scholarly journals Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Zuraida Zuraida ◽  
Eti Yerizel ◽  
Eliza Anas
Keyword(s):  
Vitamin C ◽  
Negative Control Group ◽  
Organ Damage ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstrak Pemberian rosella (Hibiscus sabdariffa Linn) diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4). Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA). Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn) terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4), perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb) dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb). Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05). Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn) is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn) on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr. Samples were taken at random and divided into 4 groups consisting of a negative control group, positive control (CCl4), treatment 1 (CCl4 and roselle extract 250 mg / kg bw) and treatment 2 (CCl4 and roselle extract 500 mg / kg bw). CCl4 was given a single dose orally, after 24 hours, the subjects were given rosella extract orally for 14 days. Data were analyzed by ANOVA with a confidence level of 95%. Rosella extract obtained statistically significant differences of MDA and catalase levels among groups (p <0.05). It can be concluded that the rosella extract can reduce levels of MDA and increase the activity of catalase mice exposed to CCl4. From the research it can be concluded that the rosella extract can reduce levels of MDA and increas the activity of catalase mice exposed to CCl4. It is needed further research on the toxicity of extracts of rosella and organ damage caused.Keywords: carbon tetrachloride, MDA, catalase, rosella

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals UJI EFEKTIFITAS BIOLARVASIDA EKSTRAK ETANOL BUAH LAMPESU (Baccaurea lanceolata) TERHADAP LARVA INSTAR III Culex quinquefasciatus

2020 ◽  
Vol 3 (3) ◽  
pp. 7
Author(s):  
Dea Alfani Nandjan
Keyword(s):  
Culex Quinquefasciatus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Chemical Insecticide ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Infection Disease

Filariasis is chronic infection disease caused by worm and carried by Culex quinquefasciatus mosquito. One of theways to controlling mosquitoes vectors are use biolarvacide or chemical insecticide. Chemical insecticide causingresistance Culex quinquefasciatus larvae and toxic for human. This research aim to determine the biolarvacideeffectiviteness of ethanol extract lampesu fruit (Baccaurea lanceolata) to Culex quinquefasciatus larvae instars III.This study was true experimental with a Post test-only control group design. This study used 700 larvae instars III ofCulex quinquefasciatus divided into 7 groups consentration of 0,2%, 0,4%, 0,6%, 0,8% and 1%, the positive control(abate) and negative control (aquadest). The observation was did after treatment in 3 hours, 6 hours, 12  hours and 24hours. The experiment is replicated four times. At 24 hours exposure concentration of 0,6% the test larvae mortalityreached 38% and at concentration of 1% the test larvae mortality reached 30%. In this stuy the concentration of1,531 % was effective to kill larvae with of 50% mortality(LC50) and concentration of 10,729 % was effective to killlarvae with of 90% mortality(LC90). Ethanol Ekstract of Lampesu Fruit (Baccaurea lanceolata) not effective asbiolarvacide ofCulex quinquefasciatus larvae instars III.

scholarly journals Uji daya hambat ekstrak daun bayam petik (Amaranthus hybridus L.) terhadap pertumbuhan bakteri Staphylococcus aureus

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Oktovianus Pormes ◽  
Damajanty H.C. Pangemanan ◽  
Michael A. Leman
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Amaranthus Hybridus ◽  
Group Design ◽  
Post Test

