scholarly journals Trihoney improves testicular weight change and histopathological alterations in hypercholesterolemic rabbits

2020 ◽  
pp. 75-87
Author(s):  
Zenab B. Hamad Mohamed ◽  
Hamad Abdulsalam Hamad Alfarisi ◽  
Azantee Yazmie Abdul Wahab ◽  
Azliana binti Abd Fuaat ◽  
Che Anuar Che Mohamad ◽  
...  
Keyword(s):  
New Zealand ◽  
Histopathological Examination ◽  
Testicular Tissue ◽  
Degenerative Changes ◽  
Lipid Lowering ◽  
Cholesterol Diet ◽  
Histopathological Changes ◽  
Histopathological Alterations ◽  
Testicular Weight ◽  
New Zealand White Rabbits

Histopathological examination of testicular tissue is the most reliable and sensitive method for detecting effects on spermatogenesis. Hypercholesterolemia reduces testicular weight, induces testicular degenerative changes, impairs spermatogenesis, affects Leydig and Sertoli cells and induces inflammation and fibrosis of testicular tissue. Based on numerous studies, honey has the ability to improve testicular histopathological abnormalities. To date, whether honey has any protective role against the effects of hypercholesterolemia on male reproductive functions is yet to be explored. This study investigated the effects of Trihoney (a mixture of Trigona, Mellifera and Tualang honeys) on changes in testicular weight and histopathological alterations induced by hypercholesterolemia in male New Zealand white rabbits. These changes were compared with the effects of atorvastatin (a lipid lowering agent) based on the same parameters. Forty-eight male New Zealand white rabbits were assigned into 6 groups and received different diets as follows; Control: commercial pellet; CH: commercial pellet and 0.6 g/kg/day Trihoney; HCD: 1% cholesterol diet; DH1: 1% cholesterol diet and 0.3 g/kg/day Trihoney; DH2: 1% cholesterol diet and 0.6 g/kg/day Trihoney; DAt: 1% cholesterol diet and 2 mg/kg/day atorvastatin. After 12 weeks, blood samples were collected for lipid analysis, the rabbits were sacrificed and the testes were harvested to evaluate any weight and histopathological changes. Administration of 1% cholesterol diet either alone or in combination with atorvastatin caused a significant reduction in the testicular weight, testicular tubular degenerative changes and spermatogenesis impairment. Trihoney, particularly, at the dose of 0.6 g/kg/day improved testicular weight, ameliorated the testicular tubular degenerative changes and enhanced spermatogenesis. The findings of this study suggest that Trihoney plays a favourable role in the protection against testicular weight reduction and histopathological changes induced by hypercholesterolemia. On the other hand, atorvastatin per se may have toxic effects on testicular tissue.

Histopathological changes on the ocular surface of New Zealand white rabbits after instillation of a fixed combination of 0.09 % xanthan gum and 0.1 % chondroitin sulfate

2016 ◽  
Vol 36 (6) ◽  
pp. 841-844 ◽  
Author(s):  
Angélica Y. Contreras-Rubio ◽  
Leopoldo Baiza-Durán ◽  
Oscar Olvera-Montaño ◽  
Mónica Miranda-Robles ◽  
José Bonilla-García ◽  
...  
Keyword(s):  
New Zealand ◽  
Chondroitin Sulfate ◽  
Ocular Surface ◽  
Xanthan Gum ◽  
Fixed Combination ◽  
Histopathological Changes ◽  
New Zealand White Rabbits

Cholesterol feeding impairs endothelium-dependent relaxation of rabbit aorta

1985 ◽  
Vol 63 (9) ◽  
pp. 1206-1209 ◽  
Author(s):  
R. L. Jayakody ◽  
M. P. J. Senaratne ◽  
A. B. R. Thomson ◽  
C. T. Kappagoda
Keyword(s):  
New Zealand ◽  
Thoracic Aorta ◽  
Rabbit Aorta ◽  
Cholesterol Diet ◽  
Cholesterol Feeding ◽  
New Zealand White Rabbits ◽  
Conventional Diet ◽  
Endothelium Dependent Relaxation

