Biomimetic Apatite Deposition in Modified Dulbecco’s Phosphate-Buffered Saline Solution on the Alkali-Heat Treated TI6AL4V

Bioactive glasses have recently been extensively used to replace, regenerate, and repair hard tissues in the human body because of their ability to bond with living tissue. In this work, the effects of replacing Na2O with MgO on the electrical, biosolubility, and thermal properties of the target glass 10Na2O–60P2O5–30CaO (in mol%) were investigated. The electrical properties of the glasses were studied with the impedance spectroscopy technique. At 473 K, DC conductivity values decreased from 4.21 × 10−11 to 4.21 × 10−12 S cm−1 after complete substitution of MgO for Na2O. All samples had a similar activation energy of the DC conduction process ~1.27 eV. Conduction mechanisms were found to be due to hop of ions: Na+, Mg2+, and probable H+. FTIR analysis showed that, as the Mg content increased, the Q2 unit (PO2−) shifted towards higher wavenumbers. The proportion of Q3 unit (P2O5) decreased in the glass structure. This confirmed that the replacement of Na+ by Mg2+ was accompanied by concurrent polymerization of the calcium–phosphate glass network. The biosolubility test in the phosphate-buffered saline solution showed that the magnesium addition enhanced the biosolubility properties of Na2O–CaO–P2O5 glasses by increasing their dissolution rate and supporting forming CaP-rich layers on the surface. The glass transition temperature increased, and thermal stability decreased substantially upon substitution of Na2O by MgO.

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Survival Rate of Cells Sent by a Low Mechanical Load Tube Pump: The “Ring Pump”

Micromachines ◽

10.3390/mi11040447 ◽

2020 ◽

Vol 11 (4) ◽

pp. 447 ◽

Cited By ~ 1

Author(s):

Kaoru Uesugi ◽

Keizo Nishiyama ◽

Koki Hirai ◽

Hiroaki Inoue ◽

Yoichi Sakurai ◽

...

Keyword(s):

Survival Rate ◽

Cell Survival ◽

Saline Solution ◽

Mechanical Load ◽

Survival Rates ◽

Cell Culture System ◽

Large Size ◽

Cell Survival Rate ◽

Peristaltic Pumps ◽

Phosphate Buffered Saline

A ring pump (RP) is a useful tool for microchannels and automated cell culturing. We have been developing RPs (a full-press ring pump, FRP; and a mid-press ring pump, MRP). However, damage to cells which were sent by the RP and the MRP was not investigated, and no other studies have compared the damage to cells between RPs and peristaltic pumps (PPs). Therefore, first, we evaluated the damage to cells that were sent by a small size FRP (s-FRP) and small size MRPs (s-MRPs; gap = 25 or 50 μm, respectively). “Small size” means that the s-FRP and the s-MRPs are suitable for microchannel-scale applications. The survival rate of cells sent by the s-MRPs was higher than those sent by the s-FRP, and less damage caused by the former. Second, we compared the survival rate of cells that were sent by a large size FRP (l-FRP), a large size MRP (l-MRP) (gap = 50 μm) and a PP. “Large size” means that the l-FRP and the l-MRP are suitable for automated cell culture system applications. We could not confirm any differences among the cell survival rates. On the other hand, when cells suspended in Dulbecco’s phosphate-buffered saline solution were circulated with the l-MRP (gap = 50 μm) and the PP, we confirmed a difference in cell survival rate, and less damage caused by the former.

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Breaking strength and elasticity of synthetic absorbable suture materials incubated in phosphate-buffered saline solution, milk, and milk contaminated withStreptococcus agalactiae

American Journal of Veterinary Research ◽

10.2460/ajvr.68.4.441 ◽

2007 ◽

Vol 68 (4) ◽

pp. 441-445 ◽

Cited By ~ 8

Author(s):

Sylvain Nichols ◽

David E. Anderson

Keyword(s):

Saline Solution ◽

Breaking Strength ◽

Absorbable Suture ◽

Suture Materials ◽

Phosphate Buffered Saline

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SCANNING ION MICROBEAM ANALYSIS OF DIFFUSION IN IN-MOUTH DRUG RELEASE POLYMERS

International Journal of PIXE ◽

10.1142/s0129083505000477 ◽

2005 ◽

Vol 15 (03n04) ◽

pp. 161-167 ◽

Cited By ~ 2

Author(s):

G. MASSINGHAM ◽

R. ARSLANOGLU ◽

F. E. GAUNTLETT ◽

M. S. RIHAWY ◽

R. W. SMITH ◽

...

