scholarly journals Rhizobium phaseoli tolerant to insecticide on the growth of Phaseolus vulgaris

2021 ◽  
Vol 12 (1) ◽  
pp. 30-37
Author(s):  
Gustavo Santoyo-Pizano ◽  
José Luis Hernández-Mendoza ◽  
Liliana Márquez-Benavides ◽  
Gustavo De Luna-Esquivel ◽  
Juan Manuel Sánchez-Yáñez
Keyword(s):  
Phaseolus Vulgaris ◽  
Beneficial Effect ◽  
Root Nodules ◽  
Molecular Nitrogen ◽  
Dry Weight ◽  
Rhizobium Phaseoli ◽  
Control Root ◽  
Healthy Growth

The genus and species of Rhizobium phaseoli are useful as an inoculant for the production of Phaseolus vulgaris (beans) in the root nodules symbiotic stage fixes molecular nitrogen (FN) for supplying nitrogen (N) for healthy growth. In P. vulgaris cropping, pesticides are used to control root insects, which could reduce the beneficial effect of R. phaseoli. The purpose of this work was to isolate and select R. phaseoli diazinon´s tolerance. In that sense, R. phaseoli were cultivated in a medium mixed with diazinon in order to select R. phaseoli tolerant to the pesticide. This mutant of R. phaseoli tolerant to diazinon was inoculated in P. vulgaris the effect was evaluated 45 days later. The response of P. vulgaris was measured by the number of effective nodules at the roots, fresh and dry weight, and the height of the plant. Results showed that R. phaseoli tolerant to diazinon kept its beneficial activity for the healthy growth of P. vulgaris. It was concluded that R. phaseoli tolerant to diazinon were infec-tive and effective for the health growth of P. vulgaris.

scholarly journals N2 FIXATION AND COMPETITIVENESS OF Rhizobium phaseoli STRAINS ISOLATED FROM ONTARIO SOILS

1986 ◽  
Vol 66 (4) ◽  
pp. 825-836 ◽  
Author(s):  
JACQUELINE C. MOXLEY ◽  
D. J. HUME ◽  
D. L. SMITH
Keyword(s):  
Phaseolus Vulgaris ◽  
Effective Strain ◽  
Dry Weight ◽  
Field Trials ◽  
Rhizobium Phaseoli ◽  
Culture Collections ◽  
N Accumulation ◽  
Legume Crop ◽  
Wide Range ◽  
White Bean

Dry bean (Phaseolus vulgaris L.) is a legume crop generally considered to be a poor N2 fixer. This experiment was conducted to determine the effectiveness of Rhizobium phaseoli strains in Ontario soils, to determine if inoculation with an indigenous, effective strain could improve N2 fixation or yield, and to determine the competitiveness of inoculant strains by measuring the recovery of applied strains in nodules using serology. One-gram composite soil samples from 36 of 37 farm sites across Ontario caused nodulation on either white bean cultivars Ex Rico 23 or Seafarer, indicating that R. phaseoli was widely distributed. However, ratings of nodulation indicated a wide range of effectiveness. Five selected strains isolated from these soils were compared with five strains from culture collections for effectiveness by using the strains to inoculate Ex Rico 23 or Seafarer grown in sterile, N-free culture in Leonard jars. The best field-isolated strains caused just as much N accumulation as the best strains from culture collections. Strain S1, selected as a consistently good N2 fixer, failed to cause increases in yield when used as an inoculant in field trials at Elora and Mitchell in 1980 and 1981. Inoculation with strain S1 did cause some increases in percent seed protein and nodule dry weight with Ex Rico 23 at Elora in 1980. Three other strains tested in each year failed to show any improvements in yield, seed N, or nodulation in either year. Recovery of inoculant strains ranged from 0 to 31%. At Mitchell in 1981, 100 kg ha−1 of fertilizer N increased yields over all inoculation treatments. Indigenous strains appeared to be only partially effective N2 fixers and inoculant strains generally were poor competitors against strains already present in the soils.Key words: White bean, Phaseolus vulgaris, Rhizobium phaseoli, N2 fixation, strain competition

scholarly journals Mutation in the ntrR Gene, a Member of the vap Gene Family, Increases the Symbiotic Efficiency of Sinorhizobium meliloti

2001 ◽  
Vol 14 (7) ◽  
pp. 887-894 ◽  
Author(s):  
Boglárka Oláh ◽  
Erno Kiss ◽  
Zoltán Györgypál ◽  
Judit Borzi ◽  
Gyöngyi Cinege ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Pathogenic Bacteria ◽  
Sinorhizobium Meliloti ◽  
Root Nodules ◽  
Nifh Gene ◽  
Dry Weight ◽  
Nodule Occupancy ◽  
Gene Encoding ◽  
Symbiotic Efficiency ◽  
Host Interactions