Abstract: Synthetic antibiotics have certain side effects, therefore, it is necessary to find alternative natural antibacterial materials which is easily available and to be cultivated, inter alia Amaranthus hybridus L. Its leaves contain active compounds, so they might have antibacterial potential. This study was aimed to determine the inhibitory effect of Amaranthus hybridus leaf extract on the growth of Staphylococcus aureus. This was a true experimental study using post test only control group design. This study was conducted at the Microbiology Laboratory of Faculty of Medicine and the Natural Phytochemical Laboratory of Faculty of Mathematics at Sam Ratulangi University. The modified Kirby-Bauer method was used with three wells, containing Amaranthus hybridus leaf extract, the positive control, and the negative control; and with 5 repetitions. The results showed that the average diameters of the inhibition zone of Amaranthus hybridus leaf extract and of the negative control were 0 mm meanwhile of erythromycin as the positive control was 38.8 mm. Conclusion: Amaranthus hybridus leaf extract had no inhibitory effect on Staphylococcus aureus. Keywords: inhibitory zone, Amaranthus hybridus L, Staphylococcus aureus Abstrak: Bahan antibiotik sintetik memiliki efek samping, sehingga perlu dicari bahan alternatif yaitu bahan alami yang mudah didapat dan dibudidayakan, salah satunya ialah bayam petik (Amaranthus hybridus L.). Daun bayam petik memiliki potensi antibakteri karena memiliki kandungan senyawa aktif. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak daun bayam petik terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Penelitian dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran dan Labaratorium Fitokimia Fakultas MIPA Unsrat. Metode pengujian yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan tiga buah sumuran yang diberi ekstrak daun bayam petik, kontrol positif, dan kontrol negatif, sebanyak 5 kali pengulangan. Hasil penelitian menunjukan diameter rerata dari zona hambat yang terbentuk pada sumur dengan ekstrak daun bayam petik dan pada sumur dengan kontrol negatif ialah 0 mm, sedangkan pada sumur yang diberi kontrol positif amoksisilin terjadi resistensi sehingga diganti dengan eritromisin dan didapatkan rerata zona hambat ialah 38,8 mm. Simpulan: Ekstrak daun bayam petik (Amaranthus hybridus L.) tidak memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus.Kata kunci: daya hambat, Amaranthus hybridus L, Staphylococcus aureus

scholarly journals Potential of Cocoa Ethanol Extract Toward Nematocyst Tubul Firing Inhibition of Physalia Utriculus Toxin In Vitro

2019 ◽  
Vol 5 (2) ◽  
pp. 30
Author(s):  
Sarwendah Siswi Winasis ◽  
Al Munawir ◽  
Adelia Handoko
Keyword(s):  
Normal Control ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Average Percentage ◽  
Post Test

There was estimated 150 million envenomation cases due to jellyfish stings occur globally every year. 100 from 10,000 jellyfish species in the world known to be dangerous, one them is Physalia utriculus. The aim of this study was to determine the potential of cocoa (Theobroma cacao L.) ethanol extract toward nematocyst tubul firing inhibition of jellyfish (Physalia utriculus) toxin in vitro. The method was true experimental design with post test only control group design. The study divided into 8 groups: 1 normal control, 1 positive control, 1 negative control, and 5 treatments grups by giving cocoa ethanol extract with concentration 20%, 2%, 0.2%, 0.02%, 0.002%. The observation was made by calculating the percentage number of firing nematocysts. The result showed average percentage of firing nematocyst in the normal control group was 42.50 ± 3.18, positive control group was 37.97 ± 5.57, negative control group was 52.44 ± 2.98, and treatment group which given with cocoa ethanol extract 20%, 2%, 0.2%, 0.02%, 0.002% were 48.24 ± 5.37; 40.62 ± 7.10; 29.45 ± 5.39; 37.60 ± 9.78; 41.11 ± 3.92, respectively. The One Way Annova statistical results test show significance value 0.001 (p≤0.05). The conclusion of this study was the cocoa ethanol extract with concentration 0.2% has most potential to inhibit the jellyfish (P. utriculus) nematocyst tubule firing in vitro. Keywords: Physalia utriculus, cocoa, toxin, jellyfish

scholarly journals The effect of e-cigarette and a conventional cigarette to the alveolus on Wistar male rats

2019 ◽  
Vol 8 (4) ◽  
pp. 251
Author(s):  
Budian Nurpangestu ◽  
Yusrotun Kharimah ◽  
Fita Linggasati ◽  
Moch Bahrudin
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Male Rats ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Wistar Strain ◽  
Post Test ◽  
Wistar Male Rats

