scholarly journals The role of beluntas (pluchea indica less.) leaf extract in preventing the occurrence of fibroblasts hyperproliferation: An in vitro preliminary study

2019 ◽  
Author(s):  
Sakti Charlia Maharani ◽  
Indah Julianto ◽  
Suci Widhiati
Keyword(s):  
Leaf Extract ◽  
In Vitro Study ◽  
Collagen I ◽  
Control Group ◽  
Antibody Treatment ◽  
Average Decrease ◽  
Matrix Deposition ◽  
Post Hoc

Beluntas (Pluchea indica Less.) is a herbal plant which contains variety of benefits. Quercetin, one of flavonoid, is the most bioactive agent in beluntas leaf. Collagen inhibition by quercetin may modulate extracellular matrix deposition and inhibit the formation of hypertrophic scar. This was an in vitro study with senescent fibroblasts to determine the role of beluntas leaf extract in preventing the occurence of fibroblasts hyperproliferations. There were 4 groups were stained by anti-collagen I antibodies and secondary antibody. Flowcytometry analysis was done to measure the fibroblasts density. Anova test was performed with a value of p=0.000 (p<0.05). A post hoc analysis showed significant differences in the average decrease of fibroblasts that absorbs staining anti-collagen I antibody treatment group compared with the control group. There were significant effects of beluntas leaf extract in preventing the occurrence of fibroblasts hyperproliferations. Beluntas leaf extract with a concentration of 80 mol/L had the most significant effect on the fibroblasts density. Thus beluntas leaf extract has the ability in preventing the occurrence of fibroblasts hyperproliferation.

scholarly journals The Role of Ferrous Ion in the Effect of the Gadolinium-Based Contrast Agents (GBCA) on the Purkinje Cells Arborization: An In Vitro Study

Diagnostics ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2310
Author(s):  
Achmad Adhipatria Perayabangsa Kartamihardja ◽  
Winda Ariyani ◽  
Hirofumi Hanaoka ◽  
Ayako Taketomi-Takahashi ◽  
Noriyuki Koibuchi ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Contrast Agents ◽  
Purkinje Cells ◽  
In Vitro Study ◽  
Control Group ◽  
Control Groups ◽  
Post Hoc ◽  
The Brain

Gadolinium deposition in the brain has been observed in areas rich in iron, such as the dentate nucleus of the cerebellum. We investigated the role of Fe2+ in the effect of gadolinium-based contrast agents (GBCA) on thyroid hormone-mediated Purkinje cell dendritogenesis in a cerebellar primary culture. The study comprises the control group, Fe2+ group, GBCA groups (gadopentetate group or gadobutrol group), and GBCA+Fe2+ groups. Immunocytochemistry was performed with an anti-calbindin-28K (anti-CaBP28k) antibody, and the nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI). The number of Purkinje cells and their arborization were evaluated with an analysis of variance with a post-hoc test. The number of Purkinje cells was similar to the control groups among all treated groups. There were no significant differences in dendrite arborization between the Fe2+ group and the control groups. The dendrite arborization was augmented in the gadopentetate and the gadobutrol groups when compared to the control group (p < 0.01, respectively). Fe2+ significantly increased the effect of gadopentetate on dendrite arborization (p < 0.01) but did not increase the effect of gadobutrol. These findings suggested that the chelate thermodynamic stability and Fe2+ may play important roles in attenuating the effect of GBCAs on the thyroid hormone-mediated dendritogenesis of Purkinje cells in in vitro settings.

scholarly journals Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Philipp Körner ◽  
Luca Georgis ◽  
Daniel B. Wiedemeier ◽  
Thomas Attin ◽  
Florian J. Wegehaupt
Keyword(s):  
Gastroesophageal Reflux ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Tooth Wear ◽  
Control Group ◽  
Erosive Tooth Wear ◽  
Custom Made ◽  
Post Hoc ◽  
Bovine Enamel

Abstract Background This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. Methods Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1–5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2–5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. Results The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). Conclusions After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

scholarly journals Effect of Incorporation of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone: An in vitro Study

2017 ◽  
Vol 7 (3) ◽  
pp. 86-91
Author(s):  
Anubhuti Bakshi ◽  
Rajat Dang
Keyword(s):  
Sodium Hypochlorite ◽  
Testing Machine ◽  
Surface Hardness ◽  
In Vitro Study ◽  
Dimensional Accuracy ◽  
Control Group ◽  
Time Interval ◽  
Type Iv ◽  
Post Hoc

