scholarly journals EFFECT OF GRAPHENE OXIDE ON REGENERATED PLANTS OF WHITE POPLAR × ASPEN HYBRID

2021 ◽  
Vol 11 (2) ◽  
pp. 35-47
Author(s):  
Nataliya Strekalova ◽  
Olga Zakharova ◽  
Petr Baranchikov ◽  
Alexander Gusev

In this work, we have studied the effect of graphene oxide on the regenerants of the white poplar × aspen hybrid when they were transferred to non-sterile greenhouse conditions during clonal micropropagation. It was shown that a suspension of the nanomaterial at a concentration of 1.5 μg / L stimulated the growth of plant height in the hybrid (white poplar × aspen), but also increased the number of wilted leaves. An increase in the concentration of nanomaterial to 3 μg / L caused a negative effect on seedlings. The number of surviving shoots decreased by 17% relative to the control, plant height decreased by 25%. A decrease in the number of leaves and adapted microclones was also observed: 15% of plants adapted against 55% in the control group. The general condition of the seedlings of this group was assessed only by 3 points out of 5. Analysis of binaccumulation of graphene oxide in plant organs has not showed pronounced foci of nanomaterial accumulation. Thus, graphene oxide had a multidirectional effect on the development of seedlings of the hybrid (white poplar × aspen). The results of the study can be used in assessing the environmental safety of graphene-like materials in relation to plants, as well as for improving the biotechnology of clonal micropropagation of forest plantations

2021 ◽  
Vol 11 (2) ◽  
pp. 48-58
Author(s):  
Nataliya Strekalova ◽  
Olga Zakharova ◽  
Petr Baranchikov ◽  
Alexander Gusev

In this work, we studied the effect of graphene oxide on regenerants of downy birch when transferred to non-sterile greenhouse conditions (adaptation stage) during clonal micropropagation. During the experiment, it was found that a solution of graphene oxide at a concentration of 1.5 μg / l had a beneficial effect on the development of leaves and plant adaptability (+ 8%). However, with an increase in the concentration of nanomaterial to 3 μg / L, the survival rate decreased by 10% , the plant height - by 25%, and the number of adapted plants - by 35% relative to the control group. In addition, general condition of the seedlings of this group was assessed only by 3 points on a 5-point scale. The study of the content of graphene oxide in plant organs did not reveal pronounced foci of accumulation, which may indicate an indirect effect of nanomaterial on plants through changes in environmental conditions, for example, the availability of root nutrition or the composition of microbiota in the root zone. The mechanisms of ecological and biological impact of carbon nanomaterials on plants are not fully known and require further in-depth research


2019 ◽  
Vol 11 (17) ◽  
pp. 227 ◽  
Author(s):  
L. J. Damasceno ◽  
V. F. A. Silva ◽  
J. N. da Silva ◽  
P. A. Silva ◽  
C. C. Lima ◽  
...  

The reduction in the leaf area is one of the causes in the fall in soybean (Glycine max) productivity as it depends on the production of photoassimilates generated by the leaves, so any factor that interferes in its leaf area may affect the production. The attack of defoliating insects is among such factors. They cause a marked drop in grain yield due to its direct action, therefore, reducing the leaf area, consequently reducing the photosynthetic rate of the plant. The agronomic characteristics of the cultivars may interfere on the level of tolerance of the plant to this type of stress. The objective of this study was to evaluate the influence of defoliation levels on the vegetative and reproductive stages on the development and yield of grains in soybean cultivars. The experimental design was in randomized blocks, in a 2×11×2 factorial scheme, with four replicates. Factors consisted of defoliation stage (vegetative and reproductive), treatment levels (T1-control plant and ten treatments of artificial defoliation) and soybean cultivars (BRS 9090 RR and BRS 8890 RR). The following variables were evaluated: grain yield, dry mass of the pod, leaf dry mass, stem and root dry mass, plant height, stem diameter, number of leaves per plant, length and width of roots. It was observed that the defoliation had a negative effect on the productivity components of the cultivars, with the highest decrease in the reproductive stage, except for the treatment R5, 100% defoliation at the R5 stage, which was also reduced. In relation to the cultivars, the BRS 8890 RR was 27% better in grain yield in relation to BRS 9090 RR.