Smoking can cause a variety of diseases one of them on pulmonary organs, e-cigarettes are also considered to be safer than conventional cigarettes and the number of smokers in Indonesia is increasing every year. The purpose of this study to determine differences in pulmonary alveolar damage in male rats (Rattus norvegicus Wistar strain) on electric cigarette smoke exposure and conventional cigarettes. This research is true experimental with post-test only control group design. Male rats were used as many as 25 rats were divided into 5 groups, namely the group P0 as a negative control group; P1 group was exposed to clove cigarettes; P2 group is exposed to a filter cigarette; P3 group was exposed to e-cigarette 0mg; P4 group exposed the e-cigarette 3mg. Samples were treated for 30 days. Data were analyzed using Kruskal Wallis with p &lt;0.05. The results showed that there were significant differences in alveolar damage in the fifth group (p = 0.003). With Mann Whitney shows that groups P1, P2, P3, and P4 there are no significant differences. The conclusion of this study is there is no difference in alveolar damage on Wistar male rats by e-cigarettes and conventional cigarettes exposure.

scholarly journals Uji daya hambat ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumbuhan bakteri Staphylococcus aureus

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Mayangsari P. Rahayu ◽  
Damajanty H. C. Pangemanan ◽  
Christy N. Mintjelungan
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Zone Of Inhibition ◽  
Control Group Design ◽  
Group Design ◽  
Wide Range ◽  
Post Test

Abstract: Mouthwash could inhibit the growth of Staphylococcus aureus bacteria in the oral cavity. Albeit, the most widely used today is mouthwash containing clorhexidine that has side effects in prolonged use. Therefore, it is necessary to find new agents as an alternative antibacterial, especially against Staphylococcus aureus. Squid ink is one of the best known seafood used as alternative medicine which has a wide range of therapeutic applications. This study was aimed to evaluate the inhibitory effect of squid ink extract (Loligo sp) on the growth of S. aureus. This was a true experimental study with a post test only control group design. We used modified Kirby-Bauer method using filter papers. Ciprofloxacin antibacterial was used as the positive control and aquadest as the negative control. Extract of squid ink (Loligo sp) and stock of pure bacteria S. aureus bacteria were prepared. The results showed that mean of zone of inhibition of the squid ink extract (Loligo sp) was 11.22 mm which was less than the zone of inhibition of ciprofloxacin. In conclusion, the squid ink extract (Loligo sp) had a moderate inhibitory effect (Himedia category) on the growth of Staphylococcus aureus.Keywords: extract of squid ink (Loligo sp), Staphylococcus aureus, inhibitory effectAbstrak: Salah satu cara penanganan bakteri Staphylococcus aureus dalam rongga mulut ialah dengan menggunakan obat kumur. Yang banyak digunaakan saat ini yaitu obat kumur yang mengandung clorhexidine dengan efek samping bila digunakan secara berkepanjangan. Oleh karena itudiperlukan penelitian terhadap agen baru sebagai alternatif antibakteri khususnya terhadap bakteri Staphylococcus aureus. Tinta cumi-cumi merupakan salah satu hasil laut yang dikenal dalam dunia pengobatan alternatif serta memiliki jangkauan yang luas pada aplikasi terapeutik. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumuhan bakteri S. aureus. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan kertas saring. Kontrol positif menggunakan antibakteri siprofloksasin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak tinta cumi-cumi (Loligo sp) dan stok bakteri murni S. aureus. Hasil penelitian mendapatkan bahwa diameter rerata zona hambat dari ekstrak tinta cumi-cumi (Loligo sp) terhadap pertumbuhan bakteri S. aureus sebesar 11,22 mm namun diameter tersebut lebih kecil daripada diameter zona hambat siprofloksasin. Simpulan penelitian ini ialah ekstrak tinta cumi-cumi (Loligo sp) memiliki daya hambat kategori sedang (Himedia) terhadap pertumbuhan bakteri S. aureus.Kata kunci: tinta cumi-cumi (Loligo sp), bakteri Staphylococcus aureus, daya hambat

scholarly journals Uji Daya Hambat Ekstrak Ikan Nike (Awous melanocephalus) Terhadap Pertumbuhan Bakteri Fusobacterium nucleatum

e-GIGI ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 238
Author(s):  
Stevia E. Nonutu ◽  
Damajanty H. C. Pangemanan ◽  
Christy N. Mintjelungan
Keyword(s):  
Side Effects ◽  
Treatment Options ◽  
Antibacterial Properties ◽  
Fusobacterium Nucleatum ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Group Design

