scholarly journals PEMANFAATAN Baringtonia asiatica DAN Annona muricata TERHADAP SERANGGA VEKTOR PENYAKIT PADA TANAMAN CABAI

EUGENIA ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Christina L. Salaki ◽  
Jantje Pelealu
Keyword(s):  
Crop Production ◽  
Aphis Gossypii ◽  
Seed Extract ◽  
Insect Population ◽  
Botanical Insecticides ◽  
Insect Vector ◽  
Insect Vectors ◽  
Annona Muricata ◽  
Green House ◽  
Lethal Level

ABSTRACT   Insect vectors disease is a major problem for chilli crop production in North Sulawesi. The effect is significant in decreasing crop production. The use of plant-derived insecticide is potential and environmentally friendly way in controlling the insect, as a form of integrated pest management. Seeds of Bitung (Barringtonia asiatica) and seeds of soursop (Annona muricata) plants can be used as an organic insectiside to control the several types of insect vectors  in pepper crop. The purpose of this study was to determine effects of the application of extracts of B. asiatica and A. muricata on the growth of insect vector (Aphis gossypii) in pepper and and determine the extracts’s lethal level.  This research was conducted in the Green House of Plant Pests and Diseases Department of the Faculty of Agriculture Unsrat Manado, for 6 months time. The research used a complete randomized design consisting of 6 treatments;  each treatment was consisted of three replications. The results showed that the percentage of dead insects assay (Aphis gossypii) increased as the concentration of the extract used increased. This study found that the concentration of soursop seed extract at 50 g / l and a concentration of 1.75% Bitung seed extract was the best to be used as a botanical insecticide in controlling the insect population vector (Aphis gossypii) in laboratory conditions. It has an effective lethal concentration for 93, 67% and 90.0% of all test insect population. Overall bioactivity increased the effectiveness of soursop Bitung seed extract as botanical insecticides. Keywords: Barringtonia asiatica, Annona muricata, Aphis gossypii, chilli plants ABSTRAK   Serangga vektor penyakit merupakan masalah utama bagi produksi tanaman cabai di Sulawesi Utara karena dapat menurunkan produksi. Salah satu sarana pengendalian yang memiliki peluang baik untuk dikembangkan dalam menunjang penerapan PHT adalah pemanfaatan bahan insektisida yang berasal tumbuhan. Buah bitung (Barringtonia asiatica) dan biji sirsak (Annona muricata) adalah jenis tumbuhan yang berpotensi untuk dikembangkan dan efektif terhadap beberapa jenis serangga vektor pada tanaman cabai. Tujuan penelitian ini untuk mengetahui pengaruh ekstrak B. asiatica dan A. muricata terhadap perkembangan dan daya bunuh terhadap serangga vektor (Aphis gossypii) pada tanaman cabai. Penelitian ini dilaksanakan di Green House Jurusan Hama dan Penyakit Tumbuhan Fakultas Pertanian Unsrat Manado, selama kurang lebih 6 bulan. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap yang terdiri dari 6 perlakuan dan tiap perlakuan terdiri dari 3 ulangan. Hasil penelitian menunjukkan bahwa persentase kematian serangga uji (Aphis gossypii) meningkat seiring dengan meningkatnya konsentrasi ekstrak yang digunakan. Dalam penelitian ini didapati bahwa konsentrasi ekstrak biji sirsak sebesar 50 g/l dan konsentrasi ekstrak biji bitung 1,75 % sangat baik digunakan sebagai insektisida botani dalam mengendalikan populasi serangga vektor (Aphis gossypii) pada kondisi laboratorium, karena konsentrasi ini efektif mematikan sebesar 93,67 % dan 90,0 % seluruh populasi serangga uji. Keseluruhan bioaktivitas tersebut menambah keefektifan ekstrak biji sirsak dan buah bitung sebagai insektisida botani.  Eugenia Volume 18 No. 1  April 2012 Kata Kunci : Barringtonia asiatica, Annona muricata, Aphis gossypii, tanaman cabai

scholarly journals Bacteriophage-Host Association in the Phytoplasma Insect Vector Euscelidius variegatus

