scholarly journals Hydrocarbon-Degrading Bacterial Strain Pseudomonas mendocina Newly Isolated from Marine Sediments and Seawater of Oran Harbor (Algerian Coast)

2020 ◽  
Vol 2 (2) ◽  
pp. 22-29
Author(s):  
Bendadeche Medjahed Faiza
Keyword(s):  
Energy Source ◽  
Growth Rate ◽  
Crude Oil ◽  
16S Rdna ◽  
Marine Sediments ◽  
Growth Kinetic ◽  
Culture Conditions ◽  
Pseudomonas Mendocina ◽  
Degrading Bacterium ◽  
Nacl Concentration

Contamination by petroleum hydrocarbons causes serious dangers to human health and the environment, whether by accidental or chronic contamination. Due to the large flow of ships, the commercial harbor of Oran is subject to pollution particularly by polycyclic aromatic hydrocarbons. For that, bioremediation by indigenous microorganisms is the most important method to eliminate or decrease this contamination. In the present paper, hydrocarbon-degrading bacterium strain SP57N has been studied, newly isolated from contaminated marine sediments and sea water from the harbor of Oran (Northwestern-Algeria), using of Bushnell-Hass salt medium (BHSM). The strain SP57N was Gram-negative, oxidase negative, catalase negative, motile, Rod-shaped bacteria, identified molecularly as Pseudomonas mendocina based on partial 16S rDNA gene sequence analysis, using the BLAST program on National Centre for Biotechnology Information (NCBI) and the EzBioCloud 16S rDNA databases. This isolate could growth on high concentrations of crude oil (up to 10 %, v/v). The effects of some culture conditions such as temperature, NaCl concentration and pH on growth rate of strain SP57N on crude oil as the sole carbon and energy source were studied. In addition, growth kinetic of this isolate on crude oil during 20 days of culture at 140 rpm, under optimal culture conditions was considered. The results showed maximum growth rate at temperature 25°C, 3% (w/v) of NaCl concentration and pH 7. Results of growth kinetic on crude oil as sole carbon and energy source showed that the stationary phase was attained at day 12. Thus, Pseudomonas mendocina SP57N had effectively hydrocarbon-degrading potential, and could be used as an efficacy degrader to initiate a biological eco-friendly method for the bioremediation of the hydrocarbon pollution on the port of Oran, and marine environment.

Isolation, identification and in silico study of native cellulase producing bacteria

2019 ◽  
Vol 17 ◽  
Author(s):  
Farzane Kargar ◽  
Mojtaba Mortazavi ◽  
Mahmood Maleki ◽  
Masoud Torkzadeh Mahani ◽  
Younes Ghasemi ◽  
...  
Keyword(s):  
Growth Rate ◽  
Bacillus Cereus ◽  
16S Rdna ◽  
Enzyme Production ◽  
Rational Design ◽  
Cellulase Production ◽  
Bacillus Species ◽  
Bacillus Sp ◽  
Chain Polymer ◽  
Bacillus Toyonensis

