлдвэрлэл, тээвэрлэлт, эмх замбараагүй олборлолт, хог хаягдал г.м) нөлөөлөлд  Монгол орны бэлчээрийн мал өртсөний улмаас онош нь тодорхойгүй өвчин, төрөл бүрийн хүнд металлын хордлогот эмгэг илэрч, өвчлөх, үхэх, тоо толгой нь хорогдох зэрэг эдийн засгийн хохирол бүхий тохиолдол сүүлийн жилүүдэд нэмэгдэх хандлагатай болсон. Иймд мал амьтны эд эрхтэнд агуулагдаж буй хүнд металлын агууламжийг тодорхойлохын зэрэгцээ биохими болон эд судлалын шинжилгээ хийх шаардлага урган гарсан. Эд судлалын шинжилгээнд төв аймгийн Заамар сумын алтны хайгуул, олборлолт явуулж буй бүс нутаг орчим бэлчээрлэж байсан 6 хонь, 6 ямаа, Дорноговь аймгийн Улаанбадрах, Зүүнбаян  сумын нутаг дэвсгэрт ураны хайгуул, туршилтын олборлолт явуулж буй бүс нутаг болон Айраг сумын жоншны уурхайн олборлолтын бүс нутаг орчим бэлчээрлэж байсан 9 хонь, 9 ямааны эд эрхтнүүдийг хамруулан шинжилгээ хийсэн. Түлхүүр үг: хонь, ямаа,  эд судлалын эмгэгт өөрчлөлт, үлэмж ба бичил бүтэц


2019 ◽  
Vol 11 (2) ◽  
pp. 339 ◽  
Author(s):  
Marcos G. P. de Abreu ◽  
Paulo F. Mendes Filho ◽  
Kaio G. V. Garcia ◽  
José M. T. da Silva Junior ◽  
Gustavo H. da S. Albuquerque ◽  
...  

Soil pollution by heavy metals is mainly caused by poor management of tailings generated by mining during exploration and processing of metals. Given the concern about the soil environment, this study aimed to evaluate the tolerance and effectiveness of isolates of rhizobium strains native to soils of Mn2+-mining areas in association with Mimosa caesalpiniaefolia (Benth.) cultivated under increasing concentrations of Mn2+. Isolates of rhizobium strains, obtained from Mn-mining area, were authenticated and morphologically characterized. Effect of Mn2+ on the growth and nodulation of M. caesalpiniaefolia was subsequently evaluated. We obtained 14 isolates which were similar, differing only in the form of colonies. Mn2+ did not inhibit the symbiotic process between isolates and plant species. The isolates from mining area showed higher efficiency than native strains. All isolates are Mn2+-tolerant, and those from mining area can increase the tolerance of M. caesalpiniaefolia to excess Mn2+ in the soil.


2021 ◽  
Author(s):  
Xun Wang

Abstract In this study, taking a coal mining area as an example, three vegetation restoration modes were designed: Populus L., Ligustrum lucidum Ait., and Amygdalus persica L., and soil and plant samples were collected to determine and evaluate the heavy metals. It was found that all the three modes were effective in eliminating heavy metal pollution in the soil, especially Populus L. and Ligustrum lucidum Ait.; in the soil layer at a depth of 0–20 cm, the content of Cd was the lowest (2.68 mg/kg) in Populus L., and the content of Cr and Pb was the lowest (58.64 mg/kg and 95.36 mg/kg) in Ligustrum lucidum Ait., which was significantly lower than that in the bare land. The evaluation results demonstrated that the pollution under Populus L. and Ligustrum lucidum Ait. modes was moderate. In the aspect of the heavy metal content in plants, the content of Cd was the lowest, and the content of Cr and Pb was high. In the same plant, the content of heavy metals in the leaf was the lowest, followed by the stem and root. The experimental results show that the vegetation restoration mode can relieve the heavy metal pollution, which makes some contributions to solve the ecological restoration problem in coal mining areas.


2021 ◽  
Author(s):  
Zhentian Liang ◽  
Wenjing Zhang ◽  
Yuesuo Yang ◽  
Jincai Ma ◽  
Shuxin Li ◽  
...  

