scholarly journals Uji daya hambat ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumbuhan bakteri Staphylococcus aureus

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Mayangsari P. Rahayu ◽  
Damajanty H. C. Pangemanan ◽  
Christy N. Mintjelungan
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Zone Of Inhibition ◽  
Control Group Design ◽  
Group Design ◽  
Wide Range ◽  
Post Test

Abstract: Mouthwash could inhibit the growth of Staphylococcus aureus bacteria in the oral cavity. Albeit, the most widely used today is mouthwash containing clorhexidine that has side effects in prolonged use. Therefore, it is necessary to find new agents as an alternative antibacterial, especially against Staphylococcus aureus. Squid ink is one of the best known seafood used as alternative medicine which has a wide range of therapeutic applications. This study was aimed to evaluate the inhibitory effect of squid ink extract (Loligo sp) on the growth of S. aureus. This was a true experimental study with a post test only control group design. We used modified Kirby-Bauer method using filter papers. Ciprofloxacin antibacterial was used as the positive control and aquadest as the negative control. Extract of squid ink (Loligo sp) and stock of pure bacteria S. aureus bacteria were prepared. The results showed that mean of zone of inhibition of the squid ink extract (Loligo sp) was 11.22 mm which was less than the zone of inhibition of ciprofloxacin. In conclusion, the squid ink extract (Loligo sp) had a moderate inhibitory effect (Himedia category) on the growth of Staphylococcus aureus.Keywords: extract of squid ink (Loligo sp), Staphylococcus aureus, inhibitory effectAbstrak: Salah satu cara penanganan bakteri Staphylococcus aureus dalam rongga mulut ialah dengan menggunakan obat kumur. Yang banyak digunaakan saat ini yaitu obat kumur yang mengandung clorhexidine dengan efek samping bila digunakan secara berkepanjangan. Oleh karena itudiperlukan penelitian terhadap agen baru sebagai alternatif antibakteri khususnya terhadap bakteri Staphylococcus aureus. Tinta cumi-cumi merupakan salah satu hasil laut yang dikenal dalam dunia pengobatan alternatif serta memiliki jangkauan yang luas pada aplikasi terapeutik. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumuhan bakteri S. aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan kertas saring. Kontrol positif menggunakan antibakteri siprofloksasin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak tinta cumi-cumi (Loligo sp) dan stok bakteri murni S. aureus. Hasil penelitian mendapatkan bahwa diameter rerata zona hambat dari ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumbuhan bakteri S. aureus sebesar 11,22 mm namun diameter tersebut lebih kecil daripada diameter zona hambat siprofloksasin. Simpulan penelitian ini ialah ekstrak tinta cumi-cumi (Loligo sp) memiliki daya hambat kategori sedang (Himedia) terhadap pertumbuhan bakteri S. aureus.Kata kunci: tinta cumi-cumi (Loligo sp), bakteri Staphylococcus aureus, daya hambat

scholarly journals Uji daya hambat ekstrak daun bayam petik (Amaranthus hybridus L.) terhadap pertumbuhan bakteri Staphylococcus aureus

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Oktovianus Pormes ◽  
Damajanty H.C. Pangemanan ◽  
Michael A. Leman
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Amaranthus Hybridus ◽  
Group Design ◽  
Post Test

