Protein linking fatty acids and its genetic regulation in children having food allergy

2022 ◽  
pp. 46-49
Author(s):  
N. G. Prikhodchenko ◽  
T. A. Shumatova ◽  
S. V. Voronin ◽  
D. V. Kovalenko
Keyword(s):  
Fatty Acids ◽  
Blood Serum ◽  
Biological Fluids ◽  
Genetic Regulation ◽  
Control Sample ◽  
Food Allergies ◽  
Elisa Method ◽  
Diagnostic Significance ◽  
Pcr Method ◽  
Fabp Gene

Objective: Study the effect of the Ala54Thr FABP gene polymorphism on the produce of the intestinal FABP fraction in blood serum. urine and coprofiltrate in children having food allergies.Methods: The content of the FABP intestinal fraction in urine, feces, and blood serum was determined using ELISA method. The study of FABP genes polymorphism (G163A, Ala54Thr) was carried out using PCR method.Results: Statistically significant increase of the FABP level in blood serum, urine and feces in children with FA was detected in various biological fluids. The distribution of FABP2 alleles and genotypes obeyed the Hardy-Weinberg law (χ 2 = 0; p = 1,000) and did not significantly differ from the distribution of genotypes in children having FA andin the control sample (p = 0.638).Conclusions: The study did not reveal an association of the pathological genotype FABP G163A, (Ala54Thr) with the hyperproduction of the FABP intestinal fraction in children having FA, confirming the diagnostic significance of this marker increase during exacerbation of the disease.

scholarly journals Analysis of the content of I-FABP in urine and blood serum in children with gastrointestinal food allergy

2021 ◽  
pp. 66-71
Author(s):  
N. G. Prikhodchenko ◽  
T. A. Shumatova ◽  
A. Nee ◽  
E. S. Zernova
Keyword(s):  
Food Allergy ◽  
Blood Serum ◽  
Clinical Symptoms ◽  
Biological Fluids ◽  
Allergic Sensitization ◽  
Control Group ◽  
Healthy Children ◽  
Diagnostic Significance ◽  
Fatty Acid Binding ◽  
Gastrointestinal Food Allergy

Abstract Introduction. Protein-induced enteropathy is one of the common manifestations of gastrointestinal food allergy in young children. The search of non-invasive methods for intestine estimation is especially relevant for early diagnosis and timely prevention of exacerbation.The aim of the study was to determine the serum and urinal levels of the intestinal fraction of the protein binding fatty acids in children with protein-induced enteropathy and to evaluate their clinical and diagnostic significance.Materials and methods. It was examined 36 children with protein-induced enteropathy aged 1.5 months to 1 year and 20 healthy children (control group). All patients underwent esophagogastroenteroscopy with morphological examination of biopsy specimens for differential diagnosis with congenital gastrointestinal diseases. The Cow’s Milk-related Symptom Score (CoMMiS) was used to evaluate clinical symptoms. The intestinal fraction of a fatty acid binding protein was determined for all children in blood serum, urine by enzyme- linked immunosorbent analysis.Results. It was found the increase its serum level (125.20 ± 23.79 pg / ml), and urinal level (0.164 ± 0.031 pkg / ml) compared with the control group (19.21 ± 4.94 pg / ml, 00.039 ± 4.62 pkg / ml, respectively, p < 0.05). There were found direct strong correlations between the severity of gastrointestinal manifestations and its serum and urine level (p < 0.05).Discussion. Epithelial barrier damage provides an increased penetration of intact food allergens. It plays a key role in allergic sensitization, and it is the first pathogenetic link of allergic inflammation in most cases. A statistically significant I-FABP increased level in all studied biological fluids correlates with morphological changes in the children with protein-induced enteropathy, as well as with clinical symptoms of gastrointestinal tract lesions. That confirms its high informational value for an indirect assessment of the state of the intestinal barrier.Conclusions. Thus, an increase in serum and urinal I-FABP levels in children with protein-induced enteropathy confirms the clinical and diagnostic significance of determining this biomarker in all biological fluids. Its high sensitivity and specificity of determination in urine are promising for use in pediatric practice.