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Nuclear Reaction ◽

Drug Delivery Systems ◽

Saline Solution ◽

Pure Water ◽

X Ray ◽

Preliminary Results ◽

Water Infusion ◽

Phosphate Buffered Saline

With the aim of characterizing polymer-based drug delivery systems a combination of Scanning MeV 3 He microbeam Nuclear Reaction, Backscattering and Particle Induced X-ray Emission (PIXE) techniques has been developed. This, together with gravimetric and UV techniques has been applied to characterize both water infusion and drug effusion for three in-mouth polymer–drug systems. Preliminary results are presented from the exposure of polymers, containing drug at a level of 9% by weight of the dry polymer, to both pure water and a phosphate buffered saline solution at 37°C.

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An Anti-Inflammatory Poly(PhosphorHydrazone) Dendrimer Capped with AzaBisPhosphonate Groups to Treat Psoriasis

Biomolecules ◽

10.3390/biom10060949 ◽

2020 ◽

Vol 10 (6) ◽

pp. 949 ◽

Cited By ~ 3

Author(s):

Ranime Jebbawi ◽

Abdelouahd Oukhrib ◽

Emily Clement ◽

Muriel Blanzat ◽

Cédric-Olivier Turrin ◽

...

Keyword(s):

Topical Application ◽

Therapeutic Efficacy ◽

Biomedical Applications ◽

Saline Solution ◽

Active Pharmaceutical Ingredient ◽

Systemic Injection ◽

Human Immune ◽

Anti Inflammatory ◽

Phosphate Buffered Saline

Dendrimers are nanosized, arborescent macromolecules synthesized in a stepwise fashion with attractive degrees of functionality and structure definition. This is one of the reasons why they are widely used for biomedical applications. Previously, we have shown that a poly(phosphorhydrazone) (PPH) dendrimer capped with anionic azabisphosphonate groups (so-called ABP dendrimer) has immuno-modulatory and anti-inflammatory properties towards human immune cells in vitro. Thereafter, we have shown that the ABP dendrimer has a promising therapeutic efficacy to treat models of acute and chronic inflammatory disorders in animal models. In these models, the active pharmaceutical ingredient was administered systematically (intravenous and oral administrations), but also loco-regionally in the vitreous tissue. Herein, we assessed the therapeutic efficacy of the ABP dendrimer in the preclinical mouse model of psoriasis induced by imiquimod. The ABP dendrimer was administered in phosphate-buffered saline solution via either systemic injection or topical application. We show that the topical application enabled the control of both the clinical and histopathological scores, and the control of the infiltration of macrophages in the skin of treated mice.

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Occurrence of Yersinia enterocolitica in Poultry Products

Journal of Food Protection ◽

10.4315/0362-028x-45.4.322 ◽

1982 ◽

Vol 45 (4) ◽

pp. 322-325 ◽

Cited By ~ 27

Author(s):

E. DE BOER ◽

B. J. HARTOG ◽

J. OOSTEROM

Keyword(s):

Yersinia Enterocolitica ◽

Bile Salts ◽

Potassium Hydroxide ◽

Saline Solution ◽

Potassium Hydroxide Solution ◽

Macconkey Agar ◽

Hydroxide Solution ◽

Poultry Products ◽

Phosphate Buffered Saline

In a study on isolation of Yersinia enterocolitica from poultry products, usefulness of the following enrichment media was evaluated: phosphate-buffered saline solution with and without addition of 1% sorbitol plus 0.15% bile salts, modified Rappaport medium and selenite broth. Plating was performed on MacConkey agar directly from the incubated broths and after treatment of the enrichment broths with a potassium hydroxide solution. Seventy-three of 108 (68%) samples of poultry products contained Y. enterocolitica. Y. enterocolitica was isolated most frequently after enrichment in phosphate-buffered saline solution with sorbitol and bile salts. Nearly all Y. enterocolitica strains were found after KOH-treatment. Serotyping showed that the isolated strains mainly belonged to the category of the non-pathogenic, so called “environmental” strains.

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