Abstract: Synthetic antibiotics have certain side effects, therefore, it is necessary to find alternative natural antibacterial materials which is easily available and to be cultivated, inter alia Amaranthus hybridus L. Its leaves contain active compounds, so they might have antibacterial potential. This study was aimed to determine the inhibitory effect of Amaranthus hybridus leaf extract on the growth of Staphylococcus aureus. This was a true experimental study using post test only control group design. This study was conducted at the Microbiology Laboratory of Faculty of Medicine and the Natural Phytochemical Laboratory of Faculty of Mathematics at Sam Ratulangi University. The modified Kirby-Bauer method was used with three wells, containing Amaranthus hybridus leaf extract, the positive control, and the negative control; and with 5 repetitions. The results showed that the average diameters of the inhibition zone of Amaranthus hybridus leaf extract and of the negative control were 0 mm meanwhile of erythromycin as the positive control was 38.8 mm. Conclusion: Amaranthus hybridus leaf extract had no inhibitory effect on Staphylococcus aureus. Keywords: inhibitory zone, Amaranthus hybridus L, Staphylococcus aureus Abstrak: Bahan antibiotik sintetik memiliki efek samping, sehingga perlu dicari bahan alternatif yaitu bahan alami yang mudah didapat dan dibudidayakan, salah satunya ialah bayam petik (Amaranthus hybridus L.). Daun bayam petik memiliki potensi antibakteri karena memiliki kandungan senyawa aktif. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun bayam petik terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran dan Labaratorium Fitokimia Fakultas MIPA Unsrat. Metode pengujian yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan tiga buah sumuran yang diberi ekstrak daun bayam petik, kontrol positif, dan kontrol negatif, sebanyak 5 kali pengulangan. Hasil penelitian menunjukan diameter rerata dari zona hambat yang terbentuk pada sumur dengan ekstrak daun bayam petik dan pada sumur dengan kontrol negatif ialah 0 mm, sedangkan pada sumur yang diberi kontrol positif amoksisilin terjadi resistensi sehingga diganti dengan eritromisin dan didapatkan rerata zona hambat ialah 38,8 mm. Simpulan: Ekstrak daun bayam petik (Amaranthus hybridus L.) tidak memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus.Kata kunci: daya hambat, Amaranthus hybridus L, Staphylococcus aureus

scholarly journals Uji daya hambat getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan Staphylococcus aureus

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Bayu K. Rante ◽  
Youla A. Assa ◽  
Paulina N. Gunawan
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Banana Peel ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Paper Disk ◽  
Chronic Inflammatory Condition ◽  
Post Test

Abstract: Abscess is a chronic inflammatory condition formed by localized infections. In oral cavity abscess, the causal bacteria oftenly found is Staphylococcus aureus. Goroho banana (Musa acuminafe L.) is a typical plant in North Sulawesi. The sap of goroho banana peel contains phytochemicals inter alia flavonoids, saponins, and tannins. This study was aimed to find out whether the sap of goroho banana peel (Musa acuminafe L.) had an inhibitory effect on the growth of Staphylococcus aureus. This was an experimental laboratory study with a post-test only control group design. A modified Kirby-Bauer using paper disk was used as the analytical laboratory method. We used 100% goroho banana peel sap, clindamycin antibiotics as the positive control, and CMCs as the negative control. Staphylococcus aureus bacteria was obtained from the Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado. The results showed that the mean diameter of inhibition zones of goroho banana peel sap was 10.9 mm and was classified as strong inhibition. Conclusion: Goroho banana peel sap had a strong inhibitory effect on the growth of Staphylococcus aureus.Keywords: goroho banana peel sap (Musa acuminafe L.), Staphylococcus aureus, inhibition zone Abstrak: Abses merupakan suatu kondisi inflamasi kronik yang terbentuk dari hasil infeksi yang terlokalisasi. Salah satu bakteri penyebab abses yang sering ditemukan pada rongga mulut ialah Staphylococcus aureus. Pisang goroho merupakan salah satu tanaman khas Sulawesi Utara. Getah kulit buahnya memiliki kandungan fitokimia seperti flavonoid, saponin, dan tanin. Penelitian ini bertujuan untuk mengetahui daya hambat getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ini ialah eksperimental laboratorik dengan post test only control group design. Metode yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan paper disk. Konsentrasi getah buah pisang goroho yang digunakan yaitu 100%, kontrol positif menggunakan antibiotik klindamisin, dan kontrol negatif menggunakan CMC. Bakteri Staphylococcus aurues diambil dari stok bakteri murni Laboratorium Mikrobiologi Fakultas MIPA Unsrat Manado. Hasil penelitian menunjukkan diameter rerata zona hambat dari getah kulit buah pisang goroho yang terbentuk ialah 10,9 mm dan digolongkan sebagai zona hambat kuat. Simpulan: Getah kulit buah pisang goroho memiliki daya hambat yang kuat terhadap pertumbuhan Staphylococcus aureus.Kata kunci: getah kulit buah pisang goroho (Musa acuminafe L.), Staphylococcus aureus, zona hambat

scholarly journals Uji Daya Hambat Perasan Daging Buah Alpukat (Persea americana Mill.) terhadap Pertumbuhan Bakteri Staphylococcus aureus

e-GIGI ◽  
2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Dwi S.R. Muchyar ◽  
Damajanty H.C. Pangemanan ◽  
Aurelia S.R. Supit
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Persea Americana ◽  
Control Group ◽  
Control Group Design ◽  
Study Program ◽  
Group Design ◽  
Post Test ◽  
Strong Inhibitory Effect