Inhibition of rat blood serum N-acetyl-L-tyrosine ethylesterase by fatty acids

1969 ◽  
Vol 34 (5) ◽  
pp. 1470-1474 ◽  
Author(s):  
E. Simonianová ◽  
M. Petáková ◽  
M. Rybák
Keyword(s):  
Fatty Acids ◽  
Blood Serum ◽  
Rat Blood

scholarly journals SEMIMICROMETHOD FOR UNSATURATED FATTY ACIDS OF BLOOD SERUM

1953 ◽  
Vol 202 (1) ◽  
pp. 417-423
Author(s):  
Hilda F. Wiese ◽  
Arild E. Hansen
Keyword(s):  
Fatty Acids ◽  
Blood Serum ◽  
Unsaturated Fatty Acids

scholarly journals Up-regulation of the expression of the gene for liver fatty acid-binding protein by long-chain fatty acids

1996 ◽  
Vol 319 (2) ◽  
pp. 483-487 ◽  
Author(s):  
Claire MEUNIER-DURMORT ◽  
Hélène POIRIER ◽  
Isabelle NIOT ◽  
Claude FOREST ◽  
Philippe BESNARD
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Fold Increase ◽  
Long Chain Fatty Acids ◽  
Liver Fatty Acid ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Fabp Gene ◽  
Long Chain

The role of fatty acids in the expression of the gene for liver fatty acid-binding protein (L-FABP) was investigated in the well-differentiated FAO rat hepatoma cell line. Cells were maintained in serum-free medium containing 40 µM BSA/320 µM oleate. Western blot analysis showed that oleate triggered an approx. 4-fold increase in the cytosolic L-FABP level in 16 h. Oleate specifically stimulated L-FABP mRNA in time-dependent and dose-dependent manners with a maximum 7-fold increase at 16 h in FAO cells. Preincubation of FAO cells with cycloheximide prevented the oleate-mediated induction of L-FABP mRNA, showing that protein synthesis was required for the action of fatty acids. Run-on transcription assays demonstrated that the control of L-FABP gene expression by oleate was, at least in part, transcriptional. Palmitic acid, oleic acid, linoleic acid, linolenic acid and arachidonic acid were similarly potent whereas octanoic acid was inefficient. This regulation was also found in normal hepatocytes. Therefore long-chain fatty acids are strong inducers of L-FABP gene expression. FAO cells constitute a useful tool for studying the underlying mechanism of fatty acid action.

Technology of biscuits with a balanced fatty acid composition

2021 ◽  
Vol 30 (11) ◽  
pp. 34-39
Author(s):  
G.V. Posnova ◽  
◽  
N.G. Ivanova ◽  
I.A. Nikitin ◽  
G.A. Shinov ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Healthy Lifestyle ◽  
Raw Materials ◽  
Control Sample ◽  
School Age Children ◽  
Plant Raw Materials ◽  
Degree Of Satisfaction

The article presents the results of research on the development of the technology of biscuits using non-traditional plant raw materials. In the course of the work, the replacement of a part of the premium wheat flour in the dairy crust recipe with a mixture of rice (17%) and sesame (11%) and the replacement of butter with camelina butter in an amount of 5% by weight of the product was justified. It was found that the introduction of functional raw materials gives the products a moderately sweet milk taste with a pleasant nutty aftertaste and orange color. The resulting products are characterized by a balanced fatty acid composition of ω-3 and ω-6, enriched with minerals and vitamins. Calculation of the nutritional value showed that the consumption of 100 g of the developed biscuits covers the daily need of an adult for polyunsaturated fatty acids of the ω-3 and ω-6 families by 37–75% and 19,4–31,5, respectively. The protein content rises by 1,5 times, and the carbohydrate content decreases by 1,16 times. At the same time, the degree of satisfaction of the daily requirement for potassium increases – by 2,3%, in calcium – by 7–7,5%, in phosphorus – by 12,6%, in magnesium – by 16,3%, in iron – by 7,3–13,3%. The degree of satisfaction of the daily requirement for vitamins B1 also increases – by 19,7–20,9%, B2 – by 2,2–2,3%, PP – by 8,1–9,3%, E – by 1,6–1,7% compared to the control sample of dairy cakes. The developed products can be recommended for preventive nutrition, people adhering to a healthy lifestyle, as well as school-age children, pregnant and lactating women who have a deficiency of ω-3 and ω-6 fatty acids in the diet