Abstract: One of the treatment options of periodontal abscess caused by Fusobacterium nucleatum is administration of antibiotics. However, long-term antibiotics consumption can cause negative side effects. Therefore, alternative treatments that have low side effects and easy to be obtained are needed. Nike fish (Awaous melanocephalus) is one of the endemic fish of North Sulawesi province which has antibacterial properties. This study was aimed to evaluate the inhibition effect of nike fish extract on the growth of Fusobacterium nucleatum. This was a true experimental study with a posttest only control group design. We used modified Kirby-Bauer method with filter papers. Ciprofloxacin was used as the positive control and aquadest as the negative control. Extract of nike fish and stock of pure bacteria Fusobacterium nucleatum were prepared. The results showed that the average diameters of the inhibition zones formed in the nike fish extract after three repetitions, were as follows: for extract concentration of 12.5% was 2.91 mm; 25% was 4.16 mm; 50% was 8.41 mm; and 100% was 9.58 mm. In conclusion, nike fish extract (Awaous melanocephalus) at concentrations of 50% and 100% had a weak inhibitory effect (Himedia category) on the growth of Fusobacterium nucleatum meanwhile at concentrations of 12.5% and 25% there was no activity of zone of inhibition.Keywords: extract of nike fish (Awaous melanocephalus); Fusobacterium nucleatum; inhibitory effect Abstrak: Salah satu opsi pengobatan abses periodontal yang disebabkan oleh bakteri Fusobacterium nucleatum yaitu dengan penggunaan antibiotik namun mengonsumsi antibiotik jangka panjang dapat menimbulkan efek samping negatif. Oleh karena itu, diperlukan pengobatan alternatif yang memiliki efek samping rendah serta mudah didapat. Ikan nike merupakan salah satu ikan endemik Provinsi Sulawesi Utara yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak ikan nike (Awaous melanocephalus) terhadap pertumbuhan bakteri Fusobacterium nucleatum. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Metode yang digunakan yaitu metode modifikasi Kirby-Bauer dengan menggunakan paper disk. Kontrol positif menggunakan antibakteri ciprofloxacin dan kontrol negatif menggunakan akuades. Pada penelitian ini digunakan ekstrak ikan nike dan stok bakteri murni Fusobacterium nucleatum. Hasil penelitian menunjukkan bahwa rerata diameter zona hambat yang terbentuk pada ekstrak ikan nike setelah tiga kali pengulangan yaitu untuk konsentrasi 12,5% sebesar 2,91 mm; 25% sebesar 4,16 mm; 50% sebesar 8,41 mm; dan 100% sebesar 9,58 mm. Simpulan penelitian ini ialah ekstrak ikan nike (Awaous melanocephalus) pada konsentrasi 50% dan 100% memiliki daya hambat kategori lemah (Himedia) terhadap pertumbuhan bakteri Fusobacterium nucleatum sedangkan pada konsentrasi 12,5% dan 25% dikategorikan tidak terdapat aktivitas zona hambat. Kata kunci: ekstrak ikan nike (Awaous melanocephalus); Fusobacterium nucleatum; daya hambat

scholarly journals Efek Pemberian Vitamin C terhadap Mikroskopis Ginjal Tikus Wistar yang Terpapar Plumbum Asetat

2017 ◽  
Vol 6 (2) ◽  
pp. 417
Author(s):  
Zaki Farhan ◽  
M. Setia Budi ◽  
Elmatris Syamsir
Keyword(s):  
Vitamin C ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
C 50