2018 ◽  
Vol 2 (1) ◽  
pp. 1-10
Author(s):  
Yuanita Rachmawati ◽  
Budi Setiadi Daryono ◽  
Ganies Riza Ariestya

Yogyakarta Agroecosystem has 158,600 ha of critical land spread over three zones. Two areas are Karst Land, located on Agroecosystem II includes Gunungsewu Hills, Gunungkidul and III covers Dlingo Bantul Hills and Sentolo Hills Kulon Progo Regency.. Karst Land is certainly provides stress to plants. These research purposes are examining the phenotype character of superior melon Cultivar TACAPA compare to parents and offsprings phenotypes. The phenotype characters are based on plant height, leaf number, time of melon flowering, water content of plants, and fruit and seed productivity. This experiment was done by Split Plot Design with Completely Randomized Design (CRD) with 4 kinds of treatment (control plant media, Gunungsewu, Dlingo, and Sentolo), 7 experimental units cultivars: TACAPA, TA, TP, PT, AT, Action 434, PI 371795), and 4 replications. Research result reveals that most of the phenotypic characters including plant height, number of leaves, fruit weight, and number of seeds produced have relatively no significant effect between treatment and control, while the phenotypic first time flowering time and water content of the plant, have a noticeable difference.


2019 ◽  
pp. 1-8
Author(s):  
C. E. Igwe ◽  
J. N. Azorji ◽  
M. O. Nwachukwu

Experiments were carried out in the Nursery of the Department of Forestry and Environmental Management, the Michael Okpara University of Agriculture, Umudike in Abia State during 2016 and 2017 cropping seasons to determine  the effect of NPK fertilizer on the growth performance of three selected crops, Zea mays, Abelmoschus esculentus and Vigna unguiculata grown on a derelict kaolin mined soil. The experiment comprised of treatment pots which has three rates of fertilizer on the kaolin soil each (kaolin soil +NPK (40 kg), kaolin soil + NPK (30 kg) and kaolin soil + NPK (20 kg) kaolin soil with zero treatment (Control 2) and a control 1(un-mined) soil, it was replicated three in Complete Randomized Design (CRD). Parameters assessed include Plant height (cm) and the number of leaves weekly for six (6) weeks. Data were analysed using ANOVA at p< 0.05. The result obtained showed that growth performance of the test crops was positively affected by NPK treatment based on the quantity applied with the highest recorded height (16.50) and the number of the leaf (11.27) in Kaolin mined soil combined with 40 kg of fertilizer when compared with the control. Plant height obtained with various fertilizer treatments were in the order: 16.50>15.99>14.65 and 11.27>10.20>9.01 for the number of leaves. The result of the macro element levels in the plants showed that the nutrient uptake and accumulation were enhanced by both NPK fertilizer and the quantity added with the highest level of nutrient on the plants obtained in Kaolin soil combined with 40kg of NPK fertilizer. The study showed that kaolin mined degraded soil can be put into good use with the appropriate concentration of fertilizer treatment, thus putting more degraded land under agricultural production and ensuring food security is developing country like Nigeria. The study further suggests a sensitization program for farmers on the appropriate concentration of inorganic fertilizer to be applied to their farmers to avoid pollution.