Abstract: To date, Staphylococcus aureus resistance to some antibiotics is still increasing inter alia the methisillin-resistant Staphylococcus aureus (MRSA). Therefore, it is necessary to find other alternative materials that could overcome this bacteria. Avocado (Persea americana Mill.) is a medicinal plant that contains antibacterial compounds such as saponins, glutathiones, flavonoids, and tannins in its fruit flesh. This study was aimed to obtain the inhibitory effect of avocado flesh on the growth of S. aureus. This was a true experimental study with a post test only control group design. We used Kirby-Bauer modification with paper disks. The positive control was ciprofloxacin and the negative control was aquadest. The avocado flesh was refined by using a juicer and then was filtered. Staphylococcus aureus bacteria were obtained from pure bacteria stock at Microbiology Laboratory of Pharmacy Study Program at Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The result showed that the avocado juice had an inhibitory effect on the growth of S. aureus. The mean diameter of inhibitory zones formed was 15.55 mm which was classified as a strong inhibitory effect. Conclusion: The avocado flesh had a strong inhibitory effect on the growth of Staphylococcus aureus.Keywords: avocado flesh (Persea americana Mill.), S. aureus, inhibitory effect Abstrak: Resistensi bakteri Staphylococcus aureus terhadap beberapa jenis antibiotik sudah cukup tinggi. Sebagai contoh ialah methicillin-resistant Staphylococcus aureus (MRSA). Oleh karena itu perlu dicari bahan alternatif lain yang dapat mengatasi berkembang biaknya bakteri ini. Buah alpukat (Persea americana Mill.) merupakan salah satu tanaman obat yang dikenal berkhasiat sebagai antibakteri karena terdapat kandungan senyawa antibakteri pada daging buah seperti saponin, glutatin, flavonoid, dan tanin. Penelitian ini bertujuan untuk mengetahui daya hambat perasan daging buah alpukat (Persea americana Mill.) terhadap pertumbuhan bakteri S. aureus. Jenis penelitian ini ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu modifikasi Kirby-Bauer dengan kertas saring. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Daging buah alpukat dihaluskan dengan menggunakan juicer dan disaring. Bakteri S. aureus diambil dari stok bakteri murni Laboratorium Mikrobiologi Program Studi Farmasi Fakultas MIPA Universitas Sam Ratulangi. Hasil penelitian menunjukkan bahwa perasan daging buah alpukat memilki daya hambat terhadap pertumbuhan bakteri S. aureus. Diameter rerata zona hambat yang terbentuk yaitu 15,55 mm dan digolongkan sebagai zona hambat yang kuat. Simpulan: Perasan daging buah alpukat (Persea americana Mill.) memiliki daya hambat kuat terhadap pertumbuhan bakteri S. aureus.Kata kunci: daging buah alpukat (Persea americana Mill.), S. aureus, daya hambat

scholarly journals Uji Daya Hambat Ekstrak Ikan Nike (Awous melanocephalus) Terhadap Pertumbuhan Bakteri Fusobacterium nucleatum

e-GIGI ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 238
Author(s):  
Stevia E. Nonutu ◽  
Damajanty H. C. Pangemanan ◽  
Christy N. Mintjelungan
Keyword(s):  
Side Effects ◽  
Treatment Options ◽  
Antibacterial Properties ◽  
Fusobacterium Nucleatum ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design