In specific plant organs, namely the root nodules of alfalfa, fixed nitrogen (ammonia) produced by the symbiotic partner Sinorhizobium meliloti supports the growth of the host plant in nitrogen-depleted environment. Here, we report that a derivative of S. meliloti carrying a mutation in the chromosomal ntrR gene induced nodules with enhanced nitrogen fixation capacity, resulting in an increased dry weight and nitrogen content of alfalfa. The efficient nitrogen fixation is a result of the higher expression level of the nifH gene, encoding one of the subunits of the nitrogenase enzyme, and nifA, the transcriptional regulator of the nif operon. The ntrR gene, controlled negatively by its own product and positively by the symbiotic regulator syrM, is expressed in the same zone of nodules as the nif genes. As a result of the nitrogen-tolerant phenotype of the strain, the beneficial effect of the mutation on efficiency is not abolished in the presence of the exogenous nitrogen source. The ntrR mutant is highly competitive in nodule occupancy compared with the wild-type strain. Sequence analysis of the mutant region revealed a new cluster of genes, termed the “ntrPR operon,” which is highly homologous to a group of vap-related genes of various pathogenic bacteria that are presumably implicated in bacterium-host interactions. On the basis of its favorable properties, the strain is a good candidate for future agricultural utilization.

scholarly journals Isolation and Characterization of Nodule-AssociatedExiguobacterium sp. from the Root Nodules ofFenugreek(Trigonella foenum-graecum) and Their Possible Role in Plant Growth Promotion

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Geetha Rajendran ◽  
Maheshwari H. Patel ◽  
Sanket J. Joshi
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Root Nodules ◽  
Dry Weight ◽  
Gram Positive ◽  
Associated Bacteria ◽  
Trigonella Foenum Graecum ◽  
Isolation And Characterization ◽  
Root And Shoot Length

One of the ways to increase the competitive survivability of rhizobial biofertilizers and thus achieve better plant growth under such conditions is by modifying the rhizospheric environment or community by addition of nonrhizobial nodule-associated bacteria (NAB) that cause better nodulation and plant growth when coinoculated with rhizobia. A study was performed to investigate the most commonly associated nodule-associated bacteria and the rhizospheric microorganisms associated with theFenugreek(Trigonella foenum-graecum) plant. Isolation of nonrhizobial isolates from root nodules ofFenugreekwas carried out along with the rhizospheric isolates. About 64.7% isolates obtained fromFenugreeknodules were gram-negative coccobacilli, 29.41% were gram-positive bacilli, and all rhizospheric isolates except one were gram-positive bacilli. All the isolates were characterized for their plant growth promoting (PGP) activities. Two of the NAB isolates M2N2c and B1N2b (Exiguobacterium sp.) showed maximum positive PGP features. Those NAB isolates when coinoculated with rhizobial strain—S. meliloti, showed plant growth promotion with respect to increase in plant’s root and shoot length, chlorophyll content, nodulation efficiency, and nodule dry weight.

scholarly journals Oxidation of glycine by Phaseolus leghaemoglobin with associated catabolic reactions at the haem

1978 ◽  
Vol 176 (2) ◽  
pp. 351-358 ◽  
Author(s):  
P Lehtovaara
Keyword(s):  
Hydrogen Peroxide ◽  
Carbon Monoxide ◽  
Superoxide Dismutase ◽  
Phaseolus Vulgaris ◽  
Initial Velocity ◽  
Root Nodules ◽  
Green Product ◽  
Oxygen Complex ◽  
Optimum Ph ◽  
Glycine Oxidase

Leghaemoglobin from the root nodules of kidney bean (Phaseolus vulgaris) reacts in alkaline glycine solutions as a glycine oxidase in a reaction that may also be regarded as a coupled oxidation. Leghaemoglobin is reduced to the ferrous form by glycinate, the oxygen complex is formed, and finally the haem is attacked to yield a green reaction product. Glycine is simultaneously oxidized to glyoxylate, and hydrogen peroxide is generated. The initial velocity of the formation of the green product is proportional to the concentrations of leghaemoglobin and glycine, and the optimum pH for the reaction is 10.2. The green product is not formed if carbon monoxide, azide of imidazole is bound to the haem, whereas oxidation of glycine to glyoxylate is not inhibited by azide and not essentially by carbon monoxide. Haem breakdown is activated by digestion of leghaemoglobin by carboxypeptidase, and partly inhibited by catalase and superoxide dismutase.

scholarly journals Influence of boron on growth and mineral composition of bean (Phaseolus vulgaris L.)