Aims: The purpose of this study was to screen the bacteria producing cellulase enzymes and their bioinformatics studies. Background: Cellulose is a long-chain polymer of glucose that hydrolyzes by cellulases to glucose molecules. In order to design the new biotechnological applications, some strategies have been used as increasing the efficiency of enzyme production, generating cost-effective enzymes, producing stable enzymes and identification of new strains. Objective: On the other hand, some bacteria special features have made them suitable candidates for the identification of the new source of enzymes. In this regard, some native strains of bacteria were screened. Method: These bacteria were grown on a culture containing the liquid M9 media containing CMC to ensure the synthesis of cellulase. The formation of a clear area in the culture medium indicated decomposition of cellulose. In the following, the DNA of these bacteria were extracted and their 16S rDNA genes were amplified. Result: The results show that nine samples were able to synthesize cellulase. In following, these strains were identified using 16S rDNA. The results show that these screened bacteria belonged to the Bacillus sp., Alcaligenes sp., Alcaligenes sp., and Enterobacter sp.conclusionThe enzyme activity analysis shows that the Bacillus toyonensis, Bacillus sp. strain XA15-411 Bacillus cereus have produced the maximum yield of cellulases. However, these amounts of enzyme production in these samples are not proportional to their growth rate. As the bacterial growth chart within 4 consecutive days shows that the Alcaligenes sp. Bacillus cereus, Bacillus toyonensis, Bacillus sp. strain XA15-411 have a maximum growth rate. The study of the phylogenetic tree also shows that Bacillus species are more abundant in the production of cellulase enzyme. These bioinformatics analyses show that the Bacillus species have different evolutionary relationships and evolved in different evolutionary time. Other: However, for maximum cellulase production by this bacteria, some information as optimum temperature, optimum pH, carbon and nitrogen sources are needed for the ideal formulation of media composition. The cellulase production is closely controlled in microorganisms and the cellulase yields appear to depend on a variety of factors. However, the further studies are needed for cloning, purification and application of these new microbial cellulases in the different commercial fields as in food, detergent, and pharmaceutical, paper, textile industries and also various chemical industries. However, these novel enzymes can be further engineered through rational design or using random mutagenesis techniques.

scholarly journals Factors Influencing the Chlorine Susceptibility of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum

2003 ◽  
Vol 69 (9) ◽  
pp. 5685-5689 ◽  
Author(s):  
Joseph O. Falkinham
Keyword(s):  
Growth Rate ◽  
Mycobacterium Avium ◽  
Growth Phase ◽  
Growth Rates ◽  
Growth Stage ◽  
Tween 80 ◽  
Culture Conditions ◽  
C Cells ◽  
Mycobacterium Intracellulare ◽  
Factors Influencing

ABSTRACT The susceptibility of representative strains of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (the MAIS group) to chlorine was studied to identify factors related to culture conditions and growth phase that influenced susceptibility. M. avium and M. intracellulare strains were more resistant to chlorine than were strains of M. scrofulaceum. Transparent and unpigmented colony variants were more resistant to chlorine than were their isogenic opaque and pigmented variants (respectively). Depending on growth stage and growth rate, MAIS strains differed in their chlorine susceptibilities. Cells from strains of all three species growing in early log phase at the highest growth rates were more susceptible than cells in log and stationary phase. Rapidly growing cells were more susceptible to chlorine than slowly growing cells. The chlorine susceptibility of M. avium cells grown at 30°C was increased when cells were exposed to chlorine at 40°C compared to susceptibility after exposure at 30°C. Cells of M. avium grown in 6% oxygen were significantly more chlorine susceptible than cells grown in air. Chlorine-resistant MAIS strains were more hydrophobic and resistant to Tween 80, para-nitrobenzoate, hydroxylamine, and nitrite than were the chlorine-sensitive strains.

scholarly journals Bacterial population and biodegradation potential in chronically crude oil-contaminated marine sediments are strongly linked to temperature

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Rafael Bargiela ◽  
Francesca Mapelli ◽  
David Rojo ◽  
Bessem Chouaia ◽  
Jesús Tornés ◽  
...  
Keyword(s):  
Crude Oil ◽  
Marine Sediments ◽  
Bacterial Population ◽  
Contaminated Marine Sediments

scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant

2019 ◽  
Vol 4 (1) ◽  
pp. 79-88
Author(s):  
Evi Octaviany ◽  
Suharjono Suharjono ◽  
Irfan Mustafa
Keyword(s):  
Crude Oil ◽  
16S Rdna ◽  
Bacterial Isolates ◽  
High Concentration ◽  
Isolation And Identification ◽  
Rdna Sequences ◽  
Degrading Bacteria ◽  
16S Rdna Sequences ◽  
Petroleum Contaminated Soil ◽  
Cell Densities

A commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study. 

