scholarly journals Pengaruh Pemberian Streptozotocin Dosis Tunggal terhadap Kadar Glukosa Tikus Wistar (Rattus norvegicus)

2021 ◽  
Vol 9 (1) ◽  
pp. 62-67
Author(s):  
Nihayah Enis Munjiati
Keyword(s):  
Single Dose ◽  
Blood Glucose ◽  
Glucose Level ◽  
Rattus Norvegicus ◽  
Pancreatic Beta Cells ◽  
Wistar Rats ◽  
Control Group ◽  
Control Group Design ◽  
Glucose Levels ◽  
Before And After

Diabetes Mellitus (DM) is a disease caused by metabolic disorders in the pancreas, which is characterized by an increased glucose levels or hyperglycemia due to decreased insulin levels from the pancreas. In animal research, DM is often caused by administration of diabetogenic agents. Streptozotocin works by forming free radicals that can damage the pancreatic beta cells; therefore, insulin production can be disrupted. This study aims to determine the glucose level on Wistar Rats (Rattus norvegicus) before and after a single dose streptozotocin-induced. This study was conducted experimentally with a posttest-interpretation design with a control group design. The result demonstrated that the average blood glucose level before the induction of streptozotocin was 92,63 (SD 10,487) mg/dL, after being induced by streptozotocin was 235,56 (SD 164,929) mg/dL with a difference of 142,938 (SD 160,543) mg/dL. In this study, it can be concluded that the induction of a single dose of streptozotocin has an effect on increasing blood glucose levels on Wistar Rats (Rattus norvegicus).

scholarly journals PENGARUH PEMBERIAN EKSTRAK BUNGA DAN DAUN PEPAYA (Carica papaya L.) TERHADAP KADAR GLUKOSA DARAH TIKUS WISTAR (Rattus norvegicus L.) YANG HIPERGLIKEMIK

2017 ◽  
Vol 17 (2) ◽  
pp. 143
Author(s):  
Frendy Grenaldy Tangkumahat ◽  
Johnly A Rorong ◽  
Feti Ftimah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Control Group ◽  
Glucose Levels ◽  
Flower Extract