Experiments were designed to assess the effect of cholesterol feeding on the endothelium-mediated relaxation of the rabbit aorta to acetylcholine. Age-matched male New Zealand white rabbits were fed either a 2% cholesterol diet or standard rabbit chow. The animals were anaesthetized with sodium pentobarbitone and sacrificed after 4 and 8 weeks on these diets. Rings were prepared from the proximal thoracic aorta and examined in tissue baths. These rings were contracted first with norepinephrine (−6 log mol/L) and acetylcholine was added to demonstrate the endothelium-mediated relaxation. The endothelium-dependent relaxation was significantly less in aortas from rabbits fed the 2% cholesterol diet than in aortas from animals fed the conventional diet. This impairment of relaxation was apparent after both 4 and 8 weeks of cholesterol feeding. In both groups of animals no relaxation was seen in rings from which the endothelium was removed. These results show that cholesterol feeding leads to an impairment of endothelium-mediated relaxation of the rabbit aorta to acetylcholine.

scholarly journals Seasonal histopathological alterations caused by Bothriocephalus acheilognathi in Schizothorax niger of Kashmir valley

2021 ◽  
Vol 17 (2) ◽  
pp. 436-441
Author(s):  
Asifa Wali ◽  
Masood-ul Hassan Balkhi ◽  
Feroz A. Shah ◽  
Farooz Ahmad Bhat ◽  
Bilal Ahmad Bhat ◽  
...  
Keyword(s):  
Host Response ◽  
Degenerative Changes ◽  
Histopathological Changes ◽  
Intestinal Infection ◽  
Kashmir Valley ◽  
Histopathological Alterations ◽  
Cestode Parasite ◽  
Intestinal Contents ◽  
Parasitic Burden ◽  
Bothriocephalus Acheilognathi

Liver and intestines affected by an cestode parasite viz., Bothriocephalus acheilognathi along with their host (Schizothorax niger) were collected randomly. Bothriocephalus acheilognathi causes damage to the intestinal tissues and induce complex host response; the affected fish were anemic and emaciated. The viscera were dark red on opening the abdomen. The intestinal contents showed lot of mucous and contained dark contents. The mucosal wall was red in colour and revealed necrotic surface. The histopathological changes were varied in severity with the season and parasitic burden. Intestinal infection of Bothriocephalus with Adenoscolex was usually seen together. Severe enteritis with heavy infilitration of inflammmtory cells and fibroblasts seen in lamina propia. Liver cells showed severe degenerative changes.

Effects of low-dose ketanserin on atherosclerosis in rats and rabbits

2010 ◽  
Vol 88 (11) ◽  
pp. 1054-1060 ◽  
Author(s):  
Yong-Sheng Yu ◽  
He-Hui Xie ◽  
Ling Li ◽  
Shu-Wei Song ◽  
Ping Han ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vitamin D ◽  
New Zealand ◽  
Treatment Group ◽  
Low Dose ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
New Zealand White Rabbits

The present study was designed to test the hypothesis that a small dose of ketanserin, which enhances baroreflex activity, prevents the early lesions of atherosclerosis. In experiment 1, baroreflex sensitivity (BRS) was measured in 31 spontaneously hypertensive rats (SHRs) in a conscious state using a computerized blood pressure monitoring system. Four weeks later, the rats were administered vitamin D3 and fed a high-cholesterol diet for 8 weeks to induce atherosclerosis. Then their hearts and aortae were removed for pathological examination. A negative correlation was found between BRS and the scores of coronary (r = –0.460, P < 0.01) and aortic atherosclerosis (r = –0.448, P < 0.05) in SHR. In experiment 2, SHRs were divided into 3 groups (n = 10 in each group) and received a dose of ketanserin of 0.3, 1.0, and 3.0 mg/kg (i.g.), respectively. At the smallest dose (0.3 mg/kg), ketanserin did not lower blood pressure but enhanced BRS. In experiment 3, SHRs were administered vitamin D3, fed a high-cholesterol diet, and simultaneously treated with low-dose ketanserin. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (coronary score: 0.90 ± 0.14 vs. 1.76 ± 0.27, P < 0.05; aortic scores: 1.00 ± 0.39 vs. 2.18 ± 0.41, P < 0.05). In experiment 4, male New Zealand White rabbits were fed a high-cholesterol diet and treated with low-dose ketanserin at the same time. The atherosclerosis scores of the treatment group were significantly lower than those of the control group (aortic scores: 0.26 ± 0.20 vs. 0.60 ± 0.31, P < 0.05). In conclusion, the present study demonstrated, for the first time, that low-dose ketanserin prevented the development of atherosclerosis independent of its blood pressure lowering action in SHRs and New Zealand White rabbits at least in part via enhancement of arterial baroreflex function.

