Faculty Opinions recommendation of The nitration of tau protein in neurone-like PC12 cells.

Induction by nerve growth factor of neurite outgrowth in PC12 cells is transcription-dependent and is associated with the accumulation of tau protein. It was recently shown that short-term treatment with staurosporine, a protein kinase alkaloid inhibitor, induced an elevation of tau protein levels and outgrowth of stable neurites. In this study, we analyzed the mechanism(s) by which nerve growth factor and staurosporine exert their effects on tau levels. We demonstrate that nerve growth factor affects tau mRNA stability, thus contributing to the observed increase in tau mRNA levels. On the other hand, tau mRNA levels were not affected by the treatment with staurosporine. We also demonstrate that the phosphorylation of tau protein was reduced after treatment of PC12 cells with nerve growth factor or staurosporine, as shown by immunoblot analysis using specific antibodies and alkaline phosphatase treatment. Thus, regulation of tau levels by nerve growth factor appears to be mediated by transcriptional, post-transcriptional and posttranslational steps, whereas the effect of staurosporine on tau levels may be attributed to its effect on the state of phosphorylation of the protein.

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Bioassay-Guided Isolation of Neuroprotective Compounds fromUncaria rhynchophyllaagainst Beta-Amyloid-Induced Neurotoxicity

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/802625 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 30

Author(s):

Yan-Fang Xian ◽

Zhi-Xiu Lin ◽

Qing-Qiu Mao ◽

Zhen Hu ◽

Ming Zhao ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Intracellular Calcium ◽

Pc12 Cells ◽

Tau Protein ◽

Beta Amyloid ◽

Active Ingredients ◽

Uncaria Rhynchophylla ◽

Model Of Alzheimer’S Disease ◽

Calcium Overloading

Uncaria rhynchophyllais a component herb of many Chinese herbal formulae for the treatment of neurodegenerative diseases. Previous study in our laboratory has demonstrated that an ethanol extract ofUncaria rhynchophyllaameliorated cognitive deficits in a mouse model of Alzheimer’s disease induced by D-galactose. However, the active ingredients ofUncaria rhynchophyllaresponsible for the anti-Alzheimer’s disease activity have not been identified. This study aims to identify the active ingredients ofUncaria rhynchophyllaby a bioassay-guided fractionation approach and explore the acting mechanism of these active ingredients by using a well-established cellular model of Alzheimer’s disease, beta-amyloid- (Aβ-) induced neurotoxicity in PC12 cells. The results showed that six alkaloids, namely, corynoxine, corynoxine B, corynoxeine, isorhynchophylline, isocorynoxeine, and rhynchophylline were isolated from the extract ofUncaria rhynchophylla. Among them, rhynchophylline and isorhynchophylline significantly decreased Aβ-induced cell death, intracellular calcium overloading, and tau protein hyperphosphorylation in PC12 cells. These results suggest that rhynchophylline and isorhynchophylline are the major active ingredients responsible for the protective action ofUncaria rhynchophyllaagainst Aβ-induced neuronal toxicity, and their neuroprotective effect may be mediated, at least in part, by inhibiting intracellular calcium overloading and tau protein hyperphosphorylation.

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Neuroprotective effects of Wnt/β-catenin signaling pathway against Aβ -induced tau protein over-phosphorylation in PC12 cells

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2016.01.130 ◽

2016 ◽

Vol 471 (4) ◽

pp. 628-632 ◽

Cited By ~ 6

Author(s):

Jin Wang ◽

Ying Jing ◽

Lili Song ◽

Ying Xing

Keyword(s):

Signaling Pathway ◽

Pc12 Cells ◽

Tau Protein ◽

Neuroprotective Effects

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Tau Protein Is Hyperphosphorylated in a Site-Specific Manner in Apoptotic Neuronal PC12 Cells

Journal of Neurochemistry ◽

10.1046/j.1471-4159.2000.0752346.x ◽

2008 ◽

Vol 75 (6) ◽

pp. 2346-2357 ◽

Cited By ~ 34

Author(s):

Jianwen Zhang ◽

Gail V. W. Johnson

Keyword(s):

Pc12 Cells ◽

Tau Protein ◽

Site Specific ◽

Specific Manner ◽

Neuronal Pc12 Cells ◽

A Site

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Interaction of single and multi wall carbon nanotubes with the biological systems: tau protein and PC12 cells as targets

Scientific Reports ◽

10.1038/srep26508 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 67

Author(s):

Hojjat Alizadeh Zeinabad ◽

Alireza Zarrabian ◽

Ali Akbar Saboury ◽

Ali Mohammad Alizadeh ◽

Mojtaba Falahati

Keyword(s):

Surface Tension ◽

Cell Death ◽

Carbon Nanotube ◽

Cell Line ◽

Pc12 Cells ◽

Conformational Changes ◽

Tau Protein ◽

Structural Changes ◽

Multiwall Carbon Nanotube ◽

Multi Wall Carbon Nanotubes

Abstract Subtle changes in the structure of nanoparticles influence their surface tension and corresponding interaction with cells and proteins. Here, the interaction of the single wall carbon nanotube (SWCNT) and multiwall carbon nanotube (MWCNT) with different surface tension with tau protein was evaluated using a variety of techniques including far and near circular dichroism, fluorescence spectroscopy, dynamic light scattering, Zeta potential, and TEM evaluation. Also the cytotoxicity of SWCNT and MWCNT on the PC12 cell line as a model of nervous system cell line was investigated by the MTT, LDH, acridine orange/ethidium bromide staining, flow cytometry, caspase 3 activity, cell and membrane potential assays. It was observed that SWCNT induced more structural changes of tau protein relative to MWCNT/tau protein interaction. It was also revealed that SWCNT and MWCNT impaired the viability and complexity of PC12 cells in different modes of cytotoxicity. Analysis of cellular outcomes indicated that MWCNT in comparison with SWCNT resulted in induction of necrotic modes of cell death, whereas apoptotic modes of cell death were activated in SWCNT-incubated cells. Together these findings suggest that surface tension may be used to determine how nanoparticle structure affects neurotoxicity and protein conformational changes.

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