Faculty Opinions recommendation of Nucleolin associates with a subset of the human Ro ribonucleoprotein complexes.

Ro ribonucleoprotein particles (Ro RNPs) are complexes of several proteins with a small RNA polymerase III-transcribed Ro RNA. Despite their relative abundance and evolutionary conservation no function has as yet been ascribed to these complexes. Also their subcellular distribution is still largely unknown as immunofluorescence studies concerning their localization have produced conflicting data. We have used cell enucleation to fractionate cells into cytoplasmic and nuclear fractions. Analysis of these fractions revealed an exclusively cytoplasmic localization for the Ro RNPs. The majority of the Ro RNAs are shown to be stably associated with all three known Ro RNP proteins. Although no Ro RNAs could be detected in the nuclear fraction, the Ro RNP-specific proteins were abundantly present. These nuclear non-Ro RNA-associated proteins are shown to be capable of binding Ro RNAs.

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Analysis of protein-RNA interactions within Ro ribonucleoprotein complexes

Nucleic Acids Research ◽

10.1093/nar/19.19.5173 ◽

1991 ◽

Vol 19 (19) ◽

pp. 5173-5180 ◽

Cited By ~ 68

Author(s):

Ger J.M. Pruijn ◽

Rob L. SIobbe ◽

Walther J.van Venrooij

Keyword(s):

Ribonucleoprotein Complexes ◽

Ro Ribonucleoprotein

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DEVELOPMENT OF APPROACHES FOR DETECTION OF GENOME-INTEGRATED PROVIRAL DNA OF THE HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) IN ULTRA LOW CONCENTRATIONS USING THE CRISPR/CAS SYSTEM

Molecular Diagnostics and Biosafety – 2020. Russian national scientific and practical conference with international participation (October, 6–8, 2020): Conference Proceedings ◽

10.36233/978-5-9900432-9-9-118 ◽

2020 ◽

Author(s):

M.A. Tyumentseva ◽

◽

A.I. Tyumentsev ◽

V.G. Akimkin ◽

◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Health Monitoring ◽

Biological Samples ◽

Proviral Dna ◽

Guide Rnas ◽

Ribonucleoprotein Complexes ◽

Low Concentrations ◽

Immunodeficiency Virus ◽

Hiv 1

For the effective functioning of supervisory and health monitoring services, it is necessary to introduce modern molecular technologies into their practice. Therefore, the task of developing new effective methods for detecting pathogen, for example HIV, based on CRISPR/CAS genome editing systems, remains urgent. In the present work, guide RNAs and specific oligonucleotides were developed for preliminary amplification of highly conserved regions of the HIV-1 genome. The developed guide RNAs make it possible to detect single copies of HIV-1 proviral DNA in vitro as part of CRISPR/CAS ribonucleoprotein complexes in biological samples after preliminary amplification.

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ASSESSMENT OF EFFICIENCY AND OFF-TARGET ACTIVITY OF CRISPR/CAS RIBONUCLEOPROTEIN COMPLEXES

Molecular Diagnostics and Biosafety – 2020. Russian national scientific and practical conference with international participation (October, 6–8, 2020): Conference Proceedings ◽

10.36233/978-5-9900432-9-9-98 ◽

2020 ◽

Author(s):

Y.V. Mikhaylova ◽

◽

M.A. Tyumentseva ◽

A.A. Shelenkov ◽

Y.G. Yanushevich ◽

...

Keyword(s):

High Sensitivity ◽

Correct Choice ◽

Target Sequence ◽

Dna Breaks ◽

Gene Encoding ◽

Guide Rna ◽

Guide Rnas ◽

Ribonucleoprotein Complexes ◽

Chemokine Receptor Ccr5 ◽

Target Activity

In this study, we assessed the efficiency and off-target activity of the CRISPR/CAS complex with one of the selected guide RNAs using the CIRCLE-seq technology. The gene encoding the human chemokine receptor CCR5 was used as a target sequence for genome editing. The results of this experiment indicate the correct choice of the guide RNA and efficient work of the CRISPR- CAS ribonucleoprotein complex used. CIRCLE-seq technology has shown high sensitivity compared to bioinformatic methods for predicting off-target activity of CRISPR/CAS complexes. We plan to evaluate the efficiency and off-target activity of CRISPR/CAS ribonucleoprotein complexes with other guide RNAs by slightly adjusting the CIRCLE-seq-technology protocol in order to reduce nonspecific DNA breaks and increase the number of reliable reads.