scholarly journals Trihoney ameliorates hypercholesterolemia-induced epididymal histopathological changes in male rabbits

2020 ◽  
pp. 90-100 ◽  
Author(s):  
Zenab B. Hamad Mohamed ◽  
Muhammad Bin Ibrahim ◽  
Hamad Abdulsalam Hamad Alfarisi ◽  
Azantee Yazmie Abdul Wahab ◽  
Azliana binti Abd Fuaat ◽  
...  
Keyword(s):  
Weight Change ◽  
Health Benefit ◽  
Reproductive Organs ◽  
The Other ◽  
High Cholesterol Diet ◽  
Protective Agent ◽  
Cholesterol Diet ◽  
Histopathological Changes ◽  
Potential Health ◽  
Histopathological Alterations

Hypercholesterolemia has been linked to weight change and histopathological alteration of male reproductive organs. The epididymis was suggested to be an early target of lipid-related infertility and can be dramatically affected by excess intake of a high cholesterol diet. On the other hand, the interest has been increased towards the use of honey as a prophylactic and therapeutic agent for various diseases. Therefore, the purpose of this study is to investigate the effects of Trihoney (a mixture of Trigona, Mellifera and Tualang) on epididymal weight change and histopathological alterations in hypercholesterolemic male rabbits and compare its effects with atorvastatin. Forty-eight mature male New Zealand white rabbits were divided into 6 groups. Two groups received standard rabbit pellet with 0 and 0.6 g/kg/day of Trihoney respectively while the other four groups received 1% cholesterol diet with 0, 0.3, 0.6 g/kg/day of Trihoney, and 2 mg/kg/day of atorvastatin. After 12 weeks, the rabbits were sacrificed and the epididymides were harvested for evaluation of weight and histopathological changes. Administration of 1% cholesterol diet either alone or in combination with atorvastatin caused a significant reduction in the epididymal weight and epididymal atrophy. Supplementation of Trihoney particularly at the dose of 0.6 g/kg/day improved epididymal weight, regained the normal architecture of the epididymal histology and increased the number of mature sperm inside the tubules of the epididymis. Based on these results, Trihoney exhibited its potential health benefit as a protective agent against epididymal weight reduction and histopathological alterations in hypercholesterolemic rabbits.

Cholesterol diet and effect of long-term withdrawal on plaque development and composition in the thoracic aorta of New Zealand White rabbits

2010 ◽  
Vol 210 (2) ◽  
pp. 407-413 ◽  
Author(s):  
Karen Riedmüller ◽  
Stephan Metz ◽  
Gabriel A. Bonaterra ◽  
Olaf Kelber ◽  
Dieter Weiser ◽  
...  
Keyword(s):  
New Zealand ◽  
Thoracic Aorta ◽  
Cholesterol Diet ◽  
Plaque Development ◽  
New Zealand White Rabbits

Testicular Maturation in Prepubertal New Zealand White Rabbits

1994 ◽  
Vol 31 (5) ◽  
pp. 541-545 ◽  
Author(s):  
S. R. Frame ◽  
M. E. Hurtt ◽  
J. W. Green
Keyword(s):  
Body Weight ◽  
New Zealand ◽  
Study Groups ◽  
Strongly Correlated ◽  
Toxicity Studies ◽  
Testicular Weight ◽  
New Zealand White Rabbits ◽  
Significance Levels ◽  
Testicular Histology ◽  
Age Range