PENGARUH PEMBERIAN EKSTRAK BUNGA DAN DAUN PEPAYA (Carica papaya L.) TERHADAP KADAR GLUKOSA DARAH TIKUS WISTAR                                  (Rattus norvegicus L.) YANG HIPERGLIKEMIKABSTRAKTelah dilakukan penelitian tentang Pengaruh Pemberian Ekstrak Bunga dan Daun Pepaya (Carica papaya L.) terhadap Kadar Glukosa Darah Tikus Wistar (Rattus norvegicus L.) yang hiperglikemik. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak bunga dan daun pepaya terhadap kadar glukosa darah tikus wistar. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan 30 ekor tikus wistar jantan yang dibagi dalam 5 kelompok perlakuan yaitu kelompok kontrol positif (K+) diberi Air, kelompok kontrol negatif (K-) diberi Sukrosa, Ekstrak bunga pepaya dosis 150 mg/kg BB (B1), Ekstrak bunga pepaya dosis 260 mg/kg BB (B2), Ekstrak daun pepaya dosis 100 mg/kg BB (D1) dan Ekstrak daun pepaya dosis 170 mg/kg BB (D2). Data diperoleh dari pemeriksaan kadar glukosa darah puasa setelah tikus diinduksi aloksan secara intraperitional dan setelah masa adaptasi selama 7 hari dan masa perlakuan selama 3 hari setelah diinduksi sediaan per oral (p.o.). Pemberian ekstrak Ethanol bunga dan daun pepaya (Carica Papaya L.) berpengaruh terhadap penurunan kadar glukosa darah tikus wistar (Ratus Norvegicus L.) yang Hiperglikemik. Dosis ekstrak bunga dan daun pepaya yang efektif dalam menurunkan kadar glukosa darah tikus yang hiperglikemia akibat diinduksi dengan aloksan adalah 260 mg/Kg BB dan dosis ekstrak daun 170 mg/Kg BB atau setara dengan bunga dan daun pepaya basah 200 g.Kata Kunci : Carica papaya  L, Kadar Glukosa Darah, Aloksan, Hiperglikemik. EFFECT OF FLOWER AND  PAPAYA LEAF EXTRACT (Carica papaya L.)  ON WISTAR RATS BLOOD GLUCOSE LEVEL (Rattus norvegicus L.) WHICH HYPERGLYCEMIC ABSTRACTA Research had been carried out to determine the Effect of Flower and Papaya Leaf Extract (Carica papaya L.) on Wistar Rats Blood Glucose Level (Rattus norvegicus L.) which hyperglycemic. The purpose of this research is to know the effect of flower and papaya leaf extract on wistar rats blood glucose level.   This research used  glucose tolerance test method. Used 30 male wistar rats divided into 5 groups of treatment that is positive control group (K+) given Water, negative control group (K-) given Sucrose, papaya flower extract dose 150 mg/kg BW (B1), papaya flower extract dose 260 mg/kg BW (B2), papaya leaf extract dose 100 mg/kg BW (D1) and papaya leaf extract dose 170 mg/kg BW (D2). Data were obtained from fasting blood glucose examination after rats induced alloxan intraperitional and after a 7-day adaptation period and a 3-day treatment period after induced oral preparation (p.o.). The giving of Ethanol flower extract and papaya leaf (Carica Papaya L.) had an effect on the decrease of blood glucose level of Wistar rats (Ratus norvegicus L.) which Hyperglycemic. The dose of flower and papaya leaf extract is effective in lowering blood glucose levels of hyperglycemic rats induced by alloxan is dose of papaya extract 260 mg/Kg BW and dose of leaf extract 170 mg/kg BW or equal of 200g  wet papaya flowers and leaves.Keywords: Carica Papaya L, Blood Glucose Levels, Alloxan, Hyperglycemic.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Uji efek ekstrak daun papaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Cynthia C.C. Senduk ◽  
Henoch Awaloei ◽  
Edward Nangoy
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Control Group ◽  
Elevated Blood ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sugar Levels

Abstract: Indonesia is a tropical country with more than 9,600 kinds of medicinal plants; one of them is papaya. Extract of papaya leaf (Carica papaya) is presumed to have hypoglycemia effect because it contains flavonoid, alkaloid, saponin, and tannin. This study aimed to evaluate the effect of papaya leaf extract on elevated blood glucose levels on Wistar rats induced with alloxan. This was an experimental study with 18 male Wistar rats as subjects, divided into 6 groups (3 rats in each group). Group 1, the negative control group, was given aquadest only. Group 2, the positive control group, was treated with alloxan 120 mg/kg body weight (BW) followed by novomix 0.2 iu/200 g BW. Group 3 and 4 were treated with alloxan 120 mg/kg BW followed by papaya leaf extract dosing 250 mg and 500 mg/kg BW respectively. Group 5 and 6 were treated with papaya leaf extract dosing 250 mg and 500 mg/kg BW without alloxan induction. Blood glucose levels were measured on day 1, day 2, and day 3 every six hours at 0, 6, 12, 18, and 24 hours. The results showed that 250 mg/kg BW and 500 mg/kg BW of papaya leaf extract could reduce the elevated blood glucose on Wistar rats for 12 hours after treatment. Conclusion: The extract of papaya leaves could reduce blood sugar levels in hyperglicemic Wistar rats induced by alloxan. Keywords: papaya leaves (carica papaya L.), blood sugar levels, alloxan. Abstrak: Indonesia merupakan salah satu negara beriklim tropis yang memiliki banyak jenis tumbuhan. Sekitar 9.600 spesies tumbuhan merupakan tumbuhan yang berkhasiat obat, salah satunya ialah pepaya. Ekstrak daun pepaya (Carica papaya L.) diduga mempunyai efek hipoglikemia karena mengandung flavonoid, alkaloid, saponin, dan tannin. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak daun pepaya (Carica papaya L.) terhadap kadar gula darah tikus Wistar (Rattus norvegicus) yang diinduksi aloksan. Jenis penelitian ini eksperimental. Subyek penelitian yang digunakan ialah 18 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok (setiap kelompok terdiri dari 3 tikus). Kelompok 1 (K1) merupakan kelompok kontrol negatif hanya diberikan aquades; kelompok 2 (K2) merupakan kelompok kontrol positif diberikan aloksan dan novomix 0,2 iu/200 g BB; kelompok 3 (K3) dan kelompok 4 (K4) merupakan kelompok perlakuan diberikan aloksan kemudian ekstrak daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB; kelompok 5 (K5) dan kelompok 6 (K6) merupakan kelompok perlakuan diberikan daun pepaya dengan dosis 250 mg/kg BB dan 500 mg/kg BB tanpa induksi aloksan. Data diperoleh dari pemeriksaan kadar gula darah dari semua kelompok tikus Wistar pada hari ke-1, ke-2, dan ke-3 pada jam ke-0, 6, 12, 18, dan 24. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun pepaya dengan dosis 250 mg dan 500 mg/kg BB tikus berefek menurunkan kadar gula darah tikus wistar selama 12 jam pasca pemberian ekstrak daun pepaya. Simpulan: Ekstrak daun pepaya berpotensi memiliki efek dalam menurunkan kadar gula darah pada tikus Wistar.Kata kunci: daun pepaya (carica papaya L.), kadar gula darah, aloksan