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The role of CPEB family proteins in the nervous system function in the norm and pathology

Cell & Bioscience ◽

10.1186/s13578-021-00577-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Eugene Kozlov ◽

Yulii V. Shidlovskii ◽

Rudolf Gilmutdinov ◽

Paul Schedl ◽

Mariya Zhukova

Keyword(s):

Nervous System ◽

Gene Regulation ◽

Neural Development ◽

Fragile X ◽

Autism Spectrum ◽

Mrna Transport ◽

Ribonucleoprotein Complexes ◽

Posttranscriptional Gene Regulation ◽

Nervous System Function ◽

Functional Features

AbstractPosttranscriptional gene regulation includes mRNA transport, localization, translation, and regulation of mRNA stability. CPEB (cytoplasmic polyadenylation element binding) family proteins bind to specific sites within the 3′-untranslated region and mediate poly- and deadenylation of transcripts, activating or repressing protein synthesis. As part of ribonucleoprotein complexes, the CPEB proteins participate in mRNA transport and localization to different sub-cellular compartments. The CPEB proteins are evolutionarily conserved and have similar functions in vertebrates and invertebrates. In the nervous system, the CPEB proteins are involved in cell division, neural development, learning, and memory. Here we consider the functional features of these proteins in the nervous system of phylogenetically distant organisms: Drosophila, a well-studied model, and mammals. Disruption of the CPEB proteins functioning is associated with various pathologies, such as autism spectrum disorder and brain cancer. At the same time, CPEB gene regulation can provide for a recovery of the brain function in patients with fragile X syndrome and Huntington's disease, making the CPEB genes promising targets for gene therapy.

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The presence of protein kinase activity and acceptors of phosphate groups in nonpolysomal cytoplasmic messenger ribonucleoprotein complexes of embryonic chicken muscle.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)50730-4 ◽

1979 ◽

Vol 254 (9) ◽

pp. 3137-3140

Author(s):

J Bag ◽

B H Sells

Keyword(s):

Protein Kinase ◽

Kinase Activity ◽

Protein Kinase Activity ◽

Ribonucleoprotein Complexes ◽

Embryonic Chicken ◽

Chicken Muscle ◽

Messenger Ribonucleoprotein ◽

Phosphate Groups

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The noncoding MIR100HG RNA enhances the autocrine function of transforming growth factor β signaling

Oncogene ◽

10.1038/s41388-021-01803-8 ◽

2021 ◽

Author(s):

Panagiotis Papoutsoglou ◽

Dorival Mendes Rodrigues-Junior ◽

Anita Morén ◽

Andrew Bergman ◽

Fredrik Pontén ◽

...

Keyword(s):

Growth Factor ◽

Target Genes ◽

Rna Binding ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Tgfβ Signaling ◽

Ribonucleoprotein Complexes ◽

Expression Of Genes ◽

Downstream Effectors ◽

Human Carcinomas

AbstractActivation of the transforming growth factor β (TGFβ) pathway modulates the expression of genes involved in cell growth arrest, motility, and embryogenesis. An expression screen for long noncoding RNAs indicated that TGFβ induced mir-100-let-7a-2-mir-125b-1 cluster host gene (MIR100HG) expression in diverse cancer types, thus confirming an earlier demonstration of TGFβ-mediated transcriptional induction of MIR100HG in pancreatic adenocarcinoma. MIR100HG depletion attenuated TGFβ signaling, expression of TGFβ-target genes, and TGFβ-mediated cell cycle arrest. Moreover, MIR100HG silencing inhibited both normal and cancer cell motility and enhanced the cytotoxicity of cytostatic drugs. MIR100HG overexpression had an inverse impact on TGFβ signaling responses. Screening for downstream effectors of MIR100HG identified the ligand TGFβ1. MIR100HG and TGFB1 mRNA formed ribonucleoprotein complexes with the RNA-binding protein HuR, promoting TGFβ1 cytokine secretion. In addition, TGFβ regulated let-7a-2–3p, miR-125b-5p, and miR-125b-1–3p expression, all encoded by MIR100HG intron-3. Certain intron-3 miRNAs may be involved in TGFβ/SMAD-mediated responses (let-7a-2–3p) and others (miR-100, miR-125b) in resistance to cytotoxic drugs mediated by MIR100HG. In support of a model whereby TGFβ induces MIR100HG, which then enhances TGFβ1 secretion, analysis of human carcinomas showed that MIR100HG expression correlated with expression of TGFB1 and its downstream extracellular target TGFBI. Thus, MIR100HG controls the magnitude of TGFβ signaling via TGFβ1 autoinduction and secretion in carcinomas.

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