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 612
Author(s):  
Marta Vallino ◽  
Marika Rossi ◽  
Sara Ottati ◽  
Gabriele Martino ◽  
Luciana Galetto ◽  
...  
Keyword(s):  
Crop Production ◽  
Axenic Culture ◽  
Pathogen Transmission ◽  
Economic Losses ◽  
Insect Vector ◽  
Insect Vectors ◽  
Electron Microscopic ◽  
Flavescence Dorée ◽  
Euscelidius Variegatus ◽  
Complex Relationships

Insect vectors transmit viruses and bacteria that can cause severe diseases in plants and economic losses due to a decrease in crop production. Insect vectors, like all other organisms, are colonized by a community of various microorganisms, which can influence their physiology, ecology, evolution, and also their competence as vectors. The important ecological meaning of bacteriophages in various ecosystems and their role in microbial communities has emerged in the past decade. However, only a few phages have been described so far in insect microbiomes. The leafhopper Euscelidius variegatus is a laboratory vector of the phytoplasma causing Flavescence dorée, a severe grapevine disease that threatens viticulture in Europe. Here, the presence of a temperate bacteriophage in E. variegatus (named Euscelidius variegatus phage 1, EVP-1) was revealed through both insect transcriptome analyses and electron microscopic observations. The bacterial host was isolated in axenic culture and identified as the bacterial endosymbiont of E. variegatus (BEV), recently assigned to the genus Candidatus Symbiopectobacterium. BEV harbors multiple prophages that become active in culture, suggesting that different environments can trigger different mechanisms, finely regulating the interactions among phages. Understanding the complex relationships within insect vector microbiomes may help in revealing possible microbe influences on pathogen transmission, and it is a crucial step toward innovative sustainable strategies for disease management in agriculture.

scholarly journals IMPACT OF BOTANICAL INSECTICIDES DERIVED FROM Pangium edule Reinw AND Annona muricata L. SEED EXTRACTS ON THE “GAY GANTUNG” DIAMONDBACK MOTH, Plutella xylostella L.

2017 ◽  
Vol 2 (2) ◽  
pp. 27-35
Author(s):  
Ernest Hanny Sakul
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Probit Analysis ◽  
Seed Extract ◽  
Hexane Fraction ◽  
Botanical Insecticides ◽  
Annona Muricata ◽  
Active Components ◽  
Botanical Pesticides ◽  
Randomized Design

Insecticidal properties of fractioned extracts from Pangium edule Reinw seed and Annona muricata seed against Plutella xylostella larvae were investigated in the laboratory. The study was initiated to investigate the possibility of using botanical pesticides to control P.xylostella, a serious cosmopolitan pest of crucifer plants. The study aims to determine the most effective concentration and the most active extract;  to evaluate the different extract concentrations on the treated larvae; and to characterize the phytochemical contents of the most effective extracts fraction. The study was an experiment initiated by test of phytochemical screening test in order to discover the presence of secondary metabolites in the extracts. It was followed by the test of mortality of the diamondback moth larvae. Furthermore, the extracts hexane fraction and etanol fraction, were conducted with completely randomized design; The LC50 values were determined following probit analysis, the data were treated in the software programme IBM Statistic SPSS 20. Results showed that n-hexane fraction is the most effective againts larvae (LC50-48h = 12,71 mg/L) from P.edule seed extract, (LC50-48h = 50,81 mg/L) from A.muricata seed extract. Larva mortality was highest using 1000 ppm n-hexane fraction (96,6%) derived from P.edule seed extract, (93,3%) derived from A.muricata seed extract. The ethanol fraction tested positive for alkaloid, saponins, flavonoids, terpenoids, phenol and tannins. N-hexane fraction of P.edule seed extract, and A.muricata seed extract are an effective botanical insecticides exhibiting larvicidal and antifeedant properties against P.xylostella thus it can be alternative to synthethic insecticides. Results indicate that these botanical insecticides have good possibilities for control of P.xylostella. Further work is necessary to evaluate and characterize the active components of the extract fractions and its efficacy in the fieldInsecticidal properties of fractioned extracts from Pangium edule Reinw seed and Annona muricata seed against Plutella xylostella larvae were investigated in the laboratory. The study was initiated to investigate the possibility of using botanical pesticides to control P.xylostella, a serious cosmopolitan pest of crucifer plants. The study aims to determine the most effective concentration and the most active extract;  to evaluate the different extract concentrations on the treated larvae; and to characterize the phytochemical contents of the most effective extracts fraction. The study was an experiment initiated by test of phytochemical screening test in order to discover the presence of secondary metabolites in the extracts. It was followed by the test of mortality of the diamondback moth larvae. Furthermore, the extracts hexane fraction and etanol fraction, were conducted with completely randomized design; The LC50 values were determined following probit analysis, the data were treated in the software programme IBM Statistic SPSS 20. Results showed that n-hexane fraction is the most effective againts larvae (LC50-48h = 12,71 mg/L) from P.edule seed extract, (LC50-48h = 50,81 mg/L) from A.muricata seed extract. Larva mortality was highest using 1000 ppm n-hexane fraction (96,6%) derived from P.edule seed extract, (93,3%) derived from A.muricata seed extract. The ethanol fraction tested positive for alkaloid, saponins, flavonoids, terpenoids, phenol and tannins. N-hexane fraction of P.edule seed extract, and A.muricata seed extract are an effective botanical insecticides exhibiting larvicidal and antifeedant properties against P.xylostella thus it can be alternative to synthethic insecticides. Results indicate that these botanical insecticides have good possibilities for control of P.xylostella. Further work is necessary to evaluate and characterize the active components of the extract fractions and its efficacy in the field