ABSTRACT Aim To evaluate surface hardness and dimensional accuracy of type IV dental stone after incorporation of disinfectants. Materials and methods A total of 100 specimens, 50 (40 mm⨯ 10 mm) disk shaped and 50 frustum cone shaped, were fab- ricated from type IV dental stone after incorporation of various disinfectants, namely glutaraldehyde (2%), iodophor (1.76%), chlorhexidine (2%), and sodium hypochlorite (1%), for evaluation of surface hardness and dimensional accuracy. Surface hardness was tested after 1- and 24-hour time interval using Rockwell hard- ness testing machine. Dimensional accuracy was measured using traveling microscope. Data obtained were compiled and analyzed statistically by using analysis of variance and post hoc test. Results Results showed significant decrease in surface hard- ness of samples incorporated with disinfectants at 1 hour as compared with control (distilled water). Surface hardness of all the samples increased with time and was almost equal to control group at 24-hour interval, with iodophor showing the maximum value in comparison with the other disinfectants used. When dimensional accuracy of all the groups was compared, iodophor and sodium hypochlorite were found to cause negative alterations. Conclusion Within the limitations of this in vitro study, it was concluded that incorporation of disinfectants in die stone during mixing did not affect the hardness value at 24-hour interval. Dimensional accuracy was altered by iodophor and sodium hypochlorite, but remained unaffected by chlorhexidine and glutaraldehyde. How to cite this article Bakshi A, Dang R. Effect of Incorpo- ration of Disinfectants on Surface Hardness and Dimensional Accuracy of Die Stone An in vitro Study. Int J Prosthodont Restor Dent 2017;7(3):86-91.

scholarly journals Dissolution capacity of orange and eucalyptus oils versus endodontic types of cement (in vitro study)

2022 ◽  
Vol 10 (19) ◽  
pp. 29-33
Author(s):  
Sebastián M. Ulloa-Alvarez ◽  
María E. Guerrero-Coello ◽  
José E. Torracchi-Carrasco
Keyword(s):  
In Vitro Study ◽  
Control Group ◽  
Filling Material ◽  
Cement Sample ◽  
Orange Oil ◽  
Eucalyptus Oil ◽  
Before And After ◽  
The Difference ◽  
Post Hoc

Introduction: Endodontic retreatment seeks reentry to the root canal system to remove all filling material, identification and correction of pathological or iatrogenic defects. Objective: This study aimed to compare the dissolving capacity of eucalyptus oil, orange oil, and distilled water versus three types of endodontic cements. Materials and methods: Two hundred and four stainless steel molds were fabricated, where each cement sample was placed to the edge and left to set in the incubator, simulating normal oral conditions for 7 days. Subsequently, the solubility of each cement was analysed by immersing the samples in eucalyptus and orange oil for ten minutes in a static and ultrasonic environment. The solubility of the cement was evidenced by the difference between the weight before and after contact with the oils, data were statistically analysed with the ANOVA test and the post hoc Tukey bootstrap means test with 1,000 repetitions. Results: The control group and eucalyptus oil having no significant effect (p>0.05), the ultrasonic environment was found to be effective (p<0.05), whereas orange oil had better dissolution effects (p=0.004). Conclusions: The most susceptible cement was Sealapex, while the least soluble was MTA-Fillapex in all the solvents studied.

Comparative evaluation of shear bond strength of composite to dentin in presence of different dental liners: An in-vitro study

2021 ◽  
Author(s):  
Ladan Ranjbar Omrani ◽  
Saba Tohidkhah ◽  
Elham Ahmadi ◽  
Mahdi Abbasi ◽  
Reza Morvaridi Farimani
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Control Group ◽  
The One ◽  
Post Hoc

