scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals POTENTIAL ETHANOL OF Carica papaya L. EXTRACT AS IMMUNOMODULATORY THROUGH HISTOLOGY OBSERVATION AT MICE BALB/C SPLEEN

2017 ◽  
Vol 13 (2) ◽  
pp. 205
Author(s):  
Sri Tasminatun ◽  
Ria Pravitasari ◽  
SN. Nurul Makiyah
Keyword(s):  
Treatment Group ◽  
Carica Papaya ◽  
Negative Control Group ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
One Way Anova

Abstract: The diameter of the alba pulp of the spleen will be enlarged, when the body responds to allergens by increasing the activity of the immune system. Papaya fruits (Carica papaya L.) containing flavonoid that can be as an immunomodulator. This study aims to determine the effect of C. papaya extract to the diameter size alba pulp of the spleen of Ovalbumin (OVA) induced Balb/C mice. The research design was experimental in vivo animal test with post-test only control group design. Thirty male Balb/C mice, ±20grams weight, 8 weeks old, divided into six groups consists of a normal control, negative control, Methylprednisolone, and C. papaya extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day). The extract and Methylprednisolone are given for 28 days, while OVA is given on 15thand 22ndday (intraperitoneal,) and 23rd until 28thday (peroral). On the 29thday the mice are sacrificed and the duodenum was made into histological preparation with HE staining. The immunomodulator effect is assessed by the average of the diameter size alba pulp of the spleen under a light microscope. The data were analyzed using SPSS15 with one way ANOVA continued with Tukey test. The average of the diameter size alba pulp of the spleen for control group is 10,165 ± 0,195; negative control group is 30,357 ± 0,826; methylprednisolone group is 10,220 ± 0,346; 175mg/kgbb dose extract is 14,956 ± 1,057; 350mg/kgbb dose extract is 14,030 ± 0,667; and 700mg/kgbb dose extract is 12,504 ± 0,729. The conclusion of this study is Carica papaya L. extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day) has an effect to the average of the diameter size alba pulp of the spleen in OVA-induced Balb/C mice significantly. 700mg/kg dose per day has the same effect with the methylprednisolone group. Keywords: immunomodulator, Carica papaya L., alba pulp of the spleen, ovalbumin

scholarly journals Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Zuraida Zuraida ◽  
Eti Yerizel ◽  
Eliza Anas
Keyword(s):  
Vitamin C ◽  
Negative Control Group ◽  
Organ Damage ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstrak Pemberian rosella (Hibiscus sabdariffa Linn) diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4). Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA). Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn) terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4), perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb) dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb). Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05). Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn) is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn) on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr. Samples were taken at random and divided into 4 groups consisting of a negative control group, positive control (CCl4), treatment 1 (CCl4 and roselle extract 250 mg / kg bw) and treatment 2 (CCl4 and roselle extract 500 mg / kg bw). CCl4 was given a single dose orally, after 24 hours, the subjects were given rosella extract orally for 14 days. Data were analyzed by ANOVA with a confidence level of 95%. Rosella extract obtained statistically significant differences of MDA and catalase levels among groups (p <0.05). It can be concluded that the rosella extract can reduce levels of MDA and increase the activity of catalase mice exposed to CCl4. From the research it can be concluded that the rosella extract can reduce levels of MDA and increas the activity of catalase mice exposed to CCl4. It is needed further research on the toxicity of extracts of rosella and organ damage caused.Keywords: carbon tetrachloride, MDA, catalase, rosella

scholarly journals Potential of Cocoa Ethanol Extract Toward Nematocyst Tubul Firing Inhibition of Physalia Utriculus Toxin In Vitro

2019 ◽  
Vol 5 (2) ◽  
pp. 30
Author(s):  
Sarwendah Siswi Winasis ◽  
Al Munawir ◽  
Adelia Handoko
Keyword(s):  
Normal Control ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Average Percentage ◽  
Post Test