scholarly journals Bioaktivitas ekstrak biji bintaro terhadap kutu daun Aphis gossypii GLOVER dan pengaruhnya terhadap tanaman cabai

Jurnal Agro ◽  
10.15575/8380 ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 179-192
Author(s):  
Rial Mustiarif ◽  
Djamilah Djamilah ◽  
Nanik Setyowati ◽  
Agustin Zakarni
Keyword(s):  
Insect Pest ◽  
Aphis Gossypii ◽  
Dry Weight ◽  
Seed Extract ◽  
Crop Damage ◽  
Time Of Application ◽  
Randomized Design ◽  
Aphis Gossypii Glover ◽  
Before And After ◽  
Two Factors

Aphis gossypii Glover hama penting tanaman cabai, dapat dikendalian dengan menggunakan pestisida nabati. Penelitian bertujuan untuk mengevaluasi waktu aplikasi dan konsentrasi ekstrak kasar biji bintaro (Cerbera odollam G.) dalam mengendalikan A. gossypii Glover serta pengaruhnya terhadap pertumbuhan tanaman cabai. Penelitian menggunakan Rancangan Acak Lengkap, dua faktor dan tiga ulangan. Faktor pertama adalah waktu aplikasi ekstrak (waktu sebelum dan waktu setelah infestasi A. gossypii Glover). Faktor kedua adalah konsentrasi ekstrak bintaro (0%, 1%, 2%, 3%, 4% dan 5%). Hasil penelitian menunjukkan konsentrasi 1% ekstrak biji bintaro menyebabkan mortalitas kutu daunnya 68% sedangkan pada konsentrasi 3% mortalitasnya mencapai 90%. Ekstrak biji bintaro yang diberikan sebelum hama diinfestasikan menyebabkan mortalitas kutu daun 59,5% sedangkan jika diberikan setelah hama diinfestasikan mortalitasnya meningkat menjadi 77,6%. Nilai LC50 dan LC90 ekstrak biji bintaro diaplikasikan sebelum dan setelah hama diinfestasikan secara berurutan adalah 1,8%; 4,4%; 0,57% dan 2,8%. A. gossypii Glover yang diinfestasikan pada tanaman berumur 4 minggu dan disemprot dengan ekstrak biji bintaro pada konsentrasi 1% dapat menurunkan intensitas kerusakan pada hari ke tujuh setelah infestasi dan tidak berpengaruh terhadap jumlah daun, bobot segar maupun bobot kering tanaman cabai. Ekstrak biji bintaro dapat dikembangkan sebagai pestisida nabati untuk mengendalikan hama A. gossypii Glover pada tanaman cabai. Aphis gossypii Glover is an important sucking insect pest of the pepper (Capsicum annum L.), Can be controlled by using natural pesticides. The study aimed to evaluate the application time and concentration of Cerbera odollam G. seed extract in controlling A. gossypii and their effects on pepper, C. annum. Complete randomized design was used in this experiment, with two factors, and was repeated three times. The first factor was the time of application of the extract, consisting of before and after A. gossypii infestation. The second factor was the concentration of C. odollam extract (0%, 1%, 2%, 3%, 4% and 5%). The results indicated the C. odollam seed extract affected the mortality of A. gossypii. At a concentration of 1% of C. odollam seed extract, the mortality of A. gossypii was 68% while at a concentration of 3% the mortality reached up to 90%. C. odollam seed extract applied before infestation, the mortality of A. gossypii was 59.5% whereas, after the infestation, the mortality increased up to 77.6%. LC50 and LC90 extract of C. odollam seeds applied before and after infestation were 1.8%; 4.4%; 0.57% and 2.8% respectively. A. gossypii infested at 8 weeks old pepper and sprayed with C. odollam seed extract at a concentration of 1% lowered the crop damage on the seventh day after infestation. On the other hand, the application of C. odollam seed extract did not affect the number of leaves, fresh weight, and dry weight of pepper. This study indicate that C. odollam seed extract can be developed as a natural pesticide to control A. gossypii on pepper..

