scholarly journals Statistical diagnostics of postmortem artifacts for determination of ethanol concentration

2019 ◽  
Vol 4 (4) ◽  
pp. 58-62
Author(s):  
German V. Nedugov ◽  
Anatoliy P. Ardashkin ◽  
Vladimir G. Nedugov
Keyword(s):  
Ethanol Concentration ◽  
Alcohol Intoxication ◽  
Regression Equations ◽  
Acute Alcohol Intoxication ◽  
Quantitative Distribution ◽  
Physiological Limits ◽  
Automated Method ◽  
Computational Procedures ◽  
Bladder Urine

Objectives - statistical estimation of the physiological limits of the quantitative distribution of ethanol in cadaveric blood and bladder urine and development of an automated method for diagnosing postmortem artifacts of forensic medical examination of acute alcohol intoxication on the corpse. Material and methods. We performed a retrospective statistical analysis of the results of toxicological determination of ethanol concentration in a blood and in the urine in 2376 deaths from acute alcohol intoxication. Results. Regression equations have been developed that allow us to determine the physiological limits of the quantitative distribution of ethanol in blood and urine with any confidence probability for any degree of alcohol intoxication and phase of acute alcohol intoxication. The set of computational procedures is implemented in the format of the computer program "Alcohol calculator Z 3.0". Conclusion. If the concentrations of ethanol in the blood or in the urine are beyond their physiological limits, the results of toxicological analysis should be considered as a postmortem artifact.

scholarly journals NEW METHOD FOR POSTMORTEM DETERMINATION OF THE AMOUNT OF ALCOHOL TAKEN

2020 ◽  
Author(s):  
German V. Nedugov ◽  
Vladimir G. Nedugov
Keyword(s):  
Mathematical Modeling ◽  
Computer Program ◽  
Ethanol Concentration ◽  
Alcohol Intoxication ◽  
Acute Alcohol Intoxication ◽  
Post Mortem ◽  
Computational Procedures ◽  
First Time ◽  
Ethanol Kinetics

One of the tasks of the forensic medical examination of acute alcohol intoxication on a corpse is to determine the amount of alcohol taken shortly before death. The disadvantage of existing methods for solving this problem is the need to take the liquor by spinal puncture of the corpse and determine the concentration of ethanol in it. This article shows for the first time the possibility of automated post-mortem determination of the amount of alcohol taken without parameter the ethanol concentration indicator in the liquor. Aim. To develop a mathematical model for postmortem determination of the amount of ethanol taken, based on objective parameters of ethanol kinetics and not containing the parameter of ethanol concentration in the liquor, to implement the developed model in the format of a computer program. Material and methods. Regression and simulation mathematical modeling of single-dose ethanol kinetics. Writing the text of a computer program in C#. Results. A set of mathematical models has been developed to enable objective post-mortem determination of the amount of ethanol taken without the need for sampling and chemical-toxicological analysis of the liquor. The set of computational procedures is implemented in the format of the computer program "Alcohol calculator Z 4.0". Conclusions. The developed method of postmortem determination of the amount of ethanol taken and the program "Alcohol calculator Z 4.0" created on its basis are recommended for use in the forensic examination of acute alcohol intoxication on a corpse.

THE INFLUENCE OF VITAMIN C ON EOSINOPHIL RESPONSE TO ACUTE ALCOHOL INTOXICATION IN RATS

1960 ◽  
Vol XXXV (IV) ◽  
pp. 585-593 ◽  
Author(s):  
T. P. J. Vanha-Perttula
Keyword(s):  
Vitamin C ◽  
Ethyl Alcohol ◽  
Intraperitoneal Injection ◽  
Alcohol Intoxication ◽  
Albino Rats ◽  
Acute Alcohol Intoxication ◽  
Alcohol Administration ◽  
Initial Values

ABSTRACT The effect of ethyl alcohol on the circulating eosinophil cells has been studied in female albino rats. An intoxicating dose of alcohol caused a marked depletion of circulating eosinophils which was most clearly evident four hours after the administration of the alcohol. The initial values were not reached before 24 hours had elapsed. Intraperitoneal injection of vitamin C 12 hours prior to the alcohol administration very effectively prevented this eosinopenic reaction. The mechanism of regulation of the eosinophil cells in the circulation has been discussed in the light of previous results and of those obtained in this study.