scholarly journals The effect of daun sendok (Plantago major) extract on the blood glucose level of Balb/C mice induced by streptozotocin

2010 ◽  
Vol 8 (2) ◽  
pp. 66-71
Author(s):  
PUTRI SATRIANY ◽  
R. P. ANDRI PUTRANTO ◽  
IPOP SYARIFAH
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Simple Random Sampling ◽  
Control Group ◽  
Plantago Major ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels

Satriany P, Putranto RPA, Syarifah I. 2010. The effect of daun sendok (Plantago major) extract on the blood glucose level of Balb/C mice induced by streptozotocin. Biofarmasi 8: 66-71. This research aimed to determine the effect of daun sendok (Plantago major L.) extract on the blood glucose level of Balb/C mice induced by streptozotocin. This study was a laboratory experimental pre and post-test control group design. The subjects used were 16 male mice. Then, mice were induced with streptozotocin by a dose of 65 mg/kg body weight intraperitoneally freshly dissolved in 0.02 M citrate saline buffer. Mice used were mice with blood glucose levels â‰¥200 mg/dL. Furthermore, mice were divided into 2 groups by a simple random sampling. Group 1 was given metformin in a dose of 1.3 mg/mice/day and group 2 was given daun sendok extract in a dose of 1000 mg/kg body weight/day. At the 2nd week, it was measured for the glucose levels from the blood of mice tail by a Blood Glucose Stick Meter (Gluco DrTM). The data obtained were statistically analyzed by independent samples t-test by using SPSS Programme for Microsoft Windows release 17.0. The significance was set at p<0.05. The difference of average blood glucose levels pre vs post experiment, metformin group was -145.87 mg/dL and daun sendok group was -85.00 mg/dL (p=0.024). The experiment results showed that daun sendok extract in a dose of 1000 mg/kg body weight/day could reduce the blood glucose levels in Balb/C mice induced by streptozotocin.

scholarly journals Pemberian resveratrol oral mencegah peningkatan F2-Isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang dipapar tartrazine

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Yulyani . ◽  
Wimpie Pangkahila ◽  
Ferbian M. Siswanto
Keyword(s):  
Oral Administration ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Before And After ◽  
Male Wistar Rats ◽  
Immuno Assay ◽  
Enzyme Immuno Assay