scholarly journals Efficacy of Two Monoterpenoids, Carvacrol and Thymol, and Their Combinations against Eggs and Larvae of the West Nile Vector Culex pipiens

Molecules ◽  
2019 ◽  
Vol 24 (10) ◽  
pp. 1867 ◽  
Author(s):  
Mohammad Reza Youssefi ◽  
Mohaddeseh Abouhosseini Tabari ◽  
Aryan Esfandiari ◽  
Sohrab Kazemi ◽  
Ali Akbar Moghadamnia ◽  
...  
Keyword(s):  
Culex Pipiens ◽  
Mosquito Control ◽  
Fixed Ratio ◽  
Botanical Insecticides ◽  
Ratio Method ◽  
Insect Vector ◽  
The West ◽  
West Nile ◽  
Lc50 Value ◽  
Insecticidal Efficacy

Background: Insect vector control is facing the challenges of resistance development and environmental hazards caused by synthetic pesticides. This has led to a considerable market opportunity for botanical insecticides. In this scenario, our study investigated the potential of selected bioactive monoterpenoids, carvacrol and thymol, as safe and effective tools to control the West Nile vector Culex pipiens. Furthermore, the combined effect of thymol-carvacrol mixtures and their possible interactions were assessed. Methods: For determining larvicidal and ovicidal 50% lethal concentration (LC50), each monoterpenoid was tested at different concentrations (5–500 mg/L). Then, the fixed ratio method was used for evaluating their combinational efficacy. Results: Carvacrol was more toxic against larvae of Cx. pipiens, with a LC50 value of 14 mg/L, whereas thymol exhibited a LC50 value of 49 mg/L. Comparable trends of efficacy were observed when toxicity on Cx. pipiens eggs was investigated, with LC50 values of 7 and 13 mg/L for carvacrol and thymol, respectively. In combinational toxicity assays, the mixture thymol-carvacrol at 1:4 ratio achieved a synergistic effect against larvae of Cx. pipiens, whereas an additive effect was observed on eggs. Other ratios showed antagonistic effects. Conclusions: Overall, our findings pointed out that the 1:4 ratio of thymol-carvacrol blend can enhance the insecticidal efficacy on Cx. pipiens young instars and can be considered further as active ingredient for developing botanical insecticides to be used in mosquito control operations.

World Management of Geminiviruses

2018 ◽  
Vol 56 (1) ◽  
pp. 637-677 ◽  
Author(s):  
Maria R. Rojas ◽  
Monica A. Macedo ◽  
Minor R. Maliano ◽  
Maria Soto-Aguilar ◽  
Juliana O. Souza ◽  
...  
Keyword(s):  
Crop Production ◽  
Insect Vector ◽  
Health Issues ◽  
Annual Crops ◽  
Row Covers ◽  
Vector Management ◽  
Management Application ◽  
Future Challenges ◽  
Agriculture Management ◽  
Open Fields

Management of geminiviruses is a worldwide challenge because of the widespread distribution of economically important diseases caused by these viruses. Regardless of the type of agriculture, management is most effective with an integrated pest management (IPM) approach that involves measures before, during, and after the growing season. This includes starting with resistant cultivars and virus- and vector-free transplants and propagative plants. For high value vegetables, protected culture (e.g., greenhouses and screenhouses) allows for effective management but is limited owing to high cost. Protection of young plants in open fields is provided by row covers, but other measures are typically required. Measures that are used for crops in open fields include roguing infected plants and insect vector management. Application of insecticide to manage vectors (whiteflies and leafhoppers) is the most widely used measure but can cause undesirable environmental and human health issues. For annual crops, these measures can be more effective when combined with host-free periods of two to three months. Finally, given the great diversity of the viruses, their insect vectors, and the crops affected, IPM approaches need to be based on the biology and ecology of the virus and vector and the crop production system. Here, we present the general measures that can be used in an IPM program for geminivirus diseases, specific case studies, and future challenges.