There was estimated 150 million envenomation cases due to jellyfish stings occur globally every year. 100 from 10,000 jellyfish species in the world known to be dangerous, one them is Physalia utriculus. The aim of this study was to determine the potential of cocoa (Theobroma cacao L.) ethanol extract toward nematocyst tubul firing inhibition of jellyfish (Physalia utriculus) toxin in vitro. The method was true experimental design with post test only control group design. The study divided into 8 groups: 1 normal control, 1 positive control, 1 negative control, and 5 treatments grups by giving cocoa ethanol extract with concentration 20%, 2%, 0.2%, 0.02%, 0.002%. The observation was made by calculating the percentage number of firing nematocysts. The result showed average percentage of firing nematocyst in the normal control group was 42.50 ± 3.18, positive control group was 37.97 ± 5.57, negative control group was 52.44 ± 2.98, and treatment group which given with cocoa ethanol extract 20%, 2%, 0.2%, 0.02%, 0.002% were 48.24 ± 5.37; 40.62 ± 7.10; 29.45 ± 5.39; 37.60 ± 9.78; 41.11 ± 3.92, respectively. The One Way Annova statistical results test show significance value 0.001 (p≤0.05). The conclusion of this study was the cocoa ethanol extract with concentration 0.2% has most potential to inhibit the jellyfish (P. utriculus) nematocyst tubule firing in vitro. Keywords: Physalia utriculus, cocoa, toxin, jellyfish

scholarly journals Role Of Moringa Oleifera Leaf Extract In Increasing Hemoglobin Levels In Pregnant Rats With Anemia

2021 ◽  
Vol 14 (1) ◽  
pp. 8-13
Author(s):  
Reny Retnaningsih ◽  
Rani Safitri
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Control Group ◽  
Control Group Design ◽  
Pregnant Rats ◽  
Nutritional Problem ◽  
Post Test

Anemia is one of the epidemic health problems in society and the most common nutritional problem. The Moringa oleifera is rich in excellent dietary content and can complement the body's needs. This study aims to determine Moringa oilefera leaf extract's effectiveness in increasing hemoglobin levels in pregnant rats (Ratus norvegicus) with anemia. This paper used a true experimental research design with a pre-post test randomized control group design. This study utilized female rats (Ratus norvegicus), pregnant 8-12 weeks, weighing 200 grams. Experimental procedures were four treatments with three repetitions. Types of treatment were negative control and positive control (administration of Moringa oilefera leaf extract) at a dose of 0.18 g / head, 0.36 g / head, and 0.72 g/head. Analysis using one way ANOVA with an error rate of 0.05. The results showed p<0.005, which means a significant increase in hemoglobin levels in the group given the treatment of moringa leaf extract at a dose of 0.72 g/head/day. The conclusion and suggestion from this research are public could get information about using natural materials as economically valuable drugs (back to nature)

scholarly journals Effect of Chitosan on Histology of Reproductive Organs of Female Wistar Rats (Rattus norvegicus) Exposed to Acetate Lead

2019 ◽  
Vol 30 (4) ◽  
pp. 259
Author(s):  
Alief Ayu Purwitasari ◽  
Annisa' Wigati Rozifa ◽  
Dwi Dianita Irawan ◽  
Umi Kalsum ◽  
Retty Ratnawati ◽  
...  
Keyword(s):  
Treatment Group ◽  
Lead Acetate ◽  
Antral Follicle ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Female Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design