Abstract: One of the treatment options of periodontal abscess caused by Fusobacterium nucleatum is administration of antibiotics. However, long-term antibiotics consumption can cause negative side effects. Therefore, alternative treatments that have low side effects and easy to be obtained are needed. Nike fish (Awaous melanocephalus) is one of the endemic fish of North Sulawesi province which has antibacterial properties. This study was aimed to evaluate the inhibition effect of nike fish extract on the growth of Fusobacterium nucleatum. This was a true experimental study with a posttest only control group design. We used modified Kirby-Bauer method with filter papers. Ciprofloxacin was used as the positive control and aquadest as the negative control. Extract of nike fish and stock of pure bacteria Fusobacterium nucleatum were prepared. The results showed that the average diameters of the inhibition zones formed in the nike fish extract after three repetitions, were as follows: for extract concentration of 12.5% was 2.91 mm; 25% was 4.16 mm; 50% was 8.41 mm; and 100% was 9.58 mm. In conclusion, nike fish extract (Awaous melanocephalus) at concentrations of 50% and 100% had a weak inhibitory effect (Himedia category) on the growth of Fusobacterium nucleatum meanwhile at concentrations of 12.5% and 25% there was no activity of zone of inhibition.Keywords: extract of nike fish (Awaous melanocephalus); Fusobacterium nucleatum; inhibitory effect Abstrak: Salah satu opsi pengobatan abses periodontal yang disebabkan oleh bakteri Fusobacterium nucleatum yaitu dengan penggunaan antibiotik namun mengonsumsi antibiotik jangka panjang dapat menimbulkan efek samping negatif. Oleh karena itu, diperlukan pengobatan alternatif yang memiliki efek samping rendah serta mudah didapat. Ikan nike merupakan salah satu ikan endemik Provinsi Sulawesi Utara yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak ikan nike (Awaous melanocephalus) terhadap pertumbuhan bakteri Fusobacterium nucleatum. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan paper disk. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak ikan nike dan stok bakteri murni Fusobacterium nucleatum. Hasil penelitian menunjukkan bahwa rerata diameter zona hambat yang terbentuk pada ekstrak ikan nike setelah tiga kali pengulangan yaitu untuk konsentrasi 12,5% sebesar 2,91 mm; 25% sebesar 4,16 mm; 50% sebesar 8,41 mm; dan 100% sebesar 9,58 mm. Simpulan penelitian ini ialah ekstrak ikan nike (Awaous melanocephalus) pada konsentrasi 50% dan 100% memiliki daya hambat kategori lemah (Himedia) terhadap pertumbuhan bakteri Fusobacterium nucleatum sedangkan pada konsentrasi 12,5% dan 25% dikategorikan tidak terdapat aktivitas zona hambat. Kata kunci: ekstrak ikan nike (Awaous melanocephalus); Fusobacterium nucleatum; daya hambat

scholarly journals UJI EFEKTIFITAS BIOLARVASIDA EKSTRAK ETANOL BUAH LAMPESU (Baccaurea lanceolata) TERHADAP LARVA INSTAR III Culex quinquefasciatus

2020 ◽  
Vol 3 (3) ◽  
pp. 7
Author(s):  
Dea Alfani Nandjan
Keyword(s):  
Culex Quinquefasciatus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Chemical Insecticide ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Infection Disease

Filariasis is chronic infection disease caused by worm and carried by Culex quinquefasciatus mosquito. One of theways to controlling mosquitoes vectors are use biolarvacide or chemical insecticide. Chemical insecticide causingresistance Culex quinquefasciatus larvae and toxic for human. This research aim to determine the biolarvacideeffectiviteness of ethanol extract lampesu fruit (Baccaurea lanceolata) to Culex quinquefasciatus larvae instars III.This study was true experimental with a Post test-only control group design. This study used 700 larvae instars III ofCulex quinquefasciatus divided into 7 groups consentration of 0,2%, 0,4%, 0,6%, 0,8% and 1%, the positive control(abate) and negative control (aquadest). The observation was did after treatment in 3 hours, 6 hours, 12  hours and 24hours. The experiment is replicated four times. At 24 hours exposure concentration of 0,6% the test larvae mortalityreached 38% and at concentration of 1% the test larvae mortality reached 30%. In this stuy the concentration of1,531 % was effective to kill larvae with of 50% mortality(LC50) and concentration of 10,729 % was effective to killlarvae with of 90% mortality(LC90). Ethanol Ekstract of Lampesu Fruit (Baccaurea lanceolata) not effective asbiolarvacide ofCulex quinquefasciatus larvae instars III.