Identification and characterization of a cold-adapted and halotolerant nitrobenzene-degrading bacterium

RSC Advances ◽  
2015 ◽  
Vol 5 (98) ◽  
pp. 80276-80282 ◽  
Author(s):  
Xiping Ma ◽  
Xianzhu Li ◽  
Wanlong Li ◽  
Di Wang ◽  
Chengbin Xu ◽  
...  
Keyword(s):  
16S Rdna ◽  
Gene Sequence ◽  
16S Rdna Gene ◽  
Cold Adapted ◽  
Biochemical Characteristics ◽  
Physiological And Biochemical Characteristics ◽  
Degrading Bacterium ◽  
Identification And Characterization ◽  
Physiological And Biochemical

According to strain X7's morphological, physiological and biochemical characteristics and 16S rDNA gene sequence, the result showed that strain X7 was Myroides odoratus.

Community-Based 16S rDNA Fingerprinting Analysis of Geographically Distinct Marine Sediments of Unexplored Coastal Regions of Palk Bay and Gulf of Mannar

2022 ◽  
Vol 79 (2) ◽  
Author(s):  
Chairmandurai Aravindraja ◽  
Dharmaprakash Viszwapriya ◽  
Alaguvel Valliammai ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
16S Rdna ◽  
Marine Sediments ◽  
Gulf Of Mannar ◽  
Coastal Regions ◽  
Community Based ◽  
Fingerprinting Analysis ◽  
Palk Bay

scholarly journals Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘Brevibacterium helvolum’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov.

2004 ◽  
Vol 54 (3) ◽  
pp. 783-789 ◽  
Author(s):  
Célia M. Manaia ◽  
Balbina Nogales ◽  
Norbert Weiss ◽  
Olga C. Nunes
Keyword(s):  
Cell Wall ◽  
16S Rdna ◽  
Sequence Similarity ◽  
Rdna Sequence ◽  
Strictly Aerobic ◽  
16S Rdna Sequence ◽  
Cell Wall Peptidoglycan ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacterium ◽  
Phylogenetic Level

A Gram-positive, molinate-degrading bacterium, strain ON4T (=DSM 13485T=LMG 21909T), was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of 10–41 °C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on d-ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent in the family Microbacteriaceae, showing the highest 16S rDNA similarity (∼95 %) to members of the genus Curtobacterium and ‘Brevibacterium helvolum’ DSM 20419 (=ATCC 13715). The latter was reported to have the cell-wall peptidoglycan type B2γ and the major menaquinone MK-9, which are typical of Clavibacter, but it is clearly separated from this genus at the phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously described genera and their distinctive phenotypic characteristics, it is proposed that strains ON4T and ‘B. helvolum’ DSM 20419 be classified as two novel genera and species, with the respective names Gulosibacter molinativorax gen. nov., sp. nov. and Pseudoclavibater helvolus gen. nov., sp. nov.

scholarly journals Complete Genome Sequence of the Marine Hydrocarbon Degrader Alcaligenes aquatilis QD168, Isolated from Crude Oil-Polluted Sediment of Quintero Bay, Central Chile

2019 ◽  
Vol 8 (5) ◽  
Author(s):  
Roberto E. Durán ◽  
Bárbara Barra-Sanhueza ◽  
Francisco Salvà-Serra ◽  
Valentina Méndez ◽  
Daniel Jaén-Luchoro ◽  
...  
Keyword(s):  
Crude Oil ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Central Chile ◽  
Coding Sequences ◽  
Content Type ◽  
Polluted Sediment ◽  
Degrading Bacterium ◽  
Hydrocarbon Degrader

Alcaligenes aquatilis strain QD168 (= CCUG 69566) is a marine hydrocarbon-degrading bacterium isolated from crude oil-polluted sediment from Quintero Bay, Central Chile. Here, we present the 4.32-Mb complete genome sequence of strain QD168, with 3,892 coding sequences, 58 tRNAs, and a 56.3% G+C content.

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