An Automated Method for the Determination of Serum Calcium with Glyoxal Bis (2-Hydroxyanil)

1967 ◽  
Vol 13 (6) ◽  
pp. 515-520 ◽  
Author(s):  
Genevieve Farese ◽  
Janice L Schmidt ◽  
Milton Mager
Keyword(s):  
Serum Calcium ◽  
Automated Analysis ◽  
Automated Method ◽  
Good Agreement

Abstract A completely automated analysis is described for the determination of serum calcium with glyoxal bis (2-hydroxyanil) solution (GBHA). The method is simple and precise, and the data obtained are in good agreement with results obtained by the manual GBHA procedure.

scholarly journals Automated method for the colorimetric determination of acyl esters in serum

1965 ◽  
Vol 6 (2) ◽  
pp. 301-306
Author(s):  
Arnold Antonis ◽  
D.S. Platt ◽  
J.M. Thorp
Keyword(s):  
Colorimetric Determination ◽  
Automated Method

scholarly journals An HPLC-automated Derivatization for Glutathione and Related Thiols Analysis in Brassica rapa L.

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1157
Author(s):  
Francesco Nacca ◽  
Concetta Cozzolino ◽  
Petronia Carillo ◽  
Pasqualina Woodrow ◽  
Amodio Fuggi ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
High Performance ◽  
Internal Standard ◽  
Plant Tissues ◽  
Cellular Functions ◽  
Sulphur Compounds ◽  
Stress Protection ◽  
Automated Method ◽  
Reduced Forms

The high content of glucosinolates and glutathione makes the Brassicaceae an important healthy food. Thiols and especially glutathione and γ-Glu-Cys-Gly tripeptide are involved in many fundamental cellular functions such as oxidative stress protection. Although several methods for sulphur compounds analysis in biological samples are actually used, the determination of glutathione and other sulphur derivatives in plant tissues is rather problematic due to their extreme susceptibility to oxidation, which can lead to their overestimation. The aim of this work was the improvement and validation of an automated method for determination of reduced and oxidised glutathione, cysteine and γ-glutamylcysteine in plant tissues. The method consists of a fully automated pre-column derivatization of thiols based on monobromobimane reagent, a high-performance liquid chromatography derivatives separation, and a fluorimetric detection and quantification. The method was successfully applied for determination of the oxidized and reduced forms of Cys, γ-GC and GSH content in leaves, petioles, inflorescences and roots of Brassica rapa L. subsp. Sylvestris. At harvest, in freshly cut plants, the average contents of GSH/2GSSG were 840/45, 345/70 and 150/70 nmol g−1 FW for the florets, leaf blades and stems, respectively; those of Cys/2Cys were 80/12, 29/12 and 24/6 nmol g-1 FW; while those of γ-GC/γ-GCCG-γ were 8.0/4.0, and 6.0/3.0, 3.0/2.0 nmol g−1 FW, respectively. Such amounts were lower in low-sulphur-grown plants at harvest. The very low coefficient of variation between repeated tests (maximum 1.6%), the high recovery of internal standard (>96%) and the linear correlation coefficient of the calibration (R2 > 0.99) support the efficiency of this method that allowed analysing about 50 samples/die in a totally automated manner with no operator intervention. Our results show that the reported method integrations can significantly improve thiols detection via HPLC.

Methodology of Estimation of Fire Separation Distances between Construction Facilities by Calculation

2020 ◽  
Vol 1006 ◽  
pp. 93-100
Author(s):  
Vadym Nizhnyk ◽  
Yurii Feshchuk ◽  
Volodymyr Borovykov
Keyword(s):  
Mathematical Model ◽  
Heat Flux ◽  
Linear Regression ◽  
Ignition Temperature ◽  
Oil Products ◽  
Regression Equations ◽  
Fire Load ◽  
Literary Sources ◽  
Tabular Method

Based on analysis of appropriate literary sources we established that estimation of fire separation distances was based of two criteria: heat flux and temperature. We proposed to use “ignition temperature of materials” as principal criterion when determining fire separation distances between adjacent construction facilities. Based on the results derived while performing complete factorial we created mathematical model to describe trend of changing fire separation distances depending on caloric power of fire load (Q), openings factor of the external enclosing structures (k) and duration of irradiation (t); moreover, its adequacy was confirmed. Based on linear regression equations we substantiated calculation and tabular method for the determination of fire separation distances for a facility being irradiated which contains combustible or otherwise non-combustible façade and a facility where liquid oil products turn. We developed and proposed general methodology for estimation of fire separation distances between construction facilities by calculation.

Evaluation of the Neurological Status in Experimental Acute Alcohol Intoxication

2010 ◽  
Vol 149 (3) ◽  
pp. 324-327 ◽  
Author(s):  
E. Y. Bonitenko ◽  
A. N. Grebenyuk ◽  
V. A. Basharin ◽  
M. B. Ivanov ◽  
N. V. Makarova
Keyword(s):  
Alcohol Intoxication ◽  
Neurological Status ◽  
Acute Alcohol Intoxication
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