Plumbum merupakan salah satu logam berat yang terkandung dalam bahan bakar kendaraan bermotor. Plumbum diekskresikan melalui ginjal sehingga dalam jangka waktu yang lama akan menyebabkan kerusakan sel epitel tubulus proksimal. Vitamin C merupakan salah satu senyawa yang bersifat antioksidan yang dapat melindungi efek radikal bebas yang ditimbulkan oleh plumbum. Tujuan penelitian ini adalah menentukan efek pemberian vitamin C terhadap mikroskopis ginjal tikus Wistar yang terpapar plumbum asetat. Penelitian ini merupakan studi eksperimental post test only control group design. Subjek penelitian adalah 24 ekor tikus Wistar jantan yang dibagi menjadi empat kelompok, yaitu K- tanpa perlakuan, K+ dengan pemberian Pb asetat 50 mg/kgbb/hari, P1 dengan pemberian Pb asetat dan vitamin C 50 mg/kgbb/hari, dan P2 dengan pemberian Pb asetat dan vitamin C 75 mg/kgbb/hari. Masing- masing kelompok terdiri dari enam ekor tikus dengan perlakuan selama empat minggu. Hasil penelitian menunjukkan bahwa terjadi kerusakan tubulus proksimal ginjal yang berbeda secara bermakna pada kelompok K+, P1, P2 dibandingkan dengan kelompok K- serta perbedaan yang bermakna pada kelompok P1, P2 terhadap kelompok K+ (p < 0,05). Terdapat efek pemberian vitamin C terhadap mikroskopis ginjal tikus Wistar terhadap paparan  plumbum asetat dengan dosis 50 mg/kgbb/hari. Simpulan hasil penelitian ini adalah terjadi perubahan mikroskopis ginjal tikus Wistar yang terpapar plumbum asetat dengan dosis 50 mg/kgbb/hari.

scholarly journals Efek Pemberian Vitamin C terhadap Mikroskopis Ginjal Tikus Wistar yang Terpapar Plumbum Asetat

2017 ◽  
Vol 6 (2) ◽  
pp. 417
Author(s):  
Zaki Farhan ◽  
M. Setia Budi ◽  
Elmatris Syamsir
Keyword(s):  
Vitamin C ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
C 50

Plumbum merupakan salah satu logam berat yang terkandung dalam bahan bakar kendaraan bermotor. Plumbum diekskresikan melalui ginjal sehingga dalam jangka waktu yang lama akan menyebabkan kerusakan sel epitel tubulus proksimal. Vitamin C merupakan salah satu senyawa yang bersifat antioksidan yang dapat melindungi efek radikal bebas yang ditimbulkan oleh plumbum. Tujuan penelitian ini adalah menentukan efek pemberian vitamin C terhadap mikroskopis ginjal tikus Wistar yang terpapar plumbum asetat. Penelitian ini merupakan studi eksperimental post test only control group design. Subjek penelitian adalah 24 ekor tikus Wistar jantan yang dibagi menjadi empat kelompok, yaitu K- tanpa perlakuan, K+ dengan pemberian Pb asetat 50 mg/kgbb/hari, P1 dengan pemberian Pb asetat dan vitamin C 50 mg/kgbb/hari, dan P2 dengan pemberian Pb asetat dan vitamin C 75 mg/kgbb/hari. Masing- masing kelompok terdiri dari enam ekor tikus dengan perlakuan selama empat minggu. Hasil penelitian menunjukkan bahwa terjadi kerusakan tubulus proksimal ginjal yang berbeda secara bermakna pada kelompok K+, P1, P2 dibandingkan dengan kelompok K- serta perbedaan yang bermakna pada kelompok P1, P2 terhadap kelompok K+ (p < 0,05). Terdapat efek pemberian vitamin C terhadap mikroskopis ginjal tikus Wistar terhadap paparan  plumbum asetat dengan dosis 50 mg/kgbb/hari. Simpulan hasil penelitian ini adalah terjadi perubahan mikroskopis ginjal tikus Wistar yang terpapar plumbum asetat dengan dosis 50 mg/kgbb/hari.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

Sign in / Sign up

Export Citation Format

Share Document