scholarly journals Isolation and Characterization of Hermetia Illucens Larval Protein for the Assessment of Inhibitory Activity against MCF7 and HeLa cell Lines

2020 ◽  
Vol 9 (3) ◽  
pp. 3045-3050
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Hela Cells ◽  
Inhibitory Activity ◽  
Crude Protein ◽  
The Body ◽  
Assay Method ◽  
Hermetia Illucens ◽  
Isolation And Characterization ◽  
Hela Cell Lines

Cancer has a major impact on society having the highest morbidity and mortality rate. Approximately 70% of deaths are predominantly caused due to cancer and it prevails in low and middle-income countries, as they are unaware of the signs and symptoms, treatment methods, non-availability of cancer drugs and the incurrence of huge economic burden on the family. As per the literature, the chemotherapeutic drug has numerous side effects and occasionally it may lead to death. Based on these inferences, there is a requirement to identify therapeutic peptides that are more effective for cancer treatment with less toxic effects on the body. Peptides have a great advantage to fight against the disease efficiently. Several flies have anticancer, antibacterial, antifungal and antioxidant properties that help to control disease and inhibit cancer progression. In the present research, we have utilized Hermetia illucens has great anticancer peptides that can control cancer growth. In the current research, we utilized crude protein extraction from Hermetia illucens larvae, to discover the inhibitory activity against MCF7 and HeLa cell lines. by adopting the MTT assay method, along with reference drug Camptothecin. Bradford assay results helped to identify that 23.5µg/ml concentration of protein is present in the crude sample. Flow cytometry was used to calculate the % of cell violations and IC50 values were observed in HeLa (19.88±50.3μg/mL) and MCF7 (224.68±50.3μg/mL) cells. Cell cycle inhibition was observed in HeLa cells of G1 (57.3±1.2) and, S (19.8±1.3) phase of dose-dependent with the significance of (P<0.05). Hermetia illucens crude protein has significant inhibition with HeLa cells that have the best anticancer proteins in human cervical carcinoma cells.

scholarly journals Antiproliferative effects of some medicinal plants on HeLa cells

2013 ◽  
Vol 65 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Desanka Cenic-Milosevic ◽  
Z. Tambur ◽  
D. Bokonjic ◽  
S. Ivancajic ◽  
Tatjana Stanojkovic ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Hela Cells ◽  
Melissa Officinalis ◽  
Echinacea Angustifolia ◽  
Antiproliferative Effects ◽  
Hela Cell Lines ◽  
Ic50 Value

Medicinal plants maintain the health and vitality of individuals, and also have potential curative effect on various diseases, including cancer. In this study were investigated the antiproliferative effects of water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Echinacea angustifolia, Salvia officinalis and Melissa officinalis) on cell lines derived from human cervix adenocarcinoma (HeLa cells). The best cytotoxic activity (IC50 = 43.52 ?g/ml) on HeLa cell lines was exhibited by Echinacea angustifolia. The extract of Salvia officinalis also showed a good cytotoxic activity against HeLa cell lines; the IC50 value was 70.41 ?g/ml. Melissa officinalis manifested a slightly weaker cytotoxic activity and an IC50 value of 122.22 ?g/ml.

scholarly journals A Study on the Ethanolic Extracts of Red Seaweed- Gracilaria corticata (J.Agardh) J. Agardh, to Assess the Antiproliferative Activity and Morphological Characterization of Apoptosis on HeLa Cell lines

2017 ◽  
Vol 9 (3) ◽  
pp. 436 ◽  
Author(s):  
Ashwini S ◽  
Manjula Shantaram
Keyword(s):  
Hela Cell ◽  
Acridine Orange ◽  
Cell Lines ◽  
Hela Cells ◽  
Red Algae ◽  
Morphological Characterization ◽  
Gracilaria Corticata ◽  
Hela Cell Lines ◽  
Ethanolic Extracts ◽  
Morphological Assessment