Abstract: This study was aimed to prove that oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus). This was an experimental study using the pretest-posttest control group design. Subjects were 24 rats (Rattus norvegicus), Wistar strain, healthy, 2-3 months old, weighing 200-220 g, divided into 2 groups with 12 rats each. The first group (P0), the control group, was given a placebo of 2 ml distilled water 2 hours prior to the administration of tartrazine 75 mg/kg body weight. The second group (P1), the treatment group, was given resveratrol of 20 mg/kg 2 hour prior to the administration of tartrazine 75 mg/kg. Rats’ urine was collected before and after treatment for 4 weeks. Level of F2-isoprostane was examined by using an 8-iso-PGF2α enzyme immuno assay kit. The comparative analysis of the pretest groups showed that there was no difference between the average levels of F2-isoprostane in both groups (5.45±0.62 ng/mL in P0 group vs 5.42±0.64 ng/mL in P1 group) (P > 0.05). Meanwhile, after treatment for 4 weeks, the average level of F2-isoprostane in the P0 group was significantly different from the P1 group (6.61±0.93 ng/mL vs 3.79±0.48 ng/mL) (P < 0.01). Analysis of the treatment effect showed a significant increase of F2-isoprostane level in the P0 group, and a significant decrease in the P1 group (P < 0.01). Conclusion: Oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus).Keywords: tartrazine, resveratrol, F2-isoprostaneAbstrak: Tujuan penelitian untuk membuktikan pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan dalam urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine. Jenis penelitian eksperimental dengan menggunakan pretest-posttest control group design. Subyek penelitian ialah 24 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 2-3 bulan, dengan berat badan 200-220 gr, dibagi menjadi 2 kelompok, masing-masing berjumlah 12 ekor tikus. Kelompok pertama (P0) ialah kelompok kontrol, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan plasebo berupa aquadest 2 ml. Kelompok kedua (P1) ialah kelompok perlakuan, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan resveratrol 20 mg/kg BB. Saat sebelum dan sesudah perlakuan selama 4 minggu, urin tikus dikoleksi untuk pemeriksaan kadar F2-isoprostan menggunakan 8-iso-PGF2α enzyme immuno assay kit. Analisis komparasi sebelum perlakuan (pretest) menunjukkan rerata kadar F2-isoprostan pada kedua kelompok tidak berbeda nyata (5,45±0,62 ng/mL vs 5,42±0,64 ng/mL) (P > 0,05). Setelah perlakuan selama 4 minggu, rerata kadar F2-isoprostan pada kelompok P0 berbeda nyata dibandingkan kelompok P1 (6,61±0,93 ng/mL vs 3,79±0,48 ng/mL) (P < 0,01). Analisis efek perlakuan menunjukkan terjadi peningkatan kadar F2-isoprostan pada kelompok P0 dan penurunan bermakna pada kelompok P1 (P < 0,01). Simpulan: Pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine.Kata kunci: tartrazine, resveratrol, F2-isoprostan, urin

scholarly journals Pemberian ekstrak air buah sawo (Manilkara zapota L.) menurunkan kadar glukosa darah tikus (rattus norvegicus) diabetes mellitus

2016 ◽  
Vol 3 (3) ◽  
pp. 180
Author(s):  
Effatul Afifah
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels

<p><strong>ABSTRACT</strong><br /><br /><em><strong>Background</strong>: Diabetes mellitus (DM) is a chronic metabolic problem disorder characterized by hyperglicemia which is caused by insulin deficiency produced by β-pancreas cells, thus causing abnormalities of carbohydrate, protein, and fat metabolism, and tend to cause complications. </em></p><p><em><strong>Objectives</strong>: To know the effect of sapodilla extract water on blood glucose level of diabetic induce mice. </em></p><p><em><strong>Methods</strong>: This was an experimental study with pre-post control group design. Sapodilla extract water (EABS) was fed to group of mice with alloxan diabetes induction. Twenty four DM induced mice were separated into 4 groups, e.g. control without and with medication of glibenclamide, EABS 1 (treated with 3.6 mL/200 g body weight), and EABS 2 (treated with 7.2 mL/200 g body weight). Mice were then measured for their blood glucose level at the day of 3, 14, and 30. </em></p><p><em><strong>Results</strong>: EABS 1 and EABS 2 decreased blood glucose levels at week of 1st, 2nd, and 3rd after induction. The greatest reduction was shown by EABS 2 at the 3rd week. EABS decreased blood glucose level of mice induced DM and signifi cantly shown at glibenclamide group, followed by EABS 2 and EABS 1.</em></p><p><em><strong>Conclusions</strong>: EABS reduced blood glucose levels of diabetic mice and signifi cantly shown for glibenclamide group, followed by EABS 2 and EABS 1.</em></p><p><strong>KEYWORDS</strong>:<em> diabetes mellitus, sapodilla extract water, blood glucose</em></p><p><br /><strong>ABSTRAK</strong></p><p><em><strong>Latar belakang</strong>: Diabetes mellitus (DM) merupakan penyakit gangguan metabolik kronis yang ditandai dengan hiperglikemia yang disebabkan oleh kekurangan insulin yang dihasilkan oleh sel β-pankreas sehingga menimbulkan kelainan metabolisme karbohidrat, protein dan lemak, dan cenderung menimbulkan komplikasi.</em></p><p><em><strong>Tujuan</strong>: Mengetahui efek pemberian ekstrak buah air sawo terhadap kadar glukosa darah tikus yang diinduksi DM.</em></p><p><em><strong>Metode</strong>: Penelitian ini merupakan penelitian eksperimental murni dengan rancangan pre-post test control group design dengan memberikan intervensi ekstrak buah air sawo (EABS) pada kelompok tikus yang diberikan induksi DM menggunakan aloksan. Sebanyak 24 tikus yang diinduksi DM dikelompokkan ke dalam 4 perlakuan, antara lain kontrol tanpa dan dengan pengobatan glienclamide, EABS 1 (diberi 3,6 mL/200 g berat badan), dan EABS 2 (7,2 mL/200 g berat badan). Tikus diukur kadar gula darahnya pada hari ke-3, 14, dan 30. </em></p><p><em><strong>Hasil</strong>: EABS mampu menurunkan kadar glukosa darah pada tikus, baik pada kelompok EABS 1 maupun EABS 2 pada minggu ke-1, ke-2, dan ke-3 setelah induksi. Hasil penurunan kadar glukosa yang paling besar terjadi pada kelompok EABS 2 pada minggu ke-3 perlakuan. EABS dapat menurunkan kadar glukosa darah tikus yang diinduksi DM dan paling signifi kan terjadi pada kelompok glibenclamide diikuti oleh EABS 2 dan EABS 1.</em></p><p><em><strong>Kesimpulan</strong>: EABS mampu menurunkan kadar glukosa darah pada tikus yang diinduksi alloxan.</em></p><p><strong>KATA KUNCI</strong><em>: diabetes mellitus, ekstrak air buah sawo, glukosa darah</em></p>

scholarly journals Uji efek ekstrak daun sirih hutan (Piper aduncum L.) terhadap kadar gula darah pada tikus wistar (Rattus novergicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Silvia R.H. Sitinjak ◽  
Jane Wuisan ◽  
Christi Mambo
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Betel Leaf ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Decrease Blood Glucose ◽  
Piper Aduncum