scholarly journals Efficacy of Citrullus colocynthis seed extract on Earias vittella, Fabricius, (Lepidoptera: Noctuidae): environment sustainable approach

2024 ◽  
Vol 84 ◽  
Author(s):  
U. A. Hassam ◽  
A. Gulzar ◽  
B. Rasool ◽  
S. Zafar ◽  
T. Younis ◽  
...  
Keyword(s):  
Esterase Activity ◽  
Seed Extract ◽  
Control Treatment ◽  
Intrinsic Rate Of Increase ◽  
Botanical Insecticides ◽  
Earias Vittella ◽  
Citrullus Colocynthis ◽  
Net Reproductive Rate ◽  
Insect Management ◽  
Rate Of Increase

Abstract Earias vittellaFabricius, 1794 (Noctuidae: Lepidoptera) is deliberated to be one of the most destructive pests of cotton and okra vegetation in the world including Asia. The pest has established resistance to various synthetic insecticides. The use of bio-pesticide is one of the unconventional approaches to develop a vigorous ecosystem without harming non- target pests and beneficial natural insect fauna. In the present study, the toxicity levels of Citrullus colocynthis seed extract have been evaluated against the populations of E. vittellaunder standardized laboratory conditions. The toxic effects of C. colocynthis on development periods, protein contents and esterase activity of the life stages of E. vittella were also evaluated. The toxicity levels of methanol, ethanol, hexane, water and profenofos were evaluated on the 1st instar larvae of E. vittella. LC30 and LC80 concentrations exhibited the effectiveness of methanol-based C. colocynthis seed extract against 1st instar larvae of E. vitella. The enhanced larval and pupal periods were revealed in treated samples during the comparison with untreated samples. The intrinsic rate of increase, net reproductive rate in the LC30 and LC80 concentrations exposed larvae remained less than the control treatment. Fecundity, the esterase activity and protein contents were declined in LC30 and LC80 treated samples as compared to the control. The present findings suggest that C. colosynthis extracts based botanical insecticides are beneficial, ecosystem sustainable and can be integrated with insect management programs from environment safety perspective.

scholarly journals Larvicidal and Enzymatic Inhibition Effects of Annona muricata Seed Extract and Main Constituent Annonacin against Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

2019 ◽  
Vol 12 (3) ◽  
pp. 112 ◽  
Author(s):  
Rodrigues ◽  
Silva ◽  
Pinto ◽  
Lima dos Santos ◽  
Carneiro de Freitas ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Bioactive Compounds ◽  
Larvicidal Activity ◽  
Aedes Albopictus ◽  
New Products ◽  
Seed Extract ◽  
Annona Muricata ◽  
Main Constituent ◽  
Environmentally Sustainable ◽  
Lc50 And Lc90

The mosquitoes Aedes aegypti and Aedes albopictus are vectors of arboviruses that cause dengue, zika and chikungunya. Bioactive compounds from plants are environmentally sustainable alternatives to control these vectors and thus the arboviruses transmitted by them. The present study evaluated the larvicidal activity of an acetogenin-rich fraction (ACERF) and its main constituent annonacin obtained from Annona muricata seeds on Ae. aegypti and Ae. albopictus. The larvicidal assays were performed using different concentrations to calculate the LC50 and LC90 values observed 24 h after exposure to the treatment. Annonacin was more active against Ae. aegypti (LC50 2.65 μg·mL−1) in comparison with Ae. albopictus (LC50 8.34 μg·mL−1). In contrast, the acetogenin-rich fraction was more active against Ae. albopictus (LC50 3.41 μg·mL−1) than Ae. aegypti (LC50 12.41 μg·mL−1). ACERF and annonacin treated larvae of Ae. aegypti and Ae. albopictus showed significant differences in the inhibition of their metabolic enzymes when compared to untreated larvae. The results demonstrate the relevant larvicidal action of the acetogenin-rich fraction and annonacin showing the potential to develop new products for the control of Ae. aegypti and Ae. albopictus.