1981 ◽  
Vol 38 (1) ◽  
pp. 309-318
Author(s):  
I. P. Oliveira ◽  
E. Malavolta
Keyword(s):  
Phaseolus Vulgaris ◽  
Mineral Composition ◽  
Nutrient Solution ◽  
Boron Concentration ◽  
Dry Weight ◽  
Phaseolus Vulgaris L ◽  
Mineral Concentration ◽  
Affected Plant ◽  
Differential Behaviour ◽  
Total Dry Weight

Nine cultivars of Phaseolus vulgaris L. were grown in nutrient solution to study the effect of boron on growth and mineral composition. Data obtained in thie study allowed for the following conclusions: (1) high levels of boron affected plant height, root length, dry weight of tops, dry weight of root, and total dry weight; (2) regression analysis was used to point out differential behaviour among cultivars in relation to boron concentration in nutrient solution; (3) the best mineral concentration in the plant tissue was obtained with application of 0,5 ppm of boron in the nutrient solution.

scholarly journals Pertumbuhan Semai Sengon (Paraserianthes Falcataria (L.) Nielsen) Pada Media Tanah Bekas Tambang Kapur Dengan Penambahan Pupuk Kompos Dan NPK

2019 ◽  
Vol 10 (1) ◽  
pp. 51-57
Author(s):  
Basuki Wasis ◽  
Siti Halimatus Sa'idah
Keyword(s):  
Seedling Growth ◽  
Root Nodules ◽  
Dry Weight ◽  
Soil Condition ◽  
Major Effect ◽  
Paraserianthes Falcataria ◽  
Soil Medium ◽  
Mining Sites ◽  
Randomized Design ◽  
Npk Fertilizer

Limestone mining activities are damaging soil and enviroment. Revegetation becomes an obligation on every mining sites. Paraserianthes falcataria is one of many species that suitable to be planted in revegetation site. Adding compost and NPK fertilizer can improve soil’s condition. This research objective is to analyze the effect of compost and NPK fertilizer on Paraserianthes falcataria seedlings growth and the exact appropriate dosage for limestone mining’s soil condition improvement. The design used in this research is completely randomized design with two factors. The result shows that addtion of compost on tailing media doesn’t give major effect or statistically not significant on seedling growth of height, diameter, the amount of root nodules, and shoot-root ratio, but statistically significant on wet and dry weight of the seedlings. NPK fertilizer addition on tailing media is statistically significant on the seedling growth of diameter, wet weight, and shoot-root ratio, but not significant different on height, dry weight, and the amount of root nodules. Interaction between compost and NPK fertilizer on media gives effect only at 95% of confidence level. K1N5 sample (with 100 gram composts and 5 gram of NPK fertilizer) gives the best respond on seedling’s growth. Very high levels of Ca in the soil medium will be toxic to the Paraserianthes Falcataria plant.Keywords: Compost, limestone mining, NPK fertilizer, Paraserianthes falcataria

scholarly journals Influence of Soil Amendment on the Relative Growth Rate and Net Assimilation Rate of Phaseolus vulgaris and Vigna aconitifolia

2020 ◽  
pp. 1-13
Author(s):  
E. A. Effa ◽  
A. A. J. Mofunanya ◽  
B. A. Ngele
Keyword(s):  
Growth Rate ◽  
Phaseolus Vulgaris ◽  
Relative Growth Rate ◽  
Soil Amendment ◽  
Dry Weight ◽  
Organic Manure ◽  
Assimilation Rate ◽  
Vigna Aconitifolia ◽  
Relative Growth ◽  
Net Assimilation

Background: Soil pH is one of the most important factors that contribute to crop growth and productivity. The present research was designed to assess the influence of soil amendment using organic manure and agricultural lime on the relative growth rate (RGR) and net assimilation rate (NAR) of Phaseolus vulgaris and Vigna aconitifolia grown on soils from different locations. Methodology: The three locations were: Akamkpa, Calabar Municipality and Odukpani. The pH for the three soil locations were 4.0, 7.0 and 9.0, respectively. The treatments were; control (0 g), OM1 (100 g organic manure), OM2 (200 g organic manure), AL1 (100 g agricultural lime), AL2 (200 g agricultural lime), OM1 + AL1 (50 g organic manure + 50 g agricultural lime) and OM2 +AL2 (100 g organic manure and 100 g agricultural lime). Results: Results obtained on the RGR of the leaf dry weight of P. vulgaris treated with OM2 was the highest (0.50 g/wk) followed by OM1 (0.41 g/wk). OM1 + AL1 had the highest RGR of the stem dry weight of P. vulgaris grown on soil from Calabar Municipality. In the RGR of the root dry weight, OM2 had the highest mean value in both plants grown on Akamkpa soil. Results obtained at 4 weeks after planting (WAP) revealed that there was significant (P<0.05) increase in NAR of plants grown on soil from Akamkpa. The highest NAR was obtained for V. aconitifolia treated with OM2 (0.0447 g/wk) followed by OM2 + AL2 (0.0057 g/wk) for both V. aconitifolia and P. vulgaris. P. vulgaris grown on Akamkpa and Odukpani soils treated with AL2 (0.0032 g/wk), OM1 + AL1 (0.0041 g/wk) and OM2+ AL2 (0.0062 g/wk) had the highest NAR at 8 WAP. Conclusion: The RGR and NAR of the two bean varieties were improved following treatments with organic manure and agricultural lime.

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