Abstract: Forest betel leaf (Piper aduncum L.) has been known bypublicto have efficacy in wound healing, stop vomiting, reducing nausea, aiding digestion, as an antiseptic, as well as killing bacteria, fungi and viruses. Dewi et al. found that 50 mg/kgBW and 100 mg/kgBW red betel leaf extract caused a decrease blood glucose levels in Wistar rats (Rattus norvegicus) induced by alloxan. This study was aimed to identify the effect of forest betel leaf extract on blood glucose levels on Wistar rats induced by alloxan. Subjects were Wistar rats with total of 15 samples divided into five groups: a negative control group and four hyperglycemic groups induced by 130 mg/kgBW of alloxan. Forest betel leaf extract was administered with dose of 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW on each groups of hyperglycemic rats, and group0.4 IU/100grBW of novomix injection was administered on the positive control group. Blood glucose levels were measured in the 0, 30, 60, 90, and 120 minutes on day one, two, and three. The results showed that 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW of forest betel leaf extract had a tendency to decrease blood glucose levels of Wistar rats induced by alloxan.Keywords: piper aduncum L., forest betel leaf, blood glucose levels, alloxan Abstrak: Daun sirih hutan (Piper aduncum L.) telah dikenal oleh masyarakat dan mempunyai khasiat dalam penyembuhan luka, menghentikan muntah, mengurangi mual, melancarkan pencernaan, sebagai antiseptik, membunuh bakteri dan jamur serta virus. Menurut penelitian Dewi dkk, pemberian ekstrak daun sirih merah dengan dosis 50 mg/kgBB dan 100 mg/kgBB menyebabkan penurunan kadar gula darah pada tikus Wistar (Rattus norvegicus) yang diinduksi dengan aloksan. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun sirih hutan terhadap kadar gula darah pada tikus Wistar yang diinduksi dengan aloksan. Subjek penelitian berupa tikus Wistar berjumlah 15 ekor yang dibagi dalam 5 kelompok terdiri atas kelompok kontrol negatif dan 4 kelompok tikus Wistar yang diberi aloksan dengan dosis 130 mg/kgBB tikus yang menyebabkan tikus Wistar dalam keadaan hiperglikemik. Tikus hiperglikemik diberi ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB tikus, dan 100 mg/kgBB tikus, dan yang terakhir kelompok kontrol positif diberi suntikan novomix 0,4 IU/100grBB tikus. Data diperoleh dari pemeriksaan kadar gula darah pada semua kelompok pada hari pertama, kedua, dan ketiga pada menit ke-0, 30, 60, 90, dan 120. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB dan 100 mg/kgBB tikus memiliki kecenderungan untuk menurunkan kadar gula darah tikus Wistar yang diinduksi oleh aloksan. Kata kunci: piper aduncumL., daun sirih hutan, kadar gula darah, aloksan