scholarly journals Evaluation of Some Botanical Extracts Against Major Insect Pests (Leafminer, Armored scale and Woolly Whitefly) of Citrus Plants in Central Zone of Tigray, North Ethiopia

2020 ◽  
Vol 11 (2) ◽  
pp. 258-275
Author(s):  
Fitiwy Ibrahim ◽  
Tsehaye Hadush ◽  
Gebretsadkan Abraha ◽  
Araya Alemu
Keyword(s):  
Insect Pests ◽  
Block Design ◽  
Central Zone ◽  
Seed Extract ◽  
Scale Insects ◽  
Limiting Factors ◽  
Botanical Insecticides ◽  
Neem Seed ◽  
Chemical Insecticide ◽  
Neem Seed Extract

Citrus fruit production is suffering from various yield-limiting factors particularly the sucking pests viz, citrus leafminer Phyllocnistis citrella, woolly whitefly, Aleurothrixus floccosus and scale insects especially armored scales Aonidiella auriantii. The present paper tries to identify the effective botanical insecticides against these insect pests on citrus orange. An experiment was conducted in the established citrus orange farm in Kolla Temben at two farmer’s fields, Adiha and Agibe during the off-season of 2018 under irrigation growing condition in a randomized complete block design with six treatments and replicated thrice. The findings in both experimental sites showed that the neem seed extract had a significantly (P=0.001) lower leafminer infestation levels compared to the untreated control which is on par to the insecticide treatment of dimethoate. On the other hand, in both areas, whitefly mortality of more than 81% on average was recorded from neem seed extract followed by Tree tobacco (70%) (P=0.001). For scale insects however, in both experimental sites, the highest mean percent mortality was recorded from the insecticide dimethoate 40% EC (86.9%, 87.2 & 86.0%on average) followed by neem seed and tree tobacco extracts,70.1 and 65.4% respectively. The botanicals particularly the neem seed extracts followed by tree tobacco are as effective as the chemical insecticide, dimethoate 40% EC, even superior in some cases, in controlling the target pests. Therefore, these botanicals could be used as an IPM component for against the target pests.

scholarly journals THE POTENCY OF BOTANICAL INSECTICIDES TO CONTROL Aphis gossypii (HEMIPTERA:COCCOIDEA:APHIDIDAE) ON PATCHOULI PLANT

2016 ◽  
Vol 25 (1) ◽  
pp. 61
Author(s):  
Tri Lestari Mardiningsih ◽  
Nurbetti Tarigan ◽  
Cucu Sukmana ◽  
Agus Kardinan
Keyword(s):  
Observation Interval ◽  
Block Design ◽  
Aphis Gossypii ◽  
Botanical Insecticides ◽  
Research Station ◽  
Clove Oil ◽  
Neem Oil ◽  
Neem Extract ◽  
Randomized Block Design ◽  
Medicinal Crops

<p class="IsiabstrakIndonesia">Attack of <em>Aphis gossypii</em> is one of constraints in cultivating of patchouli plant. An experiment with the purpose to examine the effectiveness of botanical insecticides to <em>A. gossypii </em>on patchouli plant. The experiment was carried out in Research Station of Indonesian Spice and Medicinal Crops Research Institute, at Cicurug, Sukabumi from June to October 2011. The experiment was arranged in a Randomized Block Design, nine treatments and three replications. The treatments were mixing of neem oil + soap nuts, neem oil + clove oil, neem oil + citronellal oil, neem oil + clove oil + citronellal oil, citronellal oil, clove oil, neem extract, carbosulfan, and control. Patchouli plants were planted with spacing of 40 cm x 60 cm. The number of plant population plot<sup>-1</sup> was 25 plants and the number of sample plot<sup>-1</sup> for observation was five plants. Sampling of observed plants was conducted diagonally. Observation was carried out by counting population of <em>A. gossypii</em> on one shoot. Application of insecticides was conducted one day after the first observation. Interval of application was every week, conducted eight times. Other parameters observed were plant height and production of patchouli. Results showed that all treatments of botanical insecticides were effective to decrease of <em>A. gossypii</em><em> </em>population. The most effective treatment was that of neem extract with the value of  efficacy was more than 80% occurred at seven assessments. Plant high between treatment is not significant different.</p>

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