scholarly journals PENGARUH EKSTRAK AKAR ENCOK TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Igede Sudarmanto
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Statistic Analysis ◽  
Control Group Design ◽  
Inhibition Zone Diameter ◽  
Group Design ◽  
The Mean

Background: Resistance is defined as growth inhibition of bacteria with antibiotics. Staphylococcus aureus bacteria is known to be resistant to gentamicin and methicillin. Staphylococcus aureus can cause various infection of the skin, injuries, and system. Methods: This research is true-experimental with Posttest-Only Control Group Design and using Kirby-Bauer method by means of five concentration (20, 40, 60, 80, dan 100%). The negative control uses sterile distilled water, and the positive control uses Vancomycin 30 µg. Result: This research showed the mean of inhibition zone diameter of each concentration consecutively 16 mm, 20 mm, 22 mm, 23 mm, and 23 mm. Oneway ANOVA statistic analysis showed that the value of p is 0,000 that mean there is an influence of encok roots extract to Staphylococcus aureus growth. Conclusion: There is an influence of encok roots extract to Staphylococcus aureus growth. Seen from its ability to inhibit the growth of Staphylococcus aureus.

scholarly journals Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Zuraida Zuraida ◽  
Eti Yerizel ◽  
Eliza Anas
Keyword(s):  
Vitamin C ◽  
Negative Control Group ◽  
Organ Damage ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstrak Pemberian rosella (Hibiscus sabdariffa Linn) diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4). Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA). Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn) terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4), perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb) dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb). Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05). Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn) is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn) on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr. Samples were taken at random and divided into 4 groups consisting of a negative control group, positive control (CCl4), treatment 1 (CCl4 and roselle extract 250 mg / kg bw) and treatment 2 (CCl4 and roselle extract 500 mg / kg bw). CCl4 was given a single dose orally, after 24 hours, the subjects were given rosella extract orally for 14 days. Data were analyzed by ANOVA with a confidence level of 95%. Rosella extract obtained statistically significant differences of MDA and catalase levels among groups (p <0.05). It can be concluded that the rosella extract can reduce levels of MDA and increase the activity of catalase mice exposed to CCl4. From the research it can be concluded that the rosella extract can reduce levels of MDA and increas the activity of catalase mice exposed to CCl4. It is needed further research on the toxicity of extracts of rosella and organ damage caused.Keywords: carbon tetrachloride, MDA, catalase, rosella

scholarly journals Antibacterial activity lemongrass leaves of Staphylococcus aureus inhibition one

2020 ◽  
Vol 5 (2) ◽  
pp. 176-183
Author(s):  
Yunita Yunita ◽  
Fitria Lestari ◽  
Yuli Febrianti
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Positive Control ◽  
Control Group ◽  
Skin Infections ◽  
Control Group Design ◽  
Group Design ◽  
Cymbopogon Nardus ◽  
Post Test

Staphylococcus aureus is a nosocomial problem that can cause skin infections. Treatment of skin infections can be done by using citronella (Cymbopogon nardus) which is antibacterial because it contains compounds such as flavonoids, steroids, terpenoids, saponins, and essential oils. This study aims to determine the antibacterial power of Cymbopogon nardus leaf starch essence against Staphylococcus aureus inhibition zone. This type of research is a laboratory experiment using a post-test only control group design. The results showed that the extract of Cymbopogon nardus leaves with a concentration of 5%, 10%, 15%, and 20% with positive control of ampicillin had different inhibitory zona against Staphylococcus aureus bacteria. The conclusion is that the concentration of Cymbopogon nardus leaf starch is the most effective and forms an inhibition zone with the largest average (16.28), namely a concentration of 20%.Keywords: Antibacterial, lemongrass, Staphylococcus aureus 

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Sadakata Sinulingga ◽  
Subandrate Subandrate ◽  
Bebbi Arisya Kesumaputri ◽  
Galuh Anggraini
Keyword(s):  
Bleeding Time ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Piper Betle ◽  
Control Group Design ◽  
Minimum Concentration ◽  
Group Design ◽  
Hemostatic Effect

Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Mice’s tail was cut at diameter 3 mm and pretest bleeding time was counted. Mice’s tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.

Sign in / Sign up

Export Citation Format

Share Document