Abstract Background: The aim of the current study was to evaluate and compare the influence of Dycal, Lime-lite, Theracal LC, Biodentine, Resin-modified glass ionomer cement (RMGIC), and Activa Bioactive as the pulp capping material on the shear bond strength of resin composite to dentin.Methods: A total of 70 extracted caries-free molars were randomly assigned to seven groups. Six test groups were covered with various protective liners: Dycal (GD), Theracal LC (GT), lime-lite (GL), Activa Bioactive (GA), Biodentine (GB), RMGIC (GR). The control group (GC)received no liner pretreatment. Each sample was bonded to resin composite using the total-etch tetric N bond adhesive. The samples were then tested for shear bond strength using the universal testing machine at a cross-head speed of 1 mm/min until bond failure occurred. The data were analyzed using the one-way ANOVA test followed by the Tamhane post-hoc test for pairwise comparisons of the groupsResults: Independent of the type of the applied liner, all groups exhibited inferior SBS to dentine compared to the control group. GT and GR showed significantly higher shear bond strength than GB and GD, which showed the lowest shear bond strength. GL and GA also had significantly lower SBS results than GT. The mode of fracture was predominantly cohesive in GD, GB, and GT and adhesive in GA.Conclusion: This present study concludes that the bond strength of resin-composite to dentine can be affected differently using various types of liners.

scholarly journals In vitro study of potential antibacterial pometia pinnata leaf extract in Neisseria gonorrhoeae

MEDISAINS ◽  
2021 ◽  
Vol 19 (3) ◽  
pp. 72
Author(s):  
Gandi Ari Savitri Widayani ◽  
Muhammad Choiroel Anwar ◽  
Sudirman Sudirman
Keyword(s):  
Antibiotic Resistance ◽  
Neisseria Gonorrhoeae ◽  
Leaf Extract ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Leaf Extracts ◽  
Extract Concentration

Background: Gonorrhea cases experiencing antibiotic resistance are increasing due to inadequate treatment or failure to contribute to an increase in antibiotic resistance cases; WHO suggests adding herbal therapy treatment methods is expected to minimize the occurrence of drug resistance as much as possible. Previous studies that have been carried out using other herbal plants have not shown any potential to inhibit the growth of Neisseria gonorrhoeae bacteria, seen from the amount of extract concentration and the dosage and content of the compounds in the contents monitoring the time.Objective: To analyze the difference in the effect of various concentrations of Matoa Leafs Extract (Pometia Pinnata) compared to 500mg Levofloxacin on the increase in the growth inhibition of Neisseria gonorrhoeae.Methods: This is true-experimental research with a post-test-only control group with a randomized design. The sample in this study was the microorganism Neisseria gonorrhoeae obtained through vaginal swabs in 2 females (FSW) positive for Gonorrhea by gram staining and microscopic examination and culture on Chocolate Agar Plant (CAP) media. The culture was then suspended in CAP media. Matoa Leaf extract concentration of 100%, 80%, 60%, 40%, 20%, positive control with Levofloxacin 500 mg and negative control with distilled water with four replications and monitoring in 24 hours, 48 hours, 72 hours, and 96 hours. Data analysis used Kruskal-Wallis, Mann-Whitney, and cohen's test. Result: Levofloxacin 500mg in inhibiting Neisseria Gonorrhoeae bacteria is very effective, and Matoa Leaf extracts 60% and 40% P=0,026 have a strong enough potential to inhibit the growth of Neisseria gonorrhoeae bacteria with the same potential strength. The cohen's test 1.4 effect the levofloxacin 500mg provides a significant influence inhibiting Neisseria Gonorrhoeae.Conclusion: Matoa Leaf extract 60%, and 40% have solid antibacterial potential, although not as strong as Levofloxacin 500mg.

scholarly journals Comparison of Flow and Solubility Properties of Mineral Trioxide Aggregate Cement with Two Additive Plasticizing Agents: An In Vitro Study

2021 ◽  
Vol 1 (3) ◽  
Author(s):  
Ricardo Novak Savioli
Keyword(s):  
Physical Properties ◽  
Analysis Of Variance ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Control Group ◽  
Significance Level ◽  
Sodium Sulfonate ◽  
Tukey Test ◽  
Post Hoc

Proposition: To evaluate the physical properties of flow and solubility of pure and improved mineral trioxide aggregate (MTA) cement with the addition of two plasticizing agents: sodium ether polycarboxylate and sodium naphthalene sulfonate at three different concentrations. Material and Methods: Flow and solubility tests were carried out using the established methodology recommended by the 57 ANSI/ADA and ISO 6876:2012 standards. Pure MTA was used as control group and was compared to the MTA containing sodium ether polycarboxylate and sodium naphthalene sulfonate at concentrations of 0.5%, 1.0% and 1.5%. Data were analyzed by one-way analysis of variance followed by a post-hoc Tukey test at a 5% significance level. Results: Sodium ether polycarboxylate increased the MTA flow at the three concentrations. The flow increased with increasing concentration (P<0.05). Sodium sulfonate naphthalene also facilitated flow, though it was inferior to sodium ether polycarboxylate. Sodium naphthalene sulfonate promoted a significant increase in the solubility of MTA (p=0.000). Conclusions: The additives improved the physical properties of MTA. Sodium ether polycarboxylate was found to be superior to sodium naphthalene sulfonate at all concentrations.