Lead is one of the pollutants widely spread in the environment because it is not easily decomposed. Lead can affect system functions such as the ovary and endometrium. Lead can trigger oxidative stress by reducing antioxidant enzymes and increasing Reactive Oxygen Species (ROS). Lead can also reduce Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels by disturbing the hypothalamus. Chitosan is an antioxidant compound that can reduce the toxic effects of lead. The purpose of this study was to study the effects of chitosan administration on the diameter of antral follicles, the number of endometrial arterioles, and the thickness of endometrial rats after lead acetate exposure. This study was an experimental laboratory using a posttest-only control group design approach applied on 25 female rats aged 8 weeks old, body weight 125-175 grams. Lead and chitosan were given orally with a sonde. There were 5 groups, namely, negative control group (without any treatment), positive control group (lead 175mg/kg/BW), treatment group 1 (lead 175mg/kg/BW + chitosan 16mg/kg/BW, treatment group 2 (lead 175mg/kg/BW + chitosan 32mg/kg/BW), and treatment group 3 (lead 175mg/kg/BW + chitosan 64mg/kg/BW) for 30 days. The rats were sacrificed at proestrus phase, which was proven from vaginal swab. Observations were carried out using the Hematoxylin Eosin (HE) staining method. The observations were analyzed using One Way ANOVA and followed by Least Significant Differences (LSD) test. The results showed significant results (p-value &lt;0.05). Chitosan can increase the diameter of the antral follicle, increase the number of endometrial arterioles, and increase the thickness of endometrial rats exposed by lead acetate.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals An IGF-1 Levels Decrease in SOPK-RI Model Mice After A Low-Carb Diet

2020 ◽  
Vol 9 (2) ◽  
pp. 325-331
Author(s):  
Ermawati Ermawati ◽  
Budi Santoso ◽  
Widjiati Widjiati ◽  
Hany Puspita Aryani
Keyword(s):  
Design Research ◽  
Polycystic Ovary ◽  
Negative Control Group ◽  
Positive Control ◽  
Vaginal Swab ◽  
Negative Control ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Control Group Design

The aim of this study is to analyze the IGF-1 levels decrease in mice modeled by Polycystic Ovary Syndrome Insulin Resistance post carbohydrate high in protein. True Experimental Design with a Posttest Control Group Design research using 36 tails, female Wistar Rattus Norvegicus Strains, 3 months, 100-200 grams and randomly divided into 3 groups, namely K-(n =12, negative control group), K+(n=12, positive control group) and KP(n=12, treatment group). SOPK-IR modeling is done by mice in the injection of the hormone testosterone provionate at a dose of 100 mg/kg BW for 28 days, on the last day after injection a vaginal swab is performed to identify the condition of diestrus. The measurement of IGF-1 levels used the Enzyme-Linked Immunosorbent Assay method. Data analysis techniques used ANOVA test and LSD post hoc test. The results obtained mean levels of IGF-1 at K- (234.126±32.568) ng/mL, K+ (169.662±30.150) ng/mL, KP (146.528±29.562) ng/mL (p=0.000)

scholarly journals Effectiveness of Boiled Comfrey Leaves (Symphytum Officinale L) on SGOT SGPT Serum in Male Wistar Strain Rats With Acute Hepatitis Models

2019 ◽  
Vol 7 (1) ◽  
pp. 756-767
Author(s):  
Rois Sahidin ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Symphytum Officinale ◽  
Wistar Strain

Acute hepatitis is an acute disease caused by viruses, alcohol and drugs, which is characterized by an increase in SGOT SGPT serum. The purpose of this study was to determine the effectiveness of boiled comfrey leaves (Symphytum Officinale L) levels of SGOT and SGPT serum in male wistar strain rats with acute hepatitis models. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. The objects were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 2.8 grams of comfrey (symphytum officinale L) leaves was given as much as 2.7 cc orally for 7 days to the treatment group. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare serum levels of SGOT & SGPT. The results showed there were significant differences in serum levels of SGOT between the treatment group, positive control and negative control (p <0.05) and there were significant differences in SGPT results between the treatment group and positive control group (p <0.05) but there were no significant differences between the SGPT levels of the treatment group and the negative control group (p = 0.173). As Conclusion, boiled Comfrey leaf has an effect in decreasing serum SGOT & SGPT in male wistar strain rats with acute hepatitis model.

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