Testicular maturation was assessed in age-matched, sexually immature (13–17-week-old) New Zealand white rabbits using end points frequently evaluated in toxicity studies: testicular weight and testicular histology. Testicular weights and testicular maturity as assessed histologically were markedly variable in sexually immature rabbits, especially at ≥ 14 weeks of age. The large variation in testicular weights in immature rabbits requires that large changes in a treatment group relative to controls be present for statistical detection of testicular weight effects at commonly used significance levels. Testicular weights and testicular maturity were strongly correlated to one another, but neither weight nor maturity was strongly correlated to body weight. Thus, stratification and randomization of immature rabbits to study groups based upon body weight will not assure in absence of group differences with respect to testicular maturity. The large variation in testicular weights and maturity in 13–17-week-old rabbits warrants caution in the evaluation of testicular changes when rabbits of this age range are used in toxicity studies.

A Cytochemical Study of Glucose-6-Phosphatase (G-6-PASE) in Cells Participating in Atherogenesis of Rabbit Aorta

1975 ◽  
Vol 33 ◽  
pp. 460-461
Author(s):  
Sidney D. Kobernick ◽  
Edna A. Elfont ◽  
Neddra L. Brooks
Keyword(s):  
Lipid Metabolism ◽  
New Zealand ◽  
Aortic Wall ◽  
Rabbit Aorta ◽  
Plaque Formation ◽  
Metabolic Changes ◽  
Atherosclerotic Plaques ◽  
Cytochemical Study ◽  
Cellular Alteration ◽  
New Zealand White Rabbits

This cytochemical study was designed to investigate early metabolic changes in the aortic wall that might lead to or accompany development of atherosclerotic plaques in rabbits. The hypothesis that the primary cellular alteration leading to plaque formation might be due to changes in either carbohydrate or lipid metabolism led to histochemical studies that showed elevation of G-6-Pase in atherosclerotic plaques of rabbit aorta. This observation initiated the present investigation to determine how early in plaque formation and in which cells this change could be observed.Male New Zealand white rabbits of approximately 2000 kg consumed normal diets or diets containing 0.25 or 1.0 gm of cholesterol per day for 10, 50 and 90 days. Aortas were injected jin situ with glutaraldehyde fixative and dissected out. The plaques were identified, isolated, minced and fixed for not more than 10 minutes. Incubation and postfixation proceeded as described by Leskes and co-workers.

TO FIND OUT ELECTRO-CARDIOGRAPHIC CHANGES & ECHO CHANGES IN ALUMINIUM PHOSPHIDE AND ETHYLENE DIBROMIDE POISONING

2020 ◽  
Vol 4 (10) ◽  
Author(s):  
Hemant Nargawe ◽  
Sumeet Sisodiya
Keyword(s):  
Biochemical Analysis ◽  
Histopathological Examination ◽  
Common Finding ◽  
Histopathological Changes ◽  
Ethylene Dibromide ◽  
Medical College ◽  
Aluminium Phosphide ◽  
Urine Examination ◽  
Cardiovascular Involvement ◽  
Careful Clinical Examination

Background & Method: The study was conducted in the Department of Medicine Shyam Shah Medical College and Associated Sanjay Gandhi Memorial Hospital, Rewa (M.P). History was followed by a careful clinical examination i.e. cardiovascular, respiratory and gastrointestinal and nervous system. Investigations had done included routine haematological examination, Biochemical analysis, urine examination, ECG, 2 D. Echo & Histopathological examination was done. Result: ST-T changes were most common finding in Aluminium phosphide poisoning in relation to mortality. However hyperkalemia was the most ominous finding associated with 100% mortality, ECG finding in EDB was normal ECG. The most ominous finding was arrhythmia which was associated with 100% mortality. Survivors of ethylene dibromide poisoning echocardiography was normal in 11 (84.61%) followed by pericardial effusion in 2 (15.38%) patients. Conclusion: Noteworthy finding was absence of correlation between cardiovascular involvement, histopathological changes and ECG findings. It was seen that even if ECG showed normal pattern there were significant histopathological changes in heart. Keywords: electro-cardiographic, Aluminium phosphide, ethylene dibromide & poisoning.

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