scholarly journals Fracture Resistance of Retreated Roots Using Different Retreatment Systems

2011 ◽  
Vol 05 (04) ◽  
pp. 387-379 ◽  
Author(s):  
Kursat Er ◽  
Tamer Tasdemir ◽  
Seyda Herguner Siso ◽  
Davut Celik ◽  
Sabri Cora
Keyword(s):  
Fracture Resistance ◽  
In Vitro Study ◽  
Control Group ◽  
Root Fracture ◽  
Vertical Root Fracture ◽  
Root Canals ◽  
Gutta Percha ◽  
Ah Plus ◽  
Post Hoc

ABSTRACTObjectives: This study was designed to evaluate the fracture resistance of retreated roots using different rotary retreatment systems. Methods: Forty eight freshly extracted human canine teeth with single straight root canals were instrumented sequentially increasing from size 30 to a size 55 using K-files whit a stepback technique. The teeth were randomly divided into three experimental and one control groups of 12 specimens each. The root canals were filled using cold lateral compaction of gutta-percha and AH Plus (Dentsply Detrey, Konstanz, Germany) sealer in experimental groups. Removal of gutta-percha was performed with the following devices and techniques: ProTaper Universal (Dentsply Maillefer, Ballaigues, Switzerland), R-Endo (Micro-Mega, Besançon, France), and Mtwo (Sweden & Martina, Padova, Italy) rotary retreatment systems. Control group specimens were only instrumented, not filled or retreated. The specimens were then mounted in copper rings, were filled with a self-curing polymethylmethacrylate resin, and the force required to cause vertical root fracture was measured using a universal testing device. The force of fracture of the roots was recorded and the results in the various groups were compared. Statistical analysis was accomplished by one-way ANOVA and a post hoc Tukey tests. Results: There were statistically significant differences between the control and experimental groups (P<.05). However, there were no significant differences among the experimental groups. Conclusions: Based on the results, all rotary retreatment techniques used in this in vitro study produced similar root weakness. (Eur J Dent 2011;5:387-392)

Comparative Evaluation of Shear Bond Strength of Composite to Dentin in Presence of Calcium Releasing Liners: An In-Vitro Study

2021 ◽  
Author(s):  
Saba Tohidkhah ◽  
Elham Ahmadi ◽  
Mahdi Abbasi ◽  
Reza Morvaridi Farimani ◽  
Ladan Ranjbar Omrani
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Control Group ◽  
The One ◽  
Post Hoc

Abstract Background: The aim of current study was to evaluate and compare the influence of Dycal, Lime-lite, Theracal LC, Biodentine, Resin-modified glass ionomer cement (RMGIC), and Activa Bioactive as the pulp capping material on the shear bond strength of resin composite to dentin.Methods: A total of 70 extracted caries-free molars were randomly assigned to seven groups. Six test groups were covered with various protective liners: Dycal (GD), Theracal LC (GT), lime-lite (GL), Activa Bioactive (GA), Biodentine (GB), RMGIC (GR). The control group (GC)received no liner pretreatment. Each sample was bonded to resin composite using the total-etch tetric N bond adhesive. The samples were then tested for shear bond strength using the universal testing machine at a cross-head speed of 1 mm/min until bond failure occurred. The data were analyzed using the one-way ANOVA test followed by the Tamhane post-hoc test for pairwise comparisons of the groupsResults: Independent of the type of the applied liner, all groups exhibited inferior SBS to dentine compared to the control group. GT and GR showed significantly higher shear bond strength than GB and GD, which showed the lowest shear bond strength. GL and GA also had significantly lower SBS results than GT. The mode of fracture was predominantly cohesive in GD, GB, and GT and adhesive in GA.Conclusion: This present study concludes that the bond strength of resin-composite to dentine can be affected differently using various types of liners.

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