scholarly journals Uji efek air perasan albedo semangka kuning (Citrilus lanatus (Thunb.) Matsum. & Nakai) terhadap kadar glukosa darah pada tikus wistar (Rattus norvegicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Savitri Muhammad Nur ◽  
Henoch Awaloei ◽  
Jane Wuisan
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
White Layer ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract: Indonesian has various natural resources, second as largest after Brazil. One of the traditional medicine that has been used is watermelon. The part of watermelon that is known to be used as medicine is its white layer between the peel and the fruit, albedo. This study aimed to determine the effect of yellow watermelon albedo juice on blood glucose levels in wistar rats induced by alloxan. Subjects consisted of 18 male Wistar rats divided into 6 groups as follows: a negative control group, 3 groups of hyperglycemic rats induced with alloxan 120 mg/kg body weight; and 2 groups given only yellow watermelon albedo juice. Hyperglycemic rats were given yellow watermelon albedo with dose of 9 g/kg body weight and 4,5 g/kg body weight; positive control group was given novomix; and two other groups were given only yellow watermelon albedo juice. Blood glucose levels of each rat were evaluated on day 1, day 2, and day 3 before the treatment after 6 hours, 12 hours, 18 hours, 24 hours. The results showed that administration of yellow watermelon albedo juice 9 g/kg body weight of Wistar rats reduced the blood glucose level until 126.67 mg/dl, meanwhile the dose 4.5 g/kg body weight reduced the blood glucose level until 173.67 mg/dl. Conclusion: Administration of yellow watermelon albedo juice of 9 g/kg body weight and 4.5 g/kg body weight could reduce blood glucose levels of hyperglycemic Wistar rats induced by alloxan. Keywords: yellow watermelon albedo, hyperglycemia, blood glucose level Abstrak: Indonesia merupakan negara yang memiliki keanekaragaman hayati terkaya kedua didunia setelah Brazil. Salah satu obat tradisional yang digunakan ialah buah semangka. Bagian dari semangka yang digunakan ialah lapisan yang berwarna putih yakni antara kulit dan daging buah semangka disebut albedo. Penelitian ini bertujuan untuk menguji pengaruh pemberian air perasan albedo semangka kuning terhadap kadar glukosa darah pada tikus wistar yang diinduksi aloksan. Subjek penelitian yaitu 18 ekor tikus Wistar jantan yang dibagi menjadi 6 kelompok, yaitu: kelompok kontrol negatif; 3 kelompok tikus Wistar hiperglikemik yang diinduksi aloksan dengan dosis 120 mg/kg BB; dan 2 kelompok tikus Wistar yang hanya diberikan albedo semangka kuning. Kelompok tikus yang hiperglikemik diberi albedo semangka kuning dengan dosis 9 g/kg BB dan 4,5 g/kg BB; kelompok kontrol positif diberikan novomix; dan 2 kelompok hanya diberikan albedo semangka kuning. Pemeriksaan kadar glukosa darah dari semua kelompok tikus Wistar dilakukan pada hari ke-1, hari ke-2, dan hari ke-3 pada jam ke 0, 6, 12, 18, dan 24. Hasil penelitian memperlihatkan pemberian air perasan albedo semangka kuning dosis 9 g/kg BB dapat menurunkan kadar glukosa darah sebanyak 126,67 mg/dl sedangkan dosis 4,5 g/kg BB sebanyak 173,67 mg/dl. Simpulan: Air perasan albedo semangka kuning dengan dosis 9 g/kg BB dan 4,5 g/kg BB dapat menurunkan kadar glukosa darah tikus Wistar hiperglikemik yang diinduksi aloksan.Kata kunci: albedo semangka kuning, hipeglikemi, kadar glukosa darah

scholarly journals Influence of Nutrition Education with Calendar Method in Diabetic Patients' Blood Glucose

2018 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
Setyoadi Setyoadi ◽  
Heri Kristianto ◽  
Siti Nur Afifah
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Nutrition Education ◽  
Community Health Center ◽  
Control Group ◽  
P Value ◽  
Diabetic Patients ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Before And After

Diabetes mellitus is a disease that required good self-management. Noncompliance in diet and meal plans cause the instability of blood glucose levels. Nutrition education calendar method can improve knowledge and ability to consume food that matches the number, hours and types with dietary adjustments listed in the calendar diet. This study aimed to determine the effect of nutrition education calendar method on blood glucose levels of patients with type 2 diabetes mellitus in Community Health Center Pakis Malang. Pre-experimental design one group pretest-posttest with purposive sampling was conducted in this study and sample obtained as many as 21 people. Blood glucose levels were measured before and after the nutrition education calendar methods. Compliance in using calendar method with the observation sheet. Statistical analysis values obtained by Wilcoxon, the p-value of 0.007 (p <0.05). The results of the analysis, 16 respondents showed a decrease in blood glucose levels and 5 respondents experienced an increase in blood glucose levels after the given intervention. It can be concluded that there are differences between blood glucose levels before and after nutrition education calendar method. Differences in blood glucose levels can be influenced by controlling diet respondent in accordance with the calendar method in education, but also antidiabetic drugs, and sports. Should be added to the control group to determine objectively the effect of nutrition education on the calendar method.

scholarly journals UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 160
Author(s):  
Adinda Fransisca Pongoh ◽  
Edwin De Queljoe ◽  
Henki Rotinsulu
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Control Group ◽  
Homogeneity Test ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan.  ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.)  dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

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