PREVALENCE OF PNEUMOCYSTIS JIROVECII IN FREQUENTLY ILL CHILDREN ON ADMISSION TO THE RESPIRATORY DEPARTMENT AND DURING SANATORIUM REHABILITATION

2021 ◽  
Vol 100 (2) ◽  
pp. 104-111
Author(s):  
L.V. Feklisova ◽  
◽  
N.V. Karazhas ◽  
M.K. Khadisova ◽  
E.I. Likhanskaya ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Active Form ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Pharyngeal Wall ◽  
Blood Neutrophils ◽  
Pneumocystis Infection ◽  
Chain Fatty Acids

The aim of the study was to determine the frequency of occurrence and to assess the significance of markers of pneumocystosis in frequently ill children (FIC) during inpatient treatment and during rehabilitation in a sanatorium. Materials and methods of research: 115 children with recurrent respiratory diseases were observed: 52 were treated in a hospital and 63 underwent rehabilitation in a sanatorium. Biological samples (oropharyngeal swabs, saliva, feces and blood serum) were examined for pneumocystosis. Laboratory techniques used: a polymerase chain reaction (PCR) to detect DNA pathogens, an indirect immunofluorescence reaction (NRIF) – antigens, and immunoforment analysis (IFA) – specific antibodies of immunoglobulins (Ig) of classes M (IgM) and G (IgG). The material for laboratory research was smears from the posterior pharyngeal wall (PCR, NRIF) and blood serum (IFA). Microbiological studies included seeding, isolation of cultures with quantitative counting of colony-forming units in samples of oropharyngeal swabs, feces. Determination of the functional activity of the oropharynx microbiocenosis by the concentration of short-chain fatty acids in saliva by means of GLC method. Phagocytic activity of blood neutrophils was also investigated. Results: in hospital patients, markers for pneumocystis were found in 60% of cases, including the active form (40%) and pneumocystis (13%); in the sanatorium – 33% of cases, with active infection (26%) and detection of pneumocystis (17%). Inpatients were diagnosed with clinical variants of pneumocystis infection: pneumonia, obstructive bronchitis, acute respiratory infections; schoolchildren have been diagnosed with active pneumocystis infection and carriage. Dysbiotic disorders of the loci of the oropharynx and intestines were identified in all examined subjects, inhibition of the phagocytic activity of blood neutrophils and shifts in the concentration of short-chain fatty acids have been observed. Conclusion: a high proportion of active pneumocystis infection (40% and 27% of cases) was revealed in FIC treated in the hospital and in the sanatorium, which represents a risk group for infection/reinfection with pneumocystosis in the premises.

scholarly journals Microalgae based innovative animal fat and proteins replacers for application in functional baked products

2018 ◽  
Vol 3 (1) ◽  
pp. 427-436 ◽  
Author(s):  
Maria-Styliani-Georgia Kafyra ◽  
Sofia Papadaki ◽  
Marios Chronis ◽  
Magdalini Krokida
Keyword(s):  
Fatty Acids ◽  
Raw Material ◽  
Food Allergies ◽  
Bakery Products ◽  
Omega 3 ◽  
Microalgal Oil ◽  
Animal Fat ◽  
Organoleptic Characteristics ◽  
Freeze Dried ◽  
Baked Products

Abstract Animal fat and proteins, such as milk butter and eggs, are the main ingredients of baked products, and are frequently blamed for food allergies, obesity, cancer and type II diabetes. Therefore, there is an urgent need to replace these ingredients with healthier ones without degrading the organoleptic characteristics of the final product. Microalgae are a great source of protein, minerals and lipids such as omega-3 and omega-6 fatty acids, which are beneficial for human health, offering multiple health benefits such as antioxidant and anti-aging activity. In this study, Chlorella vulgaris microalgae were the selected raw material for the innovative replacers because of their high content in proteins and polyunsaturated fatty acids. The obtained microalgal oil was colour corrected and used to produce brioche-type baked products with 100% animal fat substitution. For protein recovery, the aquatic extract was freeze-dried, producing a dry protein powder that fully substituted the animal protein in the baked products. Finally, the development of bakery products with 100% replacement of both animal fat and protein was achieved. These innovative bakery products showed equal performance to the commercial ones, and even improved organoleptic characteristics according to the sensorial analysis that occurred.

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