<p>Marine algae are excellent source of bioactive compounds that can be used as an alternative source for finding novel anti-cancer molecules. Gracilaria corticata, red algae from Surathkal beach, Karnataka were studied for their anti-proliferative activities and their morphological characterization on HeLa cells were assessed. Cytotoxicity of the algal ethanolic extracts on HeLa cells were assayed by 3-(4,5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) reduction method. Morphological assessment was done by examinations with Hoechst staining and acid vacuoles were determined using acridine orange. Induction of apoptosis was studied using caspase activity. Based on IC<sub>50</sub>value, further morphological assessment was carried out and apoptosis was confirmed using Hoechst 33342 staining and acridine orange staining respectively. Treated cells became round with blebbings with condensed nuclei. Acidic lysosomal vacuoles formation occurred in treated cells. These red algae were able to suppress proliferation and promote apoptosis-- mediated cell death with induction of initial stages of apoptosis in HeLa cell lines. Thus, this seaweed can be a potent candidate for isolating new green drug anticancer molecules. However, further characterization at the molecular and structural levels are required.</p>

HGAL - A Germinal Center-Like Diffuse Large B-Cell Lymphoma Prognostic Biomarker Interacts with the Cytoskeleton and Influences Cell Migration.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 2022-2022
Author(s):  
Jun Chen ◽  
Feiying Ding ◽  
David M. Helfman ◽  
Izidore S. Lossos
Keyword(s):  
Cell Migration ◽  
Hela Cell ◽  
Tyrosine Phosphorylation ◽  
Cell Lines ◽  
Hela Cells ◽  
Germinal Center ◽  
B Cell Lymphoma ◽  
Lymphocyte Migration ◽  
Transfected Hela Cell ◽  
Hela Cell Lines

Abstract Gene expression profiling studies of lymphoid malignancies have led to the discovery of previously unrecognized lymphoma subtypes and associated novel genes. We recently cloned a germinal center (GC) specific gene - human germinal center-associated lymphoma (HGAL), whose expression correlates with survival in patients with diffuse large B-cell lymphoma (DLBCL) and classical Hodgkin lymphoma (HL). HGAL protein contains an ITAM motif and harbors 6 tyrosines that may be potentially phosphorylated. Mice deficient in the HGAL homologue M17 form normal GCs with the exception of reduced-sized Peyer’s patches, undergo efficient class-switch recombination and somatic hypermutation, and mount T-cell-dependent antibody responses similar to wild-type controls (Schenten et al., Blood 2006). The biological functions of HGAL in normal GC lymphocytes and lymphoma are unknown. Herein we demonstrate that HGAL plays a role in regulation of cell migration. Ex-vivo exposure of lymphoma or HGAL-transfected HeLa cell-lines to pervanadate resulted in tyrosine phosphorylation of HGAL protein. Stimulation of lymphoma and HGAL-transfected HeLa cell-lines with interleukin (IL)-6 led to transient HGAL tyrosine phosphorylation, peaking at 5–15 minutes. The phosphorylated HGAL had a markedly shortened life time compared to the unphosphorylated protein. No HGAL tyrosine phosphorylation was observed upon stimulation with anti IgM, interferon-g or IL-4. Deletion of the two C-terminal ITAM tyrosines in a truncated HGAL mutant (amino-acids 1-118) totally abrogated HGAL tyrosine phosphorylation upon IL-6 stimulation or exposure to pervanadate. Immunofluorescence and confocal miscroscopic studies of the SUDHL6 lymphoma and transfected HeLa cell-lines demonstrated HGAL redistribution from mainly perinuclear cytoplasmic localization to podosomes and spike-like filopodia. The time-frame of the HGAL relocalization was similar to that of HGAL tyrosine phosphorylation, being most prominent at 5 and 10 minutes in the SUDHL6 and transfected HeLa cells, respectively. Immunofluorescence studies demonstrated HGAL colocalization with actin, myosin and WASP proteins. HGAL immunoprecipitation studies followed by MAS spectroscopy or Western blotting confirmed HGAL interaction with actin and myosin type 2, but not with WASP protein. The N terminal portion of HGAL protein (aa 1-118) was sufficient for these interactions, however HGAL phosphorylation at its C-terminal tyrosines increased and extended the duration of its interaction with the myosin type 2. We further assessed the effects of HGAL on cell migration. Upon IL-6 stimulation for 24 hours, the migration of the Neo-HeLa cells in wound assay was markedly increased. However this effect of IL-6 was significantly ameliorated in the HGAL-HeLa cells. Moreover, siRNA knockdown of the HGAL in the HGAL-HeLa cells markedly reversed the HGAL-induced inhibition of IL-6 stimulated cell migration. This IL-6 effect on HGAL-HeLa and control Neo-HeLa cells was not attributable to differences in cell proliferation. Collectively, our results suggest that HGAL is involved in negative regulation of lymphocyte migration, thus constraining lymphocytes to the GC. Furthermore, inhibition of lymphocyte migration might contribute to the less aggressive clinical behavior of HGAL expressing DLBCL and HL tumors.

Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides

1995 ◽  
Vol 108 (4) ◽  
pp. 1617-1627 ◽  
Author(s):  
C. Rabouille ◽  
N. Hui ◽  
F. Hunte ◽  
R. Kieckbusch ◽  
E.G. Berger ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Hela Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Hela Cells ◽  
Stable Expression ◽  
Hela Cell Line ◽  
Specific Antibodies ◽  
Hela Cell Lines ◽  
Golgi Network

The distribution of beta 1,2 N-acetylglucosaminyltransferase I (NAGT I), alpha 1,3-1,6 mannosidase II (Mann II), beta 1,4 galactosyltransferase (GalT), alpha 2,6 sialyltransferase (SialylT) was determined by immuno-labelling of cryo-sections from HeLa cell lines. Antibody labelling in the HeLa cell line was made possible by stable expression of epitope-tagged forms of these proteins or forms from species to which specific antibodies were available. NAGT I and Mann II had the same distribution occupying the medial and trans cisternae of the stack. GalT and SialylT also had the same distribution but they occupied the trans cisterna and the trans-Golgi network (TGN). These results generalise our earlier observations on the overlapping distribution of Golgi enzymes and show that each of the trans compartments of the Golgi apparatus in HeLa cells contains unique mixtures of those Golgi enzymes involved in the construction of complex, N-linked oligosaccharides.

Effect of black seeds (Nigella sativa) volatile oil on the cervical cancer: In vitro study, on Hela cell lines

2019 ◽  
Vol 7 (4) ◽  
pp. 91-96
Author(s):  
Isra'a Al-sobhi ◽  
◽  
Rawan Al-Ghabban ◽  
Soad Shaker Ali ◽  
Jehan Al-Amri ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Cell Lines ◽  
Nigella Sativa ◽  
In Vitro Study ◽  
Volatile Oil ◽  
Vitro Study ◽  
Hela Cell Lines ◽  
Black Seeds

scholarly journals Structures, Chemical Conversions, and Cytotoxicity of Tricholopardins C and D, Two Tricholoma Triterpenoids from the Wild Mushroom Tricholoma pardinum

2021 ◽  
Author(s):  
Chen Shi ◽  
Yue-Ling Peng ◽  
Juan He ◽  
Zheng-Hui Li ◽  
Ji-Kai Liu ◽  
...  
Keyword(s):  
Hela Cell ◽  
Single Crystal ◽  
Cell Lines ◽  
Cell Apoptosis ◽  
Spectroscopic Methods ◽  
Wild Mushroom ◽  
X Ray Diffraction ◽  
X Ray ◽  
Hela Cell Lines ◽  
Mcf 7

AbstractTwo undescribed Tricholoma triterpenoids, namely tricholopardins C (1) and D (2), were isolated from the wild mushroom Tricholoma pardinum. Their structures with absolute configurations were elucidated by spectroscopic methods, as well as the single crystal X-ray diffraction. Compounds 1 and 2 were further obtained by chemical conversions from the known analogues. Compound 1 showed significant cytotoxicity to MCF-7 and Hela cell lines with IC50 values of 4.7 μM and 9.7 μM, respectively. Its mechanism of inducing MCF-7 cell apoptosis was studied briefly. Graphical Abstract

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