2017 ◽  
Vol 25 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Taiwo Michael Agbede ◽  
Aruna Olasekan Adekiya ◽  
Ehiokhilen Kevin Eifediyi

AbstractA field experiment was conducted in the forest-savanna transition zone of Nigeria from May to July 2014 and September to November 2015 to determine the impacts of poultry manure (PM) and NPK fertilizer on soil physical properties, and growth and yield of carrot (Daucus carota L.). The five treatments included no manure or fertilizer (control); 10, 20, and 30 megagrams (Mg)·ha-1of PM; and 300 kg·ha-1of 15 N-15 P-15 K fertilizer. All levels of PM reduced soil bulk density and temperature, and improved total porosity and moisture content compared to the NPK fertilizer and the control. Plant height, number of leaves, root diameter, root length, and fresh root yield in the PM and NPK fertilizer treatments were improved compared to the control. Growth and yield parameters of carrot plants treated with 20 and 30 Mg·ha-1PM were higher than the other treatments. The 10 Mg·ha-1PM and NPK fertilizer treatments produced similar growth and yield responses. There was an interaction for year (Y) × fertilizer (F) on plant height, number of leaves, and fresh root yield. Relative to the control 10, 20, or 30 Mg·ha-1PM and NPK fertilizer increased fresh root yield of carrot by 39.9%, 62.0%, 64.9%, and 37.3%, respectively. The 20 Mg·ha-1PM treatment best improved soil properties and carrot productivity as indicated by the benefit-to-cost ratio.


2020 ◽  
Vol 33 (4) ◽  
pp. 1049-1059
Author(s):  
LAURA CRISTINA REZENDE DAS NEVES ◽  
SALOMÃO LIMA GUIMARÃES ◽  
EDNA MARIA BONFIM-SILVA ◽  
ANALY CASTILHO POLIZEL DE SOUZA ◽  
DANIELA TIAGO DA SILVA CAMPOS

ABSTRACT The aim of this study was to evaluate the effect of inoculation with rhizobium and co -inoculation with Azospirillum brasilense on phytometric characteristics of peanut plants grown in Latossolo Vermelho (Oxisol) with and without compaction. The experiment was conducted in a greenhouse, with a randomized block design in a 5x2 factorial scheme and 4 replicates, totaling 40 experimental units. Treatments were composed by commercial inoculant for peanuts (SEMIA 6144 strain); MT 15 strain; combination of MT 15 + A. brasilense; nitrogen control and absolute control. Plant height, number of leaves, number of branches per plant, stem diameter and Falker chlorophyll index were evaluated. The data were subjected to analysis of variance and, when significant, to Tukey test at 1% probability level. For the variables plant height, number of leaves, number of branches and stem diameter, the results were similar for all treatments, except for the absolute control and number of branches at 90 days after emergence (DAE) and stem diameter at 30 and 90 DAE. For stem diameter at 30 DAE, the results were similar to those found in the treatments with co -inoculation, commercial inoculant and nitrogen fertilization. There was interaction for Falker chlorophyll index at 60 DAE, with the best result found in the MT 15 strain. Inoculation with MT15 and co-inoculation with A. brasilense promoted good performance in peanut crop in terms of phytometric characteristics in soil with and without compaction.


Author(s):  
Neveen Anwer Abdalla

The experiment has been conducted in the nursery of the Department of Horticulture and Landscape Design, College of Agriculture, the University of Basrah to study the effect of Spraying foliar of the biostimulants Izomen and Humus on the growth and flowering of Freesia plants. The corms in similar size were planted in pots with a diameter and height of 25 cm, which filled with 2.5 kg of sterilized loam soil. After 50 days of planting, the plants sprayed with Humus at 0, 1.5, 2.5 ml L-1 and after five days sprayed with Izomen at 0, 1.5, 2.5 ml L-1 The different concentrations of biostimulants are sprayed three times, the period between one spray and another 15 is days. The results showed that the spraying of Humus at 2.5 ml L-1 significantly increased the plant height, the number of leaves and the leaf content of chlorophyll recorded (29.56 cm, 8.33 and 58.43%) respectively. Moreover, it is recorded early the flowering date (130 days), and the highest flowering mean is (2.12 inflorescence/plant) and the highest period of the remained flowers on the plant and the vase life (10 and 8 days) respectively. The effects of both Humus and Izomen were similar. In addition to the highest mean of their interaction at 2.5 ml L -1for all the studied traits.