Abstract There is a certain connection between the microbial community and functional flora in rare earth mining areas, but the pollution characteristics and pollution metabolic pathways in the mining area are still unclear. The conditions of heavy metals, rare earth elements, and microorganisms after the mining of rare earth mines were studied in this paper. It was found that after mining, all the sampling areas showed low pH and low total organic carbon(TOC), accompanied by high iron and aluminum concentrations. The development of vegetation is closely related to the development level of microorganisms. In the complex environment of rare earth mining areas, the Proteobacteria showed an absolute competitive advantage. In the process of mine environmental recovery, the relative abundance of Acidobacteria will increase significantly, but with the further development of restoration, the relative abundance of Firmicutes in rare earth mining areas will gradually decrease. There are many genera of bacteria related to the N cycle and heavy metal metabolism in the study area, indicating important metabolic pathways of ammonia nitrogen and heavy metals in rare earth mining areas. At the same time, the study found that there are bacterial genera that promote plant nitrogen fixation in the area, further revealing the nitrogen cycle.


2022 ◽  
Vol 334 ◽  
pp. 08006
Author(s):  
Martí Aliaguilla ◽  
Daniele Molognoni ◽  
Pau Bosch-Jimenez ◽  
Eduard Borràs

Industrial activity has resulted in heavy metals anthropogenic contamination of groundwater, especially in industrial or mining areas. Bioelectrochemical systems (BES) can be used for metals removal and recovery from aqueous solutions. In the framework of GREENER project, double-chamber BES have been adopted to treat groundwater from industrial sites containing copper, nickel and zinc (Cu, Ni and Zn), among other contaminants. Two operation modes, (i) short-circuited microbial fuel cell (MFC), and (ii) power supply driven microbial electrolysis cell (MEC, poisoning the cathode at -0.4 V vs. Ag/AgCl), were studied for metals removal at lab-scale. Two control reactors were run to evaluate metals adsorption on cathodes and membranes, and the effect of anolyte composition. Synthetic water containing different concentrations of Cu, Ni and Zn were treated, and metals removal pathways were studied. MEC and MFC performed similarly and the highest removal efficiencies were 97.1±3.6%, 50.7±6% and 74,5% for Cu, Ni and Zn respectively, from initial concentrations in the range of 1.1-1.5 mM.


2021 ◽  
Author(s):  
Minjie Chen ◽  
Xiaoru Jiang ◽  
Zhansheng Mi ◽  
Yafei Li ◽  
Zhe Wang ◽  
...  

Abstract Background Environmental pollution from rare earth mining areas is of great concern, but the impact on microbial ecology and genomics has received little attention. In this study, the relationship between heavy metals and soil microbial community in the northern rare earth mining area was explored. Methods In order to study the detoxification mechanisms of heavy metals by microorganisms in this typical rare earth mining area, the study area was divided into three parts (mining area, residential area and control area). Analysis of microbial community diversity, structure and functional abundance using high-throughput sequencing techniques. Analysis of the effect of heavy metal pollution on the abundance of heavy metal resistance genes in soils of different regions using real-time fluorescence quantitative PCR. Results The results showed that the heavy metal pollution rules: mining area > residential area > control area. Under the condition of long-term heavy metal pollution, the original microbial community composition was changed, and the species richness and evenness of soil in mining areas were higher than that in residential areas. The high-throughput sequencing analysis showed that existed metal-resistant microbial communities such as Actinobacteria, Proteobacteria, Korarchaeota and so on under the stress of heavy metal. High concentrations of heavy metals can inhibit the activities of catalase and sucrase. According to Tax4Fun function prediction analysis, heavy metal accumulation increased the ABC transporter protein in microbial function. The results of fluorescence quantification experiments also demonstrated that the abundance of heavy metal resistance genes, czcA, czcB, czcC and czcD, encoding ABC transporter proteins, increased with increasing heavy metal concentrations. Conclusions In conclusion, the accumulation of heavy metals not only changed the soil physicochemical properties and the microbial community structure, but also decreased soil enzyme activities and increased the abundance of resistance genes, which activated the detoxification mechanism of heavy metals. which provided a reference for future ecological remediation.


2018 ◽  
Vol 28 (1) ◽  
pp. 41 ◽  
Author(s):  
Riza Zainuddin Ahmad

The agriculture land including farmland is decreasing caused by conversion to industrial area and settlement, therefore post-mining area is used to be farmlands. The utilization of post-mining area causes serious health problem in animals and humans due to heavy metal waste pollution (Ag, As, Cd, Co, Cr, Cu, Hg, Ni, and Pb). Efforts to overcome the pollution of heavy metals on farms can be carried out by preventing and controlling waste in post-mining and industry areas. There are several mycoremediation methods to recover heavy metal polluted land such as biosorption, bioaccumulation, bioprecipitation, bioreduction, and bioleaching. Mycoremediation is a process to remove pollutants or heavy metals from soil using potential fungi (<em>Aspergillus flavus, Penicillium </em>sp<em>, Fusarium </em>sp<em>, Saccharomyces cerevisiae</em>). Mycoremediation is chosen due to economical cost, efficient, and environmentally friendly.


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