2018 ◽  
Vol 9 (2) ◽  
pp. 153-163
Author(s):  
Wiwik Ambarsari ◽  
Henly Yuliana

The study aimed to determine the effect of the combination of city waste compost and cattle manure on the growth and yield of pakcoy plants (Brassica rapa, L. Chinensis) in Aluvial, Indramayu Regency. The study was conducted at the Experimental Garden of the Faculty of Agriculture, Wiralodra University, Indramayu Regency from March to August 2018. This study used Factorial Pattern Randomized Block Design with two factors. The first factor is city waste compost and the second factor is cattle manure with 4 levels: 0%, 3.0%, 6.0%, and 9.0%, repeated twice. The results showed that there was an independent influence between city waste compost and cattle manure on plant height and number of leaves. The best combination to increase the feasibility weight of pakcoy consumption is by giving city waste compost 9.0% (S4) and cattle manure 6.0% (K3).


2021 ◽  
Vol 8 (7) ◽  
pp. 130
Author(s):  
Simona Sagona ◽  
Baldassare Fronte ◽  
Francesca Coppola ◽  
Elena Tafi ◽  
Matteo Giusti ◽  
...  

β-glucans can activate the animal innate immune system by acting as immune-modulators and inducing various stimulatory effects. The aim of this study was to investigate the effect of 1,3-1,6 β-glucans administered orally for 96 h on Apis mellifera workers (newly emerged and nurse bees). β-glucans were included in honey and syrup. Survival rate and phenoloxidase activity were measured. In both newly emerged and nurse bees, β-glucans supplementation did not affect survival rate (p > 0.05). Conversely, phenoloxidase activity was higher in both newly emerged bees (p = 0.048) and nurse bees (p = 0.014) fed with a honey diet enriched with β-glucans compared to those fed with only honey. In both the newly emerged and nurse bees, no statistical differences in phenoloxidase activity were recorded between the group fed with a syrup-based diet enriched with β-glucans and the control group (p > 0.05). The absence of significant variation in survival suggests that the potential negative effect of β-glucans in healthy bees could be mitigated by their metabolism. Conversely, the inclusion of β-glucans in a honey-based diet determined an increase of phenoloxidase activity, suggesting that the effect of β-glucan inclusion in the diet of healthy bees on phenoloxidase activity could be linked to the type of base-diet. Further investigations on β-glucans metabolism in bees, on molecular mechanism of phenoloxidase activation by 1,3-1,6 β-glucans, and relative thresholds are desirable. Moreover, investigation on the combined action of honey and β-glucans on phenoloxidase activity are needed.


Nabatia ◽  
2016 ◽  
Vol 4 (2) ◽  
pp. 57-63
Author(s):  
M Abror ◽  
M Koko Ardiansyah

This study aims to determine the effect of trimming with a wide variety of growing media hydroponic system fertigation on growth and yield of melon. Research will be conducted in the green house (House of plastic) UMSIDA Agriculture Faculty, Campus 2, Gelam, Candi, Sidoarjo, research time between the months of March to June 2016 using analysis of variance and continued test HSD 5%. The variables measured were plant height, leaf number, level of sweetness of fruit, fruit weight, high-fruit, thickness of the flesh of the fruit, conclusion from this research is going on the effect of trimming and a wide variety of growing media hydroponic system fertigation in treatment PM5 (trimming down, kokopit). There was also a real influence on the treatment PM6 (without pruning, kokopit) .In observation of plant height, leaf number, fruit weight, and high fruit. And also happens to influence a wide range of growing media on growth and yield of melon on hydroponics fertigation system, the PM6 treatment (without pruning, kokopit) had the highest rates in the observation of plant height, weight of the